Protein Alignment CACNA1A and para

DIOPT Version :10

• Sequence 1: NP_075461.2
• Gene: CACNA1A / 773
• HGNCID: 1388
• Length: 2512
• Species: Homo sapiens
• Sequence 2: NP_001259619.1
• Gene: para / 32619
• FlyBaseID: FBgn0285944
• Length: 2145
• Species: Drosophila melanogaster
• Alignment Length: 2442
• Identity: 538/2442 - (22%)
• Similarity: 910/2442 - (37%)
• Gaps: 834/2442 - (34%)

Human    64 PIPVR---------------------QNCLTV-----NRSLFLFSED---------NVVRKYAKK 93
            |||||                     .|.||.     .:.:|.||..         |.:|:.|..
  Fly    84 PIPVRLQGSFPPELASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIY 148

Human    94 ITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFA 158
            |...|.|...|:.||:.|||::.:          |.:..::.||..|.||:.||:.:|::|.||.
  Fly   149 ILVHPLFSLFIITTILVNCILMIM----------PTTPTVESTEVIFTGIYTFESAVKVMARGFI 203

Human   159 FHKGSYLRNGWNVMDFVVVLTGILATVGTEF-DLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKA 222
            ....:|||:.||.:||||:....: |:|.:. :|..||..||||.||.|:.:|.|:.::.:::::
  Fly   204 LCPFTYLRDAWNWLDFVVIALAYV-TMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIES 267

Human   223 MIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCF-------------EEGTD-----------D 263
            :..|..:.:|..|::.:||::||:.|||.....|.             :|..|           :
  Fly   268 VKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDENWDYHNRNSSNWYSE 332

Human   264 IQGESPAPCGTEEPARTCPNGTKC-QPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNS 327
            .:|.|...||....|..|.:...| |.:...||.|.|.||:..:|.|:.|:.:|.:.|.||....
  Fly   333 DEGISFPLCGNISGAGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLV 397

Human   328 NDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEF------AKERERVEN------------RR 374
            ..|:| .|:.|:||.:|.:|||:::||:|.:::..:      |:|.|..|.            :.
  Fly   398 LRAAG-PWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEAIREAEEAAAAKA 461

Human   375 AFLKLRRQQQIERELNGYMEWISKAEEVIL-----------------------AEDETDGE---- 412
            |.|:.|...|.:...:.     :.|||..|                       ..|:.:.|
  Fly   462 AKLEERANAQAQAAADA-----AAAEEAALHPEMAKSPTYSCISYELFVGGEKGNDDNNKEKMSI 521

Human   413 -------------QRHPF-DGALRRTTIKK-SKTDLLNP-------------------------- 436
                         ||.|. ..|.:.|.::| |.|.|..|
  Fly   522 RSVEVESESVSVIQRQPAPTTAHQATKVRKVSTTSLSLPGSPFNIRRGSRSSHKYTIRNGRGRFG 586

Human   437 --------------EEAEDQL--ADIASVGSPFARAS---------------------------- 457
                          ::|:..|  ||.::..:|.:..:
  Fly   587 IPGSDRKPLVLSTYQDAQQHLPYADDSNAVTPMSEENGAIIVPVYYGNLGSRHSSYTSHQSRISY 651

Human   458 ------------------IKSAKLEN--------------STFF---HKKERR------------ 475
                              .|.:||.|              :|..   ||.:.|
  Fly   652 TSHGDLLGGMAVMGVSTMTKESKLRNRNTRNQSVGATNGGTTCLDTNHKLDHRDYEIGLECTDEA 716

Human   476 --MRFY---IRRMVKTQ-------------------------------AFY-------------- 490
              ::.:   ....|:||                               .:|
  Fly   717 GKIKHHDNPFIEPVQTQTVVDMKDVMVLNDIIEQAAGRHSRASDRGVSVYYFPTEDDDEDGPTFK 781

Human   491 ----------------------------WTVLSL------------VALNTLCVAIVHYNQPEWL 515
                                        |..|.:            :.:||:.:|:.|::..:.:
  Fly   782 DKALEVILKGIDVFCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEM 846

Human   516 SDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGIS 580
            ...|....:.|...|..|..:|:..:..:.||...:|.||..::..|:.|:....::     |:|
  Fly   847 ERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQ-----GLS 906

Human   581 VLRALRLLRIFKVTKYWASLRNLVVSLL-NSMKSIISLLFLLFLFIVVFALLGMQLFGGQFN--- 641
            |||:.||||:||:.|.|.:| ||::|:: .:|.::.:|.|:|.:.|.:||::||||||..::
  Fly   907 VLRSFRLLRVFKLAKSWPTL-NLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHK 970

