Protein Alignment DNAlig4 and DNL4

DIOPT Version :10

• Sequence 1: NP_572907.1
• Gene: DNAlig4 / 32322
• FlyBaseID: FBgn0030506
• Length: 918
• Species: Drosophila melanogaster
• Sequence 2: NP_014647.1
• Gene: DNL4 / 854166
• SGDID: S000005531
• Length: 944
• Species: Saccharomyces cerevisiae
• Alignment Length: 1039
• Identity: 247/1039 - (23%)
• Similarity: 408/1039 - (39%)
• Gaps: 237/1039 - (22%)

  Fly     4 DIASTIKFRDIC-SLFEKIKATQ----KVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGA 63
            :.|.:..|:.:| .||.||...|    ....|....| |||......|.:|:..|.|
Yeast    13 NFAPSPDFKWLCEELFVKIHEVQINGTAGTGKSRSFK-YYEIISNFVEMWRKTVGNN-------- 68

  Fly    64 SSFYSVLRLLLPGADTGRDTYGLQITALGRLYIRVLQLPTDSSDAIRLQH------RNGNMYRDY 122
              .|..|.|.||..|  |..|.::...|.|.....|:||.:|:...||:.      :.||:
Yeast    69 --IYPALVLALPYRD--RRIYNIKDYVLIRTICSYLKLPKNSATEQRLKDWKQRVGKGGNL---- 125

  Fly   123 GDVVYSVLKPRCFNPPSN-LRLKEIHQMLDTIANE--------DTEVKQQQLIRFTEQASPEEQK 178
            ..::...:..|...|.|. :.:..::..||:::.:        .:.||.:..:...|..|..|.|
Yeast   126 SSLLVEEIAKRRAEPSSKAITIDNVNHYLDSLSGDRFASGRGFKSLVKSKPFLHCVENMSFVELK 190

  Fly   179 WLIRLLLKSLGLGIGEQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKF 243
            :...::||:..:|..|.|:....||.|||.....|||..|.:.|.|....|     ||...::|.
Yeast   191 YFFDIVLKNRVIGGQEHKLLNCWHPDAQDYLSVISDLKVVTSKLYDPKVRL-----KDDDLSIKV 250

  Fly   244 -----------VNLN--SVIRPFHQIRPMLCERFPGDIQELMQSDVLYLETKMDGERFQLH-IDR 294
                       |||:  .:.|..|                    |...:|.||||||.|:| ::.
Yeast   251 GFAFAPQLAKKVNLSYEKICRTLH--------------------DDFLVEEKMDGERIQVHYMNY 295

  Fly   295 GR-FMYISRNGVDYTRNFGHSYDHGTLTPKLRGLLPLGLESIILDGEMMVWDTNK---LRF-REK 354
            |. ..:.||.|:|||..:|.|...||::..||  ....::..:|||||:.:|..:   |.| ..|
Yeast   296 GESIKFFSRRGIDYTYLYGASLSSGTISQHLR--FTDSVKECVLDGEMVTFDAKRRVILPFGLVK 358

  Fly   355 GENTDVKSLKPEGS--WQPCFVVYDLLYFNGQSLLDHTYIQRAYKLQKLIVEQSGVLQLMRARKI 417
            |...:..|.....:  :.|.::|:||||.||.||......||...|..::.....:::::|:.:.
Yeast   359 GSAKEALSFNSINNVDFHPLYMVFDLLYLNGTSLTPLPLHQRKQYLNSILSPLKNIVEIVRSSRC 423

  Fly   418 GSVQEFNELFQQALDSHAEGIVLKKQGSRYQPGVRLGGGWYKDKADYIKGLITEFDVLIIGAFYN 482
            ..|:...:..:.|:...:||:|||...|.|....| ...|.|.|.:|::......|:::||....
Yeast   424 YGVESIKKSLEVAISLGSEGVVLKYYNSSYNVASR-NNNWIKVKPEYLEEFGENLDLIVIGRDSG 487

