Protein Alignment MAP4K4 and msn

DIOPT Version :10

• Sequence 1: NP_001381931.1
• Gene: MAP4K4 / 9448
• HGNCID: 6866
• Length: 1384
• Species: Homo sapiens
• Sequence 2: NP_524679.3
• Gene: msn / 44030
• FlyBaseID: FBgn0010909
• Length: 1504
• Species: Drosophila melanogaster
• Alignment Length: 1624
• Identity: 714/1624 - (43%)
• Similarity: 894/1624 - (55%)
• Gaps: 383/1624 - (23%)

Human     9 SLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINML 73
            ||.||||::|:||||||||:||||||||||||||||.||||||||||||||||||||||||||:|
  Fly    16 SLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVL 80

Human    74 KKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREIL 138
            ||||:||||||||||||||||||.|||||||||:|||||:|||||:|||.:|||:|||||.||||
  Fly    81 KKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREIL 145

Human   139 RGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACD 203
            |||::||.:.||||||||||||||:||||||||||||||||||:|||||||||||||||||||||
  Fly   146 RGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACD 210

Human   204 ENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEG 268
            ||||||||.||||||.||||:||||..|||||:||||||||||||.|||||||||||||..||:.
  Fly   211 ENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDT 275

Human   269 CLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEV 333
            .|||:|.|||.||.||||.||:|||.:|||||||||||||.:|::.||:..:|.||||:.:::|
  Fly   276 VLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDE- 339

Human   334 PEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKR 398
            |:..||.||||..||..||||:|                 |.:||.:|..:::.:..|:|:.|
  Fly   340 PQLAGEHSSIVQAPGGDTLRRNF-----------------QQIQEGRLAAEQQQQHHLMAQAQ-- 385

Human   399 IEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVER 463
                         .|.....|..|.:.:
  Fly   386 -------------AQAAAAHAAAQAQAQ------------------------------------- 400

Human   464 EQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDH 528
                              ||||  |:||...........:..|....|.|.||.||        :
  Fly   401 ------------------LQQQ--QQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQA--------N 437

Human   529 RRPHPQHSQQ-----------------PPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKT 576
            |:|.|...||                 |.||...|..|    |.||.. |||.:..:.:.|..:.
  Fly   438 RQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLP----PTPKCG-EPAGQTPQQQQRNSQN 497

Human   577 NHS------SPEAQ---------SKQTGRVLEPPVPSRSES--FSNGNSESVHPALQRPAE--PQ 622
            |..      .||.|         |:|..:...||..:|..|  .|:|.|.|......:||.  ||
  Fly   498 NFKPSLPPRRPEPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGLSSSGGSASGGGGSSKPAAALPQ 562

Human   623 VQWSHLASLKNNVSPVSRSHSFSDP-SPKFAHHH---LRSQDP---------------------- 661
            ...:||....|.:.|:..|.|.|:| .|.....:   |.:.||
  Fly   563 QSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDANTTTPLSH 627

Human   662 ---CPPSRSEVLSQSSDSKSEAPDPT--------------QKAWSRSDSDEVPPRVPVRTTSRSP 709
               .||:|.  |..:.|...:|.|.|              ||:.|.|.::..|.    |....|.
  Fly   628 GSGGPPNRP--LPPTPDDDDQAGDRTLIMKRKLEQNINRLQKSASTSQANVTPS----RRGDESN 686

Human   710 VLSRRD----SPLQGSGQQNSQAGQRNSTSS-----------IEPRL----LWERVEKLVPR--- 752
            :|...|    .|...:|.:.|..|...:|:|           ::.:|    :.|.:.:.|||
  Fly   687 LLRDWDFDRFFPKNANGPRGSGRGSPTTTASLSRSSQLSTLKVDTKLQRASVAEAITRPVPRGYQ 751

Human   753 PGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPS----QRLENAVKKPEDKKEVFR 813
            |.....|.|.:...:.||..||.||||.    .|.|.|.||.:    :|.::..:.|.:.:..||
  Fly   752 PLKAEPSASQSIAKEQGSASGSGSGSGS----ASGSGSGGSTNSPAHKRQDSDSRLPTNFERGFR 812

Human   814 PLKPAGEVDLTALAKELRAV----------------EDVRPPHKVTDYSSSSEESGTTDEEDDDV 862
                ....|...|||...||                :.::....|...:|....|.::..:::||
  Fly   813 ----RENSDFFPLAKRYSAVFSGASASGSTAGSPSAQALQRSSAVYQRNSIYSSSISSKSKENDV 873

Human   863 E----QEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGT--LIV--- 918
            .    ...|..:|:..:...||::...:.|........:.:...     :.|.:|.|  :||
  Fly   874 PGAPGAAAAGTATASAKPGPAAATTTATKGAAPKTSKSLANFHF-----LRPRREKTESVIVLQN 933

Human   919 ---------------RQSTVDQKRASHHESNGFAG-------------------------RIHLL 943
                           :|..:.|::...:...|..|                         ...:|
  Fly   934 AAVRAQRLQQLQQQQQQQQLQQQQQQQNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVL 998

Human   944 PDLLQQS-------HSSS---------TSSTSSSPSSS--------------------------- 965
            ||||.|:       |..|         :||..|:||.|
  Fly   999 PDLLSQASPATPPRHDKSSSEEYQAAISSSVHSTPSKSFIASSGSGGLGLGGGTVVGGVIISSNH 1063

Human   966 QPTPTMS---------PQTPQDKLTANETQSASS-TLQKHKSSSSFTPFIDPRLLQISPSSGTTV 1020
            .|..|:|         ..:|::.:  ::|:|:|| |...|||:||.:    ..|..::|.|.|:.
  Fly  1064 SPQSTISLASSSSNSRQNSPKNSI--SKTRSSSSITNLLHKSASSSS----ANLHHLTPCSSTSS 1122

Human  1021 TSV--------------------VGFSCDGMRPEAIRQDPTRKGSVVNVN--PTNTRPQSDTPEI 1063
            .|:                    .||...|       ..|:|:.|.||||  ||:....:|||||
  Fly  1123 ASISNPLPPHAYALQQKQRSFLTFGFGAGG-------SGPSRRESHVNVNVTPTSHEAANDTPEI 1180

Human  1064 RKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTIS 1128
            ||||||||||||||||||||||:|||:||||||||||||||.||:|||||||:||||.|:|||||
  Fly  1181 RKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTIS 1245

Human  1129 GKKDKLRVYYLSWLRNKILHND---PEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSV 1190
            |||:::||||||||::|||..|   .:||::.||..||||:|.||:|:||||||||||||||.|:
  Fly  1246 GKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIVKYERIKFLVIALKDSI 1310

Human  1191 EVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLP 1255
            |:|||||||||||||||:||||.|:|||||||:|:..||||||||..||||||:||..|||||||
  Fly  1311 EIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAVDLDSAEVYDIYLP 1375

Human  1256 THIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMG 1320
            .|.|.:|.||.|:.|||::||:||:||::|||||||.||::|::||||||||||||||.:.|.||
  Fly  1376 KHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMG 1440

Human  1321 WGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW 1384
            ||.|||||||||:||||||||||:||||||||||||||||:|.:...|.|:|||||.:..:.:|
  Fly  1441 WGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW 1504

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MAP4K4	NP_001381931.1	Protein Kinases, catalytic domain	8..289	CDD:473864	239/279 (86%)
		CNH	1066..1364	CDD:214481	230/300 (77%)
msn	NP_524679.3	STKc_myosinIII_N_like	25..296	CDD:270785	233/270 (86%)
		CNH	1183..1484	CDD:214481	230/300 (77%)