DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNAlig4 and DNL4

DIOPT Version :9

Sequence 1:	NP_572907.1	Gene:	DNAlig4 / 32322	FlyBaseID:	FBgn0030506	Length:	918	Species:	Drosophila melanogaster
Sequence 2:	NP_014647.1	Gene:	DNL4 / 854166	SGDID:	S000005531	Length:	944	Species:	Saccharomyces cerevisiae

Alignment Length:	1039	Identity:	247/1039 - (23%)
Similarity:	408/1039 - (39%)	Gaps:	237/1039 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 DIASTIKFRDIC-SLFEKIKATQ----KVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGA 63
            :.|.:..|:.:| .||.||...|    ....|....| |||......|.:|:..|.|
Yeast    13 NFAPSPDFKWLCEELFVKIHEVQINGTAGTGKSRSFK-YYEIISNFVEMWRKTVGNN-------- 68

  Fly    64 SSFYSVLRLLLPGADTGRDTYGLQITALGRLYIRVLQLPTDSSDAIRLQH------RNGNMYRDY 122
              .|..|.|.||..|  |..|.::...|.|.....|:||.:|:...||:.      :.||:
Yeast    69 --IYPALVLALPYRD--RRIYNIKDYVLIRTICSYLKLPKNSATEQRLKDWKQRVGKGGNL---- 125

  Fly   123 GDVVYSVLKPRCFNPPSN-LRLKEIHQMLDTIANE--------DTEVKQQQLIRFTEQASPEEQK 178
            ..::...:..|...|.|. :.:..::..||:::.:        .:.||.:..:...|..|..|.|
Yeast   126 SSLLVEEIAKRRAEPSSKAITIDNVNHYLDSLSGDRFASGRGFKSLVKSKPFLHCVENMSFVELK 190

  Fly   179 WLIRLLLKSLGLGIGEQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKF 243
            :...::||:..:|..|.|:....||.|||.....|||..|.:.|.|....|     ||...::|.
Yeast   191 YFFDIVLKNRVIGGQEHKLLNCWHPDAQDYLSVISDLKVVTSKLYDPKVRL-----KDDDLSIKV 250

  Fly   244 -----------VNLN--SVIRPFHQIRPMLCERFPGDIQELMQSDVLYLETKMDGERFQLH-IDR 294
                       |||:  .:.|..|                    |...:|.||||||.|:| ::.
Yeast   251 GFAFAPQLAKKVNLSYEKICRTLH--------------------DDFLVEEKMDGERIQVHYMNY 295

  Fly   295 GR-FMYISRNGVDYTRNFGHSYDHGTLTPKLRGLLPLGLESIILDGEMMVWDTNK---LRF-REK 354
            |. ..:.||.|:|||..:|.|...||::..||  ....::..:|||||:.:|..:   |.| ..|
Yeast   296 GESIKFFSRRGIDYTYLYGASLSSGTISQHLR--FTDSVKECVLDGEMVTFDAKRRVILPFGLVK 358

  Fly   355 GENTDVKSLKPEGS--WQPCFVVYDLLYFNGQSLLDHTYIQRAYKLQKLIVEQSGVLQLMRARKI 417
            |...:..|.....:  :.|.::|:||||.||.||......||...|..::.....:::::|:.:.
Yeast   359 GSAKEALSFNSINNVDFHPLYMVFDLLYLNGTSLTPLPLHQRKQYLNSILSPLKNIVEIVRSSRC 423

  Fly   418 GSVQEFNELFQQALDSHAEGIVLKKQGSRYQPGVRLGGGWYKDKADYIKGLITEFDVLIIGAFYN 482
            ..|:...:..:.|:...:||:|||...|.|....| ...|.|.|.:|::......|:::||....
Yeast   424 YGVESIKKSLEVAISLGSEGVVLKYYNSSYNVASR-NNNWIKVKPEYLEEFGENLDLIVIGRDSG 487

  Fly   483 RKRTFVDSFLLGVL----------------------QPAPPGSSNR--PEVFSIGVVANNTKQRG 523
            :|    |||:||:|                      |.....:|.|  .::.|...:||...|..
Yeast   488 KK----DSFMLGLLVLDEEEYKKHQGDSSEIVDHSSQEKHIQNSRRRVKKILSFCSIANGISQEE 548

