Protein Alignment Rab3-GEF and aex-3

DIOPT Version :10

• Sequence 1: NP_001259560.1
• Gene: Rab3-GEF / 32442
• FlyBaseID: FBgn0030613
• Length: 2154
• Species: Drosophila melanogaster
• Sequence 2: NP_741710.1
• Gene: aex-3 / 180420
• WormBaseID: WBGene00000086
• Length: 1409
• Species: Caenorhabditis elegans
• Alignment Length: 1838
• Identity: 615/1838 - (33%)
• Similarity: 885/1838 - (48%)
• Gaps: 474/1838 - (25%)

  Fly     1 MSDQQKASLCPRLVDYMAIVGA--HTTPPMPKGLQGLKAPPVQVPDLLRRYPPSDHADFPLPLDM 63
            |:|::| .:||||:|::.:||.  .|...............|..|::|||||..||.||.||.|:
 Worm     1 MNDKEK-EICPRLIDFLVVVGKRNRTRGASQSSPDATTDTTVTYPEILRRYPTDDHKDFILPTDV 64

  Fly    64 VYFCQPEGCTSVGPRRTGSAIRDMTSFVFALTDKDSGKTRYGICVNFYRPIERPSSAAGSAGAGN 128
            ..||||||||:...|...:|..|...|||.||:|||.|.|||||:|||:..:|.|:
 Worm    65 TVFCQPEGCTTTSARLRKNARNDPQFFVFMLTEKDSAKVRYGICLNFYQSFDRRST--------- 120

  Fly   129 DRPGNGGPGGHGGGAGGGAGGGGRGGRRSSAFRRESWRKSMERSSDSAFSSDYRSNVAPSDSDRE 193
                                                                      |.|..::
 Worm   121 ----------------------------------------------------------PKDEIKK 127

  Fly   194 LTSRRDSDQQRLHSHHSHHQPHHPSASPAVPKLGLMAPSADSESGGSHSPSPRASRKRTKLRNQS 258
            :..         .:||.....|                                         .|
 Worm   128 VPD---------DAHHKKRDSH-----------------------------------------VS 142

  Fly   259 LTSLCIISHHPFFTTFRECLFILKKLIDACNESSSPKRVGASQKINRDNVWTVLTGHVSDATPSI 323
            |||||.||||||.:.|.:.|.:||::||:.|..:: :|.|.     :|.||.:||||.::.....
 Worm   143 LTSLCFISHHPFVSIFHQVLLLLKRIIDSSNHRAA-QRTGL-----KDVVWAILTGHYNEPIVPE 201

  Fly   324 VLHDVREIETWILRLLSTPVPVPGSTRVEVEVLSPTVHEPLLFALPDHTRFSLVDFPLHLPLELL 388
            |:.:::|||||||.|||:||||||.|:|::||:...:.:...|||||||||:|:|||||:|.|:|
 Worm   202 VMKEIKEIETWILMLLSSPVPVPGKTKVQIEVMPMDLSQVFEFALPDHTRFTLIDFPLHIPFEIL 266

  Fly   389 GVETCLKVWTLIMQENKVLFQSRDYNALSMSVMAFVTMLYPLEYMFPVIPLLPTCLSCAEQLLLA 453
            |::..|:|.|..|.|.|::.|||:|||:||.:::.|.:|||||||||||||||..:..|||||||
 Worm   267 GIDMALRVLTAAMLEFKIVIQSRNYNAVSMCILSIVALLYPLEYMFPVIPLLPAYMPSAEQLLLA 331

  Fly   454 PTPFVIGVPASFLVYKKNFRLPDDIWVVDLDSTKLTPPTGGYEEIPPLPEPEGTILKNHLKQAML 518
            ||||:||||:||..::|...||.|:.:||||:..|..|...|  ||.||||:.|.||..||.|
 Worm   332 PTPFLIGVPSSFFHHRKIRELPSDVILVDLDTNCLQVPDDLY--IPDLPEPDATHLKERLKNA-- 392

  Fly   519 LMDEAGLGALTSMTATNTAVSSQQLLPSVRDSLQEPPLLGVSQVRLPLQTPPHSAQASQRNSMSA 583
                  :..:|:||..|..        ||.|:
 Worm   393 ------INKMTTMTVDNET--------SVTDA--------------------------------- 410

