Protein Alignment DNF3 and CG33298

DIOPT Version :10

• Sequence 1: NP_013885.1
• Gene: DNF3 / 855197
• SGDID: S000004772
• Length: 1656
• Species: Saccharomyces cerevisiae
• Sequence 2: NP_995666.1
• Gene: CG33298 / 2768929
• FlyBaseID: FBgn0032120
• Length: 1517
• Species: Drosophila melanogaster
• Alignment Length: 1552
• Identity: 410/1552 - (26%)
• Similarity: 649/1552 - (41%)
• Gaps: 431/1552 - (27%)

Yeast   135 NKPYCDNRITSSRYTFYSFLPRQLYAQFSKLANTYFFIVAVLQMIPGWSTTGTYTTIIPLCVFMG 199
            |..:..|:|.:::||..||:|:.|..||.::||.||..:.:|..:|..|..|....:||:...:|
  Fly   247 NGQFVGNKIRTTKYTLLSFIPKNLLEQFHRVANLYFIFIVLLNWVPEISAFGKEVAMIPVLFVLG 311

Yeast   200 ISMTREAWDDFRRHRLDKEENNKPVGVLVKDGNNDAQEVYTLPSSVVSSTAYLTKSAAAENNPPL 264
            ::..::.::|.||...||..||....|.  ||..:
  Fly   312 VTAVKDLFEDRRRRASDKRINNTTCRVY--DGETE------------------------------ 344

Yeast   265 NDDRNSSQGHFLDTHFNNFELLKNKYNVHIHQKKWEKLRVGDFVLLTQDDWVPADLLLLTCDGEN 329
                                    :|.    :.||::|||||.|.|:.::.||||:|||......
  Fly   345 ------------------------RYK----KVKWQELRVGDIVHLSNNETVPADILLLRTSDPQ 381

Yeast   330 SECFVETMALDGETNLKSKQ---PHPELNKLTKAASGLANINAQVTVEDPNIDLYNFEGNLELKN 391
            ..|:::|..||||||||.::   ...|:..:...:..::.:.|    :.|...||.|.|.|
  Fly   382 GVCYIDTCDLDGETNLKRREVVRGFEEMQSIFVPSKFVSRVEA----DAPTTKLYRFHGAL---- 438

Yeast   392 HRNDTIMKYPLGPDNVIYRGSILRNTQNVVGMVIFSGEETKIRMNALKNPRTKAPKLQRKIN--- 453
             .:.|..:.|:..:.::.|.|.|:||..:.|:|:::|.|||..:|. ..||.|..::::::|
  Fly   439 -IHPTGERVPISTECLLLRESRLKNTDYIEGIVVYAGHETKSMLNN-SGPRYKRSQVEQQMNIDV 501

Yeast   454 ---MIIVFMVFVVATISLFSYLGHVLH-KKKYIDQNK------AWYLFQADAGVAPTIMSFIIMY 508
               :||:.::.||..|....:|....| ...|:..||      :.::|          .::|::.
  Fly   502 IWCVIILLILCVVGAIGCRMWLSSFTHFPVPYLPPNKLTANMESMWIF----------WTYIVIL 556

Yeast   509 NTVIPLSLYVTMEIIKVVQSKMMEWDIDMYHAETNTPCESRTATILEELGQVSYIFSDKTGTLTD 573
            ..:||||||||:|:.|::|...:..::|::.||||...|.|...|.|||||:.:||:|||||||:
  Fly   557 QVMIPLSLYVTIELCKILQVFHIHNNVDLFDAETNKQTECRAMNITEELGQIQHIFTDKTGTLTE 621

Yeast   574 NKMIFRKFSLCGSSWLHNVDLGNSE------------------DNF-EDNRDNTNSLRLPPKAHN 619
            ||||||:..:.||.:.|    ..||                  ||. .|....|....|.|.|..
  Fly   622 NKMIFRRCVVNGSDYNH----PPSELEKIYSKPGAPAPPLIPNDNLNSDMAQLTQGTYLTPHAQR 682

Yeast   620 GSSI------------------DVVSIGDQNVLDRLGFSDAPIEKGH------------------ 648
            ....                  |:::......:.::|.|.|.::.|.
  Fly   683 IQEFLVVLAICNTVIVGAAPHRDMMNASGIIEVQQIGNSPANLKHGKQRQKLLASSTSTTTTTTI 747

Yeast   649 ------RPSLDNFPKSRNSIEYKGNSSAIYTGRPSMRSLFG-KDNSH--------LSKQASVISP 698
                  :|.:.:.|..|   ..:...|...|..|....||. ...||        .|.::|..|.
  Fly   748 INGPTTQPQVVSIPADR---YIRLAESRSVTPSPPPNLLFALPAQSHQPTLSPISSSAESSPNSE 809

Yeast   699 SETFSENIKSSFDLIQFIQRYPT----ALFSQKAKFFFLSLALCHSCLPKKTHNESIGEDSIE-- 757
            ||:.|..:|:. .|...|.  ||    |:.:.|    ..|:|   :.|..||..:.:...|.:
  Fly   810 SESPSPPMKNK-SLSNSIS--PTGRAKAVINSK----ITSIA---TFLNAKTQGKRMKLPSSKTG 864

Yeast   758 -----------YQSSSPDELALVTAARDLGYIVLNRNAQILTIKTFPDGFDGEAKLENYEILNYI 811
                       |::.||||||||.||......:|||:...:.:.....|...|     ||||..:
  Fly   865 TIYRTADGRPLYEAESPDELALVNAAYSYDCCLLNRSPNQILVSMPMAGATRE-----YEILKVL 924

