Protein Alignment CACNA1S and Ca-alpha1D

DIOPT Version :10

• Sequence 1: NP_000060.2
• Gene: CACNA1S / 779
• HGNCID: 1397
• Length: 1873
• Species: Homo sapiens
• Sequence 2: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Alignment Length: 1971
• Identity: 1026/1971 - (52%)
• Similarity: 1290/1971 - (65%)
• Gaps: 233/1971 - (11%)

Human    12 RKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPE 76
            |:::..||.|:   ||.||||||:::||||..||.||||||||.:|||||||||:|||||.|.|.
  Fly   732 RQQRRGKPQPD---RPQRALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPG 793

Human    77 DDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQ 141
            .|:|..|..|||:||.||::|:.|..|||:||||:.|..||||:|||:||||||.:|..:..|.|
  Fly   794 SDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQ 858

Human   142 VNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFM 206
            :           .|.| .||||||||||||||||||||||||||||||.|||:|||||||||||:
  Fly   859 L-----------MKDA-FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFV 911

Human   207 VIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHG 271
            :|||||||||||.||:||.|    .|.:.....|...||   |.|.:|. .|.:|.|||.|||.|
  Fly   912 IIIYAIIGLELFSGKLHKAC----RDEITGEYEENIRPC---GVGYQCP-PGYKCYGGWDGPNDG 968

Human   272 ITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGE 336
            ||:|||||.:||||:||:|:||||||||.:.||:|::|.|:||:::::||:||::||:|||||||
  Fly   969 ITNFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGE 1033

Human   337 FTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEI 401
            |:|||.|||:||.|||||||||::||||||:.||||.|  |:|....|.|..|..|.....:...
  Fly  1034 FSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQAE--DIEPDAVGGLISDGKGKQPNEMDST 1096

Human   402 AGLNKIIQFIR----HWRQW-------NRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHN 455
            ..|.:.:..::    .||:.       ||..|..|...|||:.||||:|::|.|||..:|:||:.
  Fly  1097 ENLGEEMPEVQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYG 1161

Human   456 QPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMT 520
            |..||...|:..|...:.|||.|||:|||.||.:.||:|:|||||||||...|.|.||..:|.|.
  Fly  1162 QLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNTGMMP 1226

Human   521 PLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRY 585
            |||:|||||:||||:||:||||.|||||||||||||:||||||||||||||||||||||:|||::
  Fly  1227 PLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF 1291

Human   586 DFEDTEVR-RSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGN 649
            :|:..|.: |.|||.|.|||::|||::|||||.::||.||.||||.|..|.|.||||||||:|||
  Fly  1292 NFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGN 1356

Human   650 YILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGI 714
            ||||||||||||||||:|:||:..:|   ||:..        |:|.::||.     ...|..:|:
  Fly  1357 YILLNVFLAIAVDNLADADSLSEVEK---EEEPH--------DESAQKKSH-----SPTPTIDGM 1405

Human   715 PTTAKLKIDEFESNV-------NEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVP 772
            .....:.||..:..:       :|........:...::||.:.|..::.|||.::|:....|.:|
  Fly  1406 DDHLSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVNTATKILP 1470

Human   773 IPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDI 837
            ||..:|||:||.||:.||.||.:.|.:.|.|.||..|:.|||.||||:|:||:...|::|..||.
  Fly  1471 IPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFDY 1535

Human   838 GFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVL 902
            .||:|||:|::||:.:||..||.|:|||:.||:||||||.|||||:...|:||||||||||||||
  Fly  1536 FFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSSNAISVVKILRVLRVL 1600

Human   903 RPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFA------------C----------- 944
            ||||||||||||||||||:.||:.|||||||||.|||||||            |
  Fly  1601 RPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNI 1665

Human   945 -------------IGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFH 996
                         |||||||||||:|||.||||::||.|.|.||.|||..:..||.|||.::.||
  Fly  1666 MLVTYLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFH 1730

Human   997 FDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFV 1061
            ||:|...|::|||||||||||.|||.:||||.|:.|||:|.|..:|.::|||||:|||||:||||
  Fly  1731 FDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFV 1795

Human  1062 GFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYFEYLMFA 1126
            ||||||||.:||.|||||:||||||.|:::||||:|:|.||||:..||:||:.||||.|||.:|.
  Fly  1796 GFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTIFI 1860

Human  1127 LIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVI 1191
            |||:||:.|.|:.|||......:.|.||:.||.:|.||.:.||.||:.:.||||.||||||:||:
  Fly  1861 LIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVL 1925

Human  1192 GSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESAR-ISSAFFRLFRVMRLIKLLSRAEGVRT 1255
            ||.||::.|||.:...|......:..||.:......:. ||..||||||||||:||||:.||:||
  Fly  1926 GSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIRT 1990

Human  1256 LLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCA 1320
            ||||||||||||||||||||:|||||||:|||:||||||..|..|..|||||||.||||:|||.|
  Fly  1991 LLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSA 2055

Human  1321 TGEAWQEILLACSY--GKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDN 1383
            ||||||||:::||.  ...||..|| .|||  .||::.||.||||||:||:||:|||||||||||
  Fly  2056 TGEAWQEIMMSCSAQPDVKCDMNSD-TPGE--PCGSSIAYPYFISFYVLCSFLIINLFVAVIMDN 2117

