Reagents and Results:
|
|
|
|
Cell Screening dsRNAs (3)
|
|
|
|
|
|
Hits in 2 of 83 screens (Click title to view all hits in that screen)
|
|
|
|
Nuclear targeting factor of Mad (pmid: 17785517 )
|
|
CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca2+ Entry (pmid: 16645049 )
|
|
General dsRNA/Amplicon Information
|
|
|
| Amplicon ID: |
DRSC07980 |
Sequence: |
CAGATAGGGCAATATATCAATTATCTTCTTCGATAGACCCTGAGGTGCGTTGCGATCTACGAAGTCAACAAACTCTAGCTCGTAGTTTAAAGCTGGAGCCGTTTGTTCCCTAAACTTTTGCAACTTGACATCTGGGGTTGGAATATCACCAGAGGTTAGGGGTTCAGTGGTATCAGGTGCAGTTCGATCAGAGTCCGGCTTCCCAGTTTCCTGGCTCGAAGCTTCTGGTATTTGGTTTGCAGTGGTTTGGGTTTCTTGTGCATTCTCTACATTCTTAGGACTGTATACCACCGCTGCAATGGGGGAAGTAAAGTTATGAAACAGCTCTGAGATCTTCTGGAGCAGCGAATCC |
| "R" Primer: |
GGATTCGCTGCTCCAGAA |
| "S" Primer: |
CAGATAGGGCAATATATCAATTAT |
| Amplicon Length: |
352 |
| Target Gene(s): |
CG32298, CG32299 |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
|
dsRNA Matches to Transcripts and Exons
|
|
|
| Transcript |
FBtr0299807 |
| Exon 4 length |
507 |
dsRNA Position Relative to Exon 4: 57 - 408 |
| Transcript |
FBtr0299808 |
| Exon 6 length |
507 |
dsRNA Position Relative to Exon 6: 57 - 408 |
|
Historical Amplicon Information
|
|
|
| HFA Amplicon ID: |
HFA07980 |
| Amplicon Nickname: (Old Oligo Name) |
AE002584_sanger_845 |
|
Click
here for amplicon info documentation
|
|
Genomic Position Information (based on Flybase, release 5.28)
|
|
|
| Chromosome Length: |
|
 |
| Start: |
2388582 |
| End: |
2392933 |
|
|
|
General dsRNA/Amplicon Information
|
|
|
| Amplicon ID: |
DRSC22354 |
Sequence: |
AAAAGTTCAACTTTCCGATGGTCTGTTTCCGAAATTTGTAAGACGCTGGTTCTTGGACATCCAATTGCATGATTTTTGAAGGCTTTCAAGAAGACTTCGCCACTCTATTTGCATTCATCAAACGCAAGCGACTGGCGTTTCTGAAAACGGTATATGTGCCGGCCATGATAATGAAAAGTTGCACCACTTTGGCTTGATAGTAGGTCTAAGATGATAATGTGTTTAGATAAAAGCTTATAGCAAACCGAAGCAACAAACGACTCACCTGTCTTATATAGATGGGCAATGGAATGTGCGATCGATGAACATGGTAATCCATTCCTCTGAACAGCCAATAGCCCGGCCACGCCCCCAAACTGGCGAAAAGAAATGGTGCTGCAGTACTGTAGGCCTTTTCAAAGGATTGCGAATGCACCAGTAAACGTATCTCCTCGCCAAGATTATGCATCCGAGGTGGGTGGGCTATTGCCTTGGCTGGTTCCTCCTCCTTTCGCTTAAGCCTCGTCGAGAACTTCTCCTTCAGATTGCCGTAATAATTTTGGATGCCCATCTTGGAAAGGTTTAATTTTCG |
| "R" Primer: |
CGAAAATTAAACCTTTCCAAGA |
| "S" Primer: |
AAAAGTTCAACTTTCCGATGG |
| Amplicon Length: |
571 |
| Target Gene(s): |
CG32299, CG32298 |
| Potential Off-Target Number: |
1 |
| CAR/CAN repeats: |
None |
|
dsRNA Matches to Transcripts and Exons
|
|
|
| Transcript |
FBtr0072898 |
| Exon 2 length |
241 |
dsRNA Position Relative to Exon 2: -365 - 205 |
| Exon 4 length |
334 |