  Fly   524 V--LNHTLKPHWHDVVNEPPP---LWFHYKPKERSGCPDLWIEPQNSVILQVKAADLAPNGA--- 580
            .  ::...:.||.......||   |.|..|      .|..||:|..|::|::|:..|.....
Yeast   549 FKEIDRKTRGHWKRTSEVAPPASILEFGSK------IPAEWIDPSESIVLEIKSRSLDNTETNMQ 607

  Fly   581 -FFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCGGPLAIKKLNKRQLRLEDVTTKRKQMRMTP 644
             :.|..:|:....:..|.||.|::|.||   |||                     .:.:.::..|
Yeast   608 KYATNCTLYGGYCKRIRYDKEWTDCYTL---NDL---------------------YESRTVKSNP 648

  Fly   645 S---ERSRLGLAVYEKRYDASTSAS---------TSKLFDGLSFCILS-----GSAGRHSKHQLQ 692
            |   |||:||| :.:||.....|.|         .|.:|.||.|.:||     .:..|.::.:|:
Yeast   649 SYQAERSQLGL-IRKKRKRVLISDSFHQNRKQLPISNIFAGLLFYVLSDYVTEDTGIRITRAELE 712

  Fly   693 ELAVKNGGCIVENPL-----PNDPKCFCIAGDETFLVKRLILQQPRTCDIVRMEWLLR-VCQKQE 751
            :..|::||.::.|.:     ..|.:  .|:...|...|.||   .|..||:...|:|. :..|:.
Yeast   713 KTIVEHGGKLIYNVILKRHSIGDVR--LISCKTTTECKALI---DRGYDILHPNWVLDCIAYKRL 772

  Fly   752 LELKPRDLIAATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGIELTADNVAGITASNLNAL 816
            :.::|......::.::....:..|..|||:..||:.. :|..|.:         :.::...:..|
Yeast   773 ILIEPNYCFNVSQKMRAVAEKRVDCLGDSFENDISET-KLSSLYK---------SQLSLPPMGEL 827

  Fly   817 EDQLLDGK-KNLNMFRNLNAFFYSPH-----GDEVAKLLFLQNGGRIVDD-----------SDPQ 864
            |   :|.: :...:|...|...|.|.     .|::.::.....||:|.|.           :||.
Yeast   828 E---IDSEVRRFPLFLFSNRIAYVPRRKISTEDDIIEMKIKLFGGKITDQQSLCNLIIIPYTDPI 889

  Fly   865 LNLGFICMSSDIDNDHFEHWLHNHSKLTTD-----------KVLNSAWIHQCHREGILLPMHSF 917
            |...  ||:            ..|.|:...           :|:...|:.....|...:|...|
Yeast   890 LRKD--CMN------------EVHEKIKEQIKASDTIPKIARVVAPEWVDHSINENCQVPEEDF 939

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNAlig4	NP_572907.1	DNA_ligase_A_N	10..197	CDD:282522	49/206 (24%)
		dnl1	84..613	CDD:273147	151/597 (25%)
		Adenylation_DNA_ligase_IV	249..466	CDD:185713	64/224 (29%)
		OBF_DNA_ligase_IV	469..607	CDD:153437	39/170 (23%)
		BRCT	668..745	CDD:214602	23/86 (27%)
DNL4	NP_014647.1	DNA_ligase_A_N	20..207	CDD:282522	48/203 (24%)
		dnl1	84..717	CDD:273147	174/699 (25%)
		Adenylation_DNA_ligase_IV	244..471	CDD:185713	68/249 (27%)
		OBF_DNA_ligase_IV	474..635	CDD:153437	39/170 (23%)
		BRCT	681..767	CDD:278934	24/90 (27%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346790
Domainoid	1	1.000	86	1.000	Domainoid score	I1881
eggNOG	1	0.900	-	-		E1_COG1793
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H1736
Inparanoid	1	1.050	168	1.000	Inparanoid score	I1023
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54896
OrthoFinder	1	1.000	-	-		FOG0004767
OrthoInspector	1	1.000	-	-		oto99807
orthoMCL	1	0.900	-	-		OOG6_103257
Panther	1	1.100	-	-	LDO	PTHR45997
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R326
SonicParanoid	1	1.000	-	-		X3920
TreeFam	1	0.960	-	-
	15	14.790