  Fly   483 RKRTFVDSFLLGVL----------------------QPAPPGSSNR--PEVFSIGVVANNTKQRG 523
            :|    |||:||:|                      |.....:|.|  .::.|...:||...|..
Yeast   488 KK----DSFMLGLLVLDEEEYKKHQGDSSEIVDHSSQEKHIQNSRRRVKKILSFCSIANGISQEE 548

  Fly   524 V--LNHTLKPHWHDVVNEPPP---LWFHYKPKERSGCPDLWIEPQNSVILQVKAADLAPNGA--- 580
            .  ::...:.||.......||   |.|..|      .|..||:|..|::|::|:..|.....
Yeast   549 FKEIDRKTRGHWKRTSEVAPPASILEFGSK------IPAEWIDPSESIVLEIKSRSLDNTETNMQ 607

  Fly   581 -FFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCGGPLAIKKLNKRQLRLEDVTTKRKQMRMTP 644
             :.|..:|:....:..|.||.|::|.||   |||                     .:.:.::..|
Yeast   608 KYATNCTLYGGYCKRIRYDKEWTDCYTL---NDL---------------------YESRTVKSNP 648

  Fly   645 S---ERSRLGLAVYEKRYDASTSAS---------TSKLFDGLSFCILS-----GSAGRHSKHQLQ 692
            |   |||:||| :.:||.....|.|         .|.:|.||.|.:||     .:..|.::.:|:
Yeast   649 SYQAERSQLGL-IRKKRKRVLISDSFHQNRKQLPISNIFAGLLFYVLSDYVTEDTGIRITRAELE 712

  Fly   693 ELAVKNGGCIVENPL-----PNDPKCFCIAGDETFLVKRLILQQPRTCDIVRMEWLLR-VCQKQE 751
            :..|::||.::.|.:     ..|.:  .|:...|...|.||   .|..||:...|:|. :..|:.
Yeast   713 KTIVEHGGKLIYNVILKRHSIGDVR--LISCKTTTECKALI---DRGYDILHPNWVLDCIAYKRL 772

  Fly   752 LELKPRDLIAATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGIELTADNVAGITASNLNAL 816
            :.::|......::.::....:..|..|||:..||:.. :|..|.:         :.::...:..|
Yeast   773 ILIEPNYCFNVSQKMRAVAEKRVDCLGDSFENDISET-KLSSLYK---------SQLSLPPMGEL 827

  Fly   817 EDQLLDGK-KNLNMFRNLNAFFYSPH-----GDEVAKLLFLQNGGRIVDD-----------SDPQ 864
            |   :|.: :...:|...|...|.|.     .|::.::.....||:|.|.           :||.
Yeast   828 E---IDSEVRRFPLFLFSNRIAYVPRRKISTEDDIIEMKIKLFGGKITDQQSLCNLIIIPYTDPI 889

  Fly   865 LNLGFICMSSDIDNDHFEHWLHNHSKLTTD-----------KVLNSAWIHQCHREGILLPMHSF 917
            |...  ||:            ..|.|:...           :|:...|:.....|...:|...|
Yeast   890 LRKD--CMN------------EVHEKIKEQIKASDTIPKIARVVAPEWVDHSINENCQVPEEDF 939

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
DNAlig4	NP_572907.1	dnl1	84..613	CDD:273147	151/597 (25%)
		Adenylation_DNA_ligase_IV	249..466	CDD:185713	64/224 (29%)
		BRCT	670..760	CDD:469589	25/100 (25%)
		BRCT_2	830..917	CDD:465186	20/113 (18%)
DNL4	NP_014647.1	dnl1	84..717	CDD:273147	174/699 (25%)
		BRCT_DNA_ligase_IV_rpt1	690..782	CDD:349354	22/96 (23%)
		BRCT_MDC1_rpt1	837..934	CDD:349375	19/110 (17%)