Reagents and Results:
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Cell Screening dsRNAs (2)
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Hits in 9 of 88 screens (Click title to view all hits in that screen)
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DCV Screen (pmid: 17040126 )
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S2R+ Morphology Screen (pmid: 21893601 )
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Hh Signalling (pmid: 16311596 )
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MAPK Signaling Pathway Screen (pmid: 17086199 )
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Genes involved in protein secretion and Golgi organization (pmid: 16452979 )
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Genes regulating lipid storage in Drosophila melanogaster
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Comparison of GPCR Induced vs. Small G-Protein Induced Signalling to the Nucleus (pmid: 23177739 )
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RTK Specificity (pmid: 22028469 )
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ERK Signalling Dynamics (pmid: 22028469 )
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC03495 |
Sequence: |
GGGGGATTCCGGTACAAGATTGCCAAGTCGCTCATCATGTCCAAGGTGAAGCAGGCCCTGGGCTTCGATCGCGTCCTTACACTGGCCAGTGCGGCAGCTCCCATGTCGCCGGAGACGAAGAAGTACTTCCTCAGTCTGGACCTAAAGATTGTCGATGCCTTCGGCATGTCAGAAACGGCCGGTTGTCACACCATCTGCCTTCCCGATTCCGTGGGTCTGAACACAATCGGCAAAACTTTGCCCGGCTGCGAGTCCAAGTTCATCAACAAGGATGCCAACGGTCACGGAGAGCTGTGCATCCGAGGACGTCACGTTTTCATGGGCTACATCGACAACAAGGAGAAGACCGAGGAGTCGCTGGATGACGACTGCTGGCTGCATTCCGGTGATTTGGGATTTGTGGATGACAAGGGTTATGTTTCACTGACGGGACGATCCAAGGAGATCATCATTACCGCCGGCGGCGAGAACATACCGCCAGTGCACATCGAGAACACGATCAAGAAGGAG |
| "R" Primer: |
GGGGGATTCCGGTACAAG |
| "S" Primer: |
CTCCTTCTTGATCGTGTTCT |
| Amplicon Length: |
510 |
| Target Gene(s): |
bgm |
| Potential Off-Target Number: |
1 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0080590 |
| Exon 5 length |
592 |
dsRNA Position Relative to Exon 5: 11 - 520 |
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Historical Amplicon Information
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| HFA Amplicon ID: |
HFA03495 |
| Amplicon Nickname: (Old Oligo Name) |
bgm |
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Click
here for amplicon info documentation
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14016045 |
| End: |
14020554 |
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Hits in 1 of 42 screens (Click title to view all hits in that screen)
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A genome-wide Drosophila RNAi screen for the mitochondrial calcium uniporter (pmid: 19797662 )
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC27103 |
Sequence: |
CGACGTGGAGGATCAGTACATGACCCGTTTGGAGAATGTGGCGATCAACGAGTGCTGCTGCCTGGTCTACACCTCCGGAACGGTGGGCATGCCCAAGGGCGTGATGCTCTCCCACGACAACATCACCTTCGATGTGCGCGGCATCGTCAAGGCCATGGACCGTGTGGTGGTTGGGGCGGAGTCGATCGTCTCCTACCTGCCACTTTCGCACGTGGCCGCCCAGACCGTGGACATTTACACCTGCGCCTTTGTGGCGGGCTGCATTTGGTTCGCCGACAAGGATGCGCTGAAGGGAACGCTGGTGAAGTCGTTGCAGGATGCGCGACCCACGCGATTCATGGGCGTGCCGCGTGTGTACGAGAAGTTCCAGGAGCGAATG |
| "R" Primer: |
CGACGTGGAGGATCAGTACA |
| "S" Primer: |
CATTCGCTCCTGGAACTTCT |
| Amplicon Length: |
379 |
| Target Gene(s): |
bgm |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0080590 |
| Exon 4 length |
500 |
dsRNA Position Relative to Exon 4: 30 - 408 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14014567 |
| End: |
14018945 |
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Cell Screen Follow-Up dsRNAs (2)
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC32269 |
Sequence: |
CCAAGGCGTTCATTAAGCTCGGTCTGGAGGAGCACCATTCGGTGGGTGTGCTGGCCTTCAATTGCGCCGAATGGTTCTACTCGGCCATGGGCGCCATTCACGCACGAGGCATCATCGCCGGAATCTACACCACCAATTCCGCCGATGCAGTGCAGCACGTTCTGGAGAGCTCACATGCCCAAATCGTGGTCGTCGACGACGCCAAGCAAATGGACAAGATTCACGCC |
| "R" Primer: |
CCAAGGCGTTCATTAAGCTC |
| "S" Primer: |
GGCGTGAATCTTGTCCATTT |
| Amplicon Length: |
227 |
| Target Gene(s): |
bgm |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0080590 |
| Exon 3 length |
