Protein Alignment RAPGEF2 and pxf-1

DIOPT Version :10

• Sequence 1: NP_001338653.1
• Gene: RAPGEF2 / 9693
• HGNCID: 16854
• Length: 1685
• Species: Homo sapiens
• Sequence 2: NP_001023389.1
• Gene: pxf-1 / 177894
• WormBaseID: WBGene00004254
• Length: 1470
• Species: Caenorhabditis elegans
• Alignment Length: 1603
• Identity: 547/1603 - (34%)
• Similarity: 806/1603 - (50%)
• Gaps: 262/1603 - (16%)

Human     6 DNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDI 70
            |..|.::::|.|..|||.|:..||..|..::..|||....|:.:|:|.|||||.|..:|:...|:
 Worm    13 DARFYESLIKPPHLRTPDDIRNVYEQLRQLDTFSNLFIGPLKALCKTARYERHPAQYILFRDGDV 77

Human    71 GTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQAS 135
            ...|||||||||||:..:::|...||||:..:.||...|::|:.|||||:||..|      .|::
 Worm    78 ARSWYILLSGSVFIENQIYMPYGCFGKRTGQNHRRTHNCLLLQESEMIVIDYPTE------PQSN 136

Human   136 HRQSRRRFRKINQKGE-------RQTIID-TVDPYPMGKPP-----------------------L 169
            ....|...|.|:..||       |::..: :||...|..||                       |
 Worm   137 GMSPRTPPRGIHHSGEPVHQKTPRKSAPNMSVDSIAMPPPPVPPRPLRLPQTAAKGPAPLPPRGL 201

Human   170 PRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSH------SGCSITSDS------------- 215
            ||.|         |.||.....|.|.....|..:..|      :|.|...|:
 Worm   202 PRTY---------PLDFPVDIPTTSSSSSNTSYNDQHRSQVYLNGLSADEDTLVRVKHRREKSNS 257

Human   216 -GSSSLSDIYQAT----ESEAGDMDLSGLPETAVDSEDDDDEED-----IERASDPLMS-RDIVR 269
             |..:.:.|..|.    .|.|......|  |||.:...|.||::     .|.:|...|. ||.||
 Worm   258 VGGQAQNGISTARRLRGRSTASSTTTEG--ETASNEGADSDEDEGSMPSQESSSGGFMDLRDSVR 320

Human   270 DCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSW 334
            :||||:|.:|..:|:..||:||..:.|||.:.||::|:||..||||||..|||:||...|:||||
 Worm   321 ECLEKEPSERNSEDLAVLLDFMQHMSAFAALPMSIKRQLCLKMVFAVVNDAGTVVLAHNEKLDSW 385

Human   335 SVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQV 399
            |||:||.|||..|.|:.....:|:|||..||...:...|.|||.||||:||.:..:|:|.|::.:
 Worm   386 SVIVNGCVEVVKPSGERVEYKLGDSFGAEPTPATQIHIGEMRTMVDDCEFVLVEHRDFCSIMSTI 450

Human   400 EKNMQKVEE--EGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEE--HSVVDPTFIEDFL 460
            ..:::|..:  .||:|...|.|.:   ||..|.::|||..::|..|||:|  |: |||.:::|||
 Worm   451 GDHIEKDRDGLTGEVVSEVERRTV---GTHCGQVLIKGKPDKLIHHLVDERDHN-VDPHYVDDFL 511

Human   461 LTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLERE 525
            ||||.|:..|..:.:||:.||.|...||||.|:|||||||||||||.:..|...||.||..|||:
 Worm   512 LTYRVFIRDPTTIFEKLMLWFADSIYRDKVARLVLLWVNNHFNDFETNDEMWNLLERFEGALERD 576

Human   526 KMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKR 590
            .|...|.||||||:.|||.|.:.||: .::..:...|:||.|.|..::|..|...:.|...|:||
 Worm   577 GMHSQLSLLNIACSVKAKPRQVILTR-RKDDKMMMRLVGGQESGNSVYVAEVFPDTSAAREGVKR 640

Human   591 GDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEK------RNGAPHL 649
            .|::||||.|:.:.:...||.::|..:..|::.:|.|:..:||.:.::...|      |:|| .:
 Worm   641 ADEMLEVNQQSAKYLSAKKAEDLLTGSLSLTLMLKNNVLGYKETIGKIEHNKPKNGTSRSGA-GI 704

Human   650 PKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKIL-----------DKTRI 703
            |.:..:.|.|             :.|.:.....||:   |...||..||           |:.:|
 Worm   705 PMVIPVHKTS-------------ITGKKSSTTSSKS---GMMEKLMTILKSSKEDSMDFTDEAKI 753

Human   704 SILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDL--LQSHHRILDFSATPDL 766
            |....:|                                 |.||||:  :..::.    ....:.
 Worm   754 SSADLRP---------------------------------SRSNPDITSISQYYG----PVRSEC 781

