Protein Alignment EIF2AK3 and PEK

DIOPT Version :10

• Sequence 1: NP_004827.4
• Gene: EIF2AK3 / 9451
• HGNCID: 3255
• Length: 1116
• Species: Homo sapiens
• Sequence 2: NP_649538.1
• Gene: PEK / 40653
• FlyBaseID: FBgn0037327
• Length: 1162
• Species: Drosophila melanogaster
• Alignment Length: 1217
• Identity: 399/1217 - (32%)
• Similarity: 582/1217 - (47%)
• Gaps: 258/1217 - (21%)

Human    62 AGAVA--AAEV----TVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDP 120
            ||.||  .|:|    ...|:|.  ..||...|.|.|...:.|.|...|.|:.|||||||::|||.
  Fly    27 AGLVAFDPAQVLAGHPTTDSEL--QTAGSPRPPGLEHCVDQEERRVARRLLYISTLDGRLSALDI 89

Human   121 ENHGKKQWDLDVGSGSLVSSSLSKPEVFGNKM---IIPSLDGALFQWDQDRESMETVPFTVESLL 182
            ...||.:|.:..|.|.|:|||:.:.|:..|..   :||||.|.::::|.|  |::.:|.|.|.||
  Fly    90 AKSGKLRWSVPTGPGPLISSSIHRLELTNNGQFVRMIPSLSGGIYKFDGD--SIDPIPITAEHLL 152

Human   183 ESSYKFGDDVVLVGGKSLTTYGLSAYSGKVRYICSALGC--------------RQWD-SDEMEQE 232
            .||.||.||:|:.|||...:||:|..:|::.|.||..||              |:.| .|::|..
  Fly   153 SSSAKFSDDLVISGGKETRSYGVSVRTGQLLYECSLNGCVNSTEEGLAIDDTIREPDEEDQLEDG 217

Human   233 E-----------------DILLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRAGFIE 280
            |                 |:::::|..:|||||..|:|.|:||||||..||..:
  Fly   218 EQLRDEAGYIVRHDPLLDDVIIVRRQTQTVRAVESRTGVERWNFSVGQHELDLV----------- 271

Human   281 STFKPNENTEESKIISDVEEQEAAIMDIVIKVSVADWKVMAFSKKGGH-LEWEYQFCTPIASAWL 344
               :|:|...:.:     :|.|.|::|:.|||.|.:..:.||||.... :.|:|:|..||.|||.
  Fly   272 ---RPSECQLQPR-----DELELAVLDVDIKVVVPEGIICAFSKSEPQTMLWKYKFDHPIVSAWN 328

Human   345 LK-DGKVIPISLFDDTSYTSNDDVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFP 408
            .. |.::.||.||....:..:.|  |::.::..|.:  :..|:|||||..|||:|.|:|:.::..
  Fly   329 TNADDELQPIDLFSSAQWLWDQD--ENDTELPNAPQ--SPPSIYLGMYDKQLYIQESIRLRQEIM 389

Human   409 SSPKALESVTNENAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSND----------KFSHEE 463
            ...|..:.:|.:.::  :|.|.||| |.:.|::.|:...|:.|..:..:          .:..|.
  Fly   390 DQTKVYQQLTGDTSL--MPRIPWKP-ISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDEN 451

Human   464 YSNGALSIL---QYPYDNGYYL--------------------------PYYKRERNKRSTQITVR 499
            ::..|.|:|   ::...||:|.                          |..........|:.|..
  Fly   452 FAVAAQSVLNASEFVNGNGFYFYTTGDLNGPQECSTQNNPTDLPAITAPTSPTNATSEGTEATGN 516

Human   500 FLDNPHYNKNIRKKD-PV-----LLLHWWKEIVATILFCIIATTFIVRRLFHPHPHRQRKESE-- 556
            ...|.....::...| ||     .|..||||||.     |..|:.::..:|... ..||.|.|
  Fly   517 HSVNDDLGFSLDDIDAPVKVVILSLWFWWKEIVV-----IAFTSAVILNIFMGQ-RNQRVEREYL 575

