DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment PUM1 and pum

DIOPT Version :9

Sequence 1:	NP_001018494.1	Gene:	PUM1 / 9698	HGNCID:	14957	Length:	1188	Species:	Homo sapiens
Sequence 2:	NP_001262403.1	Gene:	pum / 41094	FlyBaseID:	FBgn0003165	Length:	1533	Species:	Drosophila melanogaster

Alignment Length:	1392	Identity:	519/1392 - (37%)
Similarity:	647/1392 - (46%)	Gaps:	397/1392 - (28%)

- Green bases have known domain annotations that are detailed below.

Human    20 PHLKH-----HPQEPANPNMPVVLTSG--------------TGSQAQPQPAANQALAAGTHSSPV 65
            ||.:|     ..|:..|...|..|..|              .|.|.|.|....|....|.:::.:
  Fly   219 PHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHPGQYNANL 283

Human    66 PGSIGVAGRSQDDA-----MVDYFFQRQHGEQLGG-------GGSGGGG-----YNNSKHRWPTG 113
            .......|.....|     |.|:.....|....||       |..|.||     |...:...|.|
  Fly   284 LNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGGQTTVPMG 348

Human   114 DNIHAEHQVRSMDELNH--DFQALALEGRAMGEQLLPGKKFWETDE------SSKDGP------K 164
                    ...|...|.  :..|:|...|.........||.||..:      |:..||      .
  Fly   349 --------AAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIP 405

Human   165 GIFLGDQ---WRDSAWGTSDHS--VSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEY 224
            ||  ||.   |:|..|.|...:  |..|.......|.|...||....:||||..:   ...:|||
  Fly   406 GI--GDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDST---CAKVVEY 465

Human   225 VLSSSPGDSCLRKGGFGP--RDADSDENDKG-EKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQN 286
            |.|.||.:......|..|  |:...|:|||. :.|.|.....|..| ||::..|   ||...|.|
  Fly   466 VFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNG-LKKDDQV---TNSNGVVN 526

Human   287 GIDADVKDFSRTPGNCQNSANE------VDLLGP------------------------NQNGSEG 321
            |||.| |.|:||||:.|.|..|      .:||.|                        :.||..|
  Fly   527 GIDDD-KGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGG 590

Human   322 --------------------LAQL-----TSTNGAKPVEDFSNM-ESQSVPLDPMEHVG--MEPL 358
                                ::||     ....|..|:...|.| ..|:...:.||..|  ::..
  Fly   591 GSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQ 655

Human   359 QFDY------------------------SGTQVPVDSAAATVGL---------------FDYNSQ 384
            .||.                        :.......||||.||.               .....|
  Fly   656 NFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQ 720

Human   385 QQLFQRPNALAVQQLTAAQQQ-QYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTD--PYTAGLA 446
            ||..|...|.|.||..||||| |.|..||.|           ...|:|:   ||.:  || .|:.
  Fly   721 QQQQQMHMAAASQQFLAAQQQAQNAAYAAQQ-----------ATSYVIN---PGQEAAPY-MGMI 770

Human   447 AAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGASQR 511
            |||.:       .||||.|||:||.:|..||                            |...:|
  Fly   771 AAAQM-------PYYGVAPWGMYPGNLIPQQ----------------------------GTQPRR 800

Human   512 PLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLG 576
            ||||  :|||.:..|                       |.|:  .|:.|.||:|:: .|.|.  |
  Fly   801 PLTP--SQQGAENQP-----------------------YQVI--PAFLDHTGSLLM-GGPRT--G 835

Human   577 APVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQ--QPQPQPQQQPNNN 639
            .|:|||:||||::...|.:|                     ||..|.|.|  |||||..||...:
  Fly   836 TPMRLVSPAPVLVPPGATRA---------------------GPPPPQGPQLYQPQPQTAQQNLYS 879

Human   640 LASSSFYGNNSLNSNS---------QSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTA 695
            ..:.|..|..:||::|         :|:|.||..:....::.:. .:::::.:|:..|......|
  Fly   880 QQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVA-AAANAVNSTVAQAAAAAAAA 943

