Reagents and Results:
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Cell Screening dsRNAs (1)
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Hits in 2 of 88 screens (Click title to view all hits in that screen)
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A genome-wide RNAi screen reveals multiple regulators of caspase activation (pmid: 17998402 )
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Nuclear targeting factor of Mad (pmid: 17785517 )
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC11231 |
Sequence: |
GAATGAGCCGCTGGGAGCACTCACCCCTTATGTGGGTGTCGTTGACGGTCCCGAGGTAAAAAAATCGAAAAAGATACATGGTGGGGATAGTGCCATATTGGGCACATATAAGATGCAATCACGTTTCAACCGAGGCGTTTTGCTAATGGTTAACATAATGGACTATCCGGATCAAAACCGTCGACGGATCGGAGCCGAAAAGGACAGCAAGTCGTTGATACACTTGTTTCAAGAACTGAATTTTACGATTTTCCCCTATGGGAACGTGAATCAGGATCAGTTCTTTAAACTTCTGACAATGGTGACCTCCTCGTCGTATGTGCAGAATACCGAGTGTTTCGTAATGGTACTGATGACACACGGCAACAGTGTGGAGGGAAAAGAGAAGGTGGAGTTTTGCGATGGATCTGTGGTCGATATGCAGAAGATCAAGGACCATTTCCAGACGGCCAAATGTCCTTATTTGGTGAACAAGCCGAAGGTGCTTATGTTTCCCTTTTGCCG |
| "R" Primer: |
GAATGAGCCGCTGGGAG |
| "S" Primer: |
CGGCAAAAGGGAAACATAAG |
| Amplicon Length: |
504 |
| Target Gene(s): |
Dronc |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0076347 |
| Exon 2 length |
1159 |
dsRNA Position Relative to Exon 2: 6 - 509 |
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Historical Amplicon Information
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| HFA Amplicon ID: |
HFA11231 |
| Amplicon Nickname: (Old Oligo Name) |
Nc |
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Click
here for amplicon info documentation
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
9960949 |
| End: |
9965452 |
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Cell Screen Follow-Up dsRNAs (1)
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General dsRNA/Amplicon Information
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| Amplicon ID: |
DRSC39212 |
Sequence: |
CACTGCCAATTGGAGACTGATCACGGCAGCCATCCTTGGCGCGCCCAATAAGCGGAGTCACCGGAACGCGTGGAAGCCATATCCGGAATGCAGCCGCCGGAGCTCGAGATTGGAATGCCGAAGAGGCATCGCGAGCATATACGCAAGAATCTGAATATACTGGTTGAATGGACGAACTACGAGCGTCTGGCCATGGAGTGCGTGCAACAGGGCATCCTAACCGTTCAGATGCTAAGAAATACGCAGGATCTCAATGGCAAGCCATTCAACATGGACGAGAAGGATGTGCGTGTGGAGCAGCATCGTAGGCTCCTATTGAAGATCACCCAGCGTGGTCCCACCGCCTATAACCTGCTGATCAATGCACTGCGCAATATCAATTGTCTGGATGCGGCCGTTCTATTGGAATCCGTCGATGAGTCCGATTCAAGGCCACCCTTTATCTCGCTAAACGAACGGAGAACCAGC |
| "R" Primer: |
CACTGCCAATTGGAGACTGA |
| "S" Primer: |
GCTGGTTCTCCGTTCGTTTA |
| Amplicon Length: |
468 |
| Target Gene(s): |
Dronc |
| Potential Off-Target Number: |
0 |
| CAR/CAN repeats: |
None |
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dsRNA Matches to Transcripts and Exons
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| Transcript |
FBtr0076347 |
| Exon 1 length |
892 |
dsRNA Position Relative to Exon 1: 359 - 826 |
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Genomic Position Information (based on Flybase, release 5.28)
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| Chromosome Length: |
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| Start: |
9959901 |
| End: |
9964368 |
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Transgenic RNAi Project (TRiP) Fly Stocks (1)
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Short Hairpin HMS00758
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General Hairpin Information
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| TRiP #: |
HMS00758 |
BDSC Stock #: |
32963 |
| Gene ID: |
CG8091 |
Gene Name: |
Dronc |
| Vector: |
VALIUM20 |
Hairpin ID: |
SH00696.N |
| Classifications: |
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Sense Oligo: |
ctagcagtAAGAATCGACAAGTAGCGATAtagttatattcaagcataTATCGCTACTTGTCGATTCTTgcg |
| Antisense Oligo: |
aattcgcAAGAATCGACAAGTAGCGATAtatgcttgaatataactaTATCGCTACTTGTCGATTCTTactg |
| Insertion Location: |
attP2 |
Gene Information for
Dronc:
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| Symbol: |
Dronc |
Gene Name: |
Death regulator Nedd2-like caspase |
| Flybase ID: |
FBgn0026404 |
Entrez Gene: |
39173 |
| Synonyms: |
anon-WO0118547.313, Nedd2-like caspase, dronc, Nedd 2-like caspase, caspase-9, Drosophila Caspase-9 homologue, Caspase-9-like, CG8091, anon-EST:GreesD6, Nc, Nc\Dronc, CG8090, anon-EST:GressD6, Drosophila Nedd2-like caspase, Dronc/Casp9, Death regulator Nedd2-like caspase |
| Old Flybase IDs: |
FBgn0025326, FBgn0062102 |
| NCBI Nucleotide Accs: |
AA438366, AA540020, AA949406, AA950690, AF104357, AI296149, AJ242796, AW944122, AX094189, AY058747, CL526027, CL868556, X65271 |
| NCBI Protein Accs: |
AAF50180, AAD26625, CAB53565, AAL13976 |
| UniProt Accs: |
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| Gene Ontology IDs: |
GO:0097199, GO:2001269, GO:0008258, GO:0006915, GO:0007516, GO:0048749, GO:0007417, GO:0048102, GO:0035071, GO:0005739, GO:0005737, GO:0001700, GO:0046668, GO:0007291, GO:0007423, GO:0007552, GO:0008340, GO:0004175, GO:0042803, GO:0016540, GO:0031638, GO:0006919, GO:0004197, GO:0046672, GO:0035070, GO:0010623, GO:0012501, GO:0016322, GO:0048813, GO:0010941, GO:0043293, GO:0050700, GO:0051291, GO:0045476, GO:0008285, GO:0035234, GO:0010506, GO:0035006 |
| Protein Domains: |
IPR001309, IPR002138, IPR011029, IPR011600, IPR015917, IPR016129, IPR017350, IPR029030 |
| Unigene ID: |
Dm.3230 |
| RefSeq IDs: |
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| Homologene Group: |
Homologene:76771: Anopheles gambiae str. PEST - CASPL2 |
| Other External Accs: |
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| Kc167: |
14.844 - Expressed |
BG3: |
38.2798 - Expressed |
| Clone 8: |
33.0316 - Expressed |
S2-DRSC: |
19.1385 - Expressed |
| S2R+: |
15.5721 - Expressed |
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