Protein Alignment CFTR and Sur

DIOPT Version :10

• Sequence 1: NP_000483.3
• Gene: CFTR / 1080
• HGNCID: 1884
• Length: 1480
• Species: Homo sapiens
• Sequence 2: NP_001334732.1
• Gene: Sur / 34350
• FlyBaseID: FBgn0028675
• Length: 2223
• Species: Drosophila melanogaster
• Alignment Length: 2077
• Identity: 432/2077 - (20%)
• Similarity: 744/2077 - (35%)
• Gaps: 736/2077 - (35%)

Human     9 ASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDREL----ASKKNPKL 69
            |:..||..|.|..|:|..||::.|||.|:.|:...|||       ...:|..|    ..||....
  Fly   226 ATFYSKSCFWWLTPLLWLGYKEPLELEDLGQMKLEDSA-------RSHYDHFLYIYTEKKKKSNS 283

Human    70 INALRRCFF---WR-FMFYGIFLYLGEVTKAVQPLLLGRII---------ASYDPDNKEERSIA- 120
            ..:|..|:.   |: |...||....|::...:.||.:.:|:         ||..|.......:|
  Fly   284 SPSLWYCYIKNSWQMFALGGILKLAGDLFALIGPLAIQKIVEYIEQLYAQASEPPAKSPGNEVAN 348

Human   121 -------------------------IY-------LGIGLCLLFIV------RTLLLHPAIFGLHH 147
                                     ||       |..|.|:.:||      :..|...:...|:.
  Fly   349 VLLSTSRILGTEFDEVFGTNIDKVRIYSSTWSDLLANGWCIAWIVLLAAITQGALSQASTHILNM 413

Human   148 IGMQMRIAMFSLIYKKTLKL-----------------SSRVLDK--------------------- 174
            .|::::.::..|||:|:|.|                 :|...|:
  Fly   414 TGIRIKTSLQGLIYRKSLLLNADGGCDSSDSAGQVQSTSSTSDEKQKNDDSMATPEHVDNPSEPN 478

Human   175 IS--IGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCG-LGFLIVLALF 236
            ||  ||.:.:.::.:.....|...:.|:.|..|.::|:::.|::..|..||..| :..::::...
  Fly   479 ISHDIGSITNHMTEDTRNIMEFFLIIHYAWAIPFKIAVVIYLLYMNLGISAVIGSIACIVIMTPL 543

Human   237 QAGLGRMMMKYRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAY 301
            |..:|..|.|..:..||...|||....:.:..|:.:|...|:|...|.|:..|:.|||
  Fly   544 QFFIGNAMSKNAEVIAGYTDERLKRIHDTLVGIKVIKLNAWDEVFLKKIQEARRKELK------- 601

Human   302 VRYFNSSAFFFSGFFVVFLSVLPYALIKGIILRKIFTTISFCIVLR---------------MAVT 351
              |.|..|.|::     .::||.:  |..:::  .|.|:...:.|.               :|:.
  Fly   602 --YLNKDATFWT-----LMAVLTH--IATVLI--TFVTLGVYVWLHRDQEFDLNASRLFSSLALF 655

Human   352 RQ-------FPWAVQTWYDSLGAINKIQDFLQKQE------------------------YKTLE- 384
            :|       ||..|.....:..:..:::.||:..|                        |:|.|
  Fly   656 QQLTVPLLIFPITVPIIIAARVSTRRLERFLKSSEIQKQFEGIRNMARILSKSDASLDMYETQEK 720

Human   385 YNLTTTEVVMEN-----------------------------------VTAFWEEGFGELFEKAKQ 414
            .|:|......||                                   .:...|.|..:|.::.::
  Fly   721 SNMTMRTAQAENRLNEKRLAQKSQTPELATNSTPLLQNAEESAEDISPSTVQELGHNKLVQQRRE 785

Human   415 NNNNR------------------------KTSNGD----DSLFFSNFSLLGTP--VLKDIN---- 445
            ...|.                        :..|.|    |||      ||..|  :...||
  Fly   786 LLRNTPYVAIRPPKMRGSVMERPVEFSVIRARNTDSWRRDSL------LLKMPDDIAVSINDGLF 844

Human   446 -----------------FKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKI--KHSGRISFC 491
                             ..:.:|:|..|.|..|:||||||..::.|:....|.:  ..:..||:.
  Fly   845 TWQPQSQMPVVQLHVPGIIVPKGKLTIVVGKNGSGKTSLLSALLMEMPLLAGNMFWHKTCTISYV 909

