The file celltiter_data.txt is a tab-seperated dump of all the data points from the CellTiter Assays. Each line represents a data point resulting from one experimental pass on one well. The columns are as follows:
| DRSC Amplicon | Our internal identifier for the amplicon. | |
|---|---|---|
| Assay | Which assay was performed in this pass. | |
| Raw Exp Result | Platereader result. | |
| Z-score | Z-score for that result based on plate. | |
| S Primer | One of the two amplicon primers. | |
| R Primer | One of the two amplicon primers. | |
| Target Gene | Name of gene originally targetted by amplicon primers. | |
| PCR Length | Length of PCR product. | |
| Conc. | Concentration in RNA plate well. | |
| Gel Eval | Evaluation of QC gel data. | |
| PCR Eval | Evaluation of original PCR QC. | |
| PCR T7 Eval | PCR QC after T7 promoters added. | |
| RNA Comment | Any comments associated with the RNA plate. | |
| Aliquot Comment | Each 384-well experimental plate is aliquoted from 4 96-well RNA plates. If anything happened during the aliquot process, it is noted here. |