  Fly   584 QGTISSRQPSPMNSPALNPFVYGTDVDSVDVATRVAMVRFFNAQNTLANFAEHTRTLRLYPRPVV 648
                                .:|.|:||||||.|||||:|||:.|...||:||||||||||||||
 Worm   411 --------------------DFGIDIDSVDVACRVAMVQFFNSANVFGNFSEHTRTLRLYPRPVV 455

  Fly   649 AFQINSFLRSRPRASQFLNQFARTQAVEFLAEWSLTPTNVAFLRVQTGVMDPMQVGDKPKWFAHA 713
            :.|.:|||||||:.:|.:....||||||:.||..|.|.|..|:|||.|:....|||||||||:.:
 Worm   456 SLQTDSFLRSRPQCTQLITDLCRTQAVEYFAECCLCPKNETFVRVQAGIESAEQVGDKPKWFSES 520

  Fly   714 LTPIRFSVWDDGSSLNGALRSLKQLECQPTDESGSDSEGADSSSSSYSSLSDFVSEMASSDLSPS 778
            |.|:.|.|:.:.|:|:.|:|...    ...|....:.:.|.|:.:| :|:.|.|.:      ...
 Worm   521 LMPVHFMVYPNNSTLDSAIRVYN----AEIDNDDYEDDSATSTENS-NSIDDLVFD------ENQ 574

  Fly   779 LHDVFGSYNRPHVVPQTLSSNLDPALVYHPPSKLQYPEGIADAVASKEEEDEERADSPVSSSSSR 843
            :.|..|...:|..         :...:|..|..|:.|:  :::|.|        .||.:||..|.
 Worm   575 VTDAGGEVTKPLA---------EVNYIYKEPMTLELPQ--SESVVS--------IDSSLSSGRSS 620

  Fly   844 SDLSSPSFNRDSEFDFQPKGGQTLGSTTVGSGAAAPSFELATPLAMRLEATIKMASIEQESDTVS 908
            .|.|..:...|||.||.                     .||..||:
 Worm   621 PDSSLSTSAVDSEADFA---------------------RLADNLAL------------------- 645

  Fly   909 TATGKTIAAGSKLQRHPSDSESRPEKKIPPPLTPPVKQPGVSNILARTGSSGSSSSSPGRQSSQS 973
                |:.:.|:....|.||||..         :.||.|            ...:..:||..:|.:
 Worm   646 ----KSNSQGAFSFDHGSDSEYE---------STPVSQ------------RRKTIHNPGSDASDT 685

  Fly   974 SLFENFASHAKELVRETTRQSSQEGLLAHVDKFTLHAKKAAGEASKQALEVSKQAAGVSKNTLED 1038
                           .|:|.|.:.|
 Worm   686 ---------------PTSRGSIKSG---------------------------------------- 695

  Fly  1039 LTYVGKSTLGDLTKTAKEAATKKGIIKIEEHSAGGAGPPPKSPGSQLATHKQVQQSGGQGGGNNF 1103
            |...|.:||.|                                            ||.:..|.:.
 Worm   696 LRMKGLTTLTD--------------------------------------------SGEKVLGPSL 716

  Fly  1104 FSAIGTDFNGLASSTSTMFSGMFGKKSQQKQVPVQQKQPNVSAGKAKSGINFDPFPGRKGLVERT 1168
            .:||    ||.|..:.::||.:..|.:           |...|.|.::         .|.|..|.
 Worm   717 MNAI----NGYAEKSQSVFSQVINKTA-----------PKAQALKERT---------MKPLANRI 757

  Fly  1169 PLIKHSGPRQTQEELTRQQNQERSHSNAENQTFLKDVTNQVLAGEGVGWLKLNRFKKLMEDESYR 1233
            ...:|....:||...|.||.   ::..::||..:|:..:|.|.|:.||.....:.|:||||||.|
 Worm   758 EQSQHIVRSKTQPNPTSQQT---ANQQSKNQQTVKEFCDQALVGQSVGMFSAPKLKRLMEDESLR 819

  Fly  1234 TLVLSKLNKTLDKKIAPDDHIDDVCVTKPVWKGMLKCIQAIAGGLDVTFANFGLGGMASVFQLME 1298
            .||.||||..|:.|::.|:::.:|.:||..:|..:|.::|...|::|:|...|..|.||||.::|
 Worm   820 ELVCSKLNLGLEVKLSEDEYVKEVQLTKGQFKAYVKILKACLEGIEVSFNTPGCCGFASVFHVLE 884