Human   696 VANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGL-SFSSSPGPVGMPLPSQGPGHSQTP 759
            .|......|:...:.:|:                  |..| :.::|..|:|.|:         ||
  Fly   944 AAARGKWPGAMSGAASGA------------------YGALGAGNASASPLGAPI---------TP 981

Human   760 PPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRS----------------------ASSAS 802
            ||      |:.|..||          |.|||||::.|.                      |::|:
  Fly   982 PP------SAQSCLLG----------SRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAAN 1030

Human   803 SLFSPSSTLFSS-----------------------------------SRLRYGMSDVMPSGRSRL 832
            ::|..:|::||:                                   :....|:......|||||
  Fly  1031 NMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRL 1095

Human   833 LEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVF 897
            ||||||.||||||||::|.||:|||||||||||||.||||||.||:|:||:|||.|||.||.|||
  Fly  1096 LEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVF 1160

Human   898 GNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGH 962
            ||||||||||||:.|||..|..:::||||.||||||||||||||||.|..:||  .|:|.|||||
  Fly  1161 GNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQ--QEIVHELDGH 1223

Human   963 VLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPI 1027
            |||||||||||||||||||||.|.:|||||:||||||::||||||||||||||||||..:||.||
  Fly  1224 VLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPI 1288

Human  1028 LEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHAS 1092
            |:|||:|||||:||||||||||||||||:.||||.::..:||.|||||||||||||||||||||:
  Fly  1289 LDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHAT 1353

Human  1093 RTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYT 1157
            |.||..|||||||.||   :||:.|||||||||||||||||:||.|.|.:|.|||||:|.|||||
  Fly  1354 RGERTGLIDEVCTFND---NALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYT 1415

Human  1158 YGKHILAKLEKYYMK--NGVDLGPICG 1182
            |||||.|||||||||  |.:.:|...|
  Fly  1416 YGKHINAKLEKYYMKITNPITVGTGAG 1442

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
PUM1	NP_001018494.1	Pumilio 6. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	1030..1065		28/34 (82%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718, ECO:0000269\|PubMed:21397187, ECO:0007744\|PDB:3BSB	1045..1049		3/3 (100%)
		Pumilio 7. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	1066..1101		27/34 (79%)
		Guanine-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718, ECO:0000269\|PubMed:21397187, ECO:0007744\|PDB:3BSB	1081..1085		3/3 (100%)
		Pumilio 8. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	1105..1144		26/38 (68%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718, ECO:0000269\|PubMed:21397187, ECO:0007744\|PDB:1M8W, ECO:0007744\|PDB:1M8X, ECO:0007744\|PDB:3BSB, ECO:0007744\|PDB:3BSX, ECO:0007744\|PDB:3Q0L, ECO:0007744\|PDB:3Q0M, ECO:0007744\|PDB:3Q0N, ECO:0007744\|PDB:3Q0O, ECO:0007744\|PDB:3Q0P	1124..1128		3/3 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	22..73		12/69 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	233..272		13/41 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	485..524		9/38 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	613..648		13/36 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	742..773		9/30 (30%)
		Pumilio	843..1166	CDD:153420	249/322 (77%)
		Pumilio 1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	848..883		26/34 (76%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718	863..867		3/3 (100%)
		Pumilio 2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	884..919		27/34 (79%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718	899..903		3/3 (100%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718	935..939		3/3 (100%)
		Pumilio 4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	958..993		31/34 (91%)
		Non-specific-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718	973..977		3/3 (100%)
		Pumilio 5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	994..1029		28/34 (82%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000269\|PubMed:18328718	1009..1013		3/3 (100%)
pum	NP_001262403.1	Pumilio	1106..1424	CDD:153420	249/322 (77%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165148149
Domainoid	1	1.000	146	1.000	Domainoid score	I4546
eggNOG	1	0.900	-	-		E1_COG5099
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	640	1.000	Inparanoid score	I817
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000719
OrthoInspector	1	1.000	-	-		otm40372
orthoMCL	1	0.900	-	-		OOG6_100436
Panther	1	1.100	-	-	O	PTHR12537
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X849
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	11	10.840