RNAi Stock Validation & Phenotypes

P{TRiP.HMS01507}attP2

Gene Information TRiP Stock Information hide
Symbol:nej
CG:CG15319
FBgn: FBgn0261617
Source: TRiP Line ID: HMS01507 Collection: RNAi
BDSC ID: 37489
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:y w; Dmef2.R-Gal4
Driver BDSC: 27390
Contributor(s): Perrimon and Randklev, 2013, unpublished data.
Phenotype Results:
  • Phenotype: L2 lethal
  • Penetrance: 100%
  • Temperature (°C): 27
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: differentiation defect
  • Phenotype Antibody Info: a-Spectrin antobody is from DSHB, 3A9; mouse Vasa antibody is from Santa Cruz Biotechnology, d-260,
  • Phenotype: no eggs
  • Penetrance: 100%
  • Temperature (°C): 29
Driver:nubbin-Gal4
Driver BDSC: not annotated
Contributor(s): Raquel A. Oliveira
Reference: Silva RD, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: wing phenotype
  • Phenotype: Lethal when tested in combination with san RNAi
Driver:c587-gal4; nos-gal4
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Ovary phenotype
  • Phenotype: larval or pupal lethal
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: the average mitochondrial length was shorter than that in the wild type (L < 2 um, P < 0.05)
  • Penetrance: 61.25%
  • Temperature (°C): 25
Driver:SPARC-MI00329-GAL4
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Ke H, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype_2: GO0031012:extracellular matrix | third instar larval stage, with Scer\GAL4[SPARC-MI00329-GAL4]
  • Phenotype_1: exocytosis defective | third instar larval stage, with Scer\GAL4[SPARC-MI00329-GAL4]
  • Phenotype: embryonic/larval fat body | third instar larval stage, with Scer\GAL4[SPARC-MI00329-GAL4]
  • FlyBase Allele ID: FBal0263412
Driver:ppk.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Kwon MJ, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype_1: neuroanatomy defective | third instar larval stage, with Scer\GAL4[ppk.PU]
  • Phenotype: larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4[ppk.PU]
  • FlyBase Allele ID: FBal0263412
Driver:Pph13-GAL4
Driver BDSC: not annotated
Contributor(s): Andrew Zelhof
Phenotype Results:
  • Phenotype: The presence of a deep psuedopupil: Normal
  • Temperature (°C): 25
Driver:Mef2-GAL4
Driver BDSC: not annotated
Contributor(s): Fabio Demontis
Reference: Graca FA, et al., 2021 PubMed Entry
Phenotype Results:
  • Phenotype: Larval Phenotype: Normal
  • Temperature (°C): 25C
Driver:Mef2-GAL4
Driver BDSC: not annotated
Contributor(s): Fabio Demontis
Reference: Graca FA, et al., 2021 PubMed Entry
Phenotype Results:
  • Phenotype: Adult Phenotype: Normal
  • Temperature (°C): 25C
Driver:Pph13-GAL4
Driver BDSC: not annotated
Contributor(s): Andrew C Zelhof
Reference: Rylee J, et al., 2022 PubMed Entry
Phenotype Results:
  • Phenotype: Photoreceptor/Deep Pseudopupil (DPP) Phenotype: No Effect
  • Temperature (°C): 25C
Community Input (asterisk indicates required field) hide
Contributor*:
Driver*:
Driver BDSC:
Temperature*: °C
qPCR data: % Remaining after knockdown
Phenotype?
Contributor Email*: