RNAi Stock Validation & Phenotypes

P{TRiP.HMS00409}attP2

Gene Information TRiP Stock Information hide
Symbol:Pp1-87B
CG:CG5650
FBgn: FBgn0004103
Source: TRiP Line ID: HMS00409 Collection: RNAi
BDSC ID: 32414
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:y w; Dmef2.R-Gal4
Driver BDSC: 27390
Contributor(s): Perrimon and Randklev, 2013, unpublished data.
Phenotype Results:
  • Phenotype: Early pupal lethal
  • Penetrance: 100%
  • Temperature (°C): 27
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: agametic
  • Phenotype Antibody Info: a-Spectrin antobody is from DSHB, 3A9; mouse Vasa antibody is from Santa Cruz Biotechnology, d-260,
  • Phenotype: no eggs
  • Penetrance: 100%
  • Temperature (°C): 29
Driver:w; matalpha4-GAL-VP16
Driver BDSC: 7063
Contributor(s): Lynn Cooley and Kathleen Ayers, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Staining info: Alexa 488-phalloidin (Molecular Probes) and DAPI (Molecular Probes)
  • Penetrance: 100%
  • Ovary Phenotype: degeneration, various stages
  • Female Fertility: eggs laid, no progeny
  • Temperature (°C): 25
 Image Link
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Richelle Sopko and TRiP, 2014
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Description: no vitellogenic egg chambers
  • Phenotype Category: oogenesis defect
  • Phenotype: no egg
  • Female Fertility: no eggs
  • Temperature (°C): 29
Driver:c587-gal4; nos-gal4
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Ovary phenotype
  • Phenotype: larval or pupal lethal
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: No phenotype
  • Temperature (°C): 25
Driver:nos.PG, VP16.nos.UTR, VP16.otu
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Sopko R et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: female sterile, with Scer\GAL4[nos.PG], Scer\GAL4[VP16.nos.UTR], Scer\GAL4[VP16.otu]
  • FlyBase Allele ID: FBal0248170
Driver:hsFlp-act>stop>Gal4
Driver BDSC: not annotated
Contributor(s): Jeff Axelrod and Song Song
Phenotype Results:
  • Phenotype Category: planar cell polarity in wing disc
  • Phenotype: adult wing hair orientation defects - swirls
Driver:ptc-Gal4; uas-Dcr-2
Driver BDSC: not annotated
Contributor(s): Jeff Axelrod and Song Song
Phenotype Results:
  • Phenotype Category: planar cell polarity in wing disc
  • Phenotype: lethal
  • Temperature (°C): 29c
Driver:ci
Driver BDSC: not annotated
Contributor(s): Jeff Axelrod and Song Song
Phenotype Results:
  • Phenotype Category: planar cell polarity in wing disc
  • Phenotype: polarity looks fine
  • Temperature (°C): 29c
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