Protein Alignment EIF4G3 and tif471

DIOPT Version :10

• Sequence 1: XP_047289187.1
• Gene: EIF4G3 / 8672
• HGNCID: 3298
• Length: 1822
• Species: Homo sapiens
• Sequence 2: NP_594602.1
• Gene: tif471 / 2542290
• PomBaseID: SPAC17C9.03
• Length: 1403
• Species: Schizosaccharomyces pombe
• Alignment Length: 1528
• Identity: 355/1528 - (23%)
• Similarity: 540/1528 - (35%)
• Gaps: 438/1528 - (28%)

Human     2 SLPQKPALKSGTATAAGTGPGTGAAAAAAAAVP--PPHPAAAAAAAAAAAAAAAAAVPPPHPNIR 64
            ::|.||.:.......:|....:..:.:..::|.  |||.....||...|::.|...   |.|...
pombe   175 AIPSKPKVSGNMQNKSGVSSYSSKSQSVNSSVTSNPPHTEEPVAAKPEASSTATKG---PRPTTS 236

Human    65 ALQTQ-APQQIPRGPVQQPLEDRIFT-PAVSAVYSTVTQVARQPGTPTPSPYSAHEINKGHPNLA 127
            |..|. :|..  ..|..:|..|...| ||......:.:......|:.|||..|:....:.|.|.:
pombe   237 ASNTNTSPAN--GAPTNKPSTDINTTDPATQTTQVSASNSPALSGSSTPSNTSSRSNRQNHGNFS 299

Human   128 ATPPGHASSPGLSQTPYPSGQNAGPTTLVYPQTPQTMNSQPQTRSPPSRTVPIHCTDNWKRRKVL 192
                        .:..|....|:.|:...|.......|   ...|..:|....|......||...
pombe   300 ------------EKRHYDRYGNSHPSYNKYSHYQHGFN---YNNSGNNRNESGHPRFRNSRRNYN 349

Human   193 EQ--TPVYRSLAGRGWIKYCIFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHL 255
            .|  .|.|.|..            |...|.||    |:..::..|: ||.             .:
pombe   350 NQGAYPTYMSNG------------RSANQSPR----NNPQNVNNGS-TPI-------------QI 384

Human   256 PMPYPVPQGPQYCIPQYRHSGPPYVGPPQ-QY-PVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQP 318
            |:....|.|..|..|||      .|.|.. || |:..||..|.|                   .|
pombe   385 PVSLQTPYGQVYGQPQY------IVDPNMVQYGPILQPGYVPQY-------------------YP 424

Human   319 VYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQ 383
            ||...|.   ||..|             :.::.|..::::::....|...:|..|
pombe   425 VYHQTPY---TQNFP-------------NMSRSGSQVSDQVVESPNSSTLSPRNG---------- 463

Human   384 LPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEG 448
                   .:|:|....:|:.|....|||      ..|...|.....||:|
pombe   464 -------FAPIVKQQKKSSALKIVNPVT------HTEVVVPQKNASSPNP--------------- 500

Human   449 QTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPI 513
              |||.   |.||.|              |.|.|..|.               |.:|.|:|.|.:
pombe   501 --SETN---SRAETP--------------TAAPPQISE---------------EEASQRKDAIKL 531

Human   514 PSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLVSSTNLINEINGVSE-KLSATESIVEIVK 577
            .......|.::.....:.::                  ...|..|.|...| :..|.....|..|
pombe   532 AIQQRIQEKAEAEAKRKAEE------------------KARLEAEENAKREAEEQAKREAEEKAK 578

Human   578 QEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSP 642
            :|.     |.:......|:.|.|                                   |...:..
pombe   579 REA-----EEKAKREAEEKAKRE-----------------------------------AEENAKR 603

Human   643 SAAITVQRVLEE------DESIRTCLSEDAK-EIQNKIEVEADGQTEEILDSQNLNSRRSPVPAQ 700
            .|....:|..||      :|..:....|.|| |.:.|.:.||:.:.:.  :::....|.:...|:
pombe   604 EAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKR--EAEEKAKREAEENAK 666

Human   701 IAITVPKTWKKPKDRTRTTEEMLEAELE----LKAEEE--LSIDK---------VLESEQD---- 746
            .........:..::..|..||.::.|.|    .|||||  ...||         :..||.:
pombe   667 REAEEKAKREAEENAKREAEEKVKRETEENAKRKAEEEGKREADKNPEIKSSAPLASSEANVDTS 731

Human   747 KMSQGFHPE-RDPSDLKKVKAVEENGEEAEPVRNGAESVSEG----EGIDANSGSTDSSGD---- 802
            |.:....|| .|.:.::|:||.|  |:....:.:.:.|.|..    .|:::.|..|:...:
pombe   732 KQTNATEPEVVDKTKVEKLKASE--GKSTSSLSSPSHSTSSKRDLLSGLESLSLKTNPKSEQCLE 794

