RNAi Stock Validation & Phenotypes

P{TRiP.HMS00653}attP2

Gene Information TRiP Stock Information hide
Symbol:AP-2alpha
CG:CG4260
FBgn: FBgn0264855
Source: TRiP Line ID: HMS00653 Collection: RNAi
BDSC ID: 32866
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: F1 lethal
  • Temperature (°C): 29
Driver:Act5C-Gal4
Driver BDSC: not annotated
Contributor(s): Zeng et al., 2015
Reference: Zeng et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype: lethal
  • Temperature (°C): 25
Driver:GAL4-dpp.blk1
Driver BDSC: 1553
Contributor(s): Brian Calvi
Reference: Rotelli MD, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype: lethal before adulthood
  • Temperature (°C): 25
Driver:MTD-Gal4
Driver BDSC: not annotated
Contributor(s): Mariana F Wolfner
Reference: Zhang Z et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: germline phenotype
  • Phenotype: no hatching
Driver:nubbin-Gal4
Driver BDSC: not annotated
Contributor(s): Raquel A. Oliveira
Reference: Silva RD, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: wing phenotype
  • Phenotype: Lethal when tested in combination with san RNAi
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: the average mitochondrial length was shorter than that in the wild type (L < 2 um, P < 0.05)
  • Penetrance: 61.25%
  • Temperature (°C): 25
Driver:ey.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Choudhury SD, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_1: pupa, with Scer\GAL4[ey.PU]
  • Phenotype: head, with Scer\GAL4[ey.PU]
  • FlyBase Allele ID: FBal0257337
Driver:VP16.mat.?Tub67C
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Lee and Harris, 2013 PubMed Entry
Phenotype Results:
  • Phenotype_2: furrow canal, with Scer\GAL4[VP16.mat.?Tub67C]
  • Phenotype_1: embryo | embryonic stage 5, with Scer\GAL4[VP16.mat.?Tub67C]
  • Phenotype: embryo | embryonic stage 4, with Scer\GAL4[VP16.mat.?Tub67C]
  • FlyBase Allele ID: FBal0257337
Driver:nan
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Fang X, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_3: GO0005776:autophagosome
  • Phenotype_2: GO0005768:endosome
  • Phenotype_1: GO0005764:lysosome
  • Phenotype: embryonic/larval fat body
  • FlyBase Allele ID: FBal0257337
Driver:ppk.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Zong W, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype_3: GO0097447:dendritic tree | pupal stage P5, with Scer\GAL4[ppk.PU]
  • Phenotype_2: neuroanatomy defective | pupal stage P5, with Scer\GAL4[ppk.PU]
  • Phenotype_1: size defective | pupal stage P5, with Scer\GAL4[ppk.PU]
  • Phenotype: larval dorsal multidendritic neuron ddaC | pupal stage P5, with Scer\GAL4[ppk.PU]
  • FlyBase Allele ID: FBal0257337
Driver:SPARC-MI00329-GAL4
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Zang Y, et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype_2: melanotic mass phenotype, with Scer\GAL4[SPARC-MI00329-GAL4]
  • Phenotype_1: fat body, with Scer\GAL4[SPARC-MI00329-GAL4]
  • Phenotype: melanotic mass, with Scer\GAL4[SPARC-MI00329-GAL4]
  • FlyBase Allele ID: FBal0257337
Driver:elav-C155
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Choudhury SD, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_6: GO0008021:synaptic vesicle | larval stage, with Scer\GAL4[elav-C155]
  • Phenotype_5: lethal - all die before end of larval stage, with Scer\GAL4[elav-C155]
  • Phenotype_4: neuroanatomy defective | larval stage, with Scer\GAL4[elav-C155]
  • Phenotype_3: neurophysiology defective | larval stage, with Scer\GAL4[elav-C155]
  • Phenotype_2: locomotor behavior defective | third instar larval stage, with Scer\GAL4[elav-C155]
  • Phenotype_1: NMJ bouton | larval stage, with Scer\GAL4[elav-C155]
  • Phenotype: embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4[elav-C155]
  • FlyBase Allele ID: FBal0257337
Driver:Act5C.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Choudhury SD, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype: lethal, with Scer\GAL4[Act5C.PU]
  • FlyBase Allele ID: FBal0257337
Driver:Toll-6-D42
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Choudhury SD, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype_1: lethal - all die before end of larval stage, with Scer\GAL4[Toll-6-D42]
  • Phenotype: locomotor behavior defective | third instar larval stage, with Scer\GAL4[Toll-6-D42]
  • FlyBase Allele ID: FBal0257337
Driver:Mef2.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Choudhury SD, et al., 2016 PubMed Entry
Phenotype Results:
  • Phenotype: lethal - all die before end of pupal stage, with Scer\GAL4[Mef2.PU]
  • FlyBase Allele ID: FBal0257337
Driver:Pph13-GAL4
Driver BDSC: not annotated
Contributor(s): Andrew Zelhof
Phenotype Results:
  • Phenotype: The presence of a deep psuedopupil in retina: Normal
  • Temperature (°C): 25
Driver:HHLT-GAL4
Driver BDSC: not annotated
Contributor(s): Utpal Banerjee
Reference: Evans CJ, et al., 2021 PubMed Entry
Phenotype Results:
  • Phenotype: GFP fluorescence? lymph gland: Increased +
  • Temperature (°C): 29C
Driver:HHLT-GAL4
Driver BDSC: not annotated
Contributor(s): Utpal Banerjee
Reference: Evans CJ, et al., 2021 PubMed Entry
Phenotype Results:
  • Phenotype: GFP fluorescence? circulation: Increased ++
  • Temperature (°C): 29C
Driver:HHLT-GAL4
Driver BDSC: not annotated
Contributor(s): Utpal Banerjee
Reference: Evans CJ, et al., 2021 PubMed Entry
Phenotype Results:
  • Phenotype: GFP fluorescence ?heart tube: Decreased / missing
  • Temperature (°C): 29C
Driver:Pph13-GAL4
Driver BDSC: not annotated
Contributor(s): Andrew C Zelhof
Reference: Rylee J, et al., 2022 PubMed Entry
Phenotype Results:
  • Phenotype: Photoreceptor/Deep Pseudopupil (DPP) Phenotype: No Effect
  • Temperature (°C): 25C
Driver:ptc-Gal4; uas-Dcr-2
Driver BDSC: not annotated
Contributor(s): Jeff Axelrod and Song Song
Phenotype Results:
  • Phenotype Category: planar cell polarity in wing disc
  • Phenotype: polarity looks fine
  • Temperature (°C): 29c
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