Protein Alignment RAPGEF2 and ste6

DIOPT Version :10

• Sequence 1: NP_001338653.1
• Gene: RAPGEF2 / 9693
• HGNCID: 16854
• Length: 1685
• Species: Homo sapiens
• Sequence 2: NP_588316.1
• Gene: ste6 / 2538724
• PomBaseID: SPCC1442.01
• Length: 911
• Species: Schizosaccharomyces pombe
• Alignment Length: 996
• Identity: 194/996 - (19%)
• Similarity: 343/996 - (34%)
• Gaps: 303/996 - (30%)

Human   202 VSSSHSGCSITSDSGSSSLSDIYQA-TESEAGDMDLSGLPETAVDSEDDD----DEEDIERASDP 261
            :.|...|...||...||.:.:.:.: ..|...|.:........|::...|    ::..::..:..
pombe    42 ICSEKRGWFPTSCIDSSKIQNFFSSFHSSNEKDPNAQCCAPFHVEAHLQDSAWFEKHGVQAINSI 106

Human   262 LMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLN 326
            ..|.:.:|..|:        :||..|::.:  |...|.::.|:::|...|:||. :|...::||
pombe   107 PSSEEFLRKNLQ--------NDIHHLVKGI--LTTAAAVSQSIKKEGTQVIVFG-IETVRSMVL- 159

Human   327 DGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQD 391
                  |:.:|                        :..|:|:.::..|.
pombe   160 ------SFPLI------------------------ILSTLDENFLSEVA---------------- 178

Human   392 YCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFI 456
                  ||..::..:.            ||.|.|...|.:.|:.|  :|...|..:
pombe   179 ------QVFSSLNLLP------------ELSRMGCTYGELCIRFT--KLLKQLANK--------- 214

Human   457 EDFLLTYRT-------FLSSPMEVGKKLLEW-FN----------DPSLRDKVTRVVLLWVNNHFN 503
              ||..:|.       ||.|.:......|.| ||          ..:|:..:|..|.:...:|
pombe   215 --FLFFFRPDVSFPSYFLGSLIAHEIHFLPWDFNMLCSNSVQSAHTNLQPDITSFVAILSLSH-- 275

Human   504 DFEGDPAMTRFLEEF---EN---NLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFIL 562
                        |.:   ||   |||.:|                      ||:.:.:..|..:.
pombe   276 ------------EAYHCTENEFWNLEAQK----------------------LTENTTQKVLQLVA 306

Human   563 LGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNN---------- 617
            ....|......::.:|...:.....|....:|...:.:|......|:.:|.|::.
pombe   307 EDALEAWKLDILEDIDRCIQCCRRFLSANQRINYSSSENNPFSFTSQDVEALKDELSSNLCDLYL 371

Human   618 ---------------------THLSITVKTNLFVFKELLTRLSE-------EKRNGAPHLPKIGD 654
                                 ..|.:|:|.:|...|.::.:.||       |.:......|::.|
pombe   372 WSIDLEQISPSDCLLDNYSLFVDLLVTLKVSLLRIKSIIVQFSERIVFLSLEYKFLTNIQPELND 436

Human   655 IKKASRYSIPDLAVDVEQVIG--LEKVN------------KKSKANTVGGRNKL----------- 694
            .:|:             |:.|  |.|.|            |::....:..||.|
pombe   437 AEKS-------------QLDGFDLNKTNWFDSKGLVCYLMKQTSPEPLLIRNLLFSFWSCNGKIE 488

Human   695 --KKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNP--DLLQSHH 755
              .||...|.:.|:......||       ||      |......|....::.||:.  .:|.:|.
pombe   489 QDGKIKTATLVFIINYLLRTDI-------DS------TFFTTIFLNTYASMISSSDLFSILGAHF 540

Human   756 RI---LDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQ------- 810
            |.   |:|.....:..:..||.|     |::         ::::.....:.|....:.
pombe   541 RFICSLNFGKISFISHEFYRVSK-----RFL---------DILLIWFESYLVEELDNSKSIFFLF 591

Human   811 --YSLCEVSVTPEGVIKQRRLPDQLSKL-------ADRIQLSGRYYLKNNMETETLCSDEDAQEL 866
              |.:.||.|.|. ......|...||.|       .....|.|:   :.:.|.|.|........:
pombe   592 KIYKVFEVFVVPH-FASAEELLHSLSHLLHHPSTKRSHKMLEGK---ELSQELEDLSLHNSPDPI 652

Human   867 LRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFW 931
            :.:.::.|| |...|:|.||.:..|:.|.:|...:::..::.        ||.||.......||:
pombe   653 IYKDELVLL-LPPREIAKQLCILEFQSFSHISRIQFLTKIWD--------NLNRFSPKEKTSTFY 708

Human   932 --------VASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWE 988
                    |...|::|....:|..::.:||::..:.||..||.|:|:|||.||.:|:.|||.||.
pombe   709 LSNHLVNFVTETIVQEEEPRRRTNVLAYFIQVCDYLRELNNFASLFSIISALNSSPIHRLRKTWA 773

Human   989 KLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLV 1053
            .|.:|....|:.|.:|.:..:|.:.||:.|  :|...|.:|...|...|||||..||......::
pombe   774 NLNSKTLASFELLNNLTEARKNFSNYRDCL--ENCVLPCVPFLGVYFTDLTFLKTGNKDNFQNMI 836

Human  1054 NFEKLRMIAKEIRHVGRMASV 1074
            ||:|...:.:.:..:.:..||
pombe   837 NFDKRTKVTRILNEIKKFQSV 857

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAPGEF2	NP_001338653.1	CAP_ED	37..143	CDD:237999
		cNMP	297..412	CDD:197516	16/114 (14%)
		RasGEFN	428..541	CDD:214571	26/136 (19%)
		PDZ_RapGEF2_RapGEF6-like	545..627	CDD:467237	14/112 (13%)
		RA_PDZ-GEF1	769..851	CDD:340483	16/97 (16%)
		RasGEF	874..1099	CDD:238087	64/209 (31%)
ste6	NP_588316.1	SH3	7..55	CDD:214620	4/12 (33%)
		RasGEFN	489..604	CDD:214571	26/141 (18%)
		RasGEF	659..895	CDD:214539	64/210 (30%)