Protein Alignment Upf2 and Upf2

DIOPT Version :10

• Sequence 1: NP_572434.1
• Gene: Upf2 / 31724
• FlyBaseID: FBgn0029992
• Length: 1241
• Species: Drosophila melanogaster
• Sequence 2: NP_001074601.1
• Gene: Upf2 / 326622
• MGIID: 2449307
• Length: 1269
• Species: Mus musculus
• Alignment Length: 1260
• Identity: 464/1260 - (36%)
• Similarity: 697/1260 - (55%)
• Gaps: 164/1260 - (13%)

  Fly    42 DANADEDVDAAAAIALEAEEREELQQFISELREKIESKRQLRL--QNSTIELPGEEYFAKLDSNL 104
            :..|....:.|||   :.:|:||..|...|..|:.:.:::||.  ||:....|.|.:|::|||:|
Mouse   106 EEQAKRQQEEAAA---QLKEKEESLQLHQEAWERHQLRKELRSKNQNAPDNRPEENFFSRLDSSL 167

  Fly   105 KKNTAFVKKLKLFTATQLDGLLREISALNLSKYISEICAALVEAKLKMTDVPAVVTLASRLHCTY 169
            |||||||||||..|..|.|.|..:.:.|||||||:|..|::||||||::||.....|.|..|..|
Mouse   168 KKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKLSDVNCAAHLCSLFHQRY 232

  Fly   170 ADFDVHFLEAWQKALNIKATEKIGNPSKLRVDLRLFAELVSSGVIQMKPGLAQLGVVLVHLIALD 234
            :||....|:.|:|....:..||..|.:|||.|||..|||...|:...|.||:.:...|..:|..|
Mouse   233 SDFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDKEGLSLIYEQLKSIINAD 297

  Fly   235 KDDHSNFSIILSFCRHCGEEYAGLVPQKMQQLATKYGVEVPKSDFLTADKQLNLRTMLKGYFKAL 299
            ::.|::.|:::|||||||::.|||||:|::..|.|:.:..|.|:.::.:||...:.:||.||.:|
Mouse   298 RESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQPFQNLLKEYFTSL 362

  Fly   300 CKHVLAEQAELMNMTKNIRRTMECKGEISTEKREKCELMQAGFDKLLASAQSLSELLGEPLPELA 364
            .||:..:..||.|..:..||.:..|||:|.::.::.|.....:.||||::|||::||.|.:|:|.
Mouse   363 TKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLP 427

  Fly   365 KES---EGCNPGTVIDNMLDSASFGVLD----PWGDEETRAFYTDLPDLRQFLP----------- 411
            ::.   |...||  || :......|..|    .|.||:.|.||.:|.||:.|:|
Mouse   428 QDKPTPEEHGPG--ID-IFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQ 489

  Fly   412 NFSAPKVDLETLEEPSELTEEAIDANLDAE---MDLDDPPSTTSDTTPENPIEEQPTTPVAAAED 473
            |..:.|.|.:..:||.:..|.:...:|:.|   ::::|      ||......:|        |||
Mouse   490 NKDSNKDDSKEAKEPKDNKEASSPDDLELELENLEIND------DTLELEGADE--------AED 540

  Fly   474 LKPQKMGNALMELGRQQQQQSQLNQNPIQIQNQMRQQFDGFLVNLFNCVNKELIDSAAIEFLLNF 538
            | .:|:      |..|:|:..:.:..     :.::...|.||..|.||||::|||.||::|.:|.
Mouse   541 L-TKKL------LDEQEQEDEEASTG-----SHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNM 593

  Fly   539 NTKHQRKKLTRTIFSVQRTRLDILPYLSRFVAIVHMCNTDVATDLAELLRKEFKWHIHKKNQLNI 603
            |||..||||.|.:|.|.|.|||:||:.:|.||.:|.|.:|||.||..:||.:|::|:.||:|:||
Mouse   594 NTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINI 658

  Fly   604 ETKLKIVRFIGELVKFGLFKKFDALGCLKMLLRDFQHHQIEMACAFVEVTGVYLYNCRDARLLMN 668
            |||.|.|||||||.||.:|.|.|.|.||||||.||.||.|||||..:|..|.:|:...::.|..:
Mouse   659 ETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTS 723

  Fly   669 VFLDQMLRLKTATAMDSRHAAQIESVYYLVKPPESSKREPMVRPAMHEYIRYLIFEELCKQNVDR 733
            |.|:||:|.|.|..:|:|:...:|:.||...||.:.|.....||.:.||:|.|::::|.|...::
Mouse   724 VLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRKKRPPLQEYVRKLLYKDLSKVTTEK 788

  Fly   734 CIKMLRRIDWQDPETNCYAIKCLSKAYLLRFQLIRCLADLVSGLSSYQPRAVTIVIDNVFEDIRA 798
            .::.:|::.|||.|...|.|.|:...:.:::..|.|:|:|::||..||......|:|.|.||||.
Mouse   789 VLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRL 853

