Protein Alignment SHANK3 and shank3a

DIOPT Version :10

• Sequence 1: NP_001358973.1
• Gene: SHANK3 / 85358
• HGNCID: 14294
• Length: 1806
• Species: Homo sapiens
• Sequence 2: XP_073786323.1
• Gene: shank3a / 557701
• ZFINID: ZDB-GENE-060503-369
• Length: 1952
• Species: Danio rerio
• Alignment Length: 2002
• Identity: 1182/2002 - (59%)
• Similarity: 1373/2002 - (68%)
• Gaps: 287/2002 - (14%)

Human    14 PAEPPEPLSPAPAPAPA--------------PPGPLPRSAADGAPAGGKGGPGRRAESPGAPFPG 64
            |..|.||.|..|:.:|:              |..||||..:...      ..|..|.||.|....
Zfish    23 PEVPSEPPSALPSVSPSLSLPLSLPLLIYSRPAQPLPRHLSSST------SRGESARSPHADVCR 81

Human    65 ASGPGPGPGAGMDGPGASAVVVRVGIPDLQQTKCLRLDPAAPVWAAKQRVLCALNHSLQDALNYG 129
            |      |...|:.|..:.:|||:||||||||||::.:..:|:|.:|||:||.||.||:|.||||
Zfish    82 A------PAGPMEEPQGNTLVVRIGIPDLQQTKCMKFNVESPIWLSKQRILCTLNQSLKDVLNYG 140

Human   130 LFQPPSRGRAGKFLDEERLLQEYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKF 194
            ||.|...|:|||||||:|.|:|||....:|:||||||||||||.|..:|:||.:|||||||||||
Zfish   141 LFLPAYNGKAGKFLDEQRTLKEYPLPAVSPVPYLEFRYKRRVYTQAHLDEKQLSKLHTKANLKKF 205

Human   195 MDYVQLHSTDKVARLLDKGLDPNFHDPDSGECPLSLAAQLDNATDLLKVLKNGGAHLDFRTRDGL 259
            |:|||..:.:||::.|:||||||:||||:|||||::|:||:...:|:|||||||||||||||||:
Zfish   206 MEYVQQRNVEKVSKFLEKGLDPNYHDPDTGECPLTMASQLEGCAELIKVLKNGGAHLDFRTRDGI 270

Human   260 TAVHCATRQRNAAALTTLLDLGASPDYKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGITDEN 324
            ||:|.|.|.:|..||.|||||||||||||||||||||||::.|||..|||||||||||:|..|||
Zfish   271 TALHKAVRTKNHTALITLLDLGASPDYKDSRGLTPLYHSSMVGGDPYCCELLLHDHAQVGCMDEN 335

Human   325 GWQEIHQACRFGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQESCARVLLFRGANRDVRNY 389
            ||||||||||.||||||||||||||||.|||||||||||:||||||:||||||||||||::::||
Zfish   336 GWQEIHQACRHGHVQHLEHLLFYGADMSAQNASGNTALHVCALYNQDSCARVLLFRGANKEIKNY 400

Human   390 NSQTAFQVAIIAGNFELAEVIKTHKDSDVVPFRETPSYAKRRRLAGPSGLASPRPLQRSASDINL 454
            |||||||||||||||:|||:|||||.||||||||||||..|||:.|...|.|.|.|.|||||.||
Zfish   401 NSQTAFQVAIIAGNFDLAEIIKTHKASDVVPFRETPSYTNRRRVTGGDTLTSSRSLPRSASDNNL 465

Human   455 K--GEAQPAASPGPSLRSLP---HQLL-----------------------LQRLQEEKDRDRDAD 491
            .  .:|..|.||.|||||||   |..:                       |||:.||
Zfish   466 NNVADASHAHSPVPSLRSLPPLAHSGIDPAADGSLQSTGSSLSSHSRSPSLQRVTEE-------- 522

Human   492 QESNISG----PLAGRAGQSKISPSGPGGPGPAPGPGPAPPAPPAPPPRGPKRKLYSAVPGRKFI 552
             .|.::|    ||.....:.::|   ||.....|.|.|..|:......||||||||||||||.||
Zfish   523 -ASVLAGRRHMPLMHSHSRGRLS---PGTVQRDPSPPPVGPSHTLSGARGPKRKLYSAVPGRTFI 583

Human   553 AVKAHSPQGEGEIPLHRGEAV-------------------------------------------- 573
            .||.:||||||||.|:|||.|
Zfish   584 VVKPYSPQGEGEIQLNRGERVKDYHSNAVEEENVYEEIKLESIKESEEEESPYVEHEIKEEDAHA 648

Human   574 ----KVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQHDTRPETREDRTKRLFRHYTVGSYDSL 634
                ||||||||||||||||||||||||||||||||||:|.|.||||||.|||||||||||||:.
Zfish   649 CVDDKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQYDPRLETREDRNKRLFRHYTVGSYDNF 713

Human   635 TSHSDYVIDDKVAVLQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWRAGL 699
            ||:|||:|::|.|:|||:::|||||||||||||||||||||||||||||||||||||||||||||
Zfish   714 TSYSDYIIEEKNAMLQKKENEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDVEGVAWRAGL 778

Human   700 RTGDFLIEVNGVNVVKVGHKQVVALIRQGGNRLVMKVVSVTRKPE-EDGARRRAPPPPKRAPSTT 763
            |||||||||||||||||||||||:|||||||.|:|||.||||||| |:..|::||||||||||||
Zfish   779 RTGDFLIEVNGVNVVKVGHKQVVSLIRQGGNSLLMKVASVTRKPESEETVRKKAPPPPKRAPSTT 843

Human   764 LTLRSKSMTAELEELASIRRRKGEKLDEMLAAAAEPTLRPDIADADSRAATVKQRPTSRRITPAE 828
            |||||||||||||||||.|||:||:|||:| |:.|..|||.: :||.||||||||||||||||||
Zfish   844 LTLRSKSMTAELEELASARRRRGERLDEIL-ASQETVLRPKV-EADYRAATVKQRPTSRRITPAE 906

Human   829 ISSLFERQGL-------PGPEKLPGSLRKGIPRTKSVG--EDEKLASL-LEGRFPRSTSMQDPVR 883
            ||||||||||       |..||....|.|.:.||||.|  |:::|::| .|.|||||:||.|.:|
Zfish   907 ISSLFERQGLALHGALHPALEKAHMQLPKSMARTKSFGATEEDRLSALAAEHRFPRSSSMTDSLR 971

Human   884 EGRGIPPPPQTAPPPPPAPYYFDSGPPPAFSPPPPPGRAYDTVRSSFKPGLEARLGA----GAAG 944
            :...||||||.||||||:.||.|:||||:|.|||||||.:|..|||||||.|.||.|    .:|.
Zfish   972 DSHSIPPPPQMAPPPPPSLYYLDTGPPPSFCPPPPPGRLHDPSRSSFKPGSEPRLHALPQTMSAE 1036

Human   945 LYEPGAALGPLPYPERQKRARSMIILQDSA---PESGDAPRPPPAATPPERPKRRPRPPGPDSPY 1006
            ||||.....   :.||||:||||||||||:   .|..|.|||.|.:|||||.||:.|.  .|:||
Zfish  1037 LYEPPRHAS---HMERQKKARSMIILQDSSHLPVEPTDIPRPGPVSTPPERIKRKGRI--IDNPY 1096

Human  1007 ANLGAFSASLF--APSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPGGGSFAREPSPTHR--GP 1067
            ||:|.||..:|  .||||||||||||||||||||||||:||:.|      ..|||.||:.|  ..
Zfish  1097 ANVGQFSVGMFTPTPSKPQRRKSPLVKQLQVEDAQERASLALAS------IHAREHSPSGRLAAQ 1155

Human  1068 RPGGLDY-----------GAGDGPGLAFG-GPGPA--------KDRRLEERRRSTVFLSVGAIEG 1112
            .....|:           ......||..| ||..|        ::|||||||:||||||||.:||
Zfish  1156 HTSRSDFFHQQQQLLHERSQAHAEGLLQGKGPFAAAIADAVKDRERRLEERRKSTVFLSVGTMEG 1220

Human  1113 -SAPGADLPSLQPSRSIDERLLGTGPTAGRDL-LLPSPVSALKPLVSGPSLGPSGSTFIHPLTGK 1175
             |||..:.|||.||||:|||:|      .|:| .||.|..||:|       .|.|:|||||||||
Zfish  1221 SSAPPPEAPSLTPSRSVDERML------TRELGQLPPPALALRP-------SPGGTTFIHPLTGK 1272

Human  1176 PLDPSSPLALALAARERALA--SQAPSRSPTPVHSPDADRPGPLFVDVQARDPER----GSLASP 1234
            ||||:||||||||||||||.  ||:||.||.| .:......|.|:::.|.::.||    |..|||
Zfish  1273 PLDPNSPLALALAARERALTSQSQSPSSSPEP-RTKHETAGGVLYMETQTKEAERSEGEGGFASP 1336

Human  1235 AFSPRSP------------AW-IPVPARREAE-KVPREERKSPEDKKSMILSVLDTSLQRPAGLI 1285
            .|||.:.            .| .|:..|:|.| :..|:|.:..|||||||:|::|||.|:.||||
Zfish  1337 PFSPATKRIYETSVPTSKIQWGSPISTRKELETRTERKEERKIEDKKSMIISIMDTSQQKTAGLI 1401

Human  1286 VVHATSNGQE---------PSRLG-GAEEERPGTPEL----APAPMQSAAVAEPLP--SPRAQPP 1334
            :||||||||.         ||..| .:|..:|...|:    :|.| ::.:::.|.|  :|.:|||
Zfish  1402 MVHATSNGQAVGPSPERSVPSPKGILSESPKPMLAEIKRAKSPGP-EAPSISTPPPTAAPTSQPP 1465

