DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ap2a2 and AP-2alpha

DIOPT Version :9

Sequence 1:	NP_112270.2	Gene:	Ap2a2 / 81637	RGDID:	71015	Length:	939	Species:	Rattus norvegicus
Sequence 2:	NP_995607.1	Gene:	AP-2alpha / 33211	FlyBaseID:	FBgn0264855	Length:	952	Species:	Drosophila melanogaster

Alignment Length:	941	Identity:	647/941 - (68%)
Similarity:	759/941 - (80%)	Gaps:	46/941 - (4%)

- Green bases have known domain annotations that are detailed below.

  Rat    23 CKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNR 87
            |||||||:||||||||||||||||||.||||.||||||||||||||||||||||||||||||||:
  Fly    34 CKSKEAEVKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNK 98

  Rat    88 YTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIP 152
            |:||||||||||||||:||:|||||..:|||||.||||..:.|||.||||:|||:|||:|:.|||
  Fly    99 YSEKQIGYLFISVLVNTNSDLIRLIIQSIKNDLQSRNPVHVNLALQCIANIGSRDMAESFSNEIP 163

  Rat   153 KILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQK 217
            |:||:|||||.||||||||||||:|:|||::|.|:||||::|||||||:||||||||||..|.::
  Fly   164 KLLVSGDTMDVVKQSAALCLLRLFRSSPDIIPGGEWTSRIIHLLNDQHMGVVTAATSLIDALVKR 228

  Rat   218 NPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCY-PPPEDPAVRGRLT 281
            ||:|:|..|:|||||||||||::.|||||||||||||||||||||||||.| |..|:..||.||.
  Fly   229 NPDEYKGCVNLAVSRLSRIVTASYTDLQDYTYYFVPAPWLSVKLLRLLQNYNPVTEEAGVRARLN 293

  Rat   282 ECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLR 346
            |.|||||||||||||||||||||||||||||||:||||.||||||||||||||||||.:||||||
  Fly   294 ETLETILNKAQEPPKSKKVQHSNAKNAVLFEAINLIIHSDSEPNLLVRACNQLGQFLSNRETNLR 358

  Rat   347 YLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLS 411
            ||||||||.||:||||||.||.|.|.||.::|.|:||||||.|||||||||||.||::||.|||:
  Fly   359 YLALESMCHLATSEFSHEEVKKHQEVVILSMKMEKDVSVRQMAVDLLYAMCDRGNAEEIVQEMLN 423

  Rat   412 YLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQ 476
            ||||||||||||:||||||||||||.|||||||.|||||||||||||||||||||||||||::||
  Fly   424 YLETADYSIREEMVLKVAILAEKYATDYTWYVDVILNLIRIAGDYVSEEVWYRVIQIVINREEVQ 488

  Rat   477 GYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALL 541
            |||||||||||||||||||:||||||||||||||||||.||:||:||.|||||:||||..|||||
  Fly   489 GYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDSRSAPLVQFKLLHSKYHLCSPMTRALL 553

  Rat   542 LSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFP 606
            |||||||:|||||::..||||.|..|.|::||.||||||.|||:||.|||||:|||||||||.||
  Fly   554 LSTYIKFINLFPEIRTNIQDVFRQHSNLRSADAELQQRASEYLQLSIVASTDVLATVLEEMPSFP 618

  Rat   607 ERESSILAKLKKKKGPSTV--TDLEETKRERSIDVNGGPEPVPASTSAA-------------STP 656
            ||||||||.||||| |..|  .::.|:|         .|.|:   ||||             :..
  Fly   619 ERESSILAVLKKKK-PGRVPENEIRESK---------SPAPL---TSAAQNNALVNNSHSKLNNS 670

  Rat   657 SPSADLLGLGAVPPAPTGPPPTSGGGLLVDVFSD--------SASAVAPLAPGSEDNFARFVCKN 713
            :.:.||||| :.||:......::....|:||..|        ::|||.        |..:|:.||
  Fly   671 NANTDLLGL-STPPSNNIGSGSNSNSTLIDVLGDMYGSNSNNNSSAVY--------NTKKFLFKN 726

  Rat   714 NGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTV 778
            |||||||::||||:||||||||||:.:||||||.....||.|.|..:.:....||:|.|.|:||:
  Fly   727 NGVLFENEMLQIGVKSEFRQNLGRLGLFYGNKTQVPLTNFNPVLQWSAEDALKLNVQMKVVEPTL 791

  Rat   779 DGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQ 843
            :.|||:||::..|||.|:.:||.:.|.|||.||.|..|:|||:::||||:||||.::.||.|||.
  Fly   792 EAGAQIQQLLTAECIEDYADAPTIEISFRYNGTQQKFSIKLPLSVNKFFEPTEMNAESFFARWKN 856

  Rat   844 LSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLE 908
            ||..||..|.:|||..|:|....:.|::|||..||::|||||.|.|.||||||::.|:|||:|||
  Fly   857 LSGEQQRSQKVFKAAQPLDLPGARNKLMGFGMQLLDQVDPNPDNMVCAGIIHTQSQQVGCLMRLE 921

  Rat   909 PNLQAQMYRLTLRTSKDTVSQRLCELLSEQF 939
            ||.||||:|||:|.||:||::.:|:||::||
  Fly   922 PNKQAQMFRLTVRASKETVTREICDLLTDQF 952

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ap2a2	NP_112270.2	Lipid-binding	5..80		53/56 (95%)
		Phosphatidylinositol 3,4,5-trisphosphate binding. /evidence=ECO:0000305\|PubMed:12086608, ECO:0000305\|PubMed:25061211, ECO:0007744\|PDB:2VGL, ECO:0007744\|PDB:4UQI	11..12
		Adaptin_N	29..591	CDD:396262	457/562 (81%)
		Phosphatidylinositol 3,4,5-trisphosphate binding. /evidence=ECO:0000305\|PubMed:12086608, ECO:0000305\|PubMed:25061211, ECO:0007744\|PDB:2VGL, ECO:0007744\|PDB:4UQI	57..61		3/3 (100%)
		HEAT repeat	332..355	CDD:293787	20/22 (91%)
		HEAT repeat	369..398	CDD:293787	20/28 (71%)
		HEAT repeat	408..434	CDD:293787	23/25 (92%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	616..682		22/80 (28%)
		Alpha_adaptinC2	717..820	CDD:197886	55/102 (54%)
		Alpha_adaptin_C	826..934	CDD:367023	62/107 (58%)
AP-2alpha	NP_995607.1	Adaptin_N	40..598	CDD:279882	454/557 (82%)
		Alpha_adaptinC2	730..839	CDD:197886	58/108 (54%)
		Alpha_adaptin_C	839..947	CDD:280460	62/107 (58%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166349232
Domainoid	1	1.000	898	1.000	Domainoid score	I46
eggNOG	1	0.900	-	-		E1_COG4354
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H5335
Inparanoid	1	1.050	1290	1.000	Inparanoid score	I148
OMA	1	1.010	-	-		QHG53727
OrthoDB	1	1.010	-	-		D751651at2759
OrthoFinder	1	1.000	-	-		FOG0002465
OrthoInspector	1	1.000	-	-		otm46233
orthoMCL	1	0.900	-	-		OOG6_101447
Panther	1	1.100	-	-	LDO	PTHR22780
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1882
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	16	15.770