RNAi Stock Validation & Phenotypes

P{TRiP.GL00483}attP2

Gene Information TRiP Stock Information hide
Symbol:Ntf-2
CG:CG1740
FBgn: FBgn0031145
Source: TRiP Line ID: GL00483 Collection: RNAi
BDSC ID: 35636
Vector: VALIUM22 Insertion Site: attP2
Validation Test Results hide
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Dong Yan and Ralph Neumuller, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Phenotype Category: agametic
  • Phenotype Antibody Info: a-Spectrin antobody is from DSHB, 3A9; mouse Vasa antibody is from Santa Cruz Biotechnology, d-260,
  • Phenotype: no eggs
  • Penetrance: 100%
  • Temperature (°C): 29
Driver:w; matalpha4-GAL-VP16
Driver BDSC: 7063
Contributor(s): Lynn Cooley and Kathleen Ayers, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Staining info: Alexa 488-phalloidin (Molecular Probes) and DAPI (Molecular Probes)
  • Penetrance: 100%
  • Ovary Phenotype: early arrest, very small ovaries
  • Female Fertility: no eggs
  • Temperature (°C): 25
Image Link
Driver:MTD-Gal4
Driver BDSC: 31777
Contributor(s): Lynn Cooley and Kathleen Ayers, 2013
Reference: Yan D et al., 2014 PubMed Entry
Phenotype Results:
  • Staining info: Alexa 488-phalloidin (Molecular Probes) and DAPI (Molecular Probes)
  • Penetrance: 100%
  • Ovary Phenotype: agametic
  • Female Fertility: no eggs
  • Temperature (°C): 25
Image Link
Driver:nubbin-Gal4
Driver BDSC: not annotated
Contributor(s): Raquel A. Oliveira
Reference: Silva RD, et al., 2018 PubMed Entry
Phenotype Results:
  • Phenotype Category: wing phenotype
  • Phenotype: No phenotype
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qPCR data: % Remaining after knockdown
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