Protein Alignment HUWE1 and Nedd4

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: XP_017450968.1
• Gene: Nedd4 / 25489
• RGDID: 3157
• Length: 1223
• Species: Rattus norvegicus
• Alignment Length: 1341
• Identity: 343/1341 - (25%)
• Similarity: 525/1341 - (39%)
• Gaps: 364/1341 - (27%)

  Fly  3978 EGSARSISINPQAAQMIVRNCLDLLFVLAKHYPSSFVPFNRDVKARRILSAVFGMPSPTAGSGG- 4041
            :.|...||:.|:..     .||          ||..|..:||     .:|....:......|.|
  Rat    73 KSSCLQISLQPRYG-----GCL----------PSGTVVVDRD-----DISFTCALKDSVCSSTGV 117

  Fly  4042 GAGSGSAASGGLTGYDDVPPGGTLDFRSRFCRYQVVNRLRSIFDDHPAPKPSVPLDEAPSTSKAA 4106
            .:...||:.|.|.|...:..|...:|              |..|:          ....|.....
  Rat   118 DSEVSSASDGSLLGISAMYNGSLSNF--------------SASDN----------GSYSSNGSDC 158

  Fly  4107 AAKAAATAKVSTAGSQNKHSVPYQTNAKNFWDVVLNIDQQTQVRLANVAQFPLTSQPAWEWQPNT 4171
            .:.|:.|:..|..||....|..|:|    |:...|..|..:.           .|.|.     ||
  Rat   159 GSCASLTSGCSYTGSVVSDSSGYET----FFSGNLPSDNTSN-----------RSVPV-----NT 203

  Fly  4172 ADFLSFSESPFGQLLEMLPYKVICRSPHLTDMLVKLLASLSTELPKEEELLPLSDQMPPLVPIG- 4235
            |                 |.:|:||||              :.||..:|.|....::.. ||:.
  Rat   204 A-----------------PREVLCRSP--------------SRLPFAQEDLEHGVEIMK-VPVSG 236

  Fly  4236 ----PVQRAGAAAAAAHPPPPQPPAENTAT-ATEIGDGLAANIEEQASPASGNPASGDSNAGAKR 4295
                |::|..:.......|...|||..|:. |.:....|..:..|.|....|....|..:.....
  Rat   237 NAKVPLKRCTSLVIFPRSPSNTPPASPTSRGAYQTSHQLVVSPTETAHHEDGTGTRGFLSTAVSG 301

  Fly  4296 TQLPTLSVALPVTPSKPR--RKIYSKNFSQLQLVIEVLTHQCGTTEGL-DNVAKLIVNLTQCSQA 4357
            .:|..::    .||.:.|  |.:::|...|.::||.....:....|.| :..:.:.|:|||.:|.
  Rat   302 LRLSKVT----CTPGEVRDVRPLHAKGSLQKKIVISDNRPKQTVCEKLPERFSCVSVHLTQQNQT 362

  Fly  4358 SSAIFIQYLTAAILGLAEEVRQAIQMLLNEIRMYNIHQGGAGTTSQAAS---------------- 4406
            :|    ......:...:|.:...:|..|:.           |..:|.||
  Rat   363 AS----DCANPCVGRTSERLELQLQSDLDH-----------GMLTQRASPCLPTTPDTDSHVNIR 412

  Fly  4407 ----------TSGAAAATASY-----VPTVSGASLLPNLAVHEGIMQ-------------DRFTA 4443
                      :.|.||...|.     .|.||..|.|.:.....|..|             |.|..
  Rat   413 GELERPNSQRSRGRAALQRSVSLGGTYPNVSCLSSLKHSCSKGGPSQLLIKFASGNDGKVDSFWR 477

  Fly  4444 EHVIISAPKNAKPTCE----------LQLPSMKKLMSNSSAQPYFLR-----------TLKIFMQ 4487
            |..:.:.....:.|.:          ..||:....|.    :||..:           .:|.:::
  Rat   478 ERDLTNELSGVRKTRDDFLGQVDVPLYPLPTENPRME----RPYTFKDFVLHPRSHKSRVKGYLR 538