Human   557 --------------TQCQTE-------------NKYDSVSGEANDSSWND-IKNSG--YISRYLT 591
                          |:..|:             |::....|:||..:.:: ..:||  |.||:.:
  Fly   576 VIERHVPVQTAIEATEASTQALLGPVVPMQRPGNRFSFPPGQANQRTISESTTHSGEHYTSRFQS 640

Human   592 DFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFN 656
            |||.:|||||||||||||||||:|:..||||||.|||:|.:|::|:||.:.||..||..|||||:
  Fly   641 DFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFH 705

Human   657 AWLEAPPEKWQEKMDEIWLKDE-STDWPLSSPSPMDAPS----VKIRRMDPFATKEHIEIIAPSP 716
            :|.|.||..|||:.|...|..| ||...:.:|.....||    :|.:|.....:  .:...|.|.
  Fly   706 SWTETPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLS--WVSDAANST 768

Human   717 QRSRSFSV-GISC-----DQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCDV-EDG 774
            ..|..|.: |.|.     ::....|.:.|.:||.       |||..||...::...||.|. ||.
  Fly   769 ACSHDFHLPGESSLKNIREEYDYDEEEDSLIEFR-------SESQSAALRAEEEDDTDDDYEEDE 826

Human   775 TMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNAS---SKEEPKTNRLHIGNHCANK 836
            ...|:.|                .|.|:|.||....:..|..:   |:.:..:|...|
  Fly   827 EQQGDHE----------------KRHRSSVSIDIHSASFDLKNINYSQHQLVSNSFQI------- 868

Human   837 LTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSP--------------KVYLYIQ 887
             .:.:|.||.|......:.:..:|.||:|....|..:.....:|              |||||||
  Fly   869 -ESVRPKSSGSDDANDDNKARRKPLTLALAQNHNNNQNGSQPTPSSATILNGTVAKPSKVYLYIQ 932

Human   888 MQLCRKENLKDWMNGRCTIEERERSVCLH---IFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDD 949
            |||||||:|:||:.     :.|..:...|   ||.||.:||:::|.|||:|||||||||||:.|.
  Fly   933 MQLCRKESLRDWLR-----DNRSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDG 992

Human   950 VVKVGDFGLVTAM-----------DQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSH 1003
            .:|:|||||||.|           ||        :.:|:.||||.||||.|||||||:.|..|.:
  Fly   993 QIKIGDFGLVTDMADIPNLVAKCGDQ--------SGLPSCARHTQQVGTHLYMSPEQLLGQHYDY 1049

Human  1004 KVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEA 1068
            ||||:|||||.|||...|||:|||::||..:|:.::|..|...||.:|.::|.|||..|.:||:.
  Fly  1050 KVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQT 1114

Human  1069 -------------INIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKH 1102
                         .:::.....|..:...:.....|||:.|||...|
  Fly  1115 KQLKSQLRNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPH 1161

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EIF2AK3	NP_004827.4	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	77..101		7/23 (30%)
		Luminal_EIF2AK3	104..420	CDD:188874	121/352 (34%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	550..571		7/49 (14%)
		Protein Kinases, catalytic domain	587..>663	CDD:473864	48/75 (64%)
		Insert loop. /evidence=ECO:0000250|UniProtKB:Q9Z2B5	647..888		71/269 (26%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	841..863		5/21 (24%)
		STKc_EIF2AK3_PERK	<873..1075	CDD:270950	104/242 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1090..1116		7/13 (54%)
PEK	NP_649538.1	Luminal_EIF2AK3	73..401	CDD:188874	121/352 (34%)
		Protein Kinases, catalytic domain	635..1113	CDD:473864	206/523 (39%)