RNAi Stock Validation & Phenotypes

P{TRiP.HMS00464}attP2

Gene Information TRiP Stock Information hide
Symbol:Ask1
CG:CG4720
FBgn: FBgn0014006
Source: TRiP Line ID: HMS00464 Collection: RNAi
BDSC ID: 32464
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:y w; Dmef2.R-Gal4
Driver BDSC: 27390
Contributor(s): Perrimon and Randklev, 2013, unpublished data.
Phenotype Results:
  • Phenotype: Larval lethal
  • Penetrance: 100%
  • Temperature (°C): 27
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: no phenotype, grows to adulthood
  • Temperature (°C): 29
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Richelle Sopko and TRiP, 2014
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • qPCR reference gene: tubulin
  • Phenotype Category: non-maternal effect
  • Female Fertility: 38% hatching
  • Temperature (°C): 29
  • qPCR % Transcript Remaining: 6.0%
Driver:Act5C-Gal4
Driver BDSC: not annotated
Contributor(s): Zeng et al., 2015
Reference: Zeng et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype: lethal
  • Temperature (°C): 25
Driver:MTD-Gal4
Driver BDSC: not annotated
Contributor(s): Mariana F Wolfner
Reference: Zhang Z et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: germline phenotype
  • Phenotype: reduced hatching
Driver:c587-gal4; nos-gal4
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Ovary phenotype
  • Phenotype: viable, un-hatch, reduced egg
Driver:c587-gal4; PZ1444
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Germ cell phenotype
  • Phenotype: weak tumorless ISCs
Driver:c587-gal4; Dad-LacZ
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Germ cell phenotype
  • Phenotype: weak tumor, moderate expansion
Driver:c587-gal4; tub-gal80ts
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Germ cell phenotype
  • Phenotype: weak tumor
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: No phenotype
  • Temperature (°C): 25
Driver:nos.PG, VP16.nos.UTR, VP16.otu
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: female semi-sterile, with Scer\GAL4[nos.PG], Scer\GAL4[VP16.nos.UTR], Scer\GAL4[VP16.otu]
  • FlyBase Allele ID: FBal0248219
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