Protein Alignment Polr2A and nuc1

DIOPT Version :10

• Sequence 1: NP_511124.1
• Gene: Polr2A / 32100
• FlyBaseID: FBgn0003277
• Length: 1887
• Species: Drosophila melanogaster
• Sequence 2: NP_596300.1
• Gene: nuc1 / 2540873
• PomBaseID: SPBC4C3.05C
• Length: 1689
• Species: Schizosaccharomyces pombe
• Alignment Length: 1793
• Identity: 474/1793 - (26%)
• Similarity: 758/1793 - (42%)
• Gaps: 463/1793 - (25%)

  Fly    13 QVKRVQFGILSPDEIRRMSVTEGGVQFAETMEG-GRPKLGGLMDPRQGVIDRTSRCQTCAGNMTE 76
            ::|.|:|||...|::.::||.:  :.....::. ..|..|||.|...|...:.|.|.||..:...
pombe    10 EIKSVKFGIYDVDDVEKISVKQ--IVNPVLLDNLNHPTNGGLYDLALGPYLKNSVCATCHLDERY 72

  Fly    77 CPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSP------------------------ 117
            ||||||||.|..|.:|..|.::...:||..|.||....:|.
pombe    73 CPGHFGHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVKVHLFFCRLKLLDYGLLNESEMV 137

  Fly   118 HNPKIKEIVMK-SRGQPRK--------------------RLAYVYDLCKGKTICEGGE----DMD 157
            .|..:.|.::| |.|.|.:                    .|..:.|....|:|.:..:    |..
pombe   138 ENVSLTEAIIKNSNGTPLEDGSDSEDSGLGHDDIAKDAATLMRIRDEFVAKSIADSRQNAHIDAQ 202

  Fly   158 LT------------------KENQQPDPNKKPGHGGCGHYQPSIRRTG----------------- 187
            ||                  ...:|.|        .|..:.|:.|:.|
pombe   203 LTTLLLHERKKVVRAFYHAISSRKQCD--------NCQSFSPNFRKEGFAKIFEIPLSGKNLQFM 259

  Fly   188 ----------LDLTAEWKHQNE------DSQEK----------------------------KIVV 208
                      |..|::..|::|      ||..:                            |.:.
pombe   260 EQTGKIRSDVLRDTSKKHHEDEGYDGDSDSSNESEVEGIDLFEEDPNPLKNKSKSPIAHGAKYMT 324

  Fly   209 SAERVWEILKHITDEECFILG-----MDPKYARPDWMIVTVLPVPPLAVRPAVVMFGAAKNQDDL 268
            |.| |...|:.:..:|..:|.     ...|.|..|...:..:.|||...|||..| |...:::..
pombe   325 STE-VRNHLRRLFVKENVVLSRLYAHKRGKPASADMFFLQNIAVPPTRFRPASKM-GDEVHENIQ 387

  Fly   269 THKLSDIIKAN-----------NELRKNEASG-----AAAHVIQENIKMLQFHVATLVDNDMPGM 317
            ...|:.|::::           .|:..:|..|     .|..::......||..|.:|:|::....
pombe   388 NELLTRILQSSIQIASLSKDSTVEVNPDEKEGLERRSRAFELLINAFVQLQHDVNSLIDSNRNPS 452

  Fly   318 PRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTF 382
            .....::..|  .||..|:.|||..|.::|||||:::||:||:||||:..:::|||...|..||:
pombe   453 SGGQSRTVPP--GIKQILEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFATKLTY 515

  Fly   383 PELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLR--------------FHPKSSDLHLQ 433
            ||.||.:|.:.|:..|..|..::|||.:|..::|..|.|.              ..|:|:.:...
pombe   516 PEPVTLYNFNEMRNAVINGPHKWPGASHIQNEDGTLISLMPLTIEQRTALANQLLTPQSNLISSP 580

  Fly   434 CGY--------KVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPW-STFRMNLSCTSPYNADFD 489
            ..|        ||.||:|:.|::|.||||||||.|||.|:.::||. .|.||:.:..:.||||||
pombe   581 YSYSRLINTNKKVYRHVRNGDMLILNRQPTLHKPSMMAHKARILPGEKTIRMHYANCNSYNADFD 645

  Fly   490 GDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMN 554
            |||||:|.|||...|:|.:.|..|..|.:.|.:..|:.|::||.:.....:|.:|.|.||::...
pombe   646 GDEMNMHFPQSTNARSEAQFIANTDSQYLVPTSGDPLRGLIQDHVVMGVWLTCKDTFYTRDEYQQ 710

  Fly   555 LLMFLPTWDAKMPQ------------PCILKPRPLWTGKQIFSLII--------PGNVNMIRTHS 599
            ||     :.|..|.            |.|.:|...||||||.|.::        || :|:
pombe   711 LL-----FQALKPDETGMYGRIKTLPPAIQRPGIYWTGKQIISSVLLNLKPSDRPG-LNL----- 764

  Fly   600 THPDEEDEGPYKWISPG--DTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIAGRFYG 662
               ..:.:.|.|:.||.  :..|:.:.|||:.|||.|.|.|.||..|:|......|.|||||...
pombe   765 ---KSKAKVPGKYWSPDSEEGSVLFDDGELLCGILDKSSFGASAFGLVHSVHELYGPDIAGRLLS 826

  Fly   663 NIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAK---------------DDVINVIQKA 712
            .:..:...:....|.:..:.|...|.|..|..:|.::..|               |..|.::
pombe   827 VLSRLFTAYAQMRGFTCRMDDLRLDEQGDNWRRQLLENGKSFGLEAASEYVGLSTDSPIALL--- 888