Human   642 --FDEG-TPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNY 703
              |.:| .|..||..|..:.|.||::|.|| |.|.|:|.:     ..|.:....:|:...:.||.
  Fly   971 DRFPDGDLPRWNFTDFMHSFMIVFRVLCGE-WIESMWDCM-----YVGDVSCIPFFLATVVIGNL 1029

Human   704 TLLNVFLAIAVDNLANAQELTKVEADEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQ 768
            .:||:|||:.:.|..:: .|:...||..              ..::||.
  Fly  1030 VVLNLFLALLLSNFGSS-SLSAPTADND--------------TNKIAEA---------------- 1063

Human   769 KNQKPAKSVWEQRTSEMRKQNLLASREALYNEMDPDERWKAAYTRHLRPDMKTHLDRPLVVDPQE 833
                                         :|.:.   |:|:...|::....|      |:
  Fly  1064 -----------------------------FNRIG---RFKSWVKRNIADCFK------LI----- 1085

Human   834 NRNNNTNKSRAAEPTVDQRLGQQRAEDFLRKQARYHDRARDPSGSAGLDARRPWAGSQEAELSRE 898
             ||..||:      ..||                       |||.
  Fly  1086 -RNKLTNQ------ISDQ-----------------------PSGE-------------------- 1100

Human   899 GPYGRESDHHAREGSLEQPGFWEGEAERGKAGDPHRRHVHRQGGSRESRSGSPRTGADGEHRRHR 963
                       |...:.    |.....:|..     |.:..:.|..|...|.....|||..::
  Fly  1101 -----------RTNQIS----WIWSEGKGVC-----RCISAEHGDNELELGHDEILADGLIKK-- 1143

Human   964 AHRRPGEEGPEDKAERRARHREGSRPARGGEGEGEGPDGGERRRRHRHGAPATYEGDARREDKER 1028
                    |.:::.:...        |.|        ||.|          .|..||
  Fly  1144 --------GIKEQTQLEV--------AIG--------DGME----------FTIHGD-------- 1166

Human  1029 RHRRRKENQGSGVPVSGPNLSTTRPIQQDLGRQDPPLAEDIDNMKNNKLATAESAAPHGSLGHAG 1093
                                                       |||||
  Fly  1167 -------------------------------------------MKNNK----------------- 1171

Human  1094 LPQSPAKMGNSTDPGPMLAIPAMATNPQNAASRRT----PNNP-GNPSNPGPPKTPENSLIVTNP 1153
             |:....:.|:||              .:.||..:    .|.| .:.|:.|..:|.|
  Fly  1172 -PKKSKYLNNATD--------------DDTASINSYGSHKNRPFKDESHKGSAETME-------- 1213

Human  1154 SGTQTNSAKTARKPDHTTVDIPPACPPPLNHTVVQVNKNANPDPLPKKEEEKKEEEEDDRG--ED 1216
             |.:...|                                      .||:...:||.|:.|  |:
  Fly  1214 -GEEKRDA--------------------------------------SKEDLGLDEELDEEGECEE 1239

Human  1217 GPK----------------------PMPPYSSMFILSTTNP---------LRRLCHYILNLRYFE 1250
            ||.                      |...|....||:..:.         ||.....::..:|||
  Fly  1240 GPLDGDIIIHAHDEDILDEYPADCCPDSYYKKFPILAGDDDSPFWQGWGNLRLKTFQLIENKYFE 1304

Human  1251 MCILMVIAMSSIALAAEDPVQPNAP-RNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRD 1314
            ..::.:|.|||:|||.||...|..| ..::|.|.|.:||.:|..||:||.:.||..:    ||.:
  Fly  1305 TAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKV----YFTN 1365

Human  1315 LWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKN 1379
            .|..|||::|..:|:.|..:....| .|...|::|.||.||||:.:.|:..::.|.:.:|.::.:
  Fly  1366 AWCWLDFVIVMVSLINFVASLVGAG-GIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPS 1429

Human  1380 VFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYE----KNEVKARDREWK 1440
            :||:|:|.::|..|||::.||||.||:|.|        :|..|..|.:|    :|..::.:..|.
  Fly  1430 IFNVLLVCLIFWLIFAIMGVQLFAGKYFKC--------EDMNGTKLSHEIIPNRNACESENYTWV 1486

Human  1441 KYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIF---YVVYFVVF- 1501
            ....::|:|..|.|.||.|:|.:||.|::..::|:...::.|.    .|.:|:   |.|:|::|
  Fly  1487 NSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPI----RETNIYMYLYFVFFIIFG 1547

