Protein Alignment RAPGEF2 and CDC25

DIOPT Version :10

• Sequence 1: NP_001338653.1
• Gene: RAPGEF2 / 9693
• HGNCID: 16854
• Length: 1685
• Species: Homo sapiens
• Sequence 2: NP_013413.1
• Gene: CDC25 / 851019
• SGDID: S000004301
• Length: 1589
• Species: Saccharomyces cerevisiae
• Alignment Length: 909
• Identity: 184/909 - (20%)
• Similarity: 334/909 - (36%)
• Gaps: 233/909 - (25%)

Human   369 EYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRE-LD-------RTG 425
            |::|.:.:.|              .||.| :|.|....|:..:.|::.|..| ||       :..
Yeast   838 EHLKIISKPK--------------SRIRN-LEINSSTYEQINQNVLLLEILENLDLSIFINLKNL 887

Human   426 TRKGHIVIKGTSERLTMHLVEEHSVVDPTFIE------DFLL-----TYRTFLSSP--------- 470
            .:...|::...||...:|.:...|.|...|.:      |.::     |.:|.|..|
Yeast   888 IKTPSILLDLESEEFLVHAMSSVSSVLTEFFDIKQAFHDIVIRLIMTTQQTTLDDPYLFSSMRSN 952

Human   471 MEVGKKLLEWF----NDPSLRDKVTRV----------VLLWVNNHFNDFEGDPAMTRFL---EEF 518
            ..||..  |.|    |.|.::....:.          ||:..:..||:.|       ||   ::|
Yeast   953 FPVGHH--EPFKNISNTPLVKGPFHKKNEQLALSLFHVLVSQDVEFNNLE-------FLNNSDDF 1008

Human   519 ENNLEREKMGGHLRLLNIAC----------------AAKAKRRLMTLTKPSREAPLPFILLGGSE 567
            ::..|:     ::.:.|:||                ||:..:..:|..          :|.|..|
Yeast  1009 KDACEK-----YVEISNLACIIVDQLIEERENLLNYAARMMKNNLTAE----------LLKGEQE 1058

Human   568 KGFGIF-VDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNL--F 629
            |.|.|: .|..|..|:..||.:..     |:..:::  |:...|            .::.||  |
Yeast  1059 KWFDIYSEDYSDDDSENDEAIIDD-----ELGSEDY--IERKAA------------NIEKNLPWF 1104

Human   630 VFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKL 694
            :..:..|.|..:.|.......|...|:..:.:.:.|.|.:|..:|....:        :..|...
Yeast  1105 LTSDYETSLVYDSRGKIRGGTKEALIEHLTSHELVDAAFNVTMLITFRSI--------LTTREFF 1161

Human   695 KKILDKTRISILPQK--PYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRI 757
            ..::  .|.::.|.:  .|:|..|...:..:.:..|....:.|.|....|.:...|.:    ..|
Yeast  1162 YALI--YRYNLYPPEGLSYDDYNIWIEKKSNPIKCRVVNIMRTFLTQYWTRNYYEPGI----PLI 1220

Human   758 LDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEG 822
            |:|:.                    :::|:.....|.::|.|.|..:.....:      .|.|
Yeast  1221 LNFAK--------------------MVVSEKIPGAEDLLQKINEKLINENEKE------PVDP-- 1257

Human   823 VIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLS 887
              ||:   |.:|.:....:...:..:  :|.:.:|.|.. :....|..::.||.:.....||||:
Yeast  1258 --KQQ---DSVSAVVQTTKRDNKSPI--HMSSSSLPSSA-SSAFFRLKKLKLLDIDPYTYATQLT 1314

Human   888 MRNFELFRNIEPTEYIDDLF--KLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIK 950
            :...:|:..|...|.:|..:  |..:.....|:.:|....|..|.:|:..|:::.:...|.|:.:
Yeast  1315 VLEHDLYLRITMFECLDRAWGTKYCNMGGSPNITKFIANANTLTNFVSHTIVKQADVKTRSKLTQ 1379

Human   951 HFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYR 1015
            :|:.:|.||:|..||:||.||:|.|..:|:.||:.||:.:..:.:.|.::|.:|.|..||..|||
Yeast  1380 YFVTVAQHCKELNNFSSMTAIVSALYSSPIYRLKKTWDLVSTESKDLLKNLNNLMDSKRNFVKYR 1444

Human  1016 NVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDS---KVDGLVNFEKLRMIAKEIRHVGRMASVNMD 1077
            .:|.|.. ....:|.|.|...||||...||..   ....::||.|...||..:..:
Yeast  1445 ELLRSVT-DVACVPFFGVYLSDLTFTFVGNPDFLHNSTNIINFSKRTKIANIVEEI--------- 1499

Human  1078 PALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYL 1142
              :.|:....|.:.|..:.      ||::             :|..|...:         .|||
Yeast  1500 --ISFKRFHYKLKRLDDIQ------TVIE-------------ASLENVPHI---------EKQY- 1533

Human  1143 SNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPP 1206
                         .||||.||.:.....:........::|:.........:....|...:::||
Yeast  1534 -------------QLSLQVEPRSGNTKGSTHASSASGTKTAKFLSEFTDDKNGNFLKLGKKKPP 1584

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAPGEF2	NP_001338653.1	CAP_ED	37..143	CDD:237999
		cNMP	297..412	CDD:197516	9/42 (21%)
		RasGEFN	428..541	CDD:214571	31/165 (19%)
		PDZ_RapGEF2_RapGEF6-like	545..627	CDD:467237	15/82 (18%)
		RA_PDZ-GEF1	769..851	CDD:340483	11/81 (14%)
		RasGEF	874..1099	CDD:238087	65/229 (28%)
CDC25	NP_013413.1	SH3_Sdc25	62..122	CDD:212816
		RasGEFN	1117..1246	CDD:214571	26/162 (16%)
		RasGEF	1301..1543	CDD:214539	78/295 (26%)