Reagents and Results:
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Cell Screening dsRNAs (4)
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Hits in 10 of 83 screens (Click title to view all hits in that screen)
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Cell Growth and Viability (pmid: 14764878 )
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VSV Screen
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S2R+ Morphology Screen (pmid: 21893601 )
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Hh Signalling (pmid: 16311596 )
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Genes involved in protein secretion and Golgi organization (pmid: 16452979 )
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Phagocytosis of Apoptotic Cells (pmid: 18984163 )
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p53
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Genes regulating lipid storage in Drosophila melanogaster
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Hedghog secretion (pmid: 22432040 )
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Comparison of GPCR Induced vs. Small G-Protein Induced Signalling to the Nucleus (pmid: 23177739 )
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC01375 |
Sequence: |
GCAACGGCCAGTGCATCGGCCAGCGGTACGGGATCGGCATCGGGAGCGGTAACATTCGCATCGGTTACGGCAGCAACAACAATAACGCCAGGACGTCGAAAACTGCGACGCAAGTTTCAGGGGCAACAAAACCGTAATTCCACCTTGGGCAGGAGGCGAAAACTGCGTTTGCATCCCTATCATCGGCGCAATGCAACACATGCCACACGCACCACGACTGTGGCAAGTGTCTCTGCGACGGCAACTACGCTGCTACCAAAGATGCCCCACAAGCAGCCCTTCAACTATCACACCCACCTGCACTCGAGCTTCCAGCAGCAGCAACAGCAGCAACATGTTGCCGGTGGCCAAGTTACCCAGCAGCAGCAGCAGCAGCAACAAGTGCATGTTGTTGCCGTAACACCGCGCCCGCAACAGCGGCAACAACAGCCCGGATTTGCCATAGTACCTGCAACCTACTATCACCAGCAGCAGCAGCAACAGCAACGAACACTTTTG |
| "R" Primer: |
GCAACGGCCAGTGCATC |
| "S" Primer: |
CAAAAGTGTTCGTTGCTGTT |
| Amplicon Length: |
498 |
| Target Gene(s): |
wb |
| Potential Off-Target Number: |
717 |
| CAR/CAN repeats: |
CAR Repeat |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0302734 |
| Exon 8 length |
1229 |
dsRNA Position Relative to Exon 8: 530 - 1027 |
| Transcript |
FBtr0302735 |
| Exon 10 length |
1042 |
dsRNA Position Relative to Exon 10: 343 - 840 |
| Transcript |
FBtr0302732 |
| Exon 9 length |
1103 |
dsRNA Position Relative to Exon 9: 404 - 901 |
| Transcript |
FBtr0302733 |
| Exon 9 length |
1103 |
dsRNA Position Relative to Exon 9: 404 - 901 |
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Historical Amplicon Information
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| HFA Amplicon ID: |
HFA01375 |
| Amplicon Nickname: (Old Oligo Name) |
AE002690_sanger_3716 |
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Click
here for amplicon info documentation
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14306478 |
| End: |
14310975 |
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Hits in 1 of 87 screens (Click title to view all hits in that screen)
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CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca2+ Entry (pmid: 16645049 )
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC03634 |
Sequence: |
GTACCCAGCTCAGACTACGAGTACATAGTGGTGGTTCAGATGGAGGGCAGCAGTTTCCATCGCGAAGACGCGGAGATCCAGCGCATGAATGATCTGAGCTTAGTGCCCAAATCTACGGGGAATGTTTCCATCGGAGCATATGGACAATTTTACCATCCGCTCTACTTCCAACTGCCTCCGCAGTTCTACGGAGATCGGACTAGTAGCTATGGTGGCTTTTTGTACTTCACCCTGATCACCGAAGGTGCCCACAAGCCCTTGGAGCGAAATATTTTGGGTCAGTATCCATTGGTTCAGTTACACGCGCACTCCAAGTTGCTACTGGACTTTTACGAGTACGAAGAGTTCGAGTACTCCTTGAACGTGACCCATCGGGTGCCGCTGCATGAGTCCTTCTGGAAGTACCACCACACCAGCCAGGCAGTGGATCGTAATACTCTAATGGCTGCCCTCCAAAACATTCGACACATCTTCATTCGAGCCTTTG |
| "R" Primer: |
GTACCCAGCTCAGACTACG |
| "S" Primer: |
CAAAGGCTCGAATGAAGATG |
| Amplicon Length: |
487 |
| Target Gene(s): |
wb |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0302732 |
| Exon 11 length |
1360 |
