Protein Alignment TIAM1 and RhoGEF3

DIOPT Version :10

• Sequence 1: NP_003244.2
• Gene: TIAM1 / 7074
• HGNCID: 11805
• Length: 1591
• Species: Homo sapiens
• Sequence 2: NP_612024.4
• Gene: RhoGEF3 / 38050
• FlyBaseID: FBgn0264707
• Length: 3519
• Species: Drosophila melanogaster
• Alignment Length: 1757
• Identity: 362/1757 - (20%)
• Similarity: 589/1757 - (33%)
• Gaps: 516/1757 - (29%)

Human    17 KHASLGRKHTSRSLRLSHKTRRTRHASSGKVI-HRN-------------SEVSTRSSSTPSIPQS 67
            |..:|||         |.|::.:|..|:|.:| |.|             :..|.|.:|..|..:|
  Fly  1895 KTHALGR---------SRKSQFSRVGSAGPLIAHPNTANVGVSRFRSFDTSKSQRLNSCDSGARS 1950

Human    68 -LAENGLEPFSQDGTLEDFGSPIWVDRVDMGLRPVSYT------DSSVTPSVDSSIVLTAASVQS 125
             |:.:.|:  ::||.|.||.|........:|...||.:      ..|:||..:.|...|::..:.
  Fly  1951 DLSNDELD--NEDGGLNDFLSAGSHKFPKIGTSSVSVSPFKMQRQRSLTPDRNDSHSSTSSLRKQ 2013

Human   126 MPDTEESRLYGDD-----ATYLAEGGRRQHSYTSNGPTFMETASFKKKRSKSAD-----IWREDS 180
            ..:|.|||....:     .:.|:.||.||.| :|...||       :.|.:..|     |.|..|
  Fly  2014 RSNTPESRSLTPEERRKKGSQLSLGGARQSS-SSRSNTF-------EGRQRHDDKTPPNISRSSS 2070

Human   181 LEFSLSDLSQEHL--TSNEEILGSAEEKDCEEARGMETRAS--------PRQLSTCQRAN----- 230
            ........|.|.|  |.:..:..||........|.:..:.:        .|.|...:|:.
  Fly  2071 SSSYSGGESHEPLNGTGSTTVTPSAGSGSTSHRRALTAKQAEQEHRIRRSRSLQLTERSPNRAHK 2135

Human   231 ---SLGDLYAQKNS-GV---TANGGPGSKFAGYCRNLVSDIPNLANHKMPPAAA-----EETP-- 281
               ::|.|..|:.| ||   .:....|..|....|...:......|...||.::     .||.
  Fly  2136 SIVNVGALVPQQRSVGVPYQQSRVPGGGVFPPTIRTSPAGAKIHVNSPAPPNSSVRVRHNETDKA 2200

Human   282 -----PYSNYN-----TLPCRKSHCLSEGATNPQISHSNSMQGRRAKTTQD------VNAGEGSE 330
                 .|:|::     ....|.:|  :.|:.....|:.|....|.:::..|      :|...||.
  Fly  2201 RSFDFDYNNFSRGGGAKGSSRTAH--TSGSGGDSGSNQNLRVERESRSFDDEICEAVLNNNNGSN 2263

Human   331 FADSGIEGATTDTD-----LLSRRSNATNSSYSPTTGRAFVGS----DSGSSSTG--DAARQG-- 382
            .:.||:......||     :.|..|:....|.||.:......|    .||||:..  ..|||.
  Fly  2264 GSVSGLRFLQPATDSGQVSMASTSSSRLRKSNSPVSASGMEASRSPQSSGSSTNNLHQQARQSGS 2328

Human   383 -------------VYE--------NFRR------ELEMSTTNSESLEEAGSAHSDEQSSGT---- 416
                         .||        ||||      ||.:...|.|::...|::..:..|:.|
  Fly  2329 PQSYGTRLCDHELTYEMLRKSPIMNFRRGDSGDYELPVMLRNRETINSGGNSELNFMSNETRIYE 2393