Human  1502 PFFFVNIFVALIIITFQEQGDK---MMEEYSLEKNER--ACIDFAISAKPLTRHMPQNKQSFQYR 1561
            .||.:|:|:.:||..|.||..|   .:|.:..|..::  ..:....|.||| :.:|:.:...|..
  Fly  1548 SFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPL-KAIPRPRWRPQAI 1611

Human  1562 MWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGIL 1626
            :::.|....|:..||..|.||...:.:..|.||..|...|...|.:|..:||.||:||:.|.. .
  Fly  1612 VFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALR-Y 1675

Human  1627 NYFRDAWNIFDFVTVLGSITDILVTEFGNPNNFINLSFLRLFRAA---RLIKLLRQGYTIRILLW 1688
            :||.:.||:||.|.|:.||..:::::. ....|::.:.||:.|.|   |:::|::....||.||:
  Fly  1676 HYFIEPWNLFDVVVVILSILGLVLSDI-IEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLF 1739

Human  1689 TFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALML 1753
            ....|..||..:|||:.::.||:||.||..|    :.|:::....|.:      ||:||.|:::|
  Fly  1740 ALAMSLPALFNICLLLFLVMFIFAIFGMSFF----MHVKEKSGINDVY------NFKTFGQSMIL 1794

Human  1754 LFRSATGEAWHNIMLSCLSGKPCD--KNSGILTRECGN-EFAYFYFVSFIFLCSFLMLNLFVAVI 1815
            ||:.:|...|..::.:.::.:.||  .|.......||: .....:.:|::.:...:::|:::|||
  Fly  1795 LFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVI 1859

Human  1816 MDNFEYLTRD-SSILGPHHLDEYVRVWAEYDPAAWGRMPYLDMYQMLRHMSPPLGLGKKCPARVA 1879
            ::|:...|.| ...|.....|.|..:|.::||.....:.|..:.:.|..:.|||.:.|  |.:
  Fly  1860 LENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHK--PNK-- 1920

Human  1880 YKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTL 1944
            || ::.||:|:...:.::....|.||.:   |....||...::  ..|:.:  :|..|:...
  Fly  1921 YK-IISMDIPICRGDLMYCVDILDALTK---DFFARKGNPIEE--TGEIGE--IAARPDTEG--- 1974

Human  1945 DLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQDR-----------TPLMFQRMEP 1998
               ..|..||.....:.|.|.:|...:|:.||:.......|.|.           .|...:..:.
  Fly  1975 ---YEPVSSTLWRQREEYCARLIQHAWRKHKARGEGGGSFEPDTDHGDGGDPDAGDPAPDEATDG 2036

Human  1999 PSP---------TQEG----------GPGQNAL-------------PSTQLDPGGALMAHESG-L 2030
            .:|         |.||          .||::|.             .:|...||......::. |
  Fly  2037 DAPAGGDGSVNGTAEGAADADESNVNSPGEDAAAAAAAAAAAAAAGTTTAGSPGAGSAGRQTAVL 2101

Human  2031 KESPSWVTQ---------RAQEMFQKTGTWSPEQGPP 2058
            .||..:||:         |:..:..:|........||
  Fly  2102 VESDGFVTKNGHKVVIHSRSPSITSRTADVXARPRPP 2138

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CACNA1A	NP_075461.2	I	85..363		94/303 (31%)
		Ion_trans	97..371	CDD:459842	93/305 (30%)
		Binding to the beta subunit. /evidence=ECO:0000250|UniProtKB:P27884	383..400		1/16 (6%)
		II	473..717		78/352 (22%)
		Ion_trans	487..725	CDD:459842	77/329 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	822..1224		58/430 (13%)
		III	1234..1517		97/300 (32%)
		Ion_trans	1247..1478	CDD:459842	82/235 (35%)
		Ion_trans	1568..1827	CDD:459842	79/265 (30%)
		GPHH	1836..1889	CDD:465306	15/52 (29%)
		Ca_chan_IQ	1899..1976	CDD:462591	16/76 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1994..2037		13/75 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2049..2512		2/10 (20%)
		PHA03307	2239..>2509	CDD:223039
		polyglutamine repeat	2319..2331
para	NP_001259619.1	Ion_trans	152..436	CDD:459842	90/295 (31%)
		Na_trans_cytopl	555..740	CDD:463401	19/184 (10%)
		Ion_trans	817..1028	CDD:459842	64/222 (29%)
		Na_trans_assoc	1054..1296	CDD:461936	71/550 (13%)
		Ion_trans	1300..1571	CDD:459842	99/287 (34%)
		Na_channel_gate	1561..1613	CDD:240441	12/52 (23%)
		Ion_trans	1621..1871	CDD:459842	79/261 (30%)
		GPHH	1882..1929	CDD:465306	15/51 (29%)