Protein Alignment Polr2A and RPO21

DIOPT Version :10

• Sequence 1: NP_511124.1
• Gene: Polr2A / 32100
• FlyBaseID: FBgn0003277
• Length: 1887
• Species: Drosophila melanogaster
• Sequence 2: NP_010141.1
• Gene: RPO21 / 851415
• SGDID: S000002299
• Length: 1733
• Species: Saccharomyces cerevisiae
• Alignment Length: 1913
• Identity: 947/1913 - (49%)
• Similarity: 1250/1913 - (65%)
• Gaps: 222/1913 - (11%)

  Fly     7 SKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETME--GGRPKLGGLMDPRQGVIDRTSRCQT 69
            |.||||.||.||||:.||:|:|.:||.:  ::|.|||:  ..|.|:|||.|||.|.|||..:|||
Yeast     7 SSAPLRTVKEVQFGLFSPEEVRAISVAK--IRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQT 69

  Fly    70 CAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKE-IVMKSRGQP 133
            |...|.|||||||||||||||||:|||.|..|:..|||.:|.|:|:..||..::: :.:|   ..
Yeast    70 CQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIK---DS 131

  Fly   134 RKRLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQN 198
            :||.|.::.|||.|.:||    .|:..|:   ||.:....||||:.||:||:.||.|...||...
Yeast   132 KKRFAAIWTLCKTKMVCE----TDVPSED---DPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDR 189

  Fly   199 E--DSQEKKI-VVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFG 260
            .  |:.|.:: |:|.|.:..|.|||:.::...||.:..::||:|||:|.|||||..|||::....
Yeast   190 ATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNE 254

  Fly   261 AAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSG 325
            :.:.:||||.||:||:|||..|...|.:||..|.|:|...:|||||||.:|||:.|.|:|:||||
Yeast   255 SQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSG 319

  Fly   326 KPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFN 390
            :|:|:|:|||||||||||||||||||||||||||:.||||.:||||||:|||:.||:||:|||:|
Yeast   320 RPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYN 384

  Fly   391 IDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPT 455
            |||:.:|||.|.:::|||||::||:|:|||||:..::.|:.||.|:|||||:.|:|.|:|||||:
Yeast   385 IDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPS 449

  Fly   456 LHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITP 520
            |||||||.|||||:|:||||:|||.||||||||||||||||||||.|||||:..:...|.||::|
Yeast   450 LHKMSMMAHRVKVIPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSP 514

  Fly   521 QANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFS 585
            |:|||.||||||||..:||:|.||.||..:||:|:|.::|.||..:|.|.|:||:|||:||||.|
Yeast   515 QSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILS 579

  Fly   586 LIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFL 650
            :.||..:::.|.        |||. ..:||.|..:::..|::|.|::.||::|:|.|.|:|:...
Yeast   580 VAIPNGIHLQRF--------DEGT-TLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTR 635

  Fly   651 ELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNM 715
            |.|..:..:.:||||.|:|.|||..|.|.||||||||..|..||.:.|.:||..|::|.::|...
Yeast   636 EKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQAN 700

  Fly   716 ELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACV 780
            .|....|.|||::||:.|.|.||:||||.|..|:.:|.:.||:|.||::|||||.|||:|:.|||
Yeast   701 LLTAKHGMTLRESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACV 765

  Fly   781 GQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVK 845
            |||:||||||.:||..||||||.||||.|||:||||||||.||||.||:||||||||||||||||
Yeast   766 GQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVK 830

  Fly   846 TAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEK 910
            |||||||||||:||:|.:||:||.|.|||:|.:||..|||||:....:|.|::.|:..|:.:|||
Yeast   831 TAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEK 895

  Fly   911 RFKFDWSNERLMKKVFTDDVIKE--MTDSSEAIQE------LEAEWDRLVSDRDSLRQIFPNGES 967
            |::.|..|        ||..:..  :...||.:.:      |:.|:.:||.||..||::|.:||:
Yeast   896 RYRVDLLN--------TDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEA 952

  Fly   968 KVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLL 1032
            ...||.|::|:|.|.|:.|||:...|:||:...::.|||.|.|..:::.|.:.|.:.|..:|..|
Yeast   953 NWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTL 1017

  Fly  1033 FQCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLN 1097
            |.||:||.|.|:.|.:|:||:.:||:|::..||.:|.::..:||||||.|||||:||||||||||
Yeast  1018 FCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMTLN 1082

