Protein Alignment VCL and Vinc

DIOPT Version :10

• Sequence 1: NP_054706.1
• Gene: VCL / 7414
• HGNCID: 12665
• Length: 1134
• Species: Homo sapiens
• Sequence 2: NP_476820.1
• Gene: Vinc / 31201
• FlyBaseID: FBgn0004397
• Length: 961
• Species: Drosophila melanogaster
• Alignment Length: 1148
• Identity: 469/1148 - (40%)
• Similarity: 647/1148 - (56%)
• Gaps: 202/1148 - (17%)

Human     1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTT 65
            ||||||:||||||:|||||:|.|||:|||.| ||.|:|||:.||..|.|||:|||:||::|:.::
  Fly     1 MPVFHTKTIESILDPVAQQVSRLVILHEEAE-DGNAMPDLSRPVQVVSAAVANLVKVGRDTINSS 64

Human    66 EDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAE 130
            :|:||::|||.|..:||.|...|.:|:.||:|||||.|||..||:||||||.|||.|||.|||:|
  Fly    65 DDKILRQDMPSALHRVEGASQLLEEASDMLRSDPYSGPARKKLIEGSRGILQGTSSLLLCFDESE 129

Human   131 VRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSM 195
            |||||:.||.:|:||.||||:.|||.||.:.|:|.|.::|:.:.:..|::|||||.|..:||..:
  Fly   130 VRKIIQECKRVLDYLAVAEVINTMEQLVQFLKDLSPCLSKVHREVGAREKELTHQVHSEILVRCL 194

Human   196 NTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWAS 260
            ..||.|.|:||.:||:::.....:.:|.|||.:|||:...:||.|:.|||||||||::|||....
  Fly   195 EQVKTLAPILICSMKVYIHIVEQQGRGAEEAAENRNYLAARMSDELQEIIRVLQLTTYDEDTSEL 259

Human   261 KDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTC 325
            .:...:|:...:|.:|:.||..||.:|.|..|..||:|:||::|.|.::.|.|.
  Fly   260 DNLTVLKKLSNAISNKMEQANEWLSNPYALRGGVGEKALRQVIDNATEISERCL----------- 313

Human   326 KMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDA 390
                            .|.|.|:
  Fly   314 ----------------PQDSYPI------------------------------------------ 320

Human   391 AQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGD 455
                                     ||:|:                |::|:.:.|.:||::|||.
  Fly   321 -------------------------RKLAD----------------EVTAMANTLCELRQEGKGQ 344

Human   456 SPEARALAKQVATALQNLQTKTNRAVANSRPA---KAAVHLEGKIEQAQRWIDNPTVDDRGVGQA 517
            ||:|.:|.:.:...:..|::..::||.....|   :.|..::|::|||.:|:.:|.::|.|:|:.
  Fly   345 SPQAESLVRGIRDRMGELKSLVHQAVLGVDKAGVQQTAHTIQGRLEQAVKWLQHPEINDGGLGER 409

Human   518 AIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKD 582
            ||..:|.||.::|....|..:.::...||.|::|..|.|       |..|.|:..|.||...|.:
  Fly   410 AINLIVEEGRKVAEGCPGHQKAEIQQLCDEVERLKRQAA-------GSGPAAKQAAKQLTQKLYE 467

Human   583 LKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKA 647
            |||.:|.|:...:...|.|.:||:|....|...|...|.||:.|::::.|.:..|.:...|:...
  Fly   468 LKAAIQNALVNRIVQDFMDVSTPLKQFTEAVLQPEGTPGREQNFNQKSNNLQAFSDRASKTSRMV 532

Human   648 AAVGT-ANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTG 711
            ||.|. .||...|.:.:|......||||::||.||.:..||::||.||.:.:|.|:.|.|.:|..
  Fly   533 AAGGACGNKKIAEILLSSAAQVDSLTPQLISAGRIRMNYPGSKAADEHLQNLKQQYADTVLRMRT 597

Human   712 LVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSED 776
            |.|:|.|....:..|||.::.....|:.|:...|||.:|...::|||..||:|||||:|.:||||
  Fly   598 LCDQATDPADFIKTSEEHMQVYAKLCEDAIHARQPQKMVDNTSNIARLINRVLLVAKQEADNSED 662

Human   777 PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEP- 840
            |.|.|.:.||::.|.:::..||.|||.||.||:||....::.:|..|:||.|.:||:|..|.:|
  Fly   663 PVFTERLNAAANRLERSLPAMVGDAKLVATNIADPAAAAAWKNSFQRLLGDVREVRDAIAPPQPP 727

Human   841 ----DFPPPPPDLEQLRLTD---ELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPM 898
                ..|||.|:|..|.|::   |.|||:||||...:.|.||||||..||:
  Fly   728 PLPTSLPPPIPELSALHLSNQNAERAPPRPPLPREGLAPVRPPPPETDDED-------------- 778

Human   899 MMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVN 963
                                                               |.....||.   .|
  Fly   779 ---------------------------------------------------EGVFRTMPH---AN 789

Human   964 QPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGS-GTKRALIQCAKDIAKASD 1027
            ||||.||:.||:|..:||||.|:|||||||||:|||.:|.||...| |:||.||..||.||:||:
  Fly   790 QPILIAARGLHQEVRQWSSKDNEIIAAAKRMAILMARLSELVLSDSRGSKRELIATAKKIAEASE 854

Human  1028 EVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLV 1092
            :|||||||:|:||||:||||||||||||||||.|||||||||||||||... |||:.| ||||||
  Fly   855 DVTRLAKELARQCTDRRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQG-SDEDRE-ATEMLV 917

Human  1093 HNAQNLMQSVKETVREAEAASIKIRTD-AGFTLRWVRKTPWYQ 1134
            .||||||||||||||.||.||||||:| ....|:|||:.||||
  Fly   918 GNAQNLMQSVKETVRAAEGASIKIRSDQTSNRLQWVRRQPWYQ 960

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
VCL	NP_054706.1	N-terminal globular head. /evidence=ECO:0000269|PubMed:20484056	1..835		313/837 (37%)
		Vinculin	3..1134	CDD:460040	465/1144 (41%)
		Talin-interaction. /evidence=ECO:0000250|UniProtKB:P12003	168..208		16/39 (41%)
		3 X 112 AA tandem repeats. /evidence=ECO:0000255	259..589		74/332 (22%)
		Interaction with ACTN4. /evidence=ECO:0000269|PubMed:15988023, ECO:0007744|PDB:1YDI	741..764		9/22 (41%)
		Linker (Pro-rich). /evidence=ECO:0000269|PubMed:20484056	836..878		21/49 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	857..887		17/29 (59%)
		C-terminal tail. /evidence=ECO:0000269|PubMed:20484056	879..1134		130/256 (51%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1003..1046		27/43 (63%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1120..1134		6/13 (46%)
Vinc	NP_476820.1	Vinculin	3..960	CDD:460040	465/1144 (41%)