346 |
dsRNA Position Relative to Exon 3: 30 - 256 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14014161 |
| End: |
14018387 |
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC32270 |
Sequence: |
AGACGCGCTCTACAATCGTCCTGGGCCCAACCGCCTGCGGCAGGCGGATGCCTATCGCACCACCAATCGTCAGGATGCCGTCAAGATTCGTATGGCCAAGGATGGAATCGGCGCAGAGGAGCCCATCTCCGTGCCCGGCCTGCTGAAGCGTACGGTCAACAATTATGGCGACTATCCTGCGCTGCGCACCAAGAACGGCAAGAACGGATATCAC |
| "R" Primer: |
AGACGCGCTCTACAATCGTC |
| "S" Primer: |
GTGATATCCGTTCTTGCCGT |
| Amplicon Length: |
214 |
| Target Gene(s): |
bgm |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0080590 |
| Exon 2 length |
248 |
dsRNA Position Relative to Exon 2: 21 - 234 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
14013807 |
| End: |
14018020 |
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Transgenic RNAi Project (TRiP) Fly Stocks (3)
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Long Hairpin JF03054
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General Hairpin Information
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| TRiP #: |
JF03054 |
BDSC Stock #: |
28639 |
| Gene ID: |
CG4501 |
Gene Name: |
bgm |
| Vector: |
VALIUM10 |
Hairpin ID: |
TR02546P.1 |
| Classifications: |
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Sense Oligo: |
AAGAATTCGTCAGGATGCCGTCAAGATT |
| Antisense Oligo: |
AGTCTAGAATCTTGTCCATTTGCTTGGC |
| Insertion Location: |
attP2 |
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Short Hairpin HMC04419
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General Hairpin Information
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| TRiP #: |
HMC04419 |
BDSC Stock #: |
56979 |
| Gene ID: |
CG4501 |
Gene Name: |
bgm |
| Vector: |
VALIUM20 |
Hairpin ID: |
SH06638.N |
| Classifications: |
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Sense Oligo: |
ctagcagtCACGATAGACGCGCTCTACAAtagttatattcaagcataTTGTAGAGCGCGTCTATCGTGgcg |
| Antisense Oligo: |
aattcgcCACGATAGACGCGCTCTACAAtatgcttgaatataactaTTGTAGAGCGCGTCTATCGTGactg |
| Insertion Location: |
attP40 |
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Short Hairpin HMC04204
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General Hairpin Information
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| TRiP #: |
HMC04204 |
BDSC Stock #: |
55918 |
| Gene ID: |
CG4501 |
Gene Name: |
bgm |
| Vector: |
VALIUM20 |
Hairpin ID: |
SH06617.N |
| Classifications: |
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Sense Oligo: |
ctagcagtCTGCGAGTCCAAGTTCATCAAtagttatattcaagcataTTGATGAACTTGGACTCGCAGgcg |
| Antisense Oligo: |
aattcgcCTGCGAGTCCAAGTTCATCAAtatgcttgaatataactaTTGATGAACTTGGACTCGCAGactg |
| Insertion Location: |
attP2 |
Gene Information for
bgm:
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| Symbol: |
bgm |
Gene Name: |
bubblegum |
| Flybase ID: |
FBgn0027348 |
Entrez Gene: |
44117 |
| Synonyms: |
Bgm, BG:DS01514.2, unnamed, BG:DS0514.2, Bubblegum, 2a9hom, CG4501 |
| Old Flybase IDs: |
FBgn0027881, FBgn0028908 |
| NCBI Nucleotide Accs: |
AA202657, AA391612, AA696773, AA698803, AA803692, AE003408, AI062220, AI062891, AI064646, AI404572, AI518984, AI945524, AQ025909, AY118315, BH023729, CL610130, CZ471647, CZ471684, CZ476540, CZ482905 |
| NCBI Protein Accs: |
AAM48344, AAF53368, AHN54437 |
| UniProt Accs: |
B5RIV0 |
| Gene Ontology IDs: |
GO:0004467, GO:0001676, GO:0005575, GO:0034976 |
| Protein Domains: |
IPR000873 |
| Unigene ID: |
Dm.23376 |
| RefSeq IDs: |
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| Homologene Group: |
Homologene:57133: Xenopus tropicalis - acsbg2, Anopheles gambiae str. PEST - AgaP_AGAP008596, Danio rerio - acsbg2, Gallus gallus - ACSBG2, Rattus norvegicus - Acsbg2, Mus musculus - Acsbg2, Bos taurus - ACSBG2, Canis lupus familiaris - LOC476732, Macaca mulatta - ACSBG2, Pan troglodytes - ACSBG2, Homo sapiens - ACSBG2 |
| Other External Accs: |
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| Kc167: |
2.5959 - Weakly expressed |
BG3: |
0.355328 - Not expressed |
| Clone 8: |
0.0239065 - Not expressed |
S2-DRSC: |
8.26635 - Expressed |
| S2R+: |
0.894021 - Not expressed |
