RNAi Stock Validation & Phenotypes

P{TRiP.HMS00308}attP2

Gene Information TRiP Stock Information hide
Symbol:dia
CG:CG1768
FBgn: FBgn0011202
Source: TRiP Line ID: HMS00308 Collection: RNAi
BDSC ID: 33424
Vector: VALIUM20 Insertion Site: attP2
Validation Test Results hide
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: agametic
  • Phenotype Antibody Info: a-Spectrin antobody is from DSHB, 3A9; mouse Vasa antibody is from Santa Cruz Biotechnology, d-260,
  • Phenotype: no eggs
  • Penetrance: 100%
  • Temperature (°C): 29
Driver:w; matalpha4-GAL-VP16
Driver BDSC: 7063
Contributor(s): Lynn Cooley and Kathleen Ayers, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Staining info: Alexa 488-phalloidin (Molecular Probes) and DAPI (Molecular Probes)
  • Penetrance: 100%
  • Ovary Phenotype: normal
  • Female Fertility: fertile
  • Temperature (°C): 25
Image Link
Driver:Act5C-Gal4
Driver BDSC: not annotated
Contributor(s): Zeng et al., 2015
Reference: Zeng et al., 2015 PubMed Entry
Phenotype Results:
  • Phenotype: lethal
  • Temperature (°C): 25
Driver:nubbin-Gal4
Driver BDSC: not annotated
Contributor(s): Raquel A. Oliveira
Reference: Silva RD, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: wing phenotype
  • Phenotype: Enhanced wing morphological defects with san RNAi
Driver:c587-gal4; nos-gal4
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Ovary phenotype
  • Phenotype: partial lethal, no egg
Driver:c587-gal4; PZ1444
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Germ cell phenotype
  • Phenotype: moderate tumorless ISCs
Driver:c587-gal4; Dad-LacZ
Driver BDSC: not annotated
Contributor(s): Jian-Quan Ni
Reference: Gao Y, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Germ cell phenotype
  • Phenotype: moderate tumor, moderate expansion
Driver:Cg-Gal4
Driver BDSC: not annotated
Contributor(s): Chao Tong
Reference: Zhou J, et al., 2019 PubMed Entry
Phenotype Results:
  • Phenotype Category: Mitochondria phenotype
  • Phenotype: No phenotype
  • Temperature (°C): 25
Driver:pnr.PU
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Couturier L, et al., 2017 PubMed Entry
Phenotype Results:
  • Phenotype_1: cell shape defective, with Scer\GAL4[pnr.PU]
  • Phenotype: external sensory organ precursor cell, with Scer\GAL4[pnr.PU]
  • FlyBase Allele ID: FBal0257201
Driver:VP16.mat.?Tub67C
Driver BDSC: not annotated
Contributor(s): FlyBase, re-organized by DRSC.
Reference: Abreu-Blanco MT, et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype: wound healing defective | embryonic stage, with Scer\GAL4[VP16.mat.?Tub67C]
  • FlyBase Allele ID: FBal0257201
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Temperature*: °C
qPCR data: % Remaining after knockdown
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