Protein Alignment RHOT2 and gem1

DIOPT Version :10

• Sequence 1: NP_001339204.1
• Gene: RHOT2 / 89941
• HGNCID: 21169
• Length: 619
• Species: Homo sapiens
• Sequence 2: NP_587725.1
• Gene: gem1 / 2538910
• PomBaseID: SPCC320.04C
• Length: 630
• Species: Schizosaccharomyces pombe
• Alignment Length: 639
• Identity: 224/639 - (35%)
• Similarity: 353/639 - (55%)
• Gaps: 37/639 - (5%)

Human     3 RDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADV-TPEKVPTHIVDYSEAEQTDE 66
            ::||:::.|:..|||:|||.:|:.|:....:|.....|:||::. :.:.|...:||........|
pombe     2 KEVRVVICGDQGVGKSSLISALIQEDNVTSIPKVFPIISIPSNPDSNDDVSLVLVDTQSDSNERE 66

Human    67 ELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKS-DLRSGSSMEA 130
            .|..||.||||:|:||  |:..:.|::...|:|...   :.|..|||:|..||| ||.:...:..
pombe    67 YLAAEIKKANVICLVY--SDNYSYERVSIFWLPYFR---SLGVNVPIVLCENKSEDLDNYQGLHT 126

Human   131 V----LPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTR 191
            :    :|::::|.|||.|:.|||....|::||||..:..|::|..||:|.:.:.:|.|...||:|
pombe   127 IEHEMIPLINEFKEIEACILCSALEKINVNELFYMCRACVIYPITPLWDAKERTMRKATIHALSR 191

Human   192 IFRLSDQDLDQALSDEELNAFQQKSCFGHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLN 256
            ||.|.|::.|..||.:|||:..:| ||...|:.:...::.:.|......||.|.:|||.|||..|
pombe   192 IFFLIDKNNDDLLSVDELNSLSEK-CFSKNLSIEDASEILSKVKEICPEGVYEGQLTLPGFLAYN 255

Human   257 TLFIQRGRHETTWTILRRFGYSDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDR 321
            .:.::.|:.|:||.|||.|.|:|:|.|...||||...|.||...||:..||:|:..:|.:.|:|.
pombe   256 RVQVENGKQESTWGILRAFHYTDSLSLDDSYLSPKFEVAPGQIVELSPKGYRFLVDLFYQFDRDN 320

Human   322 DGALSPVELQSLFSVFPAAP--W-GPELPR-TVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLG 382
            ||||:..||.:||...|..|  | ..:.|. ||..|.|.:..:|:|.||:::|..|.::.|.:|.
pombe   321 DGALNNEELSALFRHTPGLPEIWVSSQFPNSTVLNEHGYVTYNGWLAQWSMITLFDYKTTLAYLA 385

Human   383 YLGYPTLCEQDQAHAITVTREKRLDQEK--GQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQ 445
            |||:.|........|:.|.| ||:.|.:  .:..|:|.||.|||::..||:|.|.:|:     :.
pombe   386 YLGFDTDGRGHNTDALKVMR-KRVSQNRKVSKYDRNVFLCFVVGSKSCGKTALLSSFI-----NN 444

Human   446 DTREQPPGYAIDTVQVNGQEKYLILCEVG-TD-GLLA--TSLDATCDVACLMFDGSDPKSFAHCA 506
            :|....|...:::|:....::||:|.|:| || .:||  .||:| ||:.||::|.|:|.||:..|
pombe   445 NTNRLTPNTVVNSVEFQSTQRYLVLSEIGETDLDILAEPKSLEA-CDILCLLYDSSNPNSFSFIA 508

Human   507 SVYKHHYMDGQ-TPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQ 570
            ::. :.|.|.| .||:|.::||||..........|.||.::..||:|...|.|.....|...|.|
pombe   509 NLL-NLYPDLQKIPCVFAATKADLDRQQQRYPVQPDEFTKQLGLPSPTHISTAAIWNTSKEFFIQ 572

Human   571 LATMAAFP-----HLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ 619
            ||..|.:|     .:...:.:.:::.|...|...||.:.:| ..||...::|.|
pombe   573 LAESAQYPASSIIRIPEEDSNKTNYQLVAALTAFGALLLSV-GGSLTWKIIKHQ 625

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RHOT2	NP_001339204.1	Miro1	3..169	CDD:206680	58/171 (34%)
		EF_assoc_2	221..304	CDD:462444	33/82 (40%)
		EF_assoc_1	342..409	CDD:462443	23/68 (34%)
		Miro2	414..590	CDD:206679	62/185 (34%)
gem1	NP_587725.1	Miro1	2..169	CDD:206680	58/171 (34%)
		EF_assoc_2	219..303	CDD:462444	33/83 (40%)
		EF-hand_5	309..332	CDD:433035	9/22 (41%)
		EF_assoc_1	343..412	CDD:462443	24/69 (35%)
		Miro2	418..595	CDD:206679	62/183 (34%)