Human  1335 GGTPADAGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGVLLATPLA 1399
            ....:|....||||||:.| .:.|.||||.||||||||||||.:||:||||:||:||:|..||..
Zfish  1466 PSPSSDKTLAQGSSEEDAE-PYTVTLPPAMLSSSDEETREELRKIGVVPPPDEFSNGLLAKTPET 1529

Human  1400 GPGPSPT---TVPSPASGKPSSEPPPA------------------------------PESAADSG 1431
            ...|:||   |.||.....|.:.||||                              ||||||||
Zfish  1530 AVPPAPTLTQTTPSTLPSAPPAPPPPAPSAAVAAAAQVPASSGLQAASGKPSESHLGPESAADSG 1594

Human  1432 VEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFADGHTFLLEKPPVPPKPKLKSP 1496
            |||.|||||||.|||||||||||||||||||||||.|||:|:.|||.||:|:||||||||||||.
Zfish  1595 VEEVDTRSSSDHHLETTSTISTVSSMSTLSSESGEPTDTYTTHADGQTFILDKPPVPPKPKLKSQ 1659

Human  1497 LGKGPVTFRDPLLKQSSDSELMAQQHHAASAGLASAAGPARPRYLFQRRSKLWGDPVESRGLPGP 1561
            |.|||||||||||||||||||::|||.||.|..|.|:||||||||||||||||||.||.|.|.|.
Zfish  1660 LSKGPVTFRDPLLKQSSDSELLSQQHAAALAAAAGASGPARPRYLFQRRSKLWGDTVEPRPLSGA 1724

Human  1562 EDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAARLFSSLGELSSISAQRS 1626
            |:.||||||||||||||||||||||||||   ||:.|||.:|||:...|||||||||.:|| ||
Zfish  1725 EEGKPTVISELSSRLQQLNKDTRSLGEEP---LGASLDPGRKSPVVGPRLFSSLGELHTIS-QR- 1784

Human  1627 PGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLG--AGAGGAGRPFGLTPPTILKSSSL 1689
                |.|.:|::||..||||.||.|||..|   :|.:.||  .|.|.|..|  :|||||||||||
Zfish  1785 ----GYGTTYTIRPGSRYPVTRRTPSPGSP---DRSDPLGPIRGFGLATSP--ITPPTILKSSSL 1840

Human  1690 SIPHEPKEVRFVVRSVSARSRSPSPSPLPSPASGPGPGAPGP---RRPFQQKPLQLWSKFDVGDW 1751
            |:||||||||||:||.|||||||||:        |.||...|   .|||.||||.||:|:|||||
Zfish  1841 SLPHEPKEVRFVMRSSSARSRSPSPA--------PSPGMTSPLLTLRPFHQKPLHLWNKYDVGDW 1897

Human  1752 LESIHLGEHRDRFEDHEIEGAHLPALTKDDFVELGVTRVGHRMNIERALRQL 1803
            |.||:|.||||||::|||||:|||||||:|:.|||||||||||||||||:||
Zfish  1898 LASINLAEHRDRFQEHEIEGSHLPALTKEDYAELGVTRVGHRMNIERALKQL 1949

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SHANK3	NP_001358973.1	Intramolecular interaction with the ANK repeats. /evidence=ECO:0000250	76..150		43/73 (59%)
		FERM_F0_SHANK3	83..169	CDD:340697	53/85 (62%)
		ANKYR	155..408	CDD:440430	193/252 (77%)
		ANK repeat	223..255	CDD:293786	21/31 (68%)
		ANK 1	223..253		19/29 (66%)
		ANK repeat	257..288	CDD:293786	21/30 (70%)
		ANK 2	257..286		19/28 (68%)
		ANK repeat	290..355	CDD:293786	54/64 (84%)
		ANK 3	290..320		23/29 (79%)
		ANK 4	324..353		27/28 (96%)
		ANK repeat	357..388	CDD:293786	25/30 (83%)
		ANK 5	357..386		25/28 (89%)
		ANK 6	390..420		26/29 (90%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	407..467		38/61 (62%)
		SH3_Shank3	549..600	CDD:212917	43/98 (44%)
		PDZ_SHANK1_3-like	639..740	CDD:467228	88/100 (88%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	665..689		23/23 (100%)
		Required for interaction with ABI1. /evidence=ECO:0000250	753..760		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	760..853		70/99 (71%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	871..1021		91/158 (58%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1115..1460		195/414 (47%)
		PRK12323	<1208..>1412	CDD:481241	101/240 (42%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1475..1525		41/49 (84%)
		SH3-binding. /evidence=ECO:0000255	1485..1491		5/5 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1546..1584		30/37 (81%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1627..1664		16/36 (44%)
		SAM_Shank1,2,3	1739..1804	CDD:188905	52/65 (80%)
shank3a	XP_073786323.1	None