  Fly   799 GLEIHSPRMAQRRIAMAKYLGEMYNYKLVESTNILNTLYSIISLGVSNDQNVVSPLDPPDSLFRL 863
            |:|::.|:..||||:.||:|||:|||::|||..|..||||..|.||:.|.: .|.||||:.|||:
Mouse   854 GMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGS-PSSLDPPEHLFRI 917

  Fly   864 KLACMLLDTCAPYFTSQATRKKLDYFLVFFQHYYWFKKSHPVFSKTENTSDLFPILMDHTYRDCL 928
            :|.|.:||||..||...::::|||.|||:||.|.|:|||..|::|...    |||.:|:...|.|
Mouse   918 RLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHP----FPIDIDYMISDTL 978

  Fly   929 LNVRPKLKIYKSLEQAKAAIDHLQEKLYPQLKTTNNAQDPSLGT----ISEISEIDEGG--TDED 987
            ..:|||:|:..|||::...:..|:.:...:|...|:.:.....|    :.|..|.:|||  |:|.
Mouse   979 ELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQ 1043

  Fly   988 SGSSNDQR--------ERQVSGQEQDQSN-DW-----TENEAEPP----------LPPLPPPEKS 1028
            ||:.::..        |.:..|:|:::.| |:     .|||.:..          |..:|    .
Mouse  1044 SGNESEVNEPEEEEGSEEEEEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVP----C 1104

  Fly  1029 KEDLEFEQLYEKMTTDSYQERLKEPIKATAKDIPVPMMARLQKKSYDQITGAQTIGPGINNMSQI 1093
            .||.:|.|..:||..::.|:|..|.:|....|:.:|:..:                      ||:
Mouse  1105 VEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLK----------------------SQL 1147

  Fly  1094 SKSVPNQDGTPGSPGADSPDGNGKSGGGVGGSDVGAAGAAGASGATSVPFVLMVRGHKGGKQQFK 1158
            .|..|                   .|||.|.::          .|.::|||::.|  ||.|||||
Mouse  1148 RKGPP-------------------LGGGEGETE----------SADTMPFVMLTR--KGNKQQFK 1181

  Fly  1159 SFVAPSDSHLATNLKRQEQMIREEKEKVKRLTLNITERIEEEDYQESLMPPQHRNFTQSYYQKP- 1222
            ....|..|.||.|...|:|..:||:.::|:|||:|.||.|:|||||.|         ||..|:|
Mouse  1182 ILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEML---------QSLAQRPA 1237

  Fly  1223 -------NKHKFKHQKGAPDADLIF 1240
                   .:.:::|.||||:|||||
Mouse  1238 PANTNRERRPRYQHPKGAPNADLIF 1262

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Upf2	NP_572434.1	MIF4G	104..300	CDD:397130	86/195 (44%)
		MIF4G	518..701	CDD:214713	95/182 (52%)
		MIF4G	716..934	CDD:397130	94/217 (43%)
		Upf2	1029..1192	CDD:461146	44/162 (27%)
Upf2	NP_001074601.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..125		4/18 (22%)
		Sufficient for interaction with UPF1. /evidence=ECO:0000250|UniProtKB:Q9HAU5	94..132		7/25 (28%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	143..162		6/20 (30%)
		MIF4G	167..362	CDD:214713	86/194 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	422..444		8/24 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	487..518		6/33 (18%)
		MIF4G	569..756	CDD:214713	95/186 (51%)
		Sufficient for interaction with UPF3A and UPF3B. /evidence=ECO:0000250|UniProtKB:Q9HAU5	709..926		86/216 (40%)
		Sufficient for interaction with EIF4A1 and EIF1. /evidence=ECO:0000250|UniProtKB:Q9HAU5	755..1269		184/543 (34%)
		MIF4G	774..984	CDD:397130	92/209 (44%)
		Binds to UPF3B. /evidence=ECO:0000250|UniProtKB:Q9HAU5	837..857		7/19 (37%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1017..1090		22/102 (22%)
		Sufficient for interaction with UPF1 C-terminus. /evidence=ECO:0000250|UniProtKB:Q9HAU5	1081..1269		55/197 (28%)
		Upf2	1086..1215	CDD:461146	26/128 (20%)
		Necessary for interaction with UPF1. /evidence=ECO:0000250|UniProtKB:Q9HAU5	1102..1195		16/92 (17%)
		Interaction with UPF1. /evidence=ECO:0000250|UniProtKB:Q9HAU5	1102..1126		7/23 (30%)
		Interaction with UPF1. /evidence=ECO:0000250|UniProtKB:Q9HAU5	1164..1204		5/39 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1218..1269		26/50 (52%)