Barcode: 005T9 Comments: This plate is an example of Variation/IQR detecting hits that don't show up when Z-score is used. Citations: This data was used for Figure 8a and 8b of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Firefly (target) readings in DasGupta R Kaykas A, Moon RT, Perrimon N " Functional genomic analysis of the Wnt-Wingless Signaling Pathway" Science. 2005 Apr 7 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 6850 9985 16729 13489 9016 16433 13814 11910 15353 13562 13953 17725 10138 9780 9420 16252 8176 14149 16661 12696 9923 12605 19059 15701 B 1657 696 17920 18499 14640 15744 22556 13635 7500 20824 8713 18040 13328 15776 12497 8027 11432 12402 10277 20683 13376 16521 18422 21117 C 24052 17091 13602 40768 16032 16486 13898 14756 12768 16803 23730 18721 16365 16816 42266 17008 13357 12102 14055 18234 9452 16730 10272 19313 D 9787 12560 14022 18489 11413 15370 14505 16520 7536 16619 11785 17335 23488 15227 16103 11515 17133 15376 7848 14437 17408 25696 8935 16256 E 14183 10817 18706 13034 16325 14523 16745 19920 11024 14245 7081 16021 8554 19248 9792 16229 17974 11332 14464 14116 11719 15696 12289 14227 F 14576 13829 13142 11725 7517 13884 13339 19832 14748 24803 8245 10724 7275 16857 12012 18028 6049 12444 13875 12004 9140 20729 16929 11490 G 5208 10272 9848 8616 8870 10490 9190 13336 9773 9632 9072 8485 11491 14322 11092 11116 10545 8711 12064 10983 13164 16024 8392 12533 H 22708 17754 9223 10313 7318 12738 12444 13392 9861 14309 8896 17552 10128 13865 7520 11764 9409 17417 15573 17962 7834 13536 10832 12744 I 11286 17952 15416 17600 14476 18347 12154 17912 12424 17234 13962 13344 19446 20693 10571 7708 10548 20563 9258 19068 15456 17938 11099 19995 J 19960 14524 19869 19144 15286 19752 14748 16864 12633 14333 11136 12528 53760 17190 13752 15280 10992 24484 12144 17204 16422 11611 8390 18804 K 30471 12652 14393 17547 24486 15115 14039 19296 19962 13451 14087 12294 8788 16611 12920 15160 9066 12578 10040 18577 12608 21282 12202 13323 L 13580 14563 8467 12394 17794 15120 12327 19656 11889 13719 11636 14256 9402 15436 10840 15717 9360 19971 10242 14432 8957 14250 16747 18535 M 18061 15323 15020 21781 17702 20455 12246 22231 10834 17181 8180 16584 10466 16627 13963 18217 7878 12925 15659 19434 12329 11205 15393 15458 N 14229 17364 12205 18039 13418 13803 7593 13936 19482 14672 10435 16875 14393 15335 19004 12128 12139 12001 15802 17523 13072 13408 19472 14570 O 10539 17699 13904 26442 16100 24496 14967 13555 11581 14005 15108 15451 15445 21920 13281 12737 12496 17584 18213 20032 15082 17692 13446 24145 P 12929 21844 9360 26288 10429 20858 15702 13692 18305 18141 13977 20097 17305 15413 11975 14454 13793 19760 20896 13425 13688 13248 14712 18679 Barcode: 017T8 Comments: This plate is used as an example of reasonably statistically normal data. Compare the S-W and J-B scores and Q-Q plot of this plate with plate 055B4, below. Citations: This data was used for Figure 7a of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Firefly (target) readings in DasGupta R Kaykas A, Moon RT, Perrimon N " Functional genomic analysis of the Wnt-Wingless Signaling Pathway" Science. 2005 Apr 7 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 4564 4137 5451 5235 6269 6986 6374 5592 7200 5797 5383 5968 8828 7556 7104 5815 6369 3794 4681 3787 5428 8688 9810 7506 B 567 524 4422 5954 6868 5483 6219 5751 8938 5344 8164 7001 7543 5188 6778 6453 9693 9445 7970 7317 9520 7179 9014 7264 C 7447 5878 5936 4976 8757 5830 7195 10146 9906 8295 6368 7109 6106 8887 8141 7040 7388 5178 6601 5469 5333 6332 6549 4488 D 5757 7088 5617 4364 6249 7928 5572 6896 6416 7195 5209 4457 5050 7244 5812 5920 8486 6809 6066 7108 4490 5928 9410 14236 E 6400 7591 8120 6579 6710 6960 8907 7195 10486 6160 9664 6630 10499 6274 5141 9249 8528 8625 7296 7853 4213 4906 9328 11321 F 9728 9179 8676 7212 7664 4484 4443 6067 7650 3346 12263 7235 7618 7443 5680 3956 10506 3892 11232 9104 11179 10297 7079 5558 G 8487 7259 6824 7981 9458 