  Fly  4488 VRDMYEAQNGLSTGGGVACGAGDNDDDIIDIEGDGGASGGSSSDMAPG-------SAST------ 4539
            ::..|..:||             :||:..|          .:.::.||       .|:|
  Rat   539 LKMTYLPKNG-------------SDDENAD----------QAEELEPGWVVLDQPDAATHLQHPP 580

  Fly  4540 -ISPPPP------------------METNQIAQDGDDDEEDDDE--------ARSF--------- 4568
             .||.||                  ..|.|..:...:|:..|||        .|:|
  Rat   581 EPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRPSPEDDLTDDENGDIQLQAQRAFTTRRQISED 645

  Fly  4569 -PGVGNHAVASASKSTKPTLSQILCLDVLWNTL-------------SECLVALEESKDEFAVLVL 4619
             .|..||        ..|...:|:..|.  ||:             .:..|.|.|..|....:..
  Rat   646 VDGPDNH--------ESPENWEIVREDE--NTMYSGQAVQSPPSGHPDVQVRLAEELDTRLTMYG 700

  Fly  4620 QP-TVEAFFLIHASHRGASKKSG--------RSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQE- 4674
            .| |.:.....:.|.||.|.::.        ...:|.::|.                 .|..:|
  Rat   701 NPATSQPVTSSNHSSRGGSSQTCIFEEQPTLPVLLPTSSGL-----------------PPGWEEK 748

  Fly  4675 -----YSPFMDDVSSANTPSSASFDDEPRMQ---DASTNTPLVTNPSTSTAMSAHEAQLRQDRQK 4731
                 .|.::|..|...|.|..:..|:||.:   .....||:.:| .........|.:...|.:.
  Rat   749 QDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTPVDSN-DLGPLPPGWEERTHTDGRV 812

  Fly  4732 FLQFAEKHRTVLNQILRQSPTHLSD---------GPFAVLVDHTRILDFDVKRKY--FQTELERL 4785
            |.         :|..:::  |...|         ||   .|.::|    |.||||  |:.:|::.
  Rat   813 FF---------INHNIKK--TQWEDPRMQNVAITGP---AVPYSR----DYKRKYEFFRRKLKKQ 859

  Fly  4786 DEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEW-KNRFYIVFEDEEGQDAGGLLREWYVIISRE 4849
            .:...:.|  :.:||..:.|||:|.:..:...:: |.|.:|.|:.|:|.|.||:.|||:.:||:|
  Rat   860 TDIPNKFE--MKLRRANILEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKE 922

  Fly  4850 IFNPMYALFCVSPGDRVTYMINPSSH-ANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHI 4913
            :|||.|.||..|..|..|..|||:|. .|.:|||||||:|||...||:..|||:.:|.|.|||.:
  Rat   923 MFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMM 987

  Fly  4914 LGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEE 4978
            |.|.:...||||.|.|:|..|.::::||.:.|  :|.|..:.:.||.|...:||..|.:..||.:
  Rat   988 LQKLITLHDMESVDSEYYSSLRWILENDPTEL--DLRFIIDEELFGQTHQHELKTGGSEVVVTNK 1050

  Fly  4979 NKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANT 5043
            ||.||:.||.|.:....|::|:.||.|||:::||:.||.||:|.|||||:.||.|:|:.|.:.:|
  Rat  1051 NKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHT 1115

  Fly  5044 EY-HKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDR 5107
            :| :.|:.....|.|||:|:...|...|.:.||||||||:||:.||..|.|.||.|.|.:.:..
  Rat  1116 KYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWG- 1179

  Fly  5108 STDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGF 5148
            :.|:||.||||||:||||.|:|:|:|...|..|| |.::||
  Rat  1180 TPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAI-ENTQGF 1219

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	160/356 (45%)
Nedd4	XP_017450968.1	C2	<489..544	CDD:472691	7/58 (12%)
		WW	588..616	CDD:238122	2/27 (7%)
		WW	741..770	CDD:459800	7/45 (16%)
		WW	797..829	CDD:197736	6/42 (14%)
		HECTc	890..1219	CDD:214523	152/332 (46%)