Human   417 ------------LSSPG-QSDILLTAAQGT---------VRKAGALAVK-NFLVHKKNKKVESAT 458
                        ..||| :.|:.|..|.|.         ..:..:.:|| .||.....|   |.|
  Fly  2394 HPTTVLKSQRSLRQSPGSRDDLNLEVAVGVGGDYIYRQPANQPRSSSVKAEFLTTATTK---SCT 2455

Human   459 RR-KWKHYWVSLKGCTLFFYESDGRSGIDHNSIP--------KHAVWVENSIVQAVP-------E 507
            :. ....||.....|.......:.:.|...:..|        .|...|..:...|||       |
  Fly  2456 KTLDTCDYWPRCAACQHKSVSGELKKGPSASGKPLTVLDNVQPHVTSVIVNEDNAVPGPQLRSLE 2520

Human   508 HPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACATAVARHHHKEDTLRLLKSEIKKLEQ 572
            .....:.|..|   |.|.:.|...|||.:      ||....|.     :||.:      |:|::
  Fly  2521 KSNPSWTFLCS---GIASICQPVQQTETD------HSTAQEAA-----EEDIV------IRKVK- 2564

Human   573 KIDMDEKMKKMGEMQ-LSSV------------TDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCY 624
                ..|.::.|..: |.||            ..|.:..|..|......|.::..........|.
  Fly  2565 ----PNKSRRTGRSRSLPSVMLRRLYLAGMPKPGSNEMGTTSDCAASLSQQIKAVSSPTLSEPCL 2625

Human   625 LASLQGGELPNPKRLLAFASRPTKVAMGRLGIF-----SVSSFH--------ALVAARTGETGVR 676
            ....|.|     ......|:..:....|.||:.     ::::|:        .|..|..|....:
  Fly  2626 EEQPQKG-----TESTDVANTGSSAGSGGLGVLQKFKRTLNNFNNKNQLHISPLSPAAVGNNAPK 2685

Human   677 RRTQAMSRSA---------------------SKRRSRFSSL-WGLDTTSKKKQGRPSINQVFGEG 719
            .:|...:.:|                     |..:.||..| |    .|.|::.:...|:
  Fly  2686 TKTSPTTTTASIAPMITTTTASDPGSEAGDTSSGKYRFGPLIW----RSSKERRKTKYNR----- 2741

Human   720 TEAVKKSLEGIFDDIVPDGKREKEVVLPNVHQHNPDCDIWVHEYFTPSWFCLPNNQPALTVVRPG 784
            .:.......||..::..|.:..:..:.             |.....|..|.|.|....:
  Fly  2742 RDKCNSGDSGIQIELEQDEQYSRATMA-------------VSRQDEPRAFALTNGSCGV------ 2787

Human   785 DTARDTLELICKTHQLDHSAHYLRLKFLIENKMQLYVPQPEEDIYELLYKEIEICPKVTQSIHIE 849
              |...:..|.:|:....|:  :...|:::.|:..::...|.|..|  .|..|..|  |:|:
  Fly  2788 --ALSKMRAIRRTNSAKASS--ILGPFIVKTKIAKHLNIGESDKAE--RKAPESLP--TRSL--- 2841

Human   850 KSDTAADTYGFSLSSVE----------EDGIRRLYVNSVKE------TGLASKKGLKAGDEILEI 898
            ......::||.....:|          |:.| ..|..:..|      .|...:.||:.| .::||
  Fly  2842 SQPNGLESYGMGRPDLEDSDSDSVASNEEAI-SFYPTTYAEVLYNFTAGGPQELGLERG-MLIEI 2904

Human   899 NNRAADAL--------NSSMLKDFLSQPSLGLLVRTYPELEEGVELLESPPHRVDGPADLGESPL 955
            ..:.....        .:::::|.|. |.||    .:|  :|.|.::..|           |:..
  Fly  2905 LRKEVGPWWFGRIKKEETNLVEDILG-PELG----WFP--KEFVRIIHCP-----------ETDN 2951

Human   956 AFLTSNPGHSLCSEQGSSAETAPEETEGPDLESSDETDHSSKSTEQVAAFCRSLHEMNPSDQS-- 1018
            .|:    .|...:::.::.|......||             .....||||.........:|||
  Fly  2952 FFI----AHRAAADEAAAEEATARAEEG-------------SVPVPVAAFAEDTDVTVTTDQSNV 2999