8229 6544 8004 10876 6650 6832 6776 6579 8833 8039 7004 7569 6146 7084 6393 7351 7607 7642 8353 H 9941 6810 5260 9296 9125 7868 5923 5440 5773 2921 5114 7202 7460 8400 6022 7530 6812 6140 5940 6758 5900 7644 6947 6756 I 8135 10922 8426 10325 8836 6930 7276 5792 6432 10226 7734 10745 9857 4464 8237 10305 6138 6795 4918 9896 6400 6630 5609 9821 J 6948 8053 8933 9176 9561 6858 6851 4652 5745 7394 8416 7324 9335 10608 9437 10236 4987 5506 7387 9027 6059 9281 9872 8464 K 10292 8185 7162 7041 6930 5713 7738 10656 11024 8019 10034 5968 9819 6144 7177 6464 5708 8304 10049 10154 6307 6562 8333 10201 L 6583 4849 6920 7584 4585 7008 6562 8618 6896 8537 6861 7050 9830 6769 7958 6344 6858 13984 9664 10724 9526 8547 9573 7715 M 10183 6152 5185 4747 6956 5048 6649 6745 10274 10069 10987 7329 8626 7075 10273 6396 8762 5783 6888 6905 8182 7792 7508 6101 N 6682 8358 6778 10077 9912 7875 4426 6865 6860 10253 4873 7223 8144 3824 10380 6263 7752 7003 7723 9738 10492 8166 10246 11569 O 9097 9402 6577 5547 6481 7677 9099 9592 6016 8072 9971 7096 9747 8858 5848 5156 9977 6619 10835 8275 9155 11376 11320 7536 P 13744 5932 4493 6000 6691 8149 5761 6759 8064 11077 5588 4407 6180 8396 7481 6456 8669 7777 5190 7453 6192 8928 7756 11177 Barcode: 018T3 Comments: This plate is an example of Z-score detecting hits that don't show up when Variation/IQR is used. Citations: This data was used for Figure 8c and 8d of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Firefly (target) readings in DasGupta R Kaykas A, Moon RT, Perrimon N " Functional genomic analysis of the Wnt-Wingless Signaling Pathway" Science. 2005 Apr 7 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 1977 1988 2914 4226 3771 3360 2380 2496 1632 3113 4267 2106 3256 2512 1643 1992 1831 3261 1556 2466 1827 4851 2952 4659 B 104 567 3513 3529 4332 1959 3227 2296 4560 2864 2648 2763 3383 2907 3172 2807 1770 2164 2845 3177 2731 5232 3479 2715 C 1529 3904 4103 3049 2709 2377 3382 2632 1834 2465 2941 2714 2544 2802 3424 2928 2535 1623 3288 3172 3077 2866 2445 1520 D 3648 3253 3418 2328 4822 3440 3264 2539 2547 3249 2824 3002 3577 2459 2602 2666 3648 2166 5013 4176 4040 4311 5296 2861 E 2354 4005 3200 2256 3008 3608 3257 3274 3378 2672 2129 3360 1968 2427 3974 4152 2768 2784 2216 4747 2561 2437 3929 1520 F 4487 3568 3216 3268 2305 3682 2944 3876 2048 1063 4109 1459 2609 2250 2517 1603 3592 2208 2006 6970 2539 2851 4299 3282 G 2400 3176 2629 3804 1804 2198 3030 3212 2365 3857 2836 934 1837 2075 1584 2330 2310 2036 2104 2889 2005 3490 2402 3837 H 1981 3226 2736 2593 2761 2819 3600 2952 2304 3826 2792 3820 2352 3184 2849 2813 1453 2363 3168 1917 2453 3036 4170 3712 I 2893 2679 2453 1744 2685 3238 2242 3514 3011 2215 2242 4992 2272 5083 2859 1851 1597 1906 2416 4097 2584 3929 2009 3883 J 4043 2918 3174 2864 3520 3816 2560 2720 2204 2333 1974 2904 3072 3144 2432 4500 3546 2645 2957 3817 2498 1561 3425 1493 K 3811 3760 4123 2833 2853 2816 2167 5143 3364 2784 3521 2408 3307 3046 2982 2263 2224 3206 2313 2596 1859 2280 4404 2761 L 2896 2853 3456 2429 2909 3556 4147 3213 2544 3942 2667 2368 2821 3322 3241 2314 3131 1396 2433 1989 3371 2984 3628 4262 M 3256 3206 3798 2660 3376 4275 2482 3385 2022 2272 3049 2132 4048 2802 4632 2985 3149 3062 1675 3481 2187 3482 2265 4073 N 4176 3024 4352 2069 4450 3925 3276 2618 2323 1955 2698 1987 2145 2284 3660 3491 3696 2185 3077 2650 3781 1863 3185 2125 O 4800 2458 3452 5152 3589 2099 2583 4113 2918 2663 3417 3536 3212 2522 3360 1857 3072 2637 3104 2536 2572 2042 2570 5898 P 4608 2333 4950 2678 3920 4521 4171 5084 6000 2592 4369 3648 3600 3833 3456 2548 3531 4112 2801 6823 4003 2859 5936 4578 Barcode: 024B4 Comments: This plate is used as an example of a narrow or single-row edge effect, where the (in this case) bottom row clearly has a different mean value than the plate as a whole. This is obscured somewhat in this case by the extreme values in P1 and P8, which may represent legitimate hits, but the edge effect is still detectable if those two values are masked. Citations: This data was used for Figure 6a of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Kc celltiter readings in Boutros M, Kiger A, et. al. "Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells" Science. 