Protein Alignment Ndc80 and Ndc80

DIOPT Version :10

• Sequence 1: NP_572902.1
• Gene: Ndc80 / 32316
• FlyBaseID: FBgn0030500
• Length: 675
• Species: Drosophila melanogaster
• Sequence 2: NP_075783.2
• Gene: Ndc80 / 67052
• MGIID: 1914302
• Length: 642
• Species: Mus musculus
• Alignment Length: 701
• Identity: 142/701 - (20%)
• Similarity: 283/701 - (40%)
• Gaps: 166/701 - (23%)

  Fly    17 LEERGTAD---------KRQTRATKRSAATHTPSSSQAAP---RRPSASRLPQPLNLERDRASQM 69
            ::|..|.|         :.:.|:|....:||.|:|.:...   :|.|.         ...|.||:
Mouse    16 MQELRTLDLNKPGLYTPQTKERSTFGKLSTHKPTSERKVSIFGKRTSG---------HGSRNSQL 71

  Fly    70 GVTPKRGHRFVPSTAERAIAPHSDKKWVAERAQQILEYLHGIQNSEAPTGLIADLFSRPGGLRHM 134
            |:       |..|...:...|.:||.::.:..:|:.|:|       ...|.:..:..:  .|:..
Mouse    72 GI-------FSSSEKIKDPRPLNDKAFIQQCIRQLYEFL-------TENGYVYSVSMK--SLQAP 120

  Fly   135 TIKQFVSILNFMFHHIWRNRVTVGQNHVEDITSAMQKLQYPYQVNKSWLVSPTTQHSFGHVIVLL 199
            :.|:|:.|..|::..:..:....|....|::....:.|.||:.::||.:.:....|::.|::..|
Mouse   121 STKEFLKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGYPFTLSKSSMYTVGAPHTWPHIVAAL 185

  Fly   200 DFLMDFLPPLPSSDVVEEEFPFMETMEQPSSYLNSMHCESTTIMSTTQAHAIQLDEDV--NGLLF 262
            .:|:|.:.                        :::...||:.:....|....:.::.:  |.|..
Mouse   186 VWLIDCIK------------------------IDTAMKESSPLFDDGQLWGEETEDGIKHNKLFL 226

  Fly   263 EEASKCIALWDQELT------NEEAKLQAETRD--QVISKKCDL--PDRKALDQLIGDLKTKLQQ 317
            |...||   :::.:|      .|:|:|||:.:|  :|.:.|.:.  .:.|||::.|..|:.:.::
Mouse   227 EYTKKC---YEKFMTGADSFEEEDAELQAKLKDLYKVDASKLESLEAENKALNEQIARLEEERER 288

  Fly   318 LENKLHDPSEDNKLNKLERLTKEHDQLAQQLAASEEELIKKLKLYEQLSAQVEEKQSNLRQQMKY 382
                     |.|:|..|::|........|...|....|...|.:.:|.|..::|:...:.|:.:.
Mouse   289 ---------EPNRLMSLKKLKASLQADVQNYKAYMSNLESHLAVLKQKSNSLDEEIGRVEQECET 344

  Fly   383 EK----RLEQAVNRQKFSAQQLKELQMKCDDMENYSKAYERQV--KEVSELELHQQVM----LSR 437
            .|    ||:..|:.||:|...::.:        |:.|...:|.  |...:||..||.|    |..
Mouse   345 VKQENTRLQSIVDNQKYSVADIERI--------NHEKNELQQTINKLTKDLEAEQQQMWNEELKY 401

  Fly   438 AKQKQLDSVEVFNSH--VRHLSMDPVICGLIKSGIGQQS-DLTLPLNPNQED---ISERVQCLEL 496
            |:.|:....::...|  .|.|.:.|      |.....:. |..:..||....   :..|.|....
Mouse   402 ARGKEAIEAQLAEYHKLARKLKLIP------KGAENSKGYDFEIKFNPEAGANCLVKYRTQVYAP 460

  Fly   497 LGKLLQQHRQQNIDRRQMLDKQVAKVK--SDSIELDTEIATMDSQLRAQKQRLTKMEASYRTKRD 559
            |.:||.:..::       ::|.:.|.:  .|::|   ::.||.::.: ...|:.|.|.
Mouse   461 LKELLNESEEE-------INKALNKKRHLEDTLE---QLNTMKTESK-NTVRMLKEEI------- 507

  Fly   560 MQAQHRQQLLEDQYDQTARLDELEKREKQALEKLEASKQRNEDLLTAAEQFQEQDLKARQDRLEK 624
                   |.|:|.:.|..:  |.|:::|::..:|| |.::::.||.:.
Mouse   508 -------QKLDDLHQQAVK--EAEEKDKKSASELE-SLEKHKHLLESG----------------- 545

  Fly   625 CEQKLAKAEDQLLAVESKVNATQAKLNEVEQKVYSAKLPSFQPVLDAIKNH 675
            ....|::|.|:|.||:.:...|.....|..:||.:    :.|.:|:.:..|
Mouse   546 VNDGLSEAMDELDAVQREYQLTVKTTTEERRKVEN----NLQRLLEMVATH 592

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ndc80	NP_572902.1	Ndc80_HEC	48..204	CDD:461058	31/155 (20%)
		SMC_N	310..>661	CDD:481474	77/368 (21%)
Ndc80	NP_075783.2	Interaction with the N-terminus of CDCA1. /evidence=ECO:0000250|UniProtKB:O14777	1..445		95/462 (21%)
		Nuclear localization. /evidence=ECO:0000250|UniProtKB:O14777	1..250		44/245 (18%)
		Ndc80_HEC	51..206	CDD:461058	30/154 (19%)
		Interaction with RB1. /evidence=ECO:0000250|UniProtKB:O14777	128..251		20/122 (16%)
		DUF5595	213..282	CDD:465636	11/76 (14%)
		SMC_prok_B	240..>556	CDD:274008	74/345 (21%)
		Interaction with NEK2 and ZWINT. /evidence=ECO:0000250|UniProtKB:O14777	251..618		85/396 (21%)
		Interaction with SMC1A. /evidence=ECO:0000250|UniProtKB:O14777	251..431		45/197 (23%)
		Interaction with PSMC2 and SMC1A. /evidence=ECO:0000250|UniProtKB:O14777	361..547		43/203 (21%)
		Interaction with the C-terminus of CDCA1 and the SPBC24-SPBC25 subcomplex. /evidence=ECO:0000250|UniProtKB:O14777	446..642		40/203 (20%)