Protein Alignment Vinc and VCL

DIOPT Version :10

• Sequence 1: NP_476820.1
• Gene: Vinc / 31201
• FlyBaseID: FBgn0004397
• Length: 961
• Species: Drosophila melanogaster
• Sequence 2: NP_054706.1
• Gene: VCL / 7414
• HGNCID: 12665
• Length: 1134
• Species: Homo sapiens
• Alignment Length: 1148
• Identity: 469/1148 - (40%)
• Similarity: 647/1148 - (56%)
• Gaps: 202/1148 - (17%)

  Fly     1 MPVFHTKTIESILDPVAQQVSRLVILHEEAE-DGNAMPDLSRPVQVVSAAVANLVKVGRDTINSS 64
            ||||||:||||||:|||||:|.|||:|||.| ||.|:|||:.||..|.|||:|||:||::|:.::
Human     1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTT 65

  Fly    65 DDKILRQDMPSALHRVEGASQLLEEASDMLRSDPYSGPARKKLIEGSRGILQGTSSLLLCFDESE 129
            :|:||::|||.|..:||.|...|.:|:.||:|||||.|||..||:||||||.|||.|||.|||:|
Human    66 EDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAE 130

  Fly   130 VRKIIQECKRVLDYLAVAEVINTMEQLVQFLKDLSPCLSKVHREVGAREKELTHQVHSEILVRCL 194
            |||||:.||.:|:||.||||:.|||.||.:.|:|.|.::|:.:.:..|::|||||.|..:||..:
Human   131 VRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSM 195

  Fly   195 EQVKTLAPILICSMKVYIHIVEQQGRGAEEAAENRNYLAARMSDELQEIIRVLQLTTYDEDTSEL 259
            ..||.|.|:||.:||:::.....:.:|.|||.:|||:...:||.|:.|||||||||::|||....
Human   196 NTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWAS 260

  Fly   260 DNLTVLKKLSNAISNKMEQANEWLSNPYALRGGVGEKALRQVIDNATEISERCL----------- 313
            .:...:|:...:|.:|:.||..||.:|.|..|..||:|:||::|.|.::.|.|.
Human   261 KDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTC 325

  Fly   314 ----------------PQDSYPI------------------------------------------ 320
                            .|.|.|:
Human   326 KMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDA 390

  Fly   321 -------------------------RKLAD----------------EVTAMANTLCELRQEGKGQ 344
                                     ||:|:                |::|:.:.|.:||::|||.
Human   391 AQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGD 455

  Fly   345 SPQAESLVRGIRDRMGELKSLVHQAVLGVDKAGVQQTAHTIQGRLEQAVKWLQHPEINDGGLGER 409
            ||:|.:|.:.:...:..|::..::||.....|   :.|..::|::|||.:|:.:|.::|.|:|:.
Human   456 SPEARALAKQVATALQNLQTKTNRAVANSRPA---KAAVHLEGKIEQAQRWIDNPTVDDRGVGQA 517

  Fly   410 AINLIVEEGRKVAEGCPGHQKAEIQQLCDEVERLKRQAA-------GSGPAAKQAAKQLTQKLYE 467
            ||..:|.||.::|....|..:.::...||.|::|..|.|       |..|.|:..|.||...|.:
Human   518 AIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKD 582

  Fly   468 LKAAIQNALVNRIVQDFMDVSTPLKQFTEAVLQPEGTPGREQNFNQKSNNLQAFSDRASKTSRMV 532
            |||.:|.|:...:...|.|.:||:|....|...|...|.||:.|::::.|.:..|.:...|:...
Human   583 LKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKA 647

  Fly   533 AAGGACGNKKIAEILLSSAAQVDSLTPQLISAGRIRMNYPGSKAADEHLQNLKQQYADTVLRMRT 597
            ||.|. .||...|.:.:|......||||::||.||.:..||::||.||.:.:|.|:.|.|.:|..
Human   648 AAVGT-ANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTG 711

  Fly   598 LCDQATDPADFIKTSEEHMQVYAKLCEDAIHARQPQKMVDNTSNIARLINRVLLVAKQEADNSED 662
            |.|:|.|....:..|||.::.....|:.|:...|||.:|...::|||..||:|||||:|.:||||
Human   712 LVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSED 776

  Fly   663 PVFTERLNAAANRLERSLPAMVGDAKLVATNIADPAAAAAWKNSFQRLLGDVREVRDAIAPPQPP 727
            |.|.|.:.||::.|.:::..||.|||.||.||:||....::.:|..|:||.|.:||:|..|.:|
Human   777 PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEP- 840

  Fly   728 PLPTSLPPPIPELSALHLSNQNAERAPPRPPLPREGLAPVRPPPPETDDED-------------- 778
                ..|||.|:|..|.|::   |.|||:||||...:.|.||||||..||:
Human   841 ----DFPPPPPDLEQLRLTD---ELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPM 898

  Fly   779 ---------------------------------------------------EGVFRTMPH---AN 789
                                                               |.....||.   .|
Human   899 MMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVN 963

  Fly   790 QPILIAARGLHQEVRQWSSKDNEIIAAAKRMAILMARLSELVLSDSRGSKRELIATAKKIAEASE 854
            ||||.||:.||:|..:||||.|:|||||||||:|||.:|.||...| |:||.||..||.||:||:
Human   964 QPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGS-GTKRALIQCAKDIAKASD 1027

  Fly   855 DVTRLAKELARQCTDRRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQG-SDEDRE-ATEMLV 917
            :|||||||:|:||||:||||||||||||||||.|||||||||||||||... |||:.| ||||||
Human  1028 EVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLV 1092

  Fly   918 GNAQNLMQSVKETVRAAEGASIKIRSDQTSNRLQWVRRQPWYQ 960
            .||||||||||||||.||.||||||:| ....|:|||:.||||
Human  1093 HNAQNLMQSVKETVREAEAASIKIRTD-AGFTLRWVRKTPWYQ 1134

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Vinc	NP_476820.1	Vinculin	3..960	CDD:460040	465/1144 (41%)
VCL	NP_054706.1	N-terminal globular head. /evidence=ECO:0000269|PubMed:20484056	1..835		313/837 (37%)
		Vinculin	3..1134	CDD:460040	465/1144 (41%)
		Talin-interaction. /evidence=ECO:0000250|UniProtKB:P12003	168..208		16/39 (41%)
		3 X 112 AA tandem repeats. /evidence=ECO:0000255	259..589		74/332 (22%)
		Interaction with ACTN4. /evidence=ECO:0000269|PubMed:15988023, ECO:0007744|PDB:1YDI	741..764		9/22 (41%)
		Linker (Pro-rich). /evidence=ECO:0000269|PubMed:20484056	836..878		21/49 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	857..887		17/29 (59%)
		C-terminal tail. /evidence=ECO:0000269|PubMed:20484056	879..1134		130/256 (51%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1003..1046		27/43 (63%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1120..1134		6/13 (46%)