Protein Alignment VCL and deb-1

DIOPT Version :10

• Sequence 1: NP_054706.1
• Gene: VCL / 7414
• HGNCID: 12665
• Length: 1134
• Species: Homo sapiens
• Sequence 2: NP_001293743.1
• Gene: deb-1 / 177482
• WormBaseID: WBGene00000942
• Length: 1090
• Species: Caenorhabditis elegans
• Alignment Length: 1271
• Identity: 460/1271 - (36%)
• Similarity: 649/1271 - (51%)
• Gaps: 323/1271 - (25%)

Human     1 MPVFHTRTIESILEPVAQQ---------------------------------------------- 19
            ||||||:|||:||||||||
 Worm     1 MPVFHTKTIENILEPVAQQLHYVLAMAEKNATSVDEVRHLGTSMPNLDRFASRSALALSRASSRR 65

Human    20 ----------------------------------ISHLVIMHEEGEVDGKAIPDLTAPVAAVQAA 50
                                              :|.|||:|||.. ||.|:||||.||..|..|
 Worm    66 SIPEYITPDTVRHVVNDDDCPVCQLMMPRGKDGCVSRLVILHEEAN-DGNAMPDLTGPVGMVSRA 129

Human    51 VSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGI 115
            |.||::||.:|...::|:||::|||||..:||.:...|.:::..|:.||||||||..||||:|||
 Worm   130 VGNLIQVGYDTCDHSDDRILQQDMPPALQRVEGSSKLLEESSYSLKHDPYSVPARKKLIDGARGI 194

Human   116 LSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQ 180
            |.|||.|||.|||:|||||||||:...:|:.|:||:|:|.||..:.|::.|.:..:...::.|||
 Worm   195 LQGTSALLLCFDESEVRKIIRVCRKANDYVAVSEVIESMADLQQFVKDISPVLHDVTNDVNLRQQ 259

Human   181 ELTHQEHRVMLVNSMNTVKELLPVLISAMK--IFVTTKNSKNQGIEEALKNRNFTVEKMSAEINE 243
            |||||.||.:|:..|:::|.:.|:||.:||  |.:.|.:.: ||..||:.||||..::|:.|:||
 Worm   260 ELTHQVHREILIRCMDSIKVIAPILICSMKTSIELGTPHPR-QGHAEAIANRNFMSQRMTEEMNE 323

Human   244 IIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGK 308
            ||||||||::|||.|.:.:...|::||::..|.|..|..||.||.|..|..||:|||:|.:.|.:
 Worm   324 IIRVLQLTTYDEDEWDADNVTVMRKALSAAKSLLTAALDWLADPHARSGAVGEKAIRRICEYADR 388

Human   309 VGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARK 373
            :                                                            :||.
 Worm   389 I------------------------------------------------------------SARA 393

Human   374 LEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEI 438
            |                                                  |::...|.||:.||
 Worm   394 L--------------------------------------------------PEDAQSIKRSIFEI 408

Human   439 SALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNR------AVANSR------PAKAAV 491
            ::.|.:|.:||..|:   |:...||.|.|..|::|....|.      |:.|::      ||..|.
 Worm   409 TSFTDELCNLRNNGQ---PDRENLAAQTARRLKDLVGSQNSSGLMGDALQNAQRHGGANPAHTAA 470

Human   492 HLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLA 556
               |::|||.||:|||.:||.|:|..|:|.|.|:..:||:.:....|..||..|..:|:|.||||
 Worm   471 ---GRLEQALRWLDNPGLDDGGLGLQALRLLTADARKLADRLNPQDRNRLLGLCSDIDRLAAQLA 532

Human   557 DLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPN 621
            ||..||.|.||:|..:.:||:::|:||...|:..:|..|.|.|:|.|||:|....|..|.|..||
 Worm   533 DLERRGLGNSPEAHQIRNQLKNALRDLGDFMRRVLTDRVVDDFADITTPLKQFVEAVHADPYDPN 597

