RNAi Stock Validation & Phenotypes

P{TRiP.HMS02002}attP40

Gene Information TRiP Stock Information hide
Symbol:Gs1
CG:CG2718
FBgn: FBgn0001142
Source: TRiP Line ID: HMS02002 Collection: RNAi
BDSC ID: 40836
Vector: VALIUM20 Insertion Site: attP40
Validation Test Results hide
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: stem cell loss
  • Phenotype Antibody Info: a-Spectrin antobody is from DSHB, 3A9; mouse Vasa antibody is from Santa Cruz Biotechnology, d-260,
  • Phenotype: no eggs
  • Penetrance: 100%
  • Temperature (°C): 29
Driver:eya composite-GAL4
Driver BDSC: not annotated
Contributor(s): Jason Tennessen
Reference: supplemental table 2, Fletcher et al, 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Eye phenotype
  • Phenotype: eyes absent
  • Penetrance: 46% (total number=127)
  • Temperature (°C): 25
Driver:eya composite-GAL4
Driver BDSC: not annotated
Contributor(s): Jason Tennessen
Reference: supplemental table 3, Fletcher et al, 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Eye phenotype
  • Phenotype: no eye
  • Penetrance: 16% (0% of 4 male; 20% of 20 female)
  • Temperature (°C): 25
Driver:GAL4-dpp.blk1
Driver BDSC: 1553
Contributor(s): Brian Calvi
Reference: Rotelli MD, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype: lethal before adulthood
  • Temperature (°C): 25
Driver:c587-gal4; nos-gal4
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Ovary phenotype
  • Phenotype: larval or pupal lethal
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: the average mitochondrial length was shorter than that in the wild type (L < 2 um, P < 0.05)
  • Penetrance: 61.25%
  • Temperature (°C): 25
Driver:Cg.PA
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype_3: GO0005739:mitochondrion | third instar larval stage, with Scer\GAL4[Cg.PA]
  • Phenotype_2: lethal - all die during pupal stage, with Scer\GAL4[Cg.PA]
  • Phenotype_1: size defective | third instar larval stage, with Scer\GAL4[Cg.PA]
  • Phenotype: embryonic/larval fat body | third instar larval stage, with Scer\GAL4[Cg.PA]
  • FlyBase Allele ID: FBal0279115
Driver:eya.enh1+E+2
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: supplemental table 2, Fletcher et al, 2019 PubMed Entry
Phenotype Results:
  • Phenotype_1: visible | adult stage, with Scer\GAL4[eya.enh1+E+2]
  • Phenotype: eye, with Scer\GAL4[eya.enh1+E+2]
  • FlyBase Allele ID: FBal0279115
Driver:Hml.?
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Gonzalez EA, et al., 2013 PubMed Entry
Phenotype Results:
  • Phenotype: immune response defective | adult stage, with Scer\GAL4[Hml.?]
  • FlyBase Allele ID: FBal0279115
Driver:da-GAL4
Driver BDSC: not annotated
Contributor(s): Patrick Jouandin, Norbert Perrimon
Phenotype Results:
  • Phenotype_1: larval lethal (L2/L3)
  • Phenotype: Developmental delay
  • Temperature (°C): 25?C
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Temperature*: °C
qPCR data: % Remaining after knockdown
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