Protein Alignment RAPGEF2 and efc25

DIOPT Version :10

• Sequence 1: NP_001338653.1
• Gene: RAPGEF2 / 9693
• HGNCID: 16854
• Length: 1685
• Species: Homo sapiens
• Sequence 2: NP_596123.1
• Gene: efc25 / 2540248
• PomBaseID: SPBC336.03
• Length: 987
• Species: Schizosaccharomyces pombe
• Alignment Length: 1097
• Identity: 224/1097 - (20%)
• Similarity: 386/1097 - (35%)
• Gaps: 299/1097 - (27%)

Human   164 MGKPPLPR-------GYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLS 221
            |.:|.|.|       |:.|..:|...|:     :.|.||.|    ..|..|..|.::.|.|.:||
pombe     1 MRRPNLDRLRLKSRQGFETSVSKPSTPS-----YSTYSLSP----TFSDKSVLSPSTMSDSYALS 56

Human   222 DIYQATES-EAGDMDLSG-LPETAVDSEDDDDEEDIERASDPLMS--RDIVRDCLEKDPI----- 277
            ..|.|..| :.|:.|..| ||..:.|.........::.:.|...|  ..:.|....:.|:
pombe    57 STYTAGSSYQNGESDYFGSLPTPSSDKSSTSPFPYLKGSFDDRFSSTHSLTRQPSPRSPLTPLKG 121

Human   278 -DRTDDDIEQLLEFMHQLPAFANMTMSVRR---ELCAVMVFAVVERAGTIVLNDGEELDSWSVIL 338
             .|...:|..:.:|......|.:|..||..   ..|::         |.:..|....|.:.|..|
pombe   122 NTRASPEIRYVSDFTPPSSPFEDMQASVHSLPLSGCSI---------GPVRTNHSSSLSNSSNSL 177

Human   339 NGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVM-----------------------RTKVD 380
            ..:.....|:..    .:.:.|...|:.|..::...:                       |..:.
pombe   178 LQTESSLRPNSS----FVNSPFFPLPSSDDLFLNDRVINLFLFYEKFSYLFTHLLSAIKSRDALS 238

Human   381 DCQFVCIAQQDYCRILNQ------VEKN-MQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSE 438
            ....|...|::...:..|      ::.| :|..|.|..|   |.|  .|:        :|...|
pombe   239 IPSLVLSLQEELFNLCQQTGTFYLLQHNLLQNFEFENPI---KLH--FDK--------IIPYFS- 289

Human   439 RLTMHLVEEHSVV--DPTF------IEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVL 495
            |||:......:.:  |.||      .||||...:.|.:
pombe   290 RLTVLTFSNRAFIFPDHTFPRLQQSAEDFLYHLQFFFT--------------------------- 327

Human   496 LWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKR-RLMTLTKPSREAPLP 559
            |..||..       .::||. .:.:.:.....||......::..:.|.| ||:....|..:..:.
pombe   328 LCANNSL-------YLSRFC-YYPSFVPNTPFGGKWTNNGLSAVSSAYRTRLLEPCLPELDKCVW 384

Human   560 FILLGGSE--KGFGIFVD---------SVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEI 613
            |:|....|  :.|..|.|         ::.|.|:.....|:..|.          :|...|.:..
pombe   385 FLLKNCDEFIENFSDFADEEYVFEICSTITSHSEQIFNKLESWDM----------SIYFDKDLSE 439

Human   614 LRNNTHLSITVKTNLFVFK----ELLT-------RLSEEKRNGAPHLP---------------KI 652
            ....|:.::   .:.||.|    :|||       .|..|..|....:|               .:
pombe   440 CEQATNFAV---QSYFVTKQRCYDLLTDLVCSSQDLMMEHSNDFSTMPTMIASIAVAFQTLFENV 501

Human   653 GDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTV----------GGRNKLKKILDKTRISILP 707
            .|..|. |.::.|   :::::...|..||.|.|||.          .|..::.:..|.|.:|.:.
pombe   502 CDFLKV-RAALVD---EMQELATKEFENKFSNANTAKDDEPARQTNKGTTRISRSSDFTAVSEMS 562

Human   708 QKPYNDIGIGQSQDDSIV---GLRQTKHIPTALPV-SGTLSSSNPDLLQSHHRILDFSATPDLPD 768
            :   :.:.:|::...||:   .|...|.:.:...| .|||.:....|:|:.|...||
pombe   563 K---DTLTLGRNSLQSILMLDNLLTNKVVQSDNNVKGGTLPALVHYLVQNVHLNKDF-------- 616

Human   769 QVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATP----DQYSLCEVS---VTPEGV--- 823
                     :.|..:.....||.:|:....:..|.....|    ..||..|..   ||.:.|
pombe   617 ---------RHSFLLTYKTFTTPQELFTLLVILFHELPPPGLDATAYSSWEKGDNFVTKKNVCTV 672

Human   824 ------------IKQRRLPDQLSKLADRIQLSGRYYLKNNM------------ETETLCSDED-- 862
                        :|.|.....:|::        |.:|::::            |.:.|.::|.
pombe   673 MNLWVQKYFFEDLKARNTLYLISEM--------RTFLRDHVVPSFHIGSVILSEIDNLWTEEPPD 729

Human   863 --AQELLRESQISLLQLSTV-----EVATQLSMRNFELFRNIEPTEYIDDLFKLR-SKTSCANLK 919
              .|.||.....:.:.|:..     |.|:|:::..|:..:.|...|:|...:..| |:::..|..
pombe   730 SLTQRLLSSPMATFISLNVYAYTPEEFASQMTLLEFDYLKQIPSREWIFRSWVSRDSRSAVRNYI 794

Human   920 RFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLR 984
            .|.   |..|:|:.:.||.:.|...|..:|..||:.|..|...:||:::.:|:|.||.||:.||.
pombe   795 NFS---NCFTYWIINCILEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPIYRLH 856

Human   985 TTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKV 1049
            ..::.:..:.......|:::.:..:|.:.||.:|....|  |.:|...||..||||:.|||...:
pombe   857 AAYKLVKAEDIICLSGLREIVETKKNFSTYRALLRKAEL--PCVPFLGVILSDLTFIDEGNPDVL 919

Human  1050 DG---LVNFEKLRMIAKEIRHVGRMAS---------------------VNMDPALMF 1082
            |.   |::|.|...:|..:..|.|..|                     ||.|.:.:|
pombe   920 DSSPHLLSFNKRHRLADVVADVCRFQSSSYEMQSNTDLQSYILHRCRFVNQDLSYLF 976

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAPGEF2	NP_001338653.1	CAP_ED	37..143	CDD:237999
		cNMP	297..412	CDD:197516	22/147 (15%)
		RasGEFN	428..541	CDD:214571	20/120 (17%)
		PDZ_RapGEF2_RapGEF6-like	545..627	CDD:467237	16/92 (17%)
		RA_PDZ-GEF1	769..851	CDD:340483	17/103 (17%)
		RasGEF	874..1099	CDD:238087	63/239 (26%)
efc25	NP_596123.1	RasGEF_N	593..700	CDD:459873	25/131 (19%)
		RasGEF	755..932	CDD:459872	54/181 (30%)