Protein Alignment Polr2A and RPA190

DIOPT Version :10

• Sequence 1: NP_511124.1
• Gene: Polr2A / 32100
• FlyBaseID: FBgn0003277
• Length: 1887
• Species: Drosophila melanogaster
• Sequence 2: NP_014986.3
• Gene: RPA190 / 854519
• SGDID: S000005868
• Length: 1664
• Species: Saccharomyces cerevisiae
• Alignment Length: 1823
• Identity: 473/1823 - (25%)
• Similarity: 750/1823 - (41%)
• Gaps: 506/1823 - (27%)

  Fly     1 MSTPTDSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEG-GRPKLGGLMDPRQGVIDRT 64
            :|.|..|     ::..|.||||:..|||.:|..:  :.....::. |.|..|||.|...|...|
Yeast     3 ISKPVGS-----EITSVDFGILTAKEIRNLSAKQ--ITNPTVLDNLGHPVSGGLYDLALGAFLR- 59

  Fly    65 SRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYC------------------- 110
            :.|.||..:...||||.|||:|..|.::..|..:....||..|.:|
Yeast    60 NLCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRASCLFCHHFRLKSVEVHRYACKLRL 124

  Fly   111 ---------------------SKMLVSPHNPKIKEIVMKSRGQPR--------------KRLAYV 140
                                 |.|.......:..|..|...|..:              ||..||
Yeast   125 LQYGLIDESYKLDEITLGSLNSSMYTDDEAIEDNEDEMDGEGSKQSKDISSTLLNELKSKRSEYV 189

  Fly   141 YDLCKGKTICEGGEDMDLTKENQQ-----PDPNKKPGH------------GGCGHYQPSIRRTGL 188
             |:...|.:.:|     .|.|...     .|..||..|            ..||.:.|..|:.|.
Yeast   190 -DMAIAKALSDG-----RTTERGSFTATVNDERKKLVHEFHKKLLSRGKCDNCGMFSPKFRKDGF 248

  Fly   189 DL---TAEWKHQ---------------NEDSQEKKIVVSAERVWEILKHITDEECFILGMDP--- 232
            ..   ||..:.|               .:..|.||:..|.|      ....|||.|.:|.:|
Yeast   249 TKIFETALNEKQITNNRVKGFIRQDMIKKQKQAKKLDGSNE------ASANDEESFDVGRNPTTR 307

  Fly   233 ----------------------------------------KYARPDWMIVTVLPVPPLAVR-PAV 256
                                                    |..:.|...:.||.|||...| |:
Yeast   308 PKTGSTYILSTEVKNILDTVFRKEQCVLQYVFHSRPNLSRKLVKADSFFMDVLVVPPTRFRLPS- 371

  Fly   257 VMFGAAKNQDDLTHKLSDIIKAN----------NELRKNEASGAAAHVIQENIKMLQFHVATLVD 311
             ..|...:::.....||.::..:          ::|:|::.|.....||...:    .:....:.
Yeast   372 -KLGEEVHENSQNQLLSKVLTTSLLIRDLNDDLSKLQKDKVSLEDRRVIFSRL----MNAFVTIQ 431

  Fly   312 NDMPGMPRAMQKSGK-----PLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVG 371
            ||:.....:.:..|:     |:..:|..|:.|||..|.::|||||:::||:||:||||:..:::|
Yeast   432 NDVNAFIDSTKAQGRTSGKVPIPGVKQALEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIG 496

  Fly   372 VPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDL--------------R 422
            ||...|..||:||.||.:||..:::.|..|..::|||..|..::|..:.|              .
Yeast   497 VPPVFAVKLTYPEPVTAYNIAELRQAVINGPDKWPGATQIQNEDGSLVSLIGMSVEQRKALANQL 561

  Fly   423 FHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLP-WSTFRMNLSCTSPYNA 486
            ..|.|:........||.||:::.|:|:.||||||||.|||||:|:||| ..|.|::.:.|..|||
Yeast   562 LTPSSNVSTHTLNKKVYRHIKNRDVVLMNRQPTLHKASMMGHKVRVLPNEKTLRLHYANTGAYNA 626

  Fly   487 DFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQ 551
            ||||||||:|.||:...|||..|:..|..|.:||.:..||.|::||.::|...:|.:|.|.||||
Yeast   627 DFDGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPVRGLIQDHISAGVWLTSKDSFFTREQ 691

  Fly   552 ----------------VMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSLII--------PGNV 592
                            ..:.::.||        |.|.||.||||||||.:.::        || :
Yeast   692 YQQYIYGCIRPEDGHTTRSKIVTLP--------PTIFKPYPLWTGKQIITTVLLNVTPPDMPG-I 747

  Fly   593 NMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIA 657
            |:|..:....:...:|..      :.:|:.:.|.|:.|||.|...|.|...::|......|.::|
Yeast   748 NLISKNKIKNEYWGKGSL------ENEVLFKDGALLCGILDKSQYGASKYGIVHSLHEVYGPEVA 806

  Fly   658 GRFYGNIQTVINNWLLFEGHSIGIGD----------------TIADP--QTYNEIQQAIKKAKDD 704
            .:....:..:..|::.....:.|:.|                |..|.  :...|:....|....|
Yeast   807 AKVLSVLGRLFTNYITATAFTCGMDDLRLTAEGNKWRTDILKTSVDTGREAAAEVTNLDKDTPAD 871