  Fly  1299 VAHTHYWS--------------KEINEGSDMSSSLLSSHAASPMGSRENLRSPSSPNGSH----S 1345
            :||||||:              ..:...|:.|:.:|.........|..:||:|:.|.|.:    |
 Worm   885 IAHTHYWAMGGGEVITPSSSAPSTMTTPSEHSNDILKESRPKLPASTIDLRTPTKPLGQNVTPTS 949

  Fly  1346 ALGSEWAS------------PQES----RKSSTQLAHGGPGGSHSGAPINRRLSSADSQDGQSTT 1394
            ....|.|.            |:|:    :::...|  |.|.....||.....|.........:..
 Worm   950 TNNHEIAQSTRSPALPPPVPPREAPPIPKRNPPPL--GAPPKVPEGARAPPPLPPRPKVKTTAVD 1012

  Fly  1395 EMFKDMLSQKRSALKNMLTSF--DSDTTTSKDSKKSSGNLWSGKSTLSAGFRYTGGHLINTSSSP 1457
            |..::::...:.|..:. .||  |:|..|....|.:.      .:||..|.:.....|      |
 Worm  1013 ETPQNLVPNNQPAQPSS-PSFLADADEQTKPLLKPAP------PTTLPVGKQEPCKVL------P 1064

  Fly  1458 SPDSP-RVYLFEGLLGKDRLNLWNQMQFWEDAFLDAVSQERDMIGMDQGPIEMMERYKSLSESER 1521
            :|:.| |.|:::.|:...:..:|..:||||:||:|.|:|||:::||||.|.||::||.:|::||:
 Worm  1065 TPNEPVRHYIYQELILAVQHQIWQNLQFWENAFVDLVAQEREIVGMDQEPSEMIDRYSALNDSEK 1129

  Fly  1522 KRLEHDEDRLLSTMLYNLTAILVMLNVAKDEIRRKIRRLLGKSHIGLVYSQEVHNVVDQINNLNG 1586
            ||||.:|||||||:|:|:||.::|....:..:::|:||||||:|||||.|:|::.::|::.:..|
 Worm  1130 KRLELEEDRLLSTLLHNMTAYMIMCGTGQKALQQKVRRLLGKAHIGLVCSKEINKLLDELPSTQG 1194

  Fly  1587 NDIDLKPLGSRLLHRQSFTVHQGTDVNGPLRFMEVRDDGLVLRSVDGTIVERWWYERLVNMTYSP 1651
            |.|.||||||||:.:|||||..|...:|.:.||||.||.:||||:.|...||||||||||:||||
 Worm  1195 NFIPLKPLGSRLVQKQSFTVCPGQSSDGQMMFMEVCDDAVVLRSITGAATERWWYERLVNITYSP 1259

  Fly  1652 KTKLLCLWRRNGGQTQLHKYYTRKCKELYNCIKEAMERGGT--PTNVP--ELGGEFPVQDMNTGE 1712
            |||:||||||:..:..:||::|:||:|||.|:|.||||...  ..||.  .|||||||.|..|.:
 Worm  1260 KTKILCLWRRHDDKVHMHKFHTKKCRELYQCMKAAMERAAARGKVNVEGRALGGEFPVHDTETNQ 1324

  Fly  1713 GGLLQVCLEGVGLLFSNSKFFVRLDHIRKCFTQKGGIFVLEEYNPKTRNLIQRKYQSSMSDQICY 1777
            ||||||..:||.::|::::.|:.|.:|:||.|..|.:|:|||::.|...:|||:|.|.|:|||||
 Worm  1325 GGLLQVRCDGVAVIFAHNQIFIGLSNIKKCNTFGGNVFLLEEFDRKKGEIIQRRYFSQMADQICY 1389

  Fly  1778 SVLCVFSYVAAGQDQKKN 1795
            :||||||..|||..::::
 Worm  1390 AVLCVFSLAAAGHKKEEH 1407

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rab3-GEF	NP_001259560.1	uDENN	10..111	CDD:214824	48/102 (47%)
		DENN	260..486	CDD:214823	121/225 (54%)
		dDENN	613..681	CDD:129037	44/67 (66%)
aex-3	NP_741710.1	uDENN	9..112	CDD:214824	48/102 (47%)
		DENN	144..364	CDD:214823	121/225 (54%)
		dDENN	420..489	CDD:129037	45/68 (66%)