Protein Alignment CALR and Cnx99A

DIOPT Version :10

• Sequence 1: NP_004334.1
• Gene: CALR / 811
• HGNCID: 1455
• Length: 417
• Species: Homo sapiens
• Sequence 2: NP_477157.1
• Gene: Cnx99A / 44643
• FlyBaseID: FBgn0015622
• Length: 605
• Species: Drosophila melanogaster
• Alignment Length: 543
• Identity: 158/543 - (29%)
• Similarity: 233/543 - (42%)
• Gaps: 151/543 - (27%)

Human    14 LAVAEPAV-----YFKEQFLDGDGWTSRWIESK-HKSDFGKFVLSSGKFYGDEE-------KDKG 65
            ||...|.:     :|.:.|.|.:....||:.|: .|.|..:.:......:..|.       .|.|
  Fly    60 LAYESPVIDAKKFHFADHFDDVEESRKRWVLSQAKKDDIAEEISKYDGIWNWESPQRIVWANDLG 124

Human    66 LQTSQDARFYALSASF-EPFSNKG-QTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDM---HGD 125
            |.....|:..|::|.. :||..|. :.||||:.|..::..:|||.|:||.....|...:   :..
  Fly   125 LVLKSKAKHAAIAAPLRKPFEFKSDKPLVVQYEVTLQEGQECGGSYLKLLSAGKDTEQLKAFNDK 189

Human   126 SEYNIMFGPDICGPGTKKVHVIFNY--------------KGKNVLINKDIRCKD---DEFTHLYT 173
            :.|.||||||.|| ...|:|.||.:              |.||       |.::   |:..|||.
  Fly   190 TPYTIMFGPDKCG-NDVKMHFIFRHVNPINGTITEKHCNKPKN-------RLEEPFKDKLPHLYQ 246

Human   174 LIVRPDNTYEVKIDNSQVESGSLEDDW--DFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDW 236
            |:|||||::|:::|:..:..|||..|:  ...||.:|.||:..|||.||||.||.|||..|||||
  Fly   247 LVVRPDNSFEIRVDHKIINEGSLLTDFKPPVNPPAEIDDPNDHKPESWDEREKIPDPTAHKPEDW 311

Human   237 ---------------------DKPEHIPDPDAKKPEDWDEEMDGEWE------------------ 262
                                 |:|:.|.||.|.||||||.|:|||||
  Fly   312 DEDAPPQLPDTDAVMPNGWLEDEPDMIFDPTATKPEDWDAEIDGEWEAPLVDNPVCEKAPGCGKW 376

Human   263 -PPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTI 326
             .|:|.||.|||:|:...|:||:|:|.|...:|.||::..|...:.......:||:||.:.|..:
  Fly   377 KAPLIPNPNYKGKWRAPMIENPNYQGKWAPRKIPNPDFFEDLKPFQMTPISAVGLELWSMSSDIL 441

Human   327 FDNFLITNDEAYAEEFGNET-----------------------------WGV------------- 349
            |||.:||:|...|.:|...:                             ||:
  Fly   442 FDNLIITDDVEVARDFAANSFDIKRRYIDRESDSFVNKVVELAKANPSIWGIGLVAIVALVALTI 506

Human   350 --------------TKAA--EKQMKDKQDEEQRLKEEEEDKKRKEEEEAE------DKEDDEDKD 392
                          .|||  .|:..|.|.:::...|||.|::...:...|      ..:.::..|
  Fly   507 YCRFGTAKSQDSAAKKAAAEAKKSDDPQPDDEPEAEEESDERAAGDTSKESTPLSASPKKNQKSD 571

Human   393 EDEEDEEDKEEDEEEDVPGQAKD 415
            .|:.:||.|..:..|  |.|.::
  Fly   572 LDDNEEESKAAESRE--PAQTEE 592

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CALR	NP_004334.1	N-domain	18..197		62/213 (29%)
		Calreticulin	23..332	CDD:459737	129/380 (34%)
		4 X approximate repeats	191..255		37/86 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	193..278		52/126 (41%)
		P-domain	198..308		59/151 (39%)
		Interaction with PPIB. /evidence=ECO:0000250	237..270		21/51 (41%)
		3 X approximate repeats	259..297		19/56 (34%)
		C-domain	309..417		34/171 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	350..417		19/74 (26%)
		Prevents secretion from ER	414..417		0/2 (0%)
Cnx99A	NP_477157.1	Calreticulin	74..447	CDD:459737	129/380 (34%)