Human  1384 FDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRL 1448
            |||||||||||||||||||..:|:||||:|||||||||||||||:|.|||||||.||||:|||||
  Fly  2118 FDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRL 2182

Human  1449 VGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPI 1513
            |.|||||||||||.|||||||:|||:|.|||:||.:.||.||||.||:|||||:.||||||:||.
  Fly  2183 VSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQVVPPP 2247

Human  1514 G-DDEVTVGKFYATFLIQEHFRKFMKR--QEEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVS 1575
            | ||||||||||||:|||::||:|.||  ||...|: |..:.|.:||||||: .|.:|.:.|.:|
  Fly  2248 GNDDEVTVGKFYATYLIQDYFRRFKKRKEQEGKEGH-PDSNTVTLQAGLRTL-HEVSPALKRAIS 2310

Human  1576 GDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEEMES 1640
            |:|   :||::.      .|.:.||...|||.|.:.:.|..                       :
  Fly  2311 GNL---DELDQE------PEPMHRRHHTLFGSVWSSIRRHG-----------------------N 2343

Human  1641 PVFLEDFPQDPRTNPLARANTNNA------------------------------------NANVA 1669
            ..|        |.:..|.|:.:|.                                    |.:||
  Fly  2344 GTF--------RRSAKATASQSNGALAIGGSASAALGVGGSSLVLGSSDPAGGDYLYDTLNRSVA 2400

Human  1670 YGNSNHSNSHVFSSVHYEREFPEETETPATRGRALGQPCRVLGP--HSKPCVEMLKGLLTQRAMP 1732
            .|.:|.:.:.:.:.:....:..:|.:     |...|...|..|.  ..:|..:...|..|    .
  Fly  2401 DGVNNITRNIMQARLAAAGKLQDELQ-----GAGSGGELRTFGESISMRPLAKNGGGAAT----V 2456

Human  1733 RGQAPP-----------------------APCQCPRVESSMP--EDRKSSTPGSLHEETPHSRST 1772
            .|..||                       ||      ..::|  |....|||.|     |:.:..
  Fly  2457 AGTLPPEANAINYDNRNRGILLHPYNNVYAP------NGALPGHERMIQSTPAS-----PYDQRR 2510

Human  1773 RENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALADACQMEPEEVEIMATELLKGRE 1837
            ...:|..:..|.: ||...|...|||.. .|:.|:....:.:.:|..|.|||:.:.|.::|....
  Fly  2511 LPTSSDMNGLAES-LIGGVLAAEGLGKY-CDSEFVGTAAREMREALDMTPEEMNLAAHQILSNEH 2573

Human  1838 APEGMASSLGCLNLGSSLGSL 1858
            :...:.||.|.: .|.|.|.|
  Fly  2574 SLSLIGSSNGSI-FGGSAGGL 2593

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CACNA1S	NP_000060.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..23		4/10 (40%)
		I. /evidence=ECO:0000305	38..337		185/298 (62%)
		Ion_trans	50..345	CDD:459842	182/294 (62%)
		Selectivity filter of repeat I. /evidence=ECO:0000250|UniProtKB:P07293	290..293		1/2 (50%)
		Binding to the beta subunit. /evidence=ECO:0000250|UniProtKB:Q02789	357..374		12/16 (75%)
		II. /evidence=ECO:0000305	418..664		162/246 (66%)
		Ion_trans	431..672	CDD:459842	162/241 (67%)
		Selectivity filter of repeat II. /evidence=ECO:0000250|UniProtKB:P07293	612..615		2/2 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	675..717		9/41 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	731..757		4/25 (16%)
		Interaction with STAC, STAC2 and STAC3 (via SH3 domains). /evidence=ECO:0000269|PubMed:29078335	747..760		4/12 (33%)
		III. /evidence=ECO:0000305	786..1068		188/317 (59%)
		Ion_trans	798..1075	CDD:459842	187/312 (60%)
		Dihydropyridine binding. /evidence=ECO:0000250|UniProtKB:P07293	988..1077		59/88 (67%)
		Selectivity filter of repeat III. /evidence=ECO:0000250|UniProtKB:P07293	1012..1015		2/2 (100%)
		IV. /evidence=ECO:0000305	1105..1384		170/281 (60%)
		Ion_trans	1117..1392	CDD:459842	172/277 (62%)
		Selectivity filter of repeat IV. /evidence=ECO:0000250|UniProtKB:P07293	1321..1324		2/2 (100%)
		Dihydropyridine binding. /evidence=ECO:0000250|UniProtKB:P07293	1337..1403		50/65 (77%)
		Phenylalkylamine binding. /evidence=ECO:0000250|UniProtKB:P07293	1349..1391		31/41 (76%)
		GPHH	1401..1454	CDD:465306	42/52 (81%)
		Ca_chan_IQ	1464..1536	CDD:462591	54/72 (75%)
		Interaction with calmodulin. /evidence=ECO:0000269|PubMed:19473981	1522..1542		14/21 (67%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1731..1780		13/73 (18%)
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	182/294 (62%)
		Ion_trans	1137..1379	CDD:459842	162/241 (67%)
		Ion_trans	1496..1809	CDD:459842	187/312 (60%)
		Ion_trans	1851..2126	CDD:459842	172/277 (62%)
		GPHH	2135..2188	CDD:465306	42/52 (81%)
		Ca_chan_IQ	2198..2273	CDD:462591	55/74 (74%)