  Fly   713 HNMELEPTPGNT-----LRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNIN 772
             |..||....:.     |....:.|:|.:.:...:|.......:...||:::.|.|||:||||:|
pombe   889 -NANLEEVYRDDEKLQGLDAAMKGKMNGLTSSIINKCIPDGLLTKFPYNHMQTMTVSGAKGSNVN 952

  Fly   773 ISQVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGRE 837
            :||:...:|||.:||:|:|.....::||.|:..:...:|.||:.:.:|.|:.|.|:|||.|.|||
pombe   953 VSQISCLLGQQELEGRRVPLMVSGKSLPSFVPYETSAKSGGFIASRFLTGIAPQEYYFHCMAGRE 1017

  Fly   838 GLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVK 902
            |||||||||:.:||:||.|:|.:|.:.|.||.|||:|.|.::|..||||.|  ::.:.:::...:
pombe  1018 GLIDTAVKTSRSGYLQRCLMKHLEGLCVQYDHTVRDSDGSIVQFHYGEDSL--DVTKQKHLTQFE 1080

  Fly   903 LSNKSFE---KRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPN 964
            .|.|:::   :::|        :|.|.:  .:...|.||.|.:.|:..:     ..|.:...:|
pombe  1081 FSAKNYKSLIQKYK--------VKSVLS--AVDSETASSYAKKALKKPY-----KYDPVLDKYP- 1129

  Fly   965 GESKVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENA 1029
                                             |.|.:..|....:|.|        .:...:|.
pombe  1130 ---------------------------------PSRYLGSVSEKFQRAV--------DEYTQKNP 1153

  Fly  1030 TLLFQCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQM 1094
            ..|......|.|      ::..|:...|:.|   ::.|:||:..:|||.||.||:||:|||:|||
pombe  1154 DKLIASKKESKL------DDSLLNESKFKAL---MQLRYQQSLVDPGESVGVLASQSIGEPSTQM 1209

  Fly  1095 TLNTFHFAGVSSKNVTLGVPRLKEII-NISKKPKAPSLTVFLTGGAARDAEKAKNVLCR-----L 1153
            |||||||||..:||||||:|||:||| ..|...:.|::|:.|..|.   ::|..:..|:     :
pombe  1210 TLNTFHFAGFGAKNVTLGIPRLREIIMTASANIQTPTMTLRLNDGV---SDKRASAFCKEVNKLV 1271

  Fly  1154 EHTTLRKVTANTAIYYDPDPQRT--------VISEDQEFVNVYYEMPDFDPTRISPWLLRIELDR 1210
            ....:|:|.....|......:::        :.|.| |:.:.|..:.:...:..|...|:| |:|
pombe  1272 LSEVVRQVRVTEKISGQGSDEQSKTYAIRLDLYSRD-EYQDEYGVLQEEIESTFSNRFLKI-LNR 1334

  Fly  1211 -----------KRMTDKKLTMEQIAEKINVGFGEDLN----------CIFNDDN----------- 1243
                       ::...|..|:.::.:.:..  .||::          .:.::||
pombe  1335 IIKSYLAKSKQRKSGGKDDTVPEVGQALKP--LEDIDEAPIEGRAQEALEDEDNDATNEKMVSRS 1397

  Fly  1244 -----------ADKLVLR-IRIMNNEENKFQDE--------DEAVD------------------- 1269
                       |||:.|| ::..|..|:...||        ||:|.
pombe  1398 KQHASYEGPDEADKVALRQLKGSNKVEDVNMDEEEDEGFKSDESVSDFKERKLLEKQNTVSISER 1462

  Fly  1270 -----KMEDDMFLRC--------------------IEANML----------SDMTLQGIEAIGKV 1299
                 |...::...|                    |....|          |:..:..|..|.:.
pombe  1463 RELQLKTAKEILSNCKHLDFDYVNGEWATVELVFPINTEKLLMVSLVEKACSETVIHEIPGITRC 1527

  Fly  1300 YMHLPQT--DSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLG 1362
            :...|.:  |:..: ||||....|||.|:..|         :|..|:     .:|||..|.::.|
pombe  1528 FSKPPDSALDTVPK-VITEGVNLKAIWEFYNE---------ISMNDI-----YTNDIAAILRIYG 1577

  Fly  1363 IEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEET 1427
            :||.|.::..|:::|...||:.|:.|||:|:.|.||.:|...|..|.|| ..:|....:.|||.|
pombe  1578 VEAARNAIVHEVSSVFGVYGIAVDPRHLSLIADYMTFEGGYKAFNRMGI-EYNTSPFAKMSFETT 1641

  Fly  1428 VDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDL 1465
            ...|.:||...:.|.:...|..:::|::...|||.||:
pombe  1642 CHFLTEAALRGDVDDLSNPSSRLVVGRVGNFGTGSFDI 1679

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr2A	NP_511124.1	RNAP_II_RPB1_N	15..868	CDD:259848	300/1067 (28%)
		RNA_pol_Rpb1_6	890..1071	CDD:461511	26/183 (14%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	140/538 (26%)
		Herpes_BLLF1	<1498..1862	CDD:282904
		PTZ00449	<1707..1880	CDD:185628
nuc1	NP_596300.1	RNAP_I_RPA1_N	15..1048	CDD:259844	298/1064 (28%)
		RNA_pol_Rpb1_5	1002..1633	CDD:398596	186/721 (26%)