2004 Feb 6;303(5659):832-5 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 1406358 1677641 1674197 1646193 1586728 1666645 1660313 1689301 1655521 1695021 1663597 1678013 1709461 1696921 1734966 1202559 1532099 1519987 1497811 1543779 1529135 1669997 1553919 1532459 B 1715390 261003 1581060 1460118 1514643 1451230 1565888 1496047 1578664 1605092 1511207 1540243 1475134 1562104 1565972 1573308 1429702 1466794 1459286 1275428 1467866 1508795 1536087 1564128 C 1695177 1636413 1554935 1526655 1519219 1409886 1450102 1570004 1541807 1517815 1507715 1413138 1451802 1365969 1479855 1464854 1358365 1248096 1353217 1461166 1478463 1454358 1535323 1385457 D 1785846 1559664 1572068 1560856 1473194 1425502 1304380 1374993 1414958 1392325 1382877 1333113 1410246 1287416 1264144 1306068 1093710 1191183 1314808 1443614 1443730 1515875 1495015 1440282 E 1671957 1529663 1419634 1401022 1360241 1317016 1329181 1325089 1339525 1353873 1323689 1347485 1280748 1262192 1319028 1439154 1346709 1350397 1386157 1292416 1395449 1488979 1401494 1409366 F 1632928 1540975 1438786 1415634 1397653 1272424 1440374 1400338 1456874 1244336 1409046 1280424 1366461 1352349 1353517 1370097 1262496 1289380 1351217 1430682 1292856 1410410 1545159 1537147 G 1623860 1401170 1385385 1482335 1318332 1336529 1348113 1325457 1430210 1414098 1370993 1385685 1377829 1366893 1397421 1284792 1300080 1202907 1275932 1298064 1425974 1345785 1508883 1516263 H 1497471 1403602 1386401 1314128 1371473 1342257 936104 1395985 1294928 1394449 1336849 1335825 1350521 1279824 1232707 1280372 1215863 1174991 1375137 1302812 1284776 1273744 1324857 1428434 I 1661461 1341697 1430994 1325497 1300548 1115662 1321841 1267588 1442738 1352301 1404646 1266020 1230231 1210007 1441662 1394217 1313900 1356625 1173903 1252308 1402430 1270904 1388897 1435190 J 1582680 1478547 1426178 1363109 1443206 1130094 1353489 1331841 1290672 1374933 1588968 1170935 1224143 1343261 1295568 1307472 1219919 1204927 1240847 1410362 1276324 1407634 1524371 1656769 K 1682261 1564144 1242563 1388445 1319040 1299564 1373101 1256984 1337281 1368389 1435346 1251436 1231851 1405790 1350977 1299264 1241679 1260048 1302888 1470482 1403938 1362561 1476118 1479231 L 1640345 1489451 1433658 1381169 1305168 1318192 1226711 1334417 1367801 1397541 1405074 1344749 1375541 1350873 1348597 1218915 1247144 1177523 1247288 1332497 1383229 1401586 1479479 1544055 M 1669333 1673909 1507511 1379245 1332245 1332989 1375033 1334161 1366437 1348917 1319016 1327957 1522175 1292024 1339345 1289980 1301264 1236439 1270012 1413914 1580744 1461678 1595696 1582100 N 1642173 1562872 1520963 1355901 1363049 1348509 1357417 1376425 1406590 1398549 1416466 1397713 1310244 1347541 1337285 1296572 1340289 1305580 1448234 1486847 1413522 1624748 1601632 1640985 O 1706389 1514743 1476854 1332137 1400110 1367813 1378205 1418754 1438034 1486675 1519683 1386461 1454638 1384233 1476790 1547395 1411142 1332673 1490083 1529063 1569364 1587588 1482495 1607108 P 2025326 1759734 1804599 1723542 1687129 1713646 1684713 488466 1715870 1773890 1738718 1714038 1675141 1710493 1657273 1637633 1458786 1662245 1675465 1710805 1640321 1772594 1761002 1709889 Barcode: 051B3 Comments: This plate is used as an example of a broad edge effect, where the mean well value clearly varies with distance from the center. This type of distortion is harder to detect than single row/column effects, but can be corrected for if it is a systematic problem by normalizing against the mean or median value for the well location across a series of plates. Citations: This data was used for Figure 6b of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Kc celltiter readings in Boutros M, Kiger A, et. al. "Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells" Science. 