Protein Alignment Polr2A and POLR2A

DIOPT Version :10

• Sequence 1: NP_511124.1
• Gene: Polr2A / 32100
• FlyBaseID: FBgn0003277
• Length: 1887
• Species: Drosophila melanogaster
• Sequence 2: NP_000928.1
• Gene: POLR2A / 5430
• HGNCID: 9187
• Length: 1970
• Species: Homo sapiens
• Alignment Length: 1926
• Identity: 1404/1926 - (72%)
• Similarity: 1640/1926 - (85%)
• Gaps: 54/1926 - (2%)

  Fly     6 DSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTC 70
            ||..|||.:||||||:|||||::||||||||:::.||.||||||||||||||||||:||.|||||
Human    10 DSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTC 74

  Fly    71 AGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRK 135
            ||||||||||||||:|||||||:||:.||:|:||||||:|||:||..:|||||:|:.||:|||:|
Human    75 AGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKK 139

  Fly   136 RLAYVYDLCKGKTICEGGEDMDLTKENQQP----DPNKKPGHGGCGHYQPSIRRTGLDLTAEWKH 196
            ||.:||||||||.||||||:||.....:||    |..|:.||||||.|||.|||:||:|.|||||
Human   140 RLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKH 204

  Fly   197 QNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFGA 261
            .|||||||||::|.|||.||.|.|:|||||:|||:|:||||:||||||||||||:|||||||.|:
Human   205 VNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGS 269

  Fly   262 AKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGK 326
            |:|||||||||:||:|.||:||:||.:|||||||.|::|:|||||||:|||::||:||||||||:
Human   270 ARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGR 334

  Fly   327 PLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNI 391
            |||::|.||||||||:|||||||||||||||||||||||.|||||||||||.|:||.|:||||||
Human   335 PLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNI 399

  Fly   392 DRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTL 456
            ||:|||||||||||||||||:||||:|||||||||.||||||.|||||||:.|.|:|||||||||
Human   400 DRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTL 464

  Fly   457 HKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQ 521
            ||||||||||::|||||||:|||.|:||||||||||||||:|||:|||||::.:.:.||.|:|||
Human   465 HKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQ 529

  Fly   522 ANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSL 586
            :|:|||||||||||||||.||||||:.|.:||||||||.|||.|:|||.||||||||||||||||
Human   530 SNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSL 594

  Fly   587 IIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLE 651
            ||||::|.|||||||||:||.||||.||||||||:||:||||||||||||||||||||:||.:||
Human   595 IIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLE 659

  Fly   652 LGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNME 716
            :||||...||.|||||||||||.|||:|||||:|||.:||.:||..|||||.|||.||:||||.|
Human   660 MGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNE 724

  Fly   717 LEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVG 781
            |||||||||||||||:|||||||||||||.||:|||:||||.|:|||||:|||.||||||||.||
Human   725 LEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVG 789

  Fly   782 QQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKT 846
            ||||||||||:||:.|||||||||||||||||||||||||||||:||:|||||||||||||||||
Human   790 QQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKT 854

  Fly   847 AETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKR 911
            ||||||||||||:||||||.||.|||||:.|::|||||||||.||.|||||:.|:|.|||:|||:
Human   855 AETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKK 919

  Fly   912 FKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQ 976
            |:||::|||.:::...:|::|::..::....|||.|::|:..||:.||.|||.|:|||||||||.
Human   920 FRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLL 984

  Fly   977 RMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIRSTL 1041
            |||||.|||||||.|||:||.||:|::|||.|.::.|||.|:|.:|:||.|||||||...:||||
Human   985 RMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTL 1049

  Fly  1042 CTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSS 1106
            |::.::||||||.|||:||:||||::|.||.|:||||||||||||||||||||||||||:||||:
Human  1050 CSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSA 1114

  Fly  1107 KNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDP 1171
            ||||||||||||:||||||||.|||||||.|.:|||||:||::||||||||||||||||||||||
Human  1115 KNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDP 1179

  Fly  1172 DPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQIAEKINVGFGEDLN 1236
            :||.||::||||:|||||||||||..||||||||:|||||.|||:||||||||||||.|||:|||
Human  1180 NPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLN 1244

