Protein Alignment CHRNB2 and nAChRalpha3

DIOPT Version :10

• Sequence 1: NP_000739.1
• Gene: CHRNB2 / 1141
• HGNCID: 1962
• Length: 502
• Species: Homo sapiens
• Sequence 2: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Alignment Length: 760
• Identity: 244/760 - (32%)
• Similarity: 331/760 - (43%)
• Gaps: 297/760 - (39%)

Human    10 LLLGFGLLRLCSGVWGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHERE 74
            |:|.|    :.|.....|. :||.:.||  |.||||:||..|.::.:||::.:.|:|||.|:.:.
  Fly    12 LVLSF----ISSSTANPDA-KRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKN 69

Human    75 QIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSY 139
            ||||||:|:.|.|.||:|.|:|:|:..::.:.:||.|||.||:||||||||.:||:..:.|.::|
  Fly    70 QIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNY 134

Human   140 DGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEV---------ASLD 195
            .|.:.|.|||||||:|:|:|::||||:|.|.|||.|||||..::||....|:         ..|.
  Fly   135 TGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLS 199

Human   196 DFTPSGEWDIVALPGRRNE---NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFY 257
            :|..|.||||:.:|..|||   ...|..|:|||::..:|||.||||:||||||:.|:.|.|||||
  Fly   200 EFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFY 264

Human   258 LPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVH 322
            ||||.|||::|.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.|||:|
  Fly   265 LPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIH 329

Human   323 HRSPTTHTMAPWVKVVFLEKLPALLFMQQP----------------------------------- 352
            .|||.||||||||:.||:.:||..|.|::|
  Fly   330 FRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPV 394

Human   353 ----RHHCARQRLR-----LRRR------------------------------------------ 366
                .||..:...|     |:.|
  Fly   395 ALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTST 459

Human   367 -----------QREREGAGALF--------FREAPG----------------------------- 383
                       |.::.| |:|.        ||:...
  Fly   460 TSSLIDAQYHNQYQQTG-GSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHH 523

Human   384 ------------------------------------------------------ADSC---TCFV 391
                                                                  ||||   |..:
  Fly   524 LYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQI 588

Human   392 NR--------------------------------------------------------------- 393
            ::
  Fly   589 HQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAA 653

Human   394 ASVQGLAGAFGAEPAPVAGPG---------RSGEPCGCG--------------LREAVDGVRFIA 435
            |:....|.|.|..|.|....|         |.|.|..|.              |.:|:|||.:||
  Fly   654 AAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIA 718

Human   436 DHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQ 480
            |..|.|::...|.|||||||||:||||||||....|.||.|:.||
  Fly   719 DQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CHRNB2	NP_000739.1	LIC	29..479	CDD:455710	237/738 (32%)
		LGIC_ECD_nAChR_B2	31..234	CDD:349826	100/214 (47%)
		Cys-loop	155..169	CDD:349826	8/13 (62%)
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	102/220 (46%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	59/88 (67%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	35/76 (46%)