2004 Feb 6;303(5659):832-5 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 1400742 1593084 1666017 1671593 1639293 1600388 1692117 1723562 1633504 1579648 1642965 1581524 1234651 1582236 1528603 1688565 1718110 1639257 1609256 1674557 1706313 1697853 1782198 1731198 B 1683765 224022 1590784 1519443 1493311 1477074 1449334 1562968 1546335 1468826 1519043 1370977 1518679 1468838 1531947 1510163 1490415 1481939 1480071 1518495 1564996 1526883 1576412 1696229 C 1730134 1610744 1598408 1553411 1506727 1466814 1467798 1401766 1531755 1540987 1579364 1388693 1462742 1495403 1377729 1530543 1418962 1465794 1494887 1526443 1601464 1567148 1643821 1701077 D 1681117 1650221 1557828 1539895 1482515 1424806 1400042 1360945 1461038 1435822 1322585 1432054 1413714 1467398 1324385 1467762 1231195 1451234 1493631 1537627 1496179 1640685 1689109 1701421 E 1517155 1661917 1565916 1543155 1443282 1371701 1208227 1308688 1397689 1284216 1368881 1348153 1281732 1412982 1223951 1398525 1337425 1266448 1461346 1572648 1545563 1648549 1688353 1656645 F 1657309 1549767 1543443 1537459 1477634 1381073 1223839 1392633 1367677 1353717 1432946 1398173 1370073 1402410 1210831 1278176 1313296 1253680 1440342 1545075 1526959 1589180 1607140 1627572 G 1472594 1568852 1528623 1519147 1488543 1271956 1452646 1276964 1406242 1458426 1233855 1377317 1278360 1271888 1402362 954252 1197583 1400506 1357225 1506259 1623588 1642709 1607020 1594156 H 1559708 1619988 1562532 1483171 1504231 1368997 1412370 1398817 1403366 1440382 1272464 1189695 1263932 1195647 1345361 1305040 1395985 1385697 1382001 1303784 1499731 1531887 1408850 1474406 I 1494967 1534931 1605524 1594072 1466514 1324825 1321116 1349009 1366525 1230687 1282792 1222559 1231267 1273600 1240051 1417938 1354841 1334417 1323065 1394329 1380577 1582496 1427362 1650873 J 1514299 1620728 1552163 1575892 1461682 1341269 1300312 1318560 1401822 1354153 1400626 1277096 1370449 1301472 1339825 1311448 1365097 1355013 1291264 1438378 1536047 1559736 1593556 1821035 K 1516691 1424802 1593156 1604128 1538387 1367961 1301872 1282776 1264516 1365969 1299984 1334801 1266064 1374145 1272608 1290204 1355165 1269240 1198959 1509479 1471662 1391349 1548251 1711449 L 1808763 1462934 1505631 1583664 1583096 1508603 1260656 1333985 1319728 1297684 1319044 1355601 1411242 1247504 1312336 1174103 1403046 1323129 1496379 1551111 1557544 1349329 1634821 1512531 M 1485435 1490987 1391737 1579356 1562744 1497119 1376037 1369285 1339109 1233563 1325561 1460590 1346917 1462738 1355253 1289852 1458674 1503811 1454050 1647897 1720626 1715950 1637013 1526227 N 1453866 1389757 1642133 1599956 1563312 1567004 1555804 1582124 1467474 1521407 1431514 1384533 1327057 1527059 1706525 1601272 1586964 1617192 1538171 1637077 1624700 1724254 1493075 1632996 O 1515375 1686421 1576000 1475414 1652977 1537027 1588804 1543771 1476634 1530675 1595704 1468346 1462018 1571500 1514903 1564832 1635337 1537855 1547711 1575656 1595748 1011596 1399205 1769534 P 1767298 1825255 1420778 1661813 1589664 1631940 1624804 1742282 1630880 1620128 1487291 1792519 1651157 1628220 1531803 1633300 1637525 1698645 1643125 1691913 1718330 1698285 1749206 1800523 Barcode: 055B4 Comments: This is an example of a plate whose data does not conform well to a normal curve. Compare the S-W and J-B scores and Q-Q plot of this plate with plate 17T8, above. Citations: This data was used for Figure 7b of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Kc celltiter readings in Boutros M, Kiger A, et. al. "Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells" Science. 