Protein Alignment HUWE1 and pub2

DIOPT Version :10

• Sequence 1: NP_001285288.1
• Gene: HUWE1 / 32510
• FlyBaseID: FBgn0030674
• Length: 5151
• Species: Drosophila melanogaster
• Sequence 2: NP_593926.1
• Gene: pub2 / 2542432
• PomBaseID: SPAC1805.15C
• Length: 671
• Species: Schizosaccharomyces pombe
• Alignment Length: 769
• Identity: 214/769 - (27%)
• Similarity: 331/769 - (43%)
• Gaps: 169/769 - (21%)

  Fly  4425 SLLPNLAVHEGIMQDRFTAEHVIISAPKNAKPTCELQLPSMKKLMSNSSAQPYFLRTLKIFMQVR 4489
            ||.|.|.:  .:..|:|...:|       |..|..|.....:||    :..|..:..|::|
pombe    26 SLKPYLLI--SVDDDQFIKTNV-------ASGTLRLSWGFTQKL----TVSPQSIILLQLF---- 73

  Fly  4490 DMYEAQNGLSTGGGVACGA---------GDNDDDI---IDIEGDGGASGGS------SSDMAPGS 4536
              .|.|...::.|.|..||         .:..||.   |.:....|:..||      .|...|..
pombe    74 --DEKQKNETSDGFVGLGAAVVNSFLPFNNPKDDYKTRITLRSPSGSYRGSVVCLFKRSKFLPEE 136

  Fly  4537 ASTISPPPPMETNQIAQDGDDD------EEDDDEARSFPGVGNH---------AVASA------- 4579
            .       |.:.:||..|..||      |...||.       .|         :|.||
pombe   137 L-------PADKSQICTDIIDDASGCAWETRIDEF-------GHVYYLKSPQLSVISAISHEKLE 187

  Fly  4580 ---SKSTKPTLSQILCLDVLWNTLSECLVALE-ESKDEFAVLVLQPTVEAFFLIHASHRGASKKS 4640
               .|..|...||.     |:|..|:..:.:. |.|      |::..:|.:.|..:..:..:.:.
pombe   188 NLTPKQLKEVFSQF-----LFNNQSKSSLKINLEYK------VIKHLLEHYPLALSVRQQVAVEK 241

  Fly  4641 GRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTN 4705
            |    |..||:.:|.                :::|..:..|.|:..|..|     :||
pombe   242 G----PLPAGWEMRL----------------SEDYHVYFVDHSTKTTTWS-----DPR------- 274

  Fly  4706 TPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILD 4770
                .|...|.::|.:...::|               :|...::...::.|.|...:.|
pombe   275 ----DNVVASDSVSENTDSIQQ---------------INDEYQRKIAYMYDRPEMAVND------ 314

  Fly  4771 FDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDA 4835
                     .:|:            :.|.|.|.|||::.::.:|...:.|.:..|.|.:|:|.|.
pombe   315 ---------AQLQ------------LKVSRATTFEDAYDIISKLSVSDMKKKLLIRFRNEDGLDY 358

  Fly  4836 GGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKL 4900
            ||:.||::.|:|..||||.|:||..:..|.....|:|.|..||:..|||:|||||:..|::..:.
pombe   359 GGVSREFFYILSHAIFNPGYSLFEYATDDNYGLQISPLSSVNPDFRSYFRFVGRVMGLAIYHRRY 423

  Fly  4901 LECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRD 4965
            |:..|...|||.||.|.:...|::..|..:|:.|.::..||:.. ...|.||.|...||.:...|
pombe   424 LDVQFVLPFYKRILQKPLCLEDVKDVDEVYYESLKWIKNNDVDE-SLCLNFSVEENRFGESVTVD 487

  Fly  4966 LKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISG 5030
            |.||||:.||..:||..|::.:.:.|:..|..:|.:|...|..::||..::.||||.||:.|::|
pombe   488 LIPNGRNIAVNNQNKMNYLKALTEHKLVTSTEEQFNALKGGLNELIPDSVLQIFNENELDTLLNG 552

  Fly  5031 LPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMN 5095
            ..|||::|.|..|:|..||.....:.|||..|..:....:||.|||.||||::||.||..:.|.:
pombe   553 KRDIDVQDWKRFTDYRSYTETDDIVIWFWELLSEWSPEKKAKLLQFATGTSRLPLSGFKDMHGSD 617

  Fly  5096 GIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFG 5149
            |.:||.|.:... ..:||.||||||:||:|.|.|.::|...|..||.| :.|||
pombe   618 GPRKFTIEKVGH-ISQLPKAHTCFNRLDIPPYNSKEELEQKLTIAIQE-TAGFG 669

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
HUWE1	NP_001285288.1	DUF908	90..326	CDD:428721
		DUF913	621..989	CDD:461803
		UBA_like_SF	1467..1506	CDD:473871
		WWE	1756..1828	CDD:128922
		DUF5585	3288..3672	CDD:465521
		UBM	3670..3700	CDD:464159
		UBM	3719..3746	CDD:464159
		HECTc	4796..5149	CDD:238033	137/352 (39%)
pub2	NP_593926.1	HUL4	1..671	CDD:227354	214/769 (28%)