Human  1019 -----PSPQDSTGPQLATMRQLSDADKLRK-VICELLETERTYVKDLNCLMERYLKPLQKE-TFL 1076
                 .||...:.|.:..:.|...:..:|: .:.|||:||..|||.|..:.:.||..:.|. ...
  Fly  3000 TLIVIESPPTPSAPSVDFVAQPDHSTIIRRSAVRELLDTEVNYVKLLAAICDGYLPAMSKRIDIF 3064

Human  1077 TQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLFSLGGSFLYYADRFKL 1141
            :.:.:.::|.|:|.:.:||.:||:.|..|:          :.:|..||...:...||       .
  Fly  3065 SPNSIRLIFSNITAIYKFQRKFLEALRRGI----------EQNQVAKVFLKMHKGFL-------C 3112

Human  1142 YSAFCASHTKVPKVLVKAKT-DTAFKAFLDAQNPKQQHSST---LESYLIKPIQRILKYPLLLRE 1202
            ||.:|.::   |:.|::.:| |....|....:|.::..:..   |.::|:.|:|||.:|||.|.|
  Fly  3113 YSTYCNAY---PRALIELETYDRVKDARTILENCRESQNLAELPLSAHLLAPVQRICRYPLHLNE 3174

Human  1203 LF--AL-------------------TDAESEEHYHLDV---------AIKTMNKVASHINEMQKI 1237
            :.  ||                   ||.|..:...||:         |::.|..:...:|| .|.
  Fly  3175 IIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQDTVNLALEAMRGITEAVNE-GKR 3238

Human  1238 HEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTTVIWLNPPASLGK---WKKEPELAAFVFKTA 1299
            |.|       .||.   .:....:.....|.||:...:|...|:..|   |.....|  |:|...
  Fly  3239 HSE-------TIAR---HQASFQNFKGPPLHLHSARFFLQVDATRQKQNLWNSSYTL--FLFDNQ 3291

Human  1300 VV-----------LVYK--------------DGSKQKKKLVG---SHRLSIYED----WDPFRFR 1332
            :|           .:||              ||     |:.|   .:.|.||.:    |..|.||
  Fly  3292 LVYCKRDIIKRSHFIYKGRIFLDRCRVVNVRDG-----KMFGHTIKNSLRIYSESRDKWYDFSFR 3351

Human  1333 ------HMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAV- 1390
                  ..:.|.||:    .....:|..|.|:...:...|.||.   .....|....:||..|.
  Fly  3352 SANRKHRFLSTLALE----RQFGGKALYVSEMTGFEYNYEERPG---DYSDQSDYDAQDFELATG 3409

Human  1391 ----HSILRDKHRRQLLK-TESLPSSQQYVPFGGKRLCALKGARPAMSRA------VSAPSKSLG 1444
                .|.|.:...:...: .|:||...|              :|..:|.|      .:..:.|||
  Fly  3410 VTSGESSLPESPAKSTSRLCETLPKKSQ--------------SRDGISSAENSQIVTTTSTGSLG 3460

Human  1445 RR 1446
            ||
  Fly  3461 RR 3462

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
TIAM1	NP_003244.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..78		19/75 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	298..379		22/97 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	393..422		8/45 (18%)
		PH1_Tiam1_2	432..558	CDD:269937	30/151 (20%)
		Tiam_CC_Ex	572..669	CDD:408184	18/122 (15%)
		Ubl1_cv_Nsp3_N-like	766..832	CDD:475130	13/65 (20%)
		PDZ	858..>901	CDD:214570	13/58 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	939..1034		17/101 (17%)
		RhoGEF	1044..1233	CDD:214619	55/223 (25%)
		PH2_Tiam1_2	1235..1406	CDD:269957	44/217 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1456..1482
RhoGEF3	NP_612024.4	SH3	2876..2942	CDD:214620	14/73 (19%)
		RhoGEF	3028..3234	CDD:238091	56/225 (25%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	30/153 (20%)