2004 Feb 6;303(5659):832-5 Calculation: None - Showing raw 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 1271468 1581024 1595612 1575744 1580500 1604080 1612312 1620536 1638997 1659765 1611764 1642249 1640857 1660249 1573588 1664829 1561852 1568508 1695557 1640165 1536851 1712306 1645589 1701681 B 1559584 167298 1526495 1490411 1510487 1482175 1490215 1529239 1509787 1536823 1521759 1433278 1435370 1437534 1530711 1535807 1471974 1517083 1568836 1500647 1626456 1623264 1687369 1629076 C 1604788 1385169 1502419 1495587 1485319 1492647 1435346 1506611 1526811 1521683 1518915 1444530 1483839 1471358 1566656 1462866 1524691 1420478 1466282 1475762 1389881 1570900 1595796 1576816 D 1370561 1501683 1431686 1426734 1352153 1409938 1333649 1452546 1408106 1469914 1308344 1377145 1409682 1425286 1433758 1410854 1358101 1339345 1438942 1507955 1529671 1421538 1608532 1646893 E 1424998 1299096 1335121 1390677 1246052 1333541 1226011 1357565 1211663 1332793 1372033 1350721 1342929 1330373 1425314 1339493 1362105 1264044 1436978 1397437 1493719 1550755 1642005 1470442 F 1298132 1329941 1209539 1215923 1213467 1227155 1195907 1258832 1235087 1264728 1352937 1284688 1236119 1345713 1322009 1230735 1288400 1313060 1269048 1387333 1429242 1526163 1505451 1679065 G 1348729 1328801 1231603 1226271 1212431 1294000 1212543 1246992 1401594 1299544 1274420 1274796 1228175 1266900 1380177 1322409 1340145 1166551 1305384 1414306 1451742 1430454 1562464 1433266 H 1330193 1248152 1230583 1197603 1220891 1212379 1196111 1304724 1272816 1276208 1224271 1133314 1145870 1203103 1178767 1183659 1261696 1289132 1290336 1294868 1389865 1360017 1308048 1448994 I 1404006 1227943 1216055 1237299 1233979 1227075 1192607 1354309 1318916 1306328 1301028 1159310 1183191 1204659 1248836 1224463 1282160 1203555 1269240 1227235 1364269 1272284 1352153 1542451 J 1316800 1269112 1234511 1201079 1225643 1187691 1181327 1204347 1205775 1294676 1200187 1194295 1180143 1166635 1340501 1204243 1254564 1172711 1208555 1376801 1352925 1457618 1463234 1469242 K 1403734 1314108 1267332 1250488 1224179 1236559 1219259 1229071 1239155 1245176 1264172 1270992 1203727 1277840 1210563 1194583 1199503 1188267 1206579 1264940 1442674 1383465 1360185 1380029 L 1396673 1264480 1255652 1226151 1242063 1208387 1178063 1200123 1202075 1228623 1202979 1176979 1156302 1187343 1147158 1142874 1141198 1143310 1251092 1325273 1406782 1454102 1319056 1461306 M 1403314 1357277 1261024 1275876 1213135 1199607 1194427 1207651 1217891 1224803 1224019 1197351 1221691 1218283 1192259 1220087 1207567 1227011 1268316 1435954 1498963 1556512 1581872 1422482 N 1351453 1251824 1236855 1189719 1206839 1201903 1195675 1232543 1201331 1368889 1300924 1278740 1246760 1220867 1215163 1223323 1204859 1180815 1337425 1383229 1341465 1541291 1391633 1492903 O 1412910 1276688 1258740 1245684 1313008 1245112 1235751 1302288 1234391 1348821 1354389 1301844 1286948 1341713 1337973 1359129 1315328 1330541 1359545 1328925 1412626 1509595 1304876 1519683 P 1375537 1487263 1473606 1482871 1619456 1581768 1516283 1513875 1497179 1560192 1535047 1539647 1552595 1491283 1635757 1515155 1518971 1480315 1343993 1509459 1383013 1498795 1464978 1692769 Barcode: 056B4 Comments: This plate is used as an example of a pipetting error that is easilly detected in a visual setting. We refer to the series of low values in odd rows of column 1. (This is also an edge effect by nature of its location.) This is an extreme case, which could be detected by other means, but it illustrates the class of problems. These plates are loaded from 4 interleaved 96-well plates, so the error pattern suggests a problem across all of column 1 of the first 96-well plate. Citations: This data was used for Figure 6c of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was taken from the Kc celltiter readings in Boutros M, Kiger A, et. al. "Genome-Wide RNAi Analysis of Growth and Viability in Drosophila Cells" Science. 