Human   767 PDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTA--TPDQYSLCEVSVTPEGVIKQRRL 829
            |:.||::::.||..:|:.:.|:|:|:.||..|::||.:||  :| ::||||.:||.:||||||||
 Worm   782 PEHVLKIYRNDQTFKYLPVYKETSAQNVVQLALQEFNMTAEGSP-EWSLCECTVTIDGVIKQRRL 845

Human   830 PDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELF 894
            |.|:..||:||.|:.|||||||..:|.|..||.|.|||:|:|..||.|:...||.||::::|.:|
 Worm   846 PPQMENLAERIALNSRYYLKNNSRSEPLVPDELAPELLKEAQTQLLSLNAQVVAAQLTLQDFSVF 910

Human   895 RNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHC 959
            ..|||||::|:||||.||.....|:.||::.|:|.:|||:||..|.:..||.|:||.|||:|.:|
 Worm   911 SAIEPTEFLDNLFKLDSKYGSPKLEEFEQLFNREMWWVATEICTERHVQKRAKLIKKFIKVARYC 975

Human   960 RECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQ 1024
            |:.:||||||||:|||:...|.||.::||::.:||.::..::..|.||||||:|||..|.....:
 Worm   976 RDLRNFNSMFAIMSGLDKPAVRRLHSSWERVSSKYIRMLDEIHQLVDPSRNMSKYRQHLAEVAQE 1040

Human  1025 PPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKW 1089
            ||::|::|||||||||.|:||.:..:.|:||||||:|||.||.|.:::|...:.|.|..
 Worm  1041 PPVVPIYPVIKKDLTFAHDGNATYSEKLINFEKLRLIAKSIRGVMKLSSAPYEIASMAE------ 1099

Human  1090 RSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESL 1154
            ||.|.:.....:....:.:.....:|.:........||:||.|.|.||||.||..|.:..:|..|
 Worm  1100 RSGGVVMDALLHMNSFENSNVATMRKGMSGKQNQPRKKVYEQALMVRKVKSYLEGLHVVDNEMEL 1164

Human  1155 QTLSLQCEPATNTLPK--NPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKI---NQG 1214
            .::|...||...|..:  |......::...|| .|.:.|.|.|.|..|..:     |::   ..|
 Worm  1165 DSMSYDIEPQVQTAHRGANSSSTANIRRVPSP-TPSSLSSQSAGSADQSSR-----HRLLFNGTG 1223

Human  1215 LQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPP 1279
                ::|......|        ||:.||||::|:|||.:...           :..|..|::||.
 Worm  1224 ----SISSAGGGSK--------FGVESPQAVQKMLSLVQNSK-----------VKGAPPQITSPS 1265

Human  1280 TSPQSS-----PRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSS 1339
            ||.:||     ||.  :|.|..|...|.:.|...:|::.:|..         ||:|..:.|.
 Worm  1266 TSARSSLQRNMPRV--TGRQATSSAQGPVQLNEETSTVTTYYQ---------SDNGRRQRSG--- 1316

Human  1340 IVSNSSFDSVPVS---LHDER---RQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGR 1398
              |...||::|.|   |..:.   ..|.|:|:|......|.       .|......|.:|.|.|.
 Worm  1317 --SEGRFDNIPPSTFYLTSDGLTVSPRQSLSVVIPTHPHGH-------SPTSPRCRSRSPASSGC 1372

Human  1399 GLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQ-HQRSWETLPF-- 1460
            ..::|...|::             .|:.||.|      :..|..:.:.||:: |......:|.
 Worm  1373 SSFSTIASIAA-------------TSMAAAPS------AFVSNPYQHHQTVRGHVIGHRPMPIVT 1418

Human  1461 -GHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRES 1502
             |.........|.||...|  ...|:...|:...:|....:|:
 Worm  1419 SGSATLPNHVSPRGLPPKS--RPTILPGSHTNSSSRMGTIKEA 1459

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RAPGEF2	NP_001338653.1	CAP_ED	37..143	CDD:237999	44/105 (42%)
		cNMP	297..412	CDD:197516	54/116 (47%)
		RasGEFN	428..541	CDD:214571	61/114 (54%)
		PDZ_RapGEF2_RapGEF6-like	545..627	CDD:467237	25/81 (31%)
		RA_PDZ-GEF1	769..851	CDD:340483	42/83 (51%)
		RasGEF	874..1099	CDD:238087	106/224 (47%)
pxf-1	NP_001023389.1	CAP_ED	45..131	CDD:237999	41/85 (48%)
		cNMP	348..458	CDD:197516	53/109 (49%)
		RasGEFN	479..592	CDD:214571	61/113 (54%)
		PDZ_canonical	596..662	CDD:483948	22/66 (33%)
		RA_PDZ-GEF1	784..867	CDD:340483	42/83 (51%)
		RasGEF	890..1118	CDD:238087	106/233 (45%)