Protein Alignment RHOT2 and GEM1

DIOPT Version :10

• Sequence 1: NP_001339204.1
• Gene: RHOT2 / 89941
• HGNCID: 21169
• Length: 619
• Species: Homo sapiens
• Sequence 2: NP_009351.1
• Gene: GEM1 / 851249
• SGDID: S000000046
• Length: 662
• Species: Saccharomyces cerevisiae
• Alignment Length: 650
• Identity: 204/650 - (31%)
• Similarity: 324/650 - (49%)
• Gaps: 110/650 - (16%)

Human     2 RRDVRILLLGEAQVGKTSLILSLVGEEF----PEEVPPRAEEITIPADV--TPEKVP--THIVDY 58
            :..:|:::.|:..|||:|||:||...||    .:.:||    |:||.|.  :|...|  |.::|.
Yeast     3 KETIRVVICGDEGVGKSSLIVSLTKAEFIPTIQDVLPP----ISIPRDFSSSPTYSPKNTVLIDT 63

Human    59 SEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKSDLR 123
            |:::..  .|..|:..|:|:.:||  .:..:.:.:...|:|...   :.|..:|:||..||.|..
Yeast    64 SDSDLI--ALDHELKSADVIWLVY--CDHESYDHVSLFWLPHFR---SLGLNIPVILCKNKCDSI 121

Human   124 SGSSMEAV-------------------LPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHP 169
            |..:..|:                   :||:.:|.||:||::.|||...::::.||..|:|:.||
Yeast   122 SNVNANAMVVSENSDDDIDTKVEDEEFIPILMEFKEIDTCIKTSAKTQFDLNQAFYLCQRAITHP 186

Human   170 TAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQQKSCFGHPLAPQALEDVKTVV 234
            .:||:|....:|:|....||.|||.|||.:.|..|.|.|:...|:| ||...:....|..:|.::
Yeast   187 ISPLFDAMVGELKPLAVMALKRIFLLSDLNQDSYLDDNEILGLQKK-CFNKSIDVNELNFIKDLL 250

Human   235 CRNVAGGVREDR-------------LTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDALELTAD 286
            . :::   :.|:             :|.||||.||.::.:||||||||.|||.|.|:|:|.:...
Yeast   251 L-DIS---KHDQEYINRKLYVPGKGITKDGFLVLNKIYAERGRHETTWAILRTFHYTDSLCINDK 311

Human   287 YLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAP--W-GPELP- 347
            .|.|.:.||...|.||:..||:|:..:|.|.|.|.||.|:..||..||...|..|  | ....|
Yeast   312 ILHPRLVVPDTSSVELSPKGYRFLVDIFLKFDIDNDGGLNNQELHRLFKCTPGLPKLWTSTNFPF 376

Human   348 RTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQ 412
            .||....|.:.|.|:|.||::.|:|:..:...:|.|.|:    ::|...|:.||:.:::.:..|:
Yeast   377 STVVNNKGCITLQGWLAQWSMTTFLNYSTTTAYLVYFGF----QEDARLALQVTKPRKMRRRSGK 437

Human   413 TQRS------VLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQV-NGQEKYLIL 470
            ..||      |..|.|:|....|||:.|:|||||....:.:....|..|:::::: .|::.||||
Yeast   438 LYRSNINDRKVFNCFVIGKPCCGKSSLLEAFLGRSFSEEYSPTIKPRIAVNSLELKGGKQYYLIL 502

Human   471 CEVG-TDGLLATSLD--ATCDVACLMFDGSDPKSFAHCASVYKH--HYMDGQTPCLFVSSKADLP 530
            .|:| .:..:..:.|  ..|||.||.:|.|||:||::..|:...  |..|  .|.:||:|||||.
Yeast   503 QELGEQEYAILENKDKLKECDVICLTYDSSDPESFSYLVSLLDKFTHLQD--LPLVFVASKADLD 565

Human   531 EGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGL 595
            :        ..:.|:            ..|.|.:..:|            |:..||.||.||..|
Yeast   566 K--------QQQRCQ------------IQPDELADELF------------VNHPLHISSRWLSSL 598

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RHOT2	NP_001339204.1	Miro1	3..169	CDD:206680	54/192 (28%)
		EF_assoc_2	221..304	CDD:462444	32/95 (34%)
		EF_assoc_1	342..409	CDD:462443	19/68 (28%)
		Miro2	414..590	CDD:206679	55/187 (29%)
GEM1	NP_009351.1	P-loop containing Nucleoside Triphosphate Hydrolases	6..186	CDD:476819	54/190 (28%)
		EF_assoc_2	236..329	CDD:462444	32/96 (33%)
		EF_assoc_1	369..434	CDD:462443	19/68 (28%)
		Miro2	445..624	CDD:206679	56/187 (30%)