Human   492 SQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARI 556
            ||..|::..||:|||:||.|:...||..|::||.|:.||......|..::||.||.:|||||.||
  Fly   910 SQQPWLLNDTIRENILFGESFRPKRYDFVLEACALKPDIELMPRGDLSIIGERGINISGGQRQRI 974

Human   557 SLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLM--ANKTRILVTSKMEHLKKADKILIL 619
            ::|||:|..|::.::|.|...||....:.||:.|:.:::  :|:|.||||.::..:|:|:.::.:
  Fly   975 AIARAIYSSANVVIMDDPLASLDNEVGEHIFQHCIREMLQKSNRTFILVTQQLHRIKEAEYLIAI 1039

Human   620 HEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKK 684
            .:|.....|::::::.:||                             |.:.|.:|.::..:.|.
  Fly  1040 KDGRVEACGSYADIELMQP-----------------------------RITAEWNAIIAMAKAKN 1075

Human   685 QS----------------FKQTGEFGEKRKNSILNPIN------SIRKFSIVQKTPLQMNGIEED 727
            .:                .|...:.|.:|..|:....|      :|.....:....:|.|.:|||
  Fly  1076 DNPSQNPGEKTAGERWKLLKNVSKLGLQRSISVTMDANVACHADAIDGSGCISVANMQSNVVEED 1140

Human   728 SDEP--------------LERRLSL----------VP---DSEQGEAIL---------------- 749
            ....              |:|:.|.          ||   |..||:.::
  Fly  1141 DQVSVSYPIGNASCGGFNLQRKRSSIYGSRHLMYDVPLPIDECQGDDVIMRPRRRHTLGRRGSRN 1205

Human   750 ----PRISVIST-----------GPTLQARRRQSVL-----NLMTHSVNQG-------------- 780
                .|:|.:||           |..|.    :|||     :....||:.|
  Fly  1206 TNSSHRLSGLSTLTATSESSSISGDVLS----RSVLATSCSSYAESSVDGGDLATAAPEPRVQSW 1266

Human   781 ---QNIHRKTTASTRKVSLAPQANLTELDI---------------------------YSRRLSQE 815
               |::......| |..|..|...:...|:                           :.||:|..
  Fly  1267 QPPQHVTHHQPLS-RNASSPPAMEVANPDVKKSEEARRSNTSSESPLDDHVRGSFQQFLRRMSMR 1330

Human   816 TG----------------LEISEEI---------------------NEEDLKECF---------- 833
            ..                |.||||.                     :||..|:|.
  Fly  1331 RSNKPKNHHHPLSATNSILSISEESPPVVHFPASILATDGNKNETQSEEKPKKCVNIDSKETTIN 1395

Human   834 FDD--------------------------------------MESIP----AVTTW--------NT 848
            .||                                      .||:|    |:.|.        :.
  Fly  1396 CDDNCYSASDKELRANVTSSPADQEQHNERHVLAEVAGESGRESMPLARLAIDTERKYGKISDDI 1460

Human   849 YLRY--------ITVHKSLIFVLIW-CLVIFLAEVAASLVVLWLLGNTPLQDKGNSTHSR--NNS 902
            ||.|        ||:.  .|..||| ||.::        ..:||       .:.::.|.|  :..
  Fly  1461 YLMYIRAAGLPIITIF--FITALIWQCLRVY--------TDIWL-------QQWSNVHGRVASKG 1508

Human   903 YAVIITSTSSY---YVFYIYVGVADTLLAM-------GFFRGLPLVHTLITVSKILHHKMLHSVL 957
            :.|:..|...:   |.|.:|..::...:.|       |.:.|       ....:.||.|:|.::|
  Fly  1509 HVVLHPSEQDHEVTYYFRMYAAISCVCIIMALVSTPAGQYAG-------CNARRNLHDKLLQTIL 1566

Human   958 QAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPV 1022
            ...:........|.|:||||.|:|::|..:..|....:|..|:.:.||.:...:.|:|.|.|:|:
  Fly  1567 HKTLHFFQVTPLGRIVNRFSNDMAVIDKKIAATGQRLLQFTLLCLSAILINVTITPWILVLTLPI 1631

Human  1023 IVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTA 1087
            ..|:.:::.::..::::|:::|:...||:.:||..:::|:.|:|||.:|..|..:..|.|..:|.
  Fly  1632 CGAYYLIQKFYRCSARELQRIENATNSPVISHLSETIQGVTTIRAFNQQTRFTEILFKRLEANTI 1696