dsRNA Position Relative to Exon 4: 29 - 599 |
| Transcript |
FBtr0299808 |
| Exon 1 length |
259 |
dsRNA Position Relative to Exon 1: -365 - 205 |
| Exon 3 length |
285 |
dsRNA Position Relative to Exon 3: -20 - 550 |
| Transcript |
FBtr0299807 |
| Exon 1 length |
259 |
dsRNA Position Relative to Exon 1: -365 - 205 |
| Exon 2 length |
285 |
dsRNA Position Relative to Exon 2: -20 - 550 |
|
Genomic Position Information (based on Flybase, release 5.28)
|
|
|
| Chromosome Length: |
|
 |
| Start: |
2387636 |
| End: |
2392206 |
|
|
|
General dsRNA/Amplicon Information
|
|
|
| Amplicon ID: |
DRSC25827 |
Sequence: |
AAAAGTTGCACCACTTTGGCTTGATAGTAGGTCTAAGATGATAATGTGTTTAGATAAAAGCTTATAGCAAACCGAAGCAACAAACGACTCACCTGTCTTATATAGATGGGCAATGGAATGTGCGATCGATGAACATGGTAATCCATTCCTCTGAACAGCCAATAGCCCGGCCACGCCCCCAAACTGGCGAAAAGAAATGGTGCTGCAGTACTGTAGGCCTTTTCAAAGGATTGCGAATGCACCAGTAAACGTATCTCCTCGCCAAGATTATGCATCCGAGGTGGGTGGGCTATTGCCTTGGCTGGTTCCTCCTCCTTTCGCTTAAGC |
| "R" Primer: |
GCTTAAGCGAAAGGAGGAGG |
| "S" Primer: |
AAAAGTTGCACCACTTTGGC |
| Amplicon Length: |
327 |
| Target Gene(s): |
CG32298, CG32299 |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
|
dsRNA Matches to Transcripts and Exons
|
|
|
| Transcript |
FBtr0299807 |
| Exon 1 length |
259 |
dsRNA Position Relative to Exon 1: -294 - 32 |
| Exon 2 length |
285 |
dsRNA Position Relative to Exon 2: 51 - 377 |
| Transcript |
FBtr0072898 |
| Exon 4 length |
334 |
dsRNA Position Relative to Exon 4: 100 - 426 |
| Exon 2 length |
241 |
dsRNA Position Relative to Exon 2: -294 - 32 |
| Transcript |
FBtr0299808 |
| Exon 3 length |
285 |
dsRNA Position Relative to Exon 3: 51 - 377 |
| Exon 1 length |
259 |
dsRNA Position Relative to Exon 1: -294 - 32 |
|
Genomic Position Information (based on Flybase, release 5.28)
|
|
|
| Chromosome Length: |
|
 |
| Start: |
2387809 |
| End: |
2392135 |
|
|
|
Transgenic RNAi Project (TRiP) Fly Stocks (0)
|
|
|
Gene Information for
CG32298:
|
|
|
|
| Symbol: |
CG32298 |
Gene Name: |
CG32298 |
| Flybase ID: |
FBgn0052298 |
Entrez Gene: |
317963 |
| Synonyms: |
NEST:bs10a03, CG32298 |
| Old Flybase IDs: |
FBgn0045346 |
| NCBI Nucleotide Accs: |
AI945188 |
| NCBI Protein Accs: |
AAN12221 |
| UniProt Accs: |
Q8IRF1 |
| Gene Ontology IDs: |
GO:0003674, GO:0005575, GO:0008150 |
| Protein Domains: |
|
| Unigene ID: |
Dm.6266 |
| RefSeq IDs: |
|
| Homologene Group: |
|
| Other External Accs: |
|
| Kc167: |
0.168283 - Not expressed |
BG3: |
0.0544199 - Not expressed |
| Clone 8: |
0.0643141 - Not expressed |
S2-DRSC: |
0.356612 - Not expressed |
| S2R+: |
0 - Not expressed |