Yeast   812 DFNSQRKRMSVLVRMPNQPNQVLLICKGADNVIMERLHDRELAAKKMADICTSTKERKDAEAELV 876
            .|:|.||.||::||.... .:::|..||||:.||..|
  Fly   925 PFDSSRKCMSIVVRQIGS-QEIVLYTKGADSSIMPVL---------------------------- 960

Yeast   877 LQQRKSLERMVDEEAMARTSLRNSLSSVPRASLSLQAVRKSLSMKNSRTRDPEKQIDSIDQFLET 941
                                       ||
  Fly   961 ---------------------------VP------------------------------------ 962

Yeast   942 VKKSDQEIGSVVNKSRKSLHKQQIEKYGPRISIDGTHFPNNNVPIDTRKEGLQHDYDTEILEHIG 1006
                                                  .::|.|     ||:
  Fly   963 --------------------------------------CSHNSP-----EGI------------- 971

Yeast  1007 SDELILNEEYVIERTLQAIDEFSTEGLRTLVYAYKWIDIGQYENWNKRYHQAKTSLTDRKIKVDE 1071
                      :.|:|.|.:|.::.||||.||.|.:.::...|.:|..|:.:.:.||.:|:.::.:
  Fly   972 ----------LREQTQQLLDRYAREGLRILVMAKRTLNSADYTDWWARHQEIEMSLENRERRLRD 1026

Yeast  1072 AGAEIEDGLNLLGVTAIEDKLQDGVSEAIEKIRRAGIKMWMLTGDKRETAINIGYSCMLIKDYST 1136
            :.|::|..|.|||.|.|||:|||||.|.|..:..|||.:|:|||||.||||||.||..|......
  Fly  1027 SFAKLESNLTLLGATGIEDRLQDGVPETIASLLSAGISVWVLTGDKPETAINIAYSAKLFTQQME 1091

Yeast  1137 VVILTTTDENII-SKMNAVSQEVDSGNIAHCV------------VVIDGATMA-MFEGNPTYMSV 1187
            ::.||....:.. :.:|....::::......:            :|:||.|:. :.:.....:..
  Fly  1092 LIRLTARSRDAAETAINFYLTDMENDKTTSTLGYGQSLRKKQRALVVDGKTLTFILDPKSKLILP 1156

Yeast  1188 FVELCTKTDSVICCRASPSQKALMVSNIRNTDPNLVTLAIGDGANDIAMIQSADIGVGIAGKEGL 1252
            |:.|..:..||:|||::|.|||.:|..::. :.||.||||||||||::|||.||:||||:|:||:
  Fly  1157 FLRLSKRCASVLCCRSTPLQKAYLVKVVKE-ELNLRTLAIGDGANDVSMIQMADVGVGISGQEGM 1220

Yeast  1253 QASRVSDYSIGQFRFLLKLLFVHGRYNYIRTSKFMLCTFYKEITFYFTQLIYQRYTMFSGSSLYE 1317
            ||...:|:::.:||:|.:||..||.:.|.|.|:.:|..|||...|.|....||.|..|||..:.:
  Fly  1221 QAVMAADFTLPRFRYLERLLLAHGYWCYDRLSRMILYFFYKNAAFVFLIFWYQLYCGFSGQVMMD 1285

Yeast  1318 PWSLSMFNTLFTSLPVLCIGMFEKDLKPMTLLTVPELYSYGRLSQGFN----WLIFMEWVILATT 1378
            ...|.::|.:|||||.|.||:::|.:....||..|.||..|||...:.    |||.::    |..
  Fly  1286 QMYLMLYNLIFTSLPPLAIGVYDKRVAEDLLLKNPYLYKNGRLGVAYRPHDFWLILLD----ALY 1346

Yeast  1379 NSLIITFLNVVMWGMSSLSDNTMYPLGLINFTAIVALINVKSQFVEMHNRNW--LAFTSVVLSCG 1441
            .||:|.|:.:..:   :.||..::..| ...||.....|:....:|:  |:|  |...|:|||.|
  Fly  1347 QSLVIFFVALCAY---AESDVGIWEFG-TTITASCLFANLVHCAIEI--RSWTVLHVLSIVLSLG 1405

Yeast  1442 GWLVWCCALPILNNTDQIYDVAYGFYNHFGKDITFW----C-----------TSLVLALLPITLD 1491
            .:.::..          :||..  ..|.||...::|    |           .|.|:|:||   .
  Fly  1406 SFYLFAI----------VYDSV--CMNCFGVRSSYWVIFVCFASAVHWLVIMLSTVVAVLP---R 1455

Yeast  1492 IVYKTFKVMIWPSDSDIFAELEQKSDIRKKLELGAYSEMRQGWTWDKDPSTFTRYTD 1548
            ::..|.::.:.|.||       .|..::.|.|......:...|:.....|:..|.||
  Fly  1456 LLLTTVRISLCPDDS-------TKVILQSKRERSRGEALLVTWSRSTSASSIYRITD 1505

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
DNF3	NP_013885.1	P-type_ATPase_APLT_Dnf-like	140..1386	CDD:319770	369/1366 (27%)
CG33298	NP_995666.1	P-type_ATPase_APLT_Dnf-like	252..1355	CDD:319770	370/1367 (27%)