Human   803 ------------GVTFP--FKPESWKPTDTEGKKQYDREFLLDFQFMPACIQKPEGLPPISDVVL 853
                        .:.:|  .||.|.:.....||.:||..|||.||                .|..
pombe   795 SLLNSQFITDFSALVYPSTIKPPSTEEALKAGKYEYDVPFLLQFQ----------------SVYT 843

Human   854 DKINQPKLPMRTLDPRILPRGPDFTPAFAD--------FGRQT------------PGGRGVPICK 898
            ||      ||:..|.|:   ......||:|        ..||:            ||..|....|
pombe   844 DK------PMKGWDERM---KETVASAFSDKSSRGMYSSSRQSSRSGSNTHSHAGPGFGGPSERK 899

Human   899 VQSRHGLP---------ILEQSKAPTCPPLVMSHPPMKSLPLGLLNVGSRRSQPGQRR------- 947
            ..||.|:.         ....|...:.|...:||...     |.::...|.||.|.||
pombe   900 GISRLGIDRGFSSSGAGFGSGSNYKSAPSRGVSHHGH-----GGMSGSHRGSQRGSRRGGGERDK 959

Human   948 -EPRKIITVSVKEDVHLKKAENAWKP----SQKRDSQADDPENIKTQELF-RKVRSILNKLTPQM 1006
             :|.. :|:.|.:...|:.:.|.|:|    .:..:::.:|.|.:...|:. |||:..|||:|.:.
pombe   960 PDPSS-LTIPVDQVAPLQLSANRWQPKKLTEKPAETKGEDEEALLPPEVVQRKVKGSLNKMTLEK 1023

Human  1007 FNQLMKQVSGLTVDTEER-----LKGVIDLVFEKAIDEPSFSVAYANMCRCLV------TLKVPM 1060
            |:::..|:..:.:.:.:.     ||.||.|.||||.|||:||..||...|.::      .....:
pombe  1024 FDKISDQILEIAMQSRKENDGRTLKQVIQLTFEKATDEPNFSNMYARFARKMMDSIDDSIRDEGV 1088

Human  1061 ADKPGNTVN----FRKLLLNRCQKEFEKD-KADDDVFEKKQKELEAASAPEERTRLHDELEEAKD 1120
            .||....|.    |||.||:|||::||:. ||:          |.:..|.|... :.||...|.
pombe  1089 LDKNNQPVRGGLLFRKYLLSRCQEDFERGWKAN----------LPSGKAGEAEI-MSDEYYVAA- 1141

Human  1121 KARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN---HDEESLECLCRLLTTIGKDLD-FEK 1181
            ..:||.:|.::||||||||.||:|.|||:|:.:||.|   .:||.:|.|||||.|:|.::| .||
pombe  1142 AIKRRGLGLVRFIGELFKLSMLSEKIMHECIKRLLGNVTDPEEEEIESLCRLLMTVGVNIDATEK 1206

Human  1182 AKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNW-VSRRADQGPKTIEQIHKEAKIEEQ 1245
            ....||.|..:||.|.|.....|||:|||.||:|.|...| |....::|||||.:||:||
pombe  1207 GHAAMDVYVLRMETITKIPNLPSRIKFMLMDVMDSRKNGWAVKNEVEKGPKTIAEIHEEA----- 1266

Human  1246 EEQRKVQQLMTKEKRRPGVQRV---DEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKA 1307
             |::|.    ..|.:||...|:   |....::..|.:.|.                      |..
pombe  1267 -ERKKA----LAESQRPSSGRMHGRDMNRGDSRMGGRGSN----------------------PPF 1304

Human  1308 QLGSWGKGSSGGAKASE-TDALRSSASSLNRFSALQPPAPSGSTPSTPVEFDSRRTLTSRGSMGR 1371
            ....|.....|.|:..: ...|:|.....:..::|......||...||    ||:    ..::.|
pombe  1305 SSSDWSNNKDGYARLGQGIRGLKSGTQGSHGPTSLSSMLKGGSVSRTP----SRQ----NSALRR 1361

Human  1372 EKNDKPLPSATARPNTFMRGGSSKDLLDNQSQE 1404
            |::.:..||     |..:...:|.:||:....:
pombe  1362 EQSVRAPPS-----NVAVTSANSFELLEEHDHD 1389

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EIF4G3	XP_047289187.1	PHA03378	<74..492	CDD:223065	85/422 (20%)
		MIF4G	992..1220	CDD:397130	98/248 (40%)
		MA3	1459..1571	CDD:397128
		W2_eIF4G1_like	1665..1793	CDD:211397
		W2	<1772..1818	CDD:473053
tif471	NP_594602.1	Herpes_BLLF1	<2..290	CDD:282904	28/119 (24%)
		PRK10263	<215..>567	CDD:236669	104/532 (20%)
		tolA_full	470..>574	CDD:274303	32/176 (18%)
		PTZ00121	<501..756	CDD:173412	66/348 (19%)
		eIF_4G1	793..846	CDD:432365	16/74 (22%)
		MIF4G	1009..1244	CDD:397130	98/246 (40%)