Protein Alignment RAPGEF2 and Sos

DIOPT Version :10

• Sequence 1: NP_001338653.1
• Gene: RAPGEF2 / 9693
• HGNCID: 16854
• Length: 1685
• Species: Homo sapiens
• Sequence 2: NP_476597.2
• Gene: Sos / 34790
• FlyBaseID: FBgn0001965
• Length: 1596
• Species: Drosophila melanogaster
• Alignment Length: 1742
• Identity: 307/1742 - (17%)
• Similarity: 499/1742 - (28%)
• Gaps: 775/1742 - (44%)

Human   218 SSLSDIYQATESEAGDMD-------------LSGLPETAVDSEDDDDEEDIERASDPLMSRDIVR 269
            |::.|||:.|.:..|.::             :....|...::|:.|..:..........|||.:.
  Fly   293 SNIMDIYEVTVTLLGSLEDVIEMSQEQSAPCVGSCFEELAEAEEFDVYKKYAYDVTSQASRDALN 357

Human   270 DCLEKD--------------------------PI----------------DRTDDDI---EQLLE 289
            :.|.|.                          ||                ..:.|||   ||:..
  Fly   358 NLLSKPGASSLTTAGHGFRDAVKYYLPKLLLVPICHAFVYFDYIKHLKDLSSSQDDIESFEQVQG 422

Human   290 FMHQLPAFANMTMS-------------VRRELCAVMVFAVVERAGTIVL--------------ND 327
            .:|.|.......|:             |||:|       .:||...:.:              |:
  Fly   423 LLHPLHCDLEKVMASLSKERQVPVSGRVRRQL-------AIERTRELQMKVEHWEDKDVGQNCNE 480

Human   328 GEELDSWSVILNG-----SVEVTYPDGKAEILCMGNSFGVSPT----------MDKEYMKGV--- 374
            ....||.|.:.:|     ..:|...|| ..:||..|:...:|:          .:|.:|:.|
  Fly   481 FIREDSLSKLGSGKRIWSERKVFLFDG-LMVLCKANTKKQTPSAGATAYDYRLKEKYFMRRVDIN 544

Human   375 MRTKVDDCQ------------------------------FVCIAQQDYCRILNQVEKNMQK---- 405
            .|...||.:                              .:.|.:....|.|:.:.:::::
  Fly   545 DRPDSDDLKNSFELAPRMQPPIVLTAKNAQHKHDWMADLLMVITKSMLDRHLDSILQDIERKHPL 609

Human   406 -----------VEEEGEIVMVKEHRELDRTGTRKGHIVIKGTS-----ERLTMHLVEEHSVVDPT 454
                       |.:.|:.::::|...       .|..:|||.:     ||||.|:     ..|||
  Fly   610 RMPSPEIYKFAVPDSGDNIVLEERES-------AGVPMIKGATLCKLIERLTYHI-----YADPT 662

Human   455 FIEDFLLTYRTFLSSPMEVGKKLLEWFN--DPSL------------------------------- 486
            |:..||.|||.| .||.::.:.|:|.||  ||||
  Fly   663 FVRTFLTTYRYF-CSPQQLLQLLVERFNIPDPSLVYQDTGTAGAGGMGGVGGDKEHKNSHREDWK 726

Human   487 ----------RDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAK 541
                      :.:|..|:..||::||.|||.||.:   ||:..|.||      |:.       .|
  Fly   727 RYRKEYVQPVQFRVLNVLRHWVDHHFYDFEKDPML---LEKLLNFLE------HVN-------GK 775

Human   542 AKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQ 606
            :.|:                           :||||
  Fly   776 SMRK---------------------------WVDSV----------------------------- 784

Human   607 LSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVE 671
                         |.|
  Fly   785 -------------LKI------------------------------------------------- 787

Human   672 QVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTA 736
                :::.|::.|:|        |||:               ...|              |.|.
  Fly   788 ----VQRKNEQEKSN--------KKIV---------------YAYG--------------HDPP- 810

Human   737 LPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIRE 801
             |:...||..|
  Fly   811 -PIEHHLSVPN------------------------------------------------------ 820

Human   802 FAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQEL 866
                                                                             
  Fly   821 ----------------------------------------------------------------- 820

Human   867 LRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSK-TSCANLKRFEEVINQETF 930
               .:|:||.|..:|:|.||::..||:::|::|:|.:...:..:.| ....||.:..:.....|.
  Fly   821 ---DEITLLTLHPLELARQLTLLEFEMYKNVKPSELVGSPWTKKDKEVKSPNLLKIMKHTTNVTR 882

Human   931 WVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYE 995
            |:...|....|..:|:.|::..|::.:...|..|||.:.:|::.:..|.|.|||.|::.||.:|.
  Fly   883 WIEKSITEAENYEERLAIMQRAIEVMMVMLELNNFNGILSIVAAMGTASVYRLRWTFQGLPERYR 947