Human  1088 NWFLYLSTLRWFQMRIE-----MIFVIFFIAVTFISI-------------------------LTT 1122
            .:.|..::.||..:.::     ::||....|:|..|:                         :|.
  Fly  1697 AYALLNTSHRWLGVSLDYLGGCIVFVATVTALTAASVSCRRHYEATTSPSASASPSPFETYAVTK 1761

Human  1123 GEGEGR-----VGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRVFKF---------------- 1166
            ...|.|     ||:.:...:.:...|.|.|....|::....||.|:..:
  Fly  1762 SPSELRPSPSLVGLAINYTLLVPIYLNWVVKLLADMEMYAGSVERIAHYAQGQDADADADADADA 1826

Human  1167 -IDMPTEGKP------------------------------------------------------- 1175
             :|...:.:|
  Fly  1827 DVDADLDHEPSSNEDVSAEVDRSSQSDAGDKVYPGATTAAGDVDEDGDQQRIGGARGGGGDCGYR 1891

Human  1176 ------------------------------------------TKSTKPYKNGQL-------SKVM 1191
                                                      |..|...|:.||       .|.:
  Fly  1892 QGHENGAEANADKLNAGNVTGDGNHLNFHHPPATAGDKVEQATTKTSVIKDKQLPPQQDDKDKKV 1956

Human  1192 IIENSHVKKDD-------IWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSG 1249
            ::.|...:|.:       .||..|.:...:::.:|......::.|::..|..|||:|:.||||||
  Fly  1957 VLPNEPARKLERYQSVPISWPQRGDIHFDNVSLRYEGQKQNVISNLTLKIPAGQRIGICGRTGSG 2021

Human  1250 KSTL-LSAFLRLLNTEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQ 1313
            ||:| ||.|..|..|.|.|.||.|....|...:.|....:|||.|.:|:.|.|:||||:..:.|.
  Fly  2022 KSSLGLSLFGVLQTTRGHIYIDDVDIQRIRPDELRTRLSIIPQDVHLFNATIRENLDPHGYFQDL 2086

Human  1314 EIWKVADEVGLRSVIE-QFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLD 1377
            ::|...:...|:..:. ..|..||.|:.|||..||.||:||:||||::|..:..|:|||.::.||
  Fly  2087 QLWNCLELAQLKEFVNGHLPLGLDTVICDGGLNLSAGHRQLLCLARAILRGSVCLVLDEATSVLD 2151

Human  1378 PVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLN-ERSLFR 1438
            ..|...:.:....||...|:|...||:..:|:..:.:|:::.::.:..:.::|.. |.|:||
  Fly  2152 SSTESALLKAADLAFRGRTIITIAHRLTTILDYDRLIVLDQGRIVEDGNPRELQQLEGSVFR 2213

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	432/2077 (21%)
		Disordered R region. /evidence=ECO:0000269|PubMed:10792060	654..831		51/342 (15%)
		Interaction with GORASP2. /evidence=ECO:0000269|PubMed:21884936	1386..1480		13/54 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1452..1480
		PDZ-binding. /evidence=ECO:0000269|PubMed:11707463, ECO:0000269|PubMed:16331976	1478..1480
Sur	NP_001334732.1	ABC_6TM_SUR1_D1_like	301..682	CDD:350035	76/398 (19%)
		TM helix 1	301..324	CDD:350035	5/22 (23%)
		TM helix 2	385..433	CDD:350035	12/47 (26%)
		TM helix 3	480..525	CDD:350035	9/44 (20%)
		TM helix 4	526..575	CDD:350035	12/48 (25%)
		TM helix 5	587..631	CDD:350035	15/61 (25%)
		TM helix 6	648..681	CDD:350035	5/32 (16%)
		ABCC_MRP_domain1	837..1043	CDD:213217	68/205 (33%)
		ABC_6TM_SUR1_D2_like	1475..1811	CDD:350046	79/359 (22%)
		TM helix 1	1475..1496	CDD:350046	8/37 (22%)
		TM helix 2	1520..1568	CDD:350046	11/54 (20%)
		TM helix 3	1575..1620	CDD:350046	14/44 (32%)
		TM helix 4	1621..1670	CDD:350046	12/48 (25%)
		TM helix 5	1682..1727	CDD:350046	9/44 (20%)
		TM helix 6	1777..1810	CDD:350046	7/32 (22%)
		ABCC_MRP_domain2	1980..2201	CDD:213211	76/220 (35%)