  Fly  1237 CIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGIEAIGKVYM 1301
            ||||||||:||||||||||::|||.|:|:|.||||:||:||||||:|||:||||||||.|.||||
Human  1245 CIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYM 1309

  Fly  1302 HLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGIEAV 1366
            ||||||:||:|:|||.|||||:.||:|||||.|:|:||||:||||:||:||||.|||.|||||||
Human  1310 HLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAV 1374

  Fly  1367 RKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVL 1431
            ||::|:|:..|:.|.|.||||||||||||.||.:||||||||||:||||||.||:||||||||||
Human  1375 RKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVL 1439

  Fly  1432 MDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIP-NTLGNSMLGGAAMFIG 1495
            |:||||.|:|||:||||||::|||...||||||||||||||::|:||| |..|....|...||.|
Human  1440 MEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFG 1504

  Fly  1496 GGSTP--SMTPPMTPWANCNTPRYFS-PPGHVSAMTPGGPSFSPSAASDASGMSPSWSPA---HP 1554
            ...:|  .::|.||||....||.|.: .|...|.||||...|||||||||||.||.:|||   .|
Human  1505 SAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTP 1569

  Fly  1555 GSSPSSPGPSMSPYFPASP--SVSPSYSPTSPNYTASSPGG-----------------ASPNYSP 1600
            | ||.||||| |||.| ||  ::|||||||||.|...||||                 .||:|||
Human  1570 G-SPGSPGPS-SPYIP-SPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSP 1631

  Fly  1601 SSPNYSPTSPLYA--SPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQS--SPSFAGSG 1661
            :|||||||||.|:  ||.|:.|:|:::|.|..|||:|..||||||.||||....|  |||::.:
Human  1632 TSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT- 1695

  Fly  1662 SNIYSPGN-AYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTP 1725
            |..|||.: :|||:|.:|||.|||||||||||||:|||||||||.||||||:||||||||||.:|
Human  1696 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSP 1760

  Fly  1726 SYSPTSPNYS-ASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGS------------PGSPQY 1777
            |||||||:|| .||.|:|.||.||.|...||||||:|||.||||..|            |.||.|
Human  1761 SYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSY 1825

  Fly  1778 TPGSPQYSPASPKYSPTSPLYSPSSPQHSP-SNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSP 1841
            :|.||.|||.||||:||||.||||||:::| |.:||||...||.|||:|||....|||::.||||
Human  1826 SPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSP 1890

  Fly  1842 TSPTYTPTARNYSPTSPMYSPTAPSHYSPTSPAYSPSSPTF 1882
            |||||:||:..|:||||.||||:|: ||||||.|||:|||:
Human  1891 TSPTYSPTSPVYTPTSPKYSPTSPT-YSPTSPKYSPTSPTY 1930

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr2A	NP_511124.1	RNAP_II_RPB1_N	15..868	CDD:259848	685/856 (80%)
		RNA_pol_Rpb1_6	890..1071	CDD:461511	105/180 (58%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	334/417 (80%)
		Herpes_BLLF1	<1498..1862	CDD:282904	240/407 (59%)
		PTZ00449	<1707..1880	CDD:185628	119/186 (64%)
POLR2A	NP_000928.1	RNAP_II_RPB1_N	19..876	CDD:259848	685/856 (80%)
		Bridging helix. /evidence=ECO:0000250|UniProtKB:G3MZY8	832..873		37/40 (93%)
		RNA_pol_Rpb1_6	896..1079	CDD:461511	106/182 (58%)
		RNAP_II_Rpb1_C	1058..1476	CDD:132720	334/417 (80%)
		Trigger loop. /evidence=ECO:0000250|UniProtKB:G3MZY8	1083..1124		38/40 (95%)
		CTD	1491..1610	CDD:215026	65/121 (54%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1546..1970		241/390 (62%)
		C-terminal domain (CTD), 52 X 7 AA approximate tandem repeats of Y-[ST]-P-[STQ]-[ST]-P-[SRTEVKGN]	1593..1960		207/340 (61%)
		Herpes_BLLF1	<1854..>1959	CDD:282904	50/78 (64%)