dsRNA Position Relative to Exon 11: 849 - 1335 |
| Transcript |
FBtr0302733 |
| Exon 11 length |
1360 |
dsRNA Position Relative to Exon 11: 849 - 1335 |
| Transcript |
FBtr0302734 |
| Exon 11 length |
1360 |
dsRNA Position Relative to Exon 11: 849 - 1335 |
| Transcript |
FBtr0302735 |
| Exon 11 length |
1360 |
dsRNA Position Relative to Exon 11: 849 - 1335 |
| Transcript |
FBtr0305063 |
| Exon 11 length |
1360 |
dsRNA Position Relative to Exon 11: 849 - 1335 |
| Transcript |
FBtr0302731 |
| Exon 11 length |
1360 |
dsRNA Position Relative to Exon 11: 849 - 1335 |
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Historical Amplicon Information
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| HFA Amplicon ID: |
HFA03634 |
| Amplicon Nickname: (Old Oligo Name) |
wb |
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Click
here for amplicon info documentation
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14318402 |
| End: |
14322888 |
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Hits in 1 of 54 screens (Click title to view all hits in that screen)
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ERK Signalling Dynamics (pmid: 22028469 )
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC23003 |
Sequence: |
CAACATCACCGAACCAGTGTAAATAGCAGTGTAAATATCAATATAAGCAGGCAATTCAATGAAATTATACAAACGGCAACGGCCAGTGCATCGGCCAGCGGTACGGGATCGGCATCGGGAGCGGTAACATTCGCATCGGTTACGGCAGCAACAACAATAACGCCAGGACGTCGAAAACTGCGACGCAAGTTTCAGGGGCAACAAAACCGTAATTCCACCTTGGGCAGGAGGCGAAAACTGCGTTTGCATCCCTATCATCGGCGCAATGCAACACATGCCACACGCACCACGACTGTGGCAAGTGTCTCTGCGACGGCAACTACGCTGCTACCAAAGATGCCCCACAAGCAGCCCTTCAACTATCACACCCACCTGCAC |
| "R" Primer: |
CAACATCACCGAACCAGTGT |
| "S" Primer: |
GTGCAGGTGGGTGTGATAGTT |
| Amplicon Length: |
378 |
| Target Gene(s): |
wb |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0302735 |
| Exon 10 length |
1042 |
dsRNA Position Relative to Exon 10: 268 - 645 |
| Transcript |
FBtr0302734 |
| Exon 8 length |
1229 |
dsRNA Position Relative to Exon 8: 455 - 832 |
| Transcript |
FBtr0302733 |
| Exon 9 length |
1103 |
dsRNA Position Relative to Exon 9: 329 - 706 |
| Transcript |
FBtr0302732 |
| Exon 9 length |
1103 |
dsRNA Position Relative to Exon 9: 329 - 706 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14306403 |
| End: |
14310780 |
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC39164 |
Sequence: |
GTCCGGAAGGTTATACGGGTGATCATTGTCAGGTGTGCGACGATGGGTACTTCGGGAATCCACGCCAGCCGGGAAGCAGTTGTCAGCGCTGCGATTGCGCCGGAGGGCCCTGCAACGTGACCACCGGGGAGTGCATCACGTGCCGCGGAAACACAGAGGGTTGGCACTGCGAGCGCTGCAAGCTGGGCTACTGGGGTGATCCGGCCGTGGGCTGTGATCCCTGTCATTGCCACACGGAAGGATCGGAGAGCGGACTATGCGACAGCACCGATGGTCAATGCCTGTGTAAGCCACGTTATGCTGGACAGAAGTGCGATGAATGTGACGTGGGCTATGCTAATGTTGAGCTACGCTGTCCATCCTGCAACTGCGATCCATTGGGCTCGTTGGTCCAGGACAGATGTGATCCCCACACTGGACAATGCCATTGTAAGGAGGGCGTTA |
| "R" Primer: |
GTCCGGAAGGTTATACGGGT |
| "S" Primer: |
TAACGCCCTCCTTACAATGG |
| Amplicon Length: |
444 |
| Target Gene(s): |
wb |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0305063 |
| Exon 4 length |
809 |
dsRNA Position Relative to Exon 4: 195 - 638 |
| Transcript |
FBtr0302731 |
| Exon 4 length |
809 |
dsRNA Position Relative to Exon 4: 195 - 638 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14286344 |
| End: |
14290787 |
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Cell Screen Follow-Up dsRNAs (2)
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC32401 |
Sequence: |
GTCAGCGTCTGACAAACGAACTGTCAAAATGTCAGACGCGTCAATAAAGCCACGAAAATTAAAAAAACATCTGCGGGCAGCAGATGTAAAGCCTCGTGGCATGATGACAAAAAGCCTGCTGCTGCATCTAT |
| "R" Primer: |
GTCAGCGTCTGACAAACGAA |
| "S" Primer: |
ATAGATGCAGCAGCAGGCTT |
| Amplicon Length: |
131 |
| Target Gene(s): |
wb |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0302732 |
| Exon 9 length |
1103 |
dsRNA Position Relative to Exon 9: 85 - 215 |
| Transcript |
FBtr0302733 |
| Exon 9 length |
1103 |
dsRNA Position Relative to Exon 9: 85 - 215 |