2004 Feb 6;303(5659):832-5 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 381532 1728158 1719110 1660349 1627560 1604468 1626268 1687785 1587844 1696549 1647137 1657621 1662397 1697689 1625236 1630868 1616612 1659933 1651333 1662877 1577324 1657985 1620616 1777674 B 1822815 628264 1735042 1417234 1515739 1537043 1547527 1488487 1553043 1555627 1576536 1487791 1589164 1589684 1545439 1557116 1426410 1518251 1552319 1483695 1504595 1350085 1625760 1669449 C 307239 1840483 1583316 1546615 1563924 1525563 1537647 1546655 1522307 1604036 1553055 1506147 1474874 1492119 1543319 1570952 1556844 1403634 1553627 1532791 1567204 1524231 1655581 1707957 D 1352809 1562232 1577736 1529699 1539463 1522859 1004080 1490011 1475158 1523367 1447642 1481835 1502187 1479687 1519063 1521407 1292056 1480867 1488863 1504123 1520131 1649641 1082957 1571060 E 309827 1856599 1553895 1548371 1520111 1445726 1483423 1444038 1452834 1455226 1442902 1481283 1436398 1431550 1471578 1405746 1495487 1437570 1556388 1542915 1590040 1605348 1698837 1583444 F 1502515 1608960 1618836 1512759 1525715 1500179 1390645 1476762 1412922 1423782 325652 1391293 1461394 1452022 1467634 1538579 1428606 1431098 1516791 1495919 1590896 1600428 1619740 582435 G 313432 1794887 1551471 1529775 1471918 1312848 1367673 1409594 1395257 1419498 1468958 1338833 1386069 1424482 1419074 1420906 1431742 1364853 1485459 1538003 1568568 1629396 1661821 1627648 H 1601124 1436402 1390497 1414930 1343121 1350237 1258896 1312336 1395921 1344529 1352637 1237931 1235983 1308112 1340821 1408098 1340113 1411774 1446006 1497583 1319936 1627836 1571556 1625656 I 327492 1552427 1139550 1487187 1362613 1339345 1394129 1402362 1427774 1436130 1388193 1255196 1222495 1293984 1408890 1392601 1422398 1391549 1403562 1457278 1531575 1484779 1498663 1596884 J 1661821 1695897 1454746 1488451 1369533 255691 1312636 1263068 1397489 1366553 1371153 1298768 1204959 1300476 1585244 1440454 1593940 1396761 1432914 1538323 1566256 1651893 1663133 1593784 K 416837 1590756 1483119 1275492 1459282 1364445 1340345 1307856 1298064 1301856 1319332 1289104 1300668 1339813 1361681 1346321 1423334 1388805 1502167 1521491 1577532 1578408 1690553 1597588 L 1619732 1526071 1638589 1413202 1383729 1295700 1333905 581459 1324713 1200679 1300708 1220891 1312800 1187215 1323797 1333249 1343661 1516675 1571892 1554759 1501479 1724034 1467650 1600064 M 428969 1525943 1524603 1397397 1394005 1352665 1289916 1360793 1300648 1321284 1341821 1321745 1295776 1350437 1309112 1370677 1359581 1413086 1484819 1587540 1500287 1674057 1730134 1827503 N 1525855 1287060 1447650 1407702 1395481 1596160 1305192 1279416 1341297 1332913 1302524 1327445 1358813 1392849 1391761 1427674 1333357 1416194 1554771 1637041 1642825 1276200 1703665 1688821 O 484302 1400754 1379173 1307292 1293360 1326097 1281660 1340229 1309232 1339089 1288820 1258052 1252304 1291920 1323537 1386425 1354733 1388061 1529235 1476630 1571028 1617428 1590588 1752030 P 1648177 1407454 1360637 1565248 1692821 1490971 1481875 293903 1511235 83857 1610792 1346781 1323421 1709161 1886872 1734206 1776090 1757742 1668349 1733242 1721982 643980 1718742 1889388 Barcode: 075B3 Comments: This is an artificial example of a flat plate caused by an extreme outlier (F20) masking the variation in the rest of the plate. Masking that well (this can be done by replacing the value with "NaN" or some othe non-numeric string) reveals a statistically normal plate. Citations: This data was used for Figure 6d of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was created artificially using random values with a normal distribution, mean of 90000, and sigma (stdev) of 1000. Well F20 was instead loaded with 1.3 * the largest value generated up to that point (values were generated by row). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 87275 90440 88569 89120 89426 91951 88697 89297 89878 91171 89669 88474 90272 89882 90153 90320 89909 88698 90703 89224 89202 88918 88367 88140 B 89938 90383 90390 89123 92299 89564 89699 88972 90163 89828 90369 90910 90122 88850 89842 89664 91025 89188 89670 89653 89690 89310 90551 88699 C 91108 89899 89199 88244 90818 87828 90625 89633 88795 91225 89083 92038 90837 89337 91248 90464 88647 89862 88624 90179 90252 90789 89739 87900 D 89666 91368 90130 89388 90477 90658 90436 90839 90354 89950 89001 91703 90100 90103 87893 89629 91867 90134 90832 87976 90707 92298 91743 90382 E 91665 89066 88724 89547 90520 89331 91475 88024 89579 89919 90572 90009 91003 91060 91197 88573 90054 89440 89678 88587 90255 89721 90256 89571 F 92319 92726 88909 88717 89723 90158 91202 90190 91094 90400 90605 89744 89164 89661 88820 90358 89954 88723 89514 120543 88683 90272 90002 90760 G 90854 89892 90041 90176 89929 90796 89627 89196 90358 89620 90387 90002 91209 91437 89835 88136 90060 90698 90440 89382 88837 91140 89753 89482 H 91408 90727 90394 90991 91736 91466 86365 89061 89795 90100 87994 90014 89378 91522 90404 90445 89240 91508 90447 88305 91010 90090 88249 90659 I 89844 90777 90780 89764 89080 89708 88547 89118 89690 88645 91514 90100 91257 89937 90036 90721 88852 90928 90791 91060 90392 89107 89276 90594 J 89780 89765 88216 89140 89252 91108 88776 89298 89627 88852 89135 89233 90882 88681 90930 89008 90409 90895 91169 92009 89769 88988 89634 89775 K 89339 90357 89884 90548 89440 88564 89628 91049 88953 89940 90152 91283 89488 91742 89370 89567 90451 90181 90438 91445 89954 88461 89140 91711 L 91799 90410 90142 90417 88387 88950 91076 89448 90329 91092 92074 89818 90614 89474 91150 90354 89748 90765 90442 89405 88990 89338 89794 90724 M 90502 89117 88183 90469 91584 90804 88658 90854 89595 90170 89489 89381 89284 90995 92896 88740 90449 89882 92151 89818 90366 88574 89174 89504 N 89340 88914 90810 88270 90703 88822 89418 91473 89415 89655 89788 89986 88690 91612 91151 90134 89502 89712 90198 88682 90305 90501 91307 89414 O 90773 90781 88200 91313 92435 91538 89779 90192 88722 91066 89254 89515 89163 90467 88511 90037 91234 89542 88095 89534 89990 90406 89524 88174 P 90221 90088 90296 88709 89381 90945 90890 90104 90101 91067 88222 91453 90233 89508 89865 90876 90001 91255 89582 90237 90830 88037 88078 90059 Barcode: 076B8 Comments: This plate shows a variation on extreme outliers, referred to above as a "flat" plate, where there is too little variation in the experimental wells compared to the control wells A1, A2, B1 and B2. Citations: This data was used as part of Figure 6 of and early draft of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending, but was left out of the final manuscript Source: This plate was created artificially using random values with a normal distribution, mean of 98000, and sigma (stdev) of 700. The row A control wells used a mean of 155000 and sigma of 4000. The row B controls used a mean of 31000 and sigma of 4000. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 150673 154822 98090 97650 97676 97585 99024 99822 97280 99968 97874 97367 97665 98440 97584 98691 97471 97585 97879 98653 98293 98933 97823 97914 B 34475 31896 99312 99138 98464 98194 97993 96501 98061 96046 97526 97147 97169 98286 98488 97993 98387 98216 98406 98475 97921 98356 97525 97791 C 98786 97026 99284 97296 97652 98513 97825 97800 98400 97570 97110 98339 97469 97992 97731 97487 98665 97523 97933 97735 98468 98849 98208 98012 D 98811 99059 97725 98263 98528 99416 99955 98542 98912 97584 99176 99102 98322 98593 97973 98558 98425 97958 98953 99397 97974 97802 96583 97758 E 96887 96919 95954 98427 98279 98795 99054 98111 98697 97870 98188 98898 98007 97804 98783 97805 97573 98792 96416 97843 98049 96736 98178 98677 F 98003 99314 97258 98325 98787 96714 98466 98608 97426 98224 98313 97619 97885 96767 97696 97712 98074 97727 98653 98290 98720 98687 98279 98341 G 99061 97781 97768 98142 97736 98836 97596 97431 97608 98430 98894 98409 97618 98856 98923 98961 98879 98062 98432 97357 99761 98630 97458 97851 H 97137 98337 97785 99285 98685 98505 97050 97817 98376 97468 97814 98415 97340 98007 95928 97686 98500 97623 97912 97664 97393 98013 97321 97586 I 98000 98192 97691 97227 97414 98563 98442 97575 97445 96564 97122 98428 97704 98454 97394 98392 98778 96859 97887 97501 97421 97917 96633 98066 J 97528 97206 98286 98025 98029 97080 97019 98287 98193 96721 97964 98621 98306 97506 97754 97545 97092 98116 97405 98358 98571 