Protein Alignment BRAF and PKC1

DIOPT Version :10

• Sequence 1: NP_001361187.1
• Gene: BRAF / 673
• HGNCID: 1097
• Length: 807
• Species: Homo sapiens
• Sequence 2: NP_009445.2
• Gene: PKC1 / 852169
• SGDID: S000000201
• Length: 1151
• Species: Saccharomyces cerevisiae
• Alignment Length: 977
• Identity: 193/977 - (19%)
• Similarity: 322/977 - (32%)
• Gaps: 316/977 - (32%)

Human    40 DPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSI---YLEAYEEYTSKLDALQQREQQLLE 101
            |..||.|..|   .|::|      :.||......|.:|   .|....|...|..|.|..|||...
Yeast   260 DFQIPVEKGN---EIEIT------VYDKVNDSLIPVAIMWLLLSDIAEEIRKKKAGQTNEQQGWV 315

Human   102 SLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQR 166
            :..|....|..:|....|:||:.|:|.....|:.:|....|..::.:..|     ..|..|:.|
Yeast   316 NASNINGGSSLASEEGSTLTSTYSNSAIQSTSAKNVQGENTSTSQISTNS-----WFVLEPSGQ- 374

Human   167 TVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVP 231
              :....|......:::..:|.||......:.|.:                       |:.|.
Yeast   375 --ILLTLGFHKSSQIERKQLMGGLHRHGAIINRKE-----------------------EIFEQ-- 412

Human   232 LTTHNFVRKTFFTLAFCDFCRKLL-FQGFRCQTCGYKFHQRCSTEVPLMCV-------NYDQLDL 288
             ..|:||:|:|:.:..|.:|...| :.||:||.|.:..|::|.|.|...|:       :.|:..|
Yeast   413 -HGHHFVQKSFYNIMCCAYCGDFLRYTGFQCQDCKFLCHKKCYTNVVTKCIAKTSTDTDPDEAKL 476

Human   289 LFVSKFFEHHPIPQE-------------------------------------------------- 303
                    :|.||..
Yeast   477 --------NHRIPHRFLPTSNRGTKWCCHCGYILPWGRHKVRKCSECGIMCHAQCAHLVPDFCGM 533

Human   304 EASLAETALTS------------GSSPSAPASDSIGPQILTSPSPSK-----SIPIPQPFRPADE 351
            ...:|...|.:            .:.|||....|||....:..||||     :.|:|...|..|:
Yeast   534 SMEMANKILKTIQDTKRNQEKKKRTVPSAQLGSSIGTANGSDLSPSKLAERANAPLPPQPRKHDK 598

Human   352 --------------------DHR-----------NQF-------------GQRDRSSSAPNVHIN 372
                                |.|           |:|             .|:....|:|.   .
Yeast   599 TPSPQKVGRDSPTKQHDPIIDKRISLQTHGREKLNKFIDENEAYLNFTEGAQQTAEFSSPE---K 660

Human   373 TIEPV--------------------NIDDLIRDQ-------------GFRGDGAPL--------- 395
            |::|.                    :.|||:||:             ..:.|...:
Yeast   661 TLDPTSNRRSLGLTDLSIEHSQTWESKDDLMRDELELWKAQREEMELEIKQDSGEIQEDLEVDHI 725

Human   396 -------------NQLMRCLRKYQSRTPSPL--LHSVPSEIVFDFEPGPVFRGSTTGLSATPPAS 445
                         |..........|...:|.  ::|...:|..|.....|.: .|..|:   |.|
Yeast   726 DLETKQKLDWENKNDFREADLTIDSTHTNPFRDMNSETFQIEQDHASKEVLQ-ETVSLA---PTS 786

Human   446 LPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGT 510
            ...|.|..   |:||     :||.:|:..:::.:...||..|.|      ...:.:.:|.|:||.
Yeast   787 THASRTTD---QQSP-----QKSQTSTSAKHKKRAAKRRKVSLD------NFVLLKVLGKGNFGK 837

Human   511 VYKGKWHGD---VAVKMLN----VTAPTPQQLQAFKNEVGVLRKTRHVNIL-LFMGYSTKPQLAI 567
            |...|....   .|:|:|.    :.....:..:|.|....:..||:|..:. |:..:.|:.::..
Yeast   838 VILSKSKNTDRLCAIKVLKKDNIIQNHDIESARAEKKVFLLATKTKHPFLTNLYCSFQTENRIYF 902

Human   568 VTQWCEGSSLYHH-----LHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHED 627
            ..::..|..|..|     |.:...||       .|.:....:.|.|...:|:||||..||.|..:
Yeast   903 AMEFIGGGDLMWHVQNQRLSVRRAKF-------YAAEVLLALKYFHDNGVIYRDLKLENILLTPE 960

Human   628 LTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTG 692
            ..:||.|:||.. ...|.| ::.....|:..:||||:::.|:   |:...|.:|||::||:::..
Yeast   961 GHIKIADYGLCK-DEMWYG-NRTSTFCGTPEFMAPEILKEQE---YTKAVDWWAFGVLLYQMLLC 1020

Human   693 QLPYSNINNRDQI------------------IFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKK 739
            |.|:|. ::.|::                  |..:.:|.|:.|..|.....|:....:|.|...:
Yeast  1021 QSPFSG-DDEDEVFNAILTDEPLYPIDMAGEIVQIFQGLLTKDPEKRLGAGPRDADEVMEEPFFR 1084

Human   740 K---------RDERPLFPQI------------LASIELLARSLPKIHRSASEPSLNRAGFQTEDF 783
            .         |.:.|..|:|            ..|.......||.:..::.:.......|..:|.
Yeast  1085 NINFDDILNLRVKPPYIPEIKSPEDTSYFEQEFTSAPPTLTPLPSVLTTSQQEEFRGFSFMPDDL 1149

Human   784 SL 785
            .|
Yeast  1150 DL 1151

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
BRAF	NP_001361187.1	RBD_BRAF	154..232	CDD:340654	10/77 (13%)
		C1_B-Raf	230..289	CDD:410421	19/66 (29%)
		STKc_Raf	503..755	CDD:270964	70/303 (23%)
PKC1	NP_009445.2	HR1_PKC-like_1_fungi	1..72	CDD:212011
		HR1_PKC-like_2_fungi	110..181	CDD:212010
		C2_fungal_Pkc1p	211..319	CDD:176071	19/67 (28%)
		C1_ScPKC1-like_rpt1	413..464	CDD:410372	18/50 (36%)
		C1_ScPKC1-like_rpt2	478..536	CDD:410373	3/57 (5%)
		STKc_PKC	828..1145	CDD:270722	72/329 (22%)