| Transcript |
FBtr0302735 |
| Exon 10 length |
1042 |
dsRNA Position Relative to Exon 10: 24 - 154 |
| Transcript |
FBtr0302734 |
| Exon 8 length |
1229 |
dsRNA Position Relative to Exon 8: 211 - 341 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14306159 |
| End: |
14310289 |
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC36402 |
Sequence: |
CTTCGTTGGGTTACTTGGGAAATCGCTTGACCAGCTATGGGTCGAGACTGCAGCTGGTGTTGTCGTGGGATGTGATAAGAGGCGATAGATCGGGAAAGCCCACGACGGGGCCCAATGTCATCCTAGTCGGCAAGAATGGACTGAAGATTGCCTTCGGCGATGAATCCTTGGACGGATTGGGAATCAACTTGAATGTAACCCTAACAGAGGTTGGCTGGTATCACGTGCCACCCACTGTCGTTGATATAAAAACAAGACTTCGGCGAACCGAGGGCGGTGACTATCACGGAGAATCTGTTACCCGTTCCCAGTTTCTCTCCGTTCTCG |
| "R" Primer: |
CTTCGTTGGGTTACTTGGGA |
| "S" Primer: |
CGAGAACGGAGAGAAACTGG |
| Amplicon Length: |
327 |
| Target Gene(s): |
wb |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0302731 |
| Exon 3 length |
1892 |
dsRNA Position Relative to Exon 3: 1244 - 1570 |
| Transcript |
FBtr0305063 |
| Exon 3 length |
1892 |
dsRNA Position Relative to Exon 3: 1244 - 1570 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14285199 |
| End: |
14289525 |
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Transgenic RNAi Project (TRiP) Fly Stocks (1)
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Long Hairpin JF03238
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General Hairpin Information
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| TRiP #: |
JF03238 |
BDSC Stock #: |
29559 |
| Gene ID: |
CG42677 |
Gene Name: |
wb |
| Vector: |
VALIUM10 |
Hairpin ID: |
TR02733P.1 |
| Classifications: |
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Sense Oligo: |
AAGAATTCCTTCGTTGGGTTACTTGGGA |
| Antisense Oligo: |
AGTCTAGATCGTAAATCCGCTTGAATCC |
| Insertion Location: |
attP2 |
Gene Information for
wb:
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| Symbol: |
wb |
Gene Name: |
wing blister |
| Flybase ID: |
FBgn0261563 |
Entrez Gene: |
43946 |
| Synonyms: |
A1, CK02229, CG15288, l(2)br1, BEST:CK02229, LanA1,2, fus5, lethal group A1, laminin, BG:DS03792.2, alpha laminin, alpha[[1,2]], CG32970, wing blistered, D-laminin alpha2, l34Fb, l(2)34Fb, SGP cluster fusion defects 5, lamininW, l(2)09437, CG42677, BG:DS03792.1, lethal 1 in the black-reduced region, Laminin alpha1,2, CT35236, DLAM, LM-A/alpha2, Laminin A2, &agr1,2Lan, laminin alpha[[1,2]], Laminin alpha1, 2, laminin alpha[[1, 2]], LanA1, alpha[[1, &agr1, 2Lan, wing blister, wingblister, Wb, LanWb, alpha1 |
| Old Flybase IDs: |
FBgn0004002, FBgn0025691, FBgn0032544, FBgn0052970, FBgn0028885 |
| NCBI Nucleotide Accs: |
CG15288, AA140696, AA140697, AA141179, AA141691, AA141692, AC092248, AE003409, AF135118, AQ025808, AQ026114, AQ026440, AQ026443, BH011482, CL844479, CZ467753, CZ468884, CZ472234, CZ473412, CZ473479, CZ477975, CZ478020, CZ480408, CZ486441, CZ486442, CZ486508, CZ486509, CZ488951, CZ488952, G00417 |
| NCBI Protein Accs: |
AAN10875, AAF44861, AAD31714, AAO41191, ACL83031, AAF44862, AAF53381, ADV37059, ADV37056, ADV37058, ADV37057 |
| UniProt Accs: |
Q8IP51, M9MRU0, R9PY40, Q9XZC9 |
| Gene Ontology IDs: |
GO:0005605, GO:0005604, GO:0008406, GO:0007502, GO:0007476 |
| Protein Domains: |
IPR000034, IPR001791, IPR002049, IPR008211, IPR013032, IPR013320 |
| Unigene ID: |
Dm.3218 |
| RefSeq IDs: |
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| Homologene Group: |
Homologene:122804: Anopheles gambiae str. PEST - AgaP_AGAP007849 |
| Other External Accs: |
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| Kc167: |
0.481702 - Not expressed |
BG3: |
8.53366 - Expressed |
| Clone 8: |
0.341668 - Not expressed |
S2-DRSC: |
5.26206 - Expressed |
| S2R+: |
2.35943 - Weakly expressed |
| Kc167: |
0.0491081 - Not expressed |
BG3: |
0.190569 - Not expressed |
| Clone 8: |
0 - Not expressed |
S2-DRSC: |
0.208132 - Not expressed |
| S2R+: |
0 - Not expressed |