97959 96689 98833 K 97512 98601 97655 96879 97925 97919 97713 96767 97812 98089 98252 97683 98424 99213 97849 96847 98580 98504 99153 98071 98858 97218 98345 98804 L 99539 99695 97650 98127 97590 98450 98222 98514 97303 98344 97646 98354 98540 98370 97340 97896 97023 99246 97667 99034 98680 97754 97684 98223 M 97510 97445 98628 98009 97833 98084 99012 97769 99467 97911 98337 97437 97990 97649 98332 98195 97588 97398 98862 97775 96880 98989 97584 97629 N 99113 98615 97597 98992 98308 99667 97259 98610 97602 98975 98334 98401 97851 98229 98706 97567 97440 99279 98112 98226 97170 97746 99510 97596 O 98036 98068 99095 97647 96706 97293 97856 98220 97343 97269 97173 98102 96684 98750 98504 98147 97630 98733 98100 99232 98581 98780 97143 98689 P 98632 97359 97064 97877 97548 97411 97945 96922 98528 96897 98721 98312 97499 99792 98539 98234 97217 96991 97445 98408 97586 98902 97187 97815 Barcode: 077B11 Comments: This plate illustrates a case where the wells originating from one of the four 96-well plates that served as source material for this 384-well plate is showing consistently lower values, suggesting that the material on that source plate was damaged or aliquoted incorrectly. Citations: This data was used for Figure 6e of Ramadan N., Flockhart I, Booker M, Perrimon N, Mathey-Prevot B "Design and implementation of high throughput RNAi screens in cultured Drosophila cells", publication pending Source: This plate was created artificially using random values with a normal distribution, mean of 88000 (quadrants 1, 3 and 4) or 71000 (quadrant 2), and sigma (stdev) of 3000. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A 83679 70637 82378 69351 88315 67192 86709 72111 82838 74424 90844 76488 90537 71950 82255 66599 86894 70476 88683 72253 87249 72090 89625 71914 B 89242 86831 89593 89693 84070 86657 88020 87283 85192 86309 88958 87240 79996 90796 93099 83391 87460 90230 80848 90061 83499 91722 89699 91787 C 88441 67767 88979 72834 92255 74215 82842 72931 84956 72857 86672 69676 89069 73304 87128 65513 93593 81779 87467 69752 84754 71962 87162 71137 D 85244 90712 89221 82437 87389 85525 83521 85700 87156 84891 88411 86112 86806 82404 89052 93776 93455 89238 86915 81557 88815 87369 87283 91759 E 92937 74186 87573 73737 91925 66507 85917 70809 87275 67036 88621 74558 86614 67147 90457 72518 88513 65122 96017 77909 87717 75033 86892 69778 F 86349 89182 90059 81160 91209 88639 90516 86713 86733 85605 83985 88407 91213 89751 84586 89923 96925 89697 87104 84799 83235 90336 80984 86092 G 90301 66704 88519 69921 80248 71694 89531 73933 89435 71869 91507 73660 88863 70424 86980 72689 87893 75660 89689 72356 87387 75057 89387 67925 H 89767 88974 88950 87357 93467 90127 91521 85830 88689 89140 90442 89945 85472 85512 84075 92498 91052 88897 86694 90395 87245 89752 86270 89593 I 89057 74457 87999 68350 88325 69983 90645 70820 88446 67978 90337 72775 82446 73879 88842 71138 83337 66390 88379 74174 89384 62898 88181 70315 J 90516 85209 87463 86671 85465 87466 92132 87906 87820 91350 89273 86580 86193 87633 87096 81298 88438 84079 85134 89029 97593 89005 87452 90345 K 87041 67795 96850 67329 87805 70878 87714 68023 86580 70716 82829 69271 92157 68592 88727 69467 82125 70332 86984 74963 85732 78604 86046 69470 L 85702 86021 91427 81885 84100 83935 91689 89280 89723 86237 87609 85851 88260 88741 88433 89667 83465 87831 87844 90178 82371 89174 89685 91891 M 92040 70851 89140 71127 90377 70417 95019 75477 84280 73977 85159 71736 91031 65866 91566 70256 89927 72341 89808 70776 88864 73464 88399 68717 N 93864 90864 90052 87297 91515 88845 94228 83668 83083 84386 94159 90555 87209 87813 89514 85505 89610 89424 89109 90394 90682 90718 91662 82672 O 90690 68485 88608 72895 83445 68182 91120 73994 86171 74101 89628 69327 86628 69525 87937 68287 89382 69989 86609 69830 89200 63322 86615 75292 P 82257 90581 87489 86452 85478 91218 85645 88349 85967 89064 86717 94765 83734 87010 87789 89275 86505 86455 87408 88438 83079 84867 86728 87314