Human   996 KLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDG--LVNFEKL 1058
            |..::.::|.|.  ::.||:..|.|.|  ||.:|.|.....::..|.|||...:..  |:||.|.
  Fly   948 KFLEECRELSDD--HLKKYQERLRSIN--PPCVPFFGRYLTNILHLEEGNPDLLANTELINFSKR 1008

Human  1059 RMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFL 1123
            |.:|:.|                           |.:.|.......|:...|
  Fly  1009 RKVAEII---------------------------GEIQQYQNQPYCLNEEST------------- 1033

Human  1124 NAKKLYE---------DAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDK---- 1175
             .::.:|         |.||:    .||.|..|.::....:|:........:...|:||.|
  Fly  1034 -IRQFFEQLDPFNGLSDKQMS----DYLYNESLRIEPRGCKTVPKFPRKWPHIPLKSPGIKPRRQ 1093

Human  1176 ------KPVKSETSPVAPRAGSQQKAQSLPQPQ---------QQPPPAHKINQG----------- 1214
                  ..:.:.||.||..|.:...|.|:....         ...|.|...|.|
  Fly  1094 NQTNSSSKLSNSTSSVAAAAAASSTATSIATASAPSLHASSIMDAPTAAAANAGSGTLAGEQSPQ 1158

Human  1215 -----LQVPAVSLYPSRK--------------------KVPVKDLPP-------FGINSPQALKK 1247
                 ..|.|..:.|.|.                    .||...||.       ...||..|...
  Fly  1159 HNPHAFSVFAPVIIPERNTSSWSGTPQHTRTDQNNGEVSVPAPHLPKKPGAHVWANNNSTLASAS 1223

Human  1248 ILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQS----SPRKGGSGNQLRSFGSGQLDLTSS 1308
            .:.:....:|..|........||..:..:..|.||..    |||. .:||:....|..|...|.|
  Fly  1224 AMDVVFSPALPEHLPPQSLPDSNPFASDTEAPPSPLPKLVVSPRH-ETGNRSPFHGRMQNSPTHS 1287

Human  1309 SSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFD----SVPVSLHDERRQRHSVSIVETN 1369
            ::|        :.|:...|.||..|..: .....||:..    :||:|.|             .|
  Fly  1288 TAS--------TVTLTGMSTSGGEEFCA-GGFYFNSAHQGQPGAVPISPH-------------VN 1330

Human  1370 LGMG-RMERRTMIEP----DQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAAD 1429
            :.|. .||.|.:..|    .:....|.|.|::.|         .:|....|....|:.:.....
  Fly  1331 VPMATNMEYRAVPPPLPPRRKERTESCADMAQKR---------QAPDAPTLPPRDGELSPPPIP- 1385

Human  1430 SGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYN 1494
                             ..:.|......|...|      |.......:||    ::..:.|:...
  Fly  1386 -----------------PRLNHSTGISYLRQSH------GKSKEFVGNSS----LLLPNTSSIMI 1423

Human  1495 RQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVT----TEET 1555
            |:|.:.|....|.|:.:.|::            |.|             |::|.:|:    |:|
  Fly  1424 RRNSAIEKRAAATSQPNQAAA------------GPI-------------STTLVTVSQAVATDE- 1462

Human  1556 KPVPMPAHIAVASSTTKGLIARKEGRYREPPPTP------PGYIGIPITDFPEGHSHPARKPPDY 1614
             |:|:|...|.:||||..            |.||      |.....|:......::|
  Fly  1463 -PLPLPISPAASSSTTTS------------PLTPAMSPMSPNIPSHPVESTSSSYAH-------- 1506

Human  1615 NVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAP 1666
                 :.||..:......|:...|.|.|..:..| :.|..|..|.:..|.:|
  Fly  1507 -----QLRMRQQQQQQTHPAIYSQHHQHHATHLP-HHPHQHHSNPTQSRSSP 1552

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAPGEF2	NP_001338653.1	CAP_ED	37..143	CDD:237999
		cNMP	297..412	CDD:197516	30/204 (15%)
		RasGEFN	428..541	CDD:214571	46/160 (29%)
		PDZ_RapGEF2_RapGEF6-like	545..627	CDD:467237	6/81 (7%)
		RA_PDZ-GEF1	769..851	CDD:340483	0/81 (0%)
		RasGEF	874..1099	CDD:238087	61/227 (27%)
Sos	NP_476597.2	HFD_SOS1_rpt1	68..145	CDD:467039
		HFD_SOS1_rpt2	153..227	CDD:467040
		RhoGEF	249..432	CDD:238091	23/138 (17%)
		PH_SOS	476..586	CDD:269963	18/110 (16%)
		RasGEFN	637..791	CDD:214571	54/297 (18%)
		RasGEF	825..1062	CDD:238087	70/285 (25%)
		PHA03247	<1197..1502	CDD:223021	81/403 (20%)