Human   622 REEVFDERAANFENHSGKLGATAEKAAAVG-TANKSTVEGIQASVKTARELTPQVVSAARILLRN 685
            ||:.|.:::....:||..:..||...|:.| :.:|.|||.|..:.:...:||||:|:|.|:.|.|
 Worm   598 REQNFVDKSQRLTDHSQSMTTTARLVASCGPSKSKKTVEAILDTAEKVEQLTPQLVNAGRVRLHN 662

Human   686 PGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLV 750
            ||::   :|||.:..|:.|.:.::...||:||||...:.|||.|:::..:.|:.|:.......||
 Worm   663 PGSE---QHFENIHKQYADALHRLRSHVDDAIDTGEFVRASETAMRRYTNHCEGAINGADAHGLV 724

Human   751 AGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQK 815
            ..::.|||..||:|:.|:.|.:|||:|.|...|:.|:|:|...|.|||.:||.:|.|..|....:
 Worm   725 NNSSQIARLGNRVLMTAQNEADNSEEPSFVSRVRNAADQLHNAIPPMVNNAKQIAQNPHDQYAAQ 789

Human   816 SFLDSGYRILGAVAKV-----------------REAFQPQEPDFPPPPPDLEQLRLT-------- 855
            ::..:...:|.:|..|                 :|:.....| :.||||..:.:|..
 Worm   790 NWRGTNDHLLNSVRAVGDAITGVPMSNGRHSSYQESISRASP-YNPPPPSSQVIRSVNASPPTAP 853

Human   856 ----------DELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEAR 910
                      |..|||:||.|....||||||||.|.|||                      :|.|
 Worm   854 IIHNKMIIREDIPAPPRPPPPVELSPPPRPPPPPEYDEE----------------------EETR 896

Human   911 KWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHR 975
            .:..:                       :|:|                 |..:||:||||.:||.
 Worm   897 AFWER-----------------------YPLP-----------------QASHQPMLAAAHNLHN 921

Human   976 EATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQC 1040
            |..:|||:.|||:|||||||:|||.:|:||||..|||:.||.|:|.||.:|:||||||.::|:.|
 Worm   922 ELKQWSSQENDIVAAAKRMAILMARLSQLVRGEGGTKKDLINCSKAIADSSEEVTRLAVQLARLC 986

Human  1041 TDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNI---------SDEESEQATEMLVHNAQ 1096
            ||.::||.||||.||||||:||||:||||||||||..|:         ..||.::|.:.||||||
 Worm   987 TDIKMRTALLQVSERIPTIATQLKVLSTVKATMLGSANVIGPYGQPVEGSEEDDEAMQQLVHNAQ 1051

Human  1097 NLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPW 1132
            |||||||:.||.||||||||||::|..|||:||..|
 Worm  1052 NLMQSVKDVVRAAEAASIKIRTNSGLRLRWLRKPMW 1087

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
VCL	NP_054706.1	N-terminal globular head. /evidence=ECO:0000269|PubMed:20484056	1..835		318/945 (34%)
		Vinculin	3..1134	CDD:460040	458/1269 (36%)
		Talin-interaction. /evidence=ECO:0000250|UniProtKB:P12003	168..208		16/39 (41%)
		3 X 112 AA tandem repeats. /evidence=ECO:0000255	259..589		89/341 (26%)
		Interaction with ACTN4. /evidence=ECO:0000269|PubMed:15988023, ECO:0007744|PDB:1YDI	741..764		7/22 (32%)
		Linker (Pro-rich). /evidence=ECO:0000269|PubMed:20484056	836..878		19/59 (32%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	857..887		18/29 (62%)
		C-terminal tail. /evidence=ECO:0000269|PubMed:20484056	879..1134		121/263 (46%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1003..1046		24/42 (57%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1120..1134		7/13 (54%)
deb-1	NP_001293743.1	Vinculin	81..1089	CDD:460040	443/1191 (37%)