  Fly   705 VINVIQKAHNMELEPTPGNTLRQTFENKVNRILNDARDK--TGGSAKKSLTEYNNLKAMVVSGSK 767
            ...::::...:..:......|.....:|||.|.:....|  ..|:.||  ...|:::||.:||:|
Yeast   872 DPELLKRLQEILRDNNKSGILDAVTSSKVNAITSQVVSKCVPDGTMKK--FPCNSMQAMALSGAK 934

  Fly   768 GSNINISQVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHA 832
            |||:|:||::..:|||.:||:|:|.....:|||.|...:....:.|:|:..:.:|:.|.|:|||.
Yeast   935 GSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPYETDAMAGGYVKGRFYSGIKPQEYYFHC 999

  Fly   833 MGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQN 897
            |.||||||||||||:.:||:||.|.|.:|.|.|:||.::|::.|.|:|..||.|
Yeast  1000 MAGREGLIDTAVKTSRSGYLQRCLTKQLEGVHVSYDNSIRDADGTLVQFMYGGD----------- 1053

  Fly   898 MPTVKLSNKSFEKRFKFDWSN-ERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQI 961
              .:.::.:|...:|:|...| ..|:||.          :.|..|:.|:.|
Yeast  1054 --AIDITKESHMTQFEFCLDNYYALLKKY----------NPSALIEHLDVE-------------- 1092

  Fly   962 FPNGESKVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQAN 1026
                                               |.::..|  |||..|       .:.||:.:
Yeast  1093 -----------------------------------SALKYSK--KTLKYR-------KKHSKEPH 1113

  Fly  1027 ENATLLFQCLIRSTLCTKY---VSEEFRLSTEAF----------------EWLVGEIETRFQQAQ 1072
            ...::.:..::......||   |||.|:...|:|                :.....::.::.::.
Yeast  1114 YKQSVKYDPVLAKYNPAKYLGSVSENFQDKLESFLDKNSKLFKSSDGVNEKKFRALMQLKYMRSL 1178

  Fly  1073 ANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEII-NISKKPKAPSLT---- 1132
            .||||.||.:|:||:|||:||||||||||||..:.|||||:|||:||: ..|...|.|.:|
Yeast  1179 INPGEAVGIIASQSVGEPSTQMTLNTFHFAGHGAANVTLGIPRLREIVMTASAAIKTPQMTLPIW 1243

  Fly  1133 --------------------------VFLT----------GGAAR-------------------- 1141
                                      |.:|          |.|||
Yeast  1244 NDVSDEQADTFCKSISKVLLSEVIDKVIVTETTGTSNTAGGNAARSYVIHMRFFDNNEYSEEYDV 1308

  Fly  1142 DAEKAKNVLCR-----LEHTTLRKV---------------------TANTAIYYDPDPQRTVISE 1180
            ..|:.:||:..     ||...::::                     .||::    .:.:|.....
Yeast  1309 SKEELQNVISNQFIHLLEAAIVKEIKKQKRTTGPDIGVAVPRLQTDVANSS----SNSKRLEEDN 1369

  Fly  1181 DQEFVN------VYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQI---------AEKINVG 1230
            |:|..:      |.|:.||.|           |::..|..:|....|.|         :|..:|.
Yeast  1370 DEEQSHKKTKQAVSYDEPDED-----------EIETMREAEKSSDEEGIDSDKESDSDSEDEDVD 1423

  Fly  1231 FGEDLN-CIFNDDNADKLVLRIR---IMNNEE--NKFQDEDEAVDKMEDDMFLRCIEANMLSDMT 1289
            ..|.:| .|...:|....|.|.|   |:::..  .|:..:||:....|..:.|......:|....
Yeast  1424 MNEQINKSIVEANNNMNKVQRDRQSAIISHHRFITKYNFDDESGKWCEFKLELAADTEKLLMVNI 1488

  Fly  1290 LQGI--EAIGKVYMHL-----PQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPI 1347
            ::.|  ::|.:...|:     |:.::.||:::||...|:|:  |    |..:.:      |||.|
Yeast  1489 VEEICRKSIIRQIPHIDRCVHPEPENGKRVLVTEGVNFQAM--W----DQEAFI------DVDGI 1541

  Fly  1348 RTSSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGIN 1412
              :|||:..:.:..|:||.|.::..|:|.|...|.:.|::|||.|:.|:||.:|..:|..|.|:
Yeast  1542 --TSNDVAAVLKTYGVEAARNTIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQGM- 1603

  Fly  1413 RQDTGALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIE 1477
            ...|.:.|:.|:|.|...|..|....|.:.:...|..|::|:|..:|||.||:|         .:
Yeast  1604 ETSTSSFMKMSYETTCQFLTKAVLDNEREQLDSPSARIVVGKLNNVGTGSFDVL---------AK 1659

  Fly  1478 IPN 1480
            :||
Yeast  1660 VPN 1662

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr2A	NP_511124.1	RNAP_II_RPB1_N	15..868	CDD:259848	295/1060 (28%)
		RNA_pol_Rpb1_6	890..1071	CDD:461511	27/200 (14%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	140/548 (26%)
		Herpes_BLLF1	<1498..1862	CDD:282904
		PTZ00449	<1707..1880	CDD:185628
RPA190	NP_014986.3	RNAP_I_RPA1_N	16..1035	CDD:259844	294/1056 (28%)
		RNA_pol_Rpb1_5	989..1611	CDD:398596	184/732 (25%)