  Fly  1098 TFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVT 1162
            |||||||:||.||.||||||||:|::|..|.|||||:|..|.|.|.|:||.:...:|||||:.||
Yeast  1083 TFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEHTTLKSVT 1147

  Fly  1163 ANTAIYYDPDPQRTVISEDQEFVNVYYEMPD------FDPTRISPWLLRIELDRKRMTDKKLTME 1221
            ..:.|||||||:.|||.||:|.:.:::.:.|      ||  :.||||||:||||..|.||.|||.
Yeast  1148 IASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFD--QQSPWLLRLELDRAAMNDKDLTMG 1210

  Fly  1222 QIAEKINVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLS 1286
            |:.|:|...|..||..|:::||.:||::|.|::  .......|.||    |:|..|:.||..||.
Yeast  1211 QVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVV--RPKSLDAETEA----EEDHMLKKIENTMLE 1269

  Fly  1287 DMTLQGIEAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSS 1351
            ::||:|:|.|.:|.|  .:.|.|   |.:.|||:....||:|||||.::.:|::...:||.|..:
Yeast  1270 NITLRGVENIERVVM--MKYDRK---VPSPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYT 1329

  Fly  1352 NDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDT 1416
            |...:|.:||||||.|.::.||:..|:...|.||||||:|||.||||.:|.|.::||||.||.:|
Yeast  1330 NSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNT 1394

  Fly  1417 GALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIPNT 1481
            ||||||||||||::|.:|.|.||.|..||||||:|:||:..:|||.||:::|.|          :
Yeast  1395 GALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGAFDVMIDEE----------S 1449

  Fly  1482 LGNSMLGGAAMFIGGGSTPSMTPPMTPWANCNTPRYFSPPGHVSA--------MTPGGPSFSP-- 1536
            |...|.......|..|....:||            |.:..|.|:|        |      |||
Yeast  1450 LVKYMPEQKITEIEDGQDGGVTP------------YSNESGLVNADLDVKDELM------FSPLV 1496

  Fly  1537 -SAASDA-SGMSPSWSPAHPGSSPSSPGPSMSPYFPASPSVSPSYSPTSPNYTASSPGGASPNYS 1599
             |.::|| :|...::..|..|.:             .||..:...:||||.:..||||     :|
Yeast  1497 DSGSNDAMAGGFTAYGGADYGEA-------------TSPFGAYGEAPTSPGFGVSSPG-----FS 1543

  Fly  1600 PSSPNYSPTSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNI 1664
            |:||.||||||                          .||||||.||||     |||
Yeast  1544 PTSPTYSPTSP--------------------------AYSPTSPSYSPT-----SPS-------- 1569

  Fly  1665 YSPGNAYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSP 1729
                  |||:|.:|||.|||||||||||||:|||||||||.|||||||||||||:|:|.:|||||
Yeast  1570 ------YSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP 1628

  Fly  1730 TSPNYSASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPT 1794
            |||:                    ||||||:|||.||||  ||.||.|:|.||.|||.||.||||
Yeast  1629 TSPS--------------------YSPTSPSYSPTSPSY--SPTSPSYSPTSPAYSPTSPSYSPT 1671

  Fly  1795 SPLYSPSSPQHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPM 1859
            ||.|||:||                                  .||||||:|:||:.|||||||.
Yeast  1672 SPSYSPTSP----------------------------------SYSPTSPSYSPTSPNYSPTSPS 1702

  Fly  1860 YSPTAPSHYSPTSPAYSPSSPTFEESED 1887
            ||||:|. |||.||||||.....:.:|:
Yeast  1703 YSPTSPG-YSPGSPAYSPKQDEQKHNEN 1729

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr2A	NP_511124.1	RNAP_II_RPB1_N	15..868	CDD:259848	479/858 (56%)
		RNA_pol_Rpb1_6	890..1071	CDD:461511	65/188 (35%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	216/423 (51%)
		Herpes_BLLF1	<1498..1862	CDD:282904	155/375 (41%)
		PTZ00449	<1707..1880	CDD:185628	89/172 (52%)
RPO21	NP_010141.1	RNAP_II_RPB1_N	15..853	CDD:259848	479/858 (56%)
		RNA_pol_Rpb1_6	873..1056	CDD:461511	65/190 (34%)
		RNAP_II_Rpb1_C	1035..1446	CDD:132720	216/423 (51%)
		RNA_pol_Rpb1_R	1698..1709	CDD:461513	8/10 (80%)