Protein Alignment BRAF and ksr

DIOPT Version :10

• Sequence 1: NP_001361187.1
• Gene: BRAF / 673
• HGNCID: 1097
• Length: 807
• Species: Homo sapiens
• Sequence 2: NP_524236.2
• Gene: ksr / 40660
• FlyBaseID: FBgn0015402
• Length: 966
• Species: Drosophila melanogaster
• Alignment Length: 916
• Identity: 243/916 - (26%)
• Similarity: 374/916 - (40%)
• Gaps: 271/916 - (29%)

Human    50 IKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFS---- 110
            ::|.::|:.|.|..||      .:.||   :..||...:|....|..::.:|||.:||..|
  Fly   128 LEQSLRLSDEEIRQLL------ADSPS---QREEEELRRLTRAMQNLRKCMESLESGTAASNNDP 183

Human   111 -------------------------VSSSASMDT-----VTSSSSSSLSVLPSSLSVFQNPTDVA 145
                                     .:|:||..|     .....:|:|:...:..|..|:|:...
  Fly   184 EQWHWDSWDRPTHIHRGSVGNIGLGNNSTASPRTHHRQHGVKGKNSALANSTNFKSGRQSPSATE 248

Human   146 RSN------------PKSPQKPIVRVFLPNK----------QR---TVVPARCGVTVRDSLKKAL 185
            ..|            |..|..|    |.|:.          ||   |..|||...|:   |.::
  Fly   249 ELNSTQGSQLTLTLTPSPPNSP----FTPSSGLSSSLNGTPQRSRGTPPPARKHQTL---LSQS- 305

Human   186 MMRGLIPECCAVYRIQ-DGE-----KKPIGWDTDISWLTGEELHVEVLEN------------VPL 232
                         .:| |||     :.|.....|....|..::.|:...|            ||.
  Fly   306 -------------HVQVDGEQLARNRLPTDPSPDSHSSTSSDIFVDPNTNASSGGSSSNVLMVPC 357

Human   233 T------------THNFVRKTFFTLAFCDFCRKLLF-QGFRCQTCGYKFHQRCSTEVPLMCVNYD 284
            :            .|.|.:...| :|.|..|:|.:| :..:|..|.|..|:.|:..||..|
  Fly   358 SPGVGHVGMGHAIKHRFTKALGF-MATCTLCQKQVFHRWMKCTDCKYICHKSCAPHVPPSC---- 417

Human   285 QLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAP--ASDSIG----PQILTSPSPSKSIPIP 343
            .|...:|.:|  .|  .:|:...|......|::..:|  ...::|    .|...|.|||.|....
  Fly   418 GLPREYVDEF--RH--IKEQGGYASLPHVHGAAKGSPLVKKSTLGKPLHQQHGDSSSPSSSCTSS 478

Human   344 QPFRPADEDHRNQFGQRDR-------SSSA------------------PNVHINTI--------- 374
            .|..||      .|.||:|       ||||                  |||.:.:.
  Fly   479 TPSSPA------LFQQRERELDQAGSSSSANLLPTPSLGKHQPSQFNFPNVTVTSSGGSGGVSLI 537

Human   375 --EPV-------------NIDDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPS-------PLLHSV 417
              |||             .:|.|:...         |..|..|...|:...|       .|::|.
  Fly   538 SNEPVPEQFPTAPATANGGLDSLVSSS---------NGHMSSLIGSQTSNASTAATLTGSLVNST 593

Human   418 PSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLG 482
            .:.....|.|..:   ||.|:....|.:...:.|:    :.:...:....|.|:|.|.:|  |..
  Fly   594 TTTSTCSFFPRKL---STAGVDKRTPFTSEYTDTH----KSNDSDKTVSLSGSASTDSDR--TPV 649

Human   483 RRDSSDD---------------WEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVT-APT 531
            |.||::|               |:||.|.:.:.:|||.|.||||::..||||||||:||.. ...
  Fly   650 RVDSTEDGDSGQWRQNSISLKEWDIPYGDLLLLERIGQGRFGTVHRALWHGDVAVKLLNEDYLQD 714

Human   532 PQQLQAFKNEVGVLRKTRHVNILLFMG-YSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLID 595
            ...|:.|::||...:.|||.|::|||| ....|.|||||..|:|::||.::|....||.|.:.:.
  Fly   715 EHMLETFRSEVANFKNTRHENLVLFMGACMNPPYLAIVTSLCKGNTLYTYIHQRREKFAMNRTLL 779

Human   596 IARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLAT------------VKSRWSGSH 648
            ||:|.||||.||||:.|||:||::.|||: |:..|.|.||||.:            |...|
  Fly   780 IAQQIAQGMGYLHAREIIHKDLRTKNIFI-ENGKVIITDFGLFSSTKLLYCDMGLGVPHNW---- 839

Human   649 QFEQLSGSILWMAPEVIR-MQDKNP------YSFQSDVYAFGIVLYELMTGQLPYSNINNRDQII 706
                    :.::|||:|| :|.:.|      ::..||||:||.|.|||:.|:..:.: ...:.||
  Fly   840 --------LCYLAPELIRALQPEKPRGECLEFTPYSDVYSFGTVWYELICGEFTFKD-QPAESII 895

Human   707 FMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEP 771
            :.|||| :...|:.::|.  :.:|.|:..|...:::.||.|.::|:.:|.|.:.  ::.||.|.|
  Fly   896 WQVGRG-MKQSLANLQSG--RDVKDLLMLCWTYEKEHRPQFARLLSLLEHLPKK--RLARSPSHP 955

Human   772 -SLNRA 776
             :|:|:
  Fly   956 VNLSRS 961

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
BRAF	NP_001361187.1	RBD_BRAF	154..232	CDD:340654	21/108 (19%)
		C1_B-Raf	230..289	CDD:410421	19/71 (27%)
		STKc_Raf	503..755	CDD:270964	104/272 (38%)
ksr	NP_524236.2	SAM_KSR1_N	33..86	CDD:466555
		SAM_KSR1	92..166	CDD:463916	12/46 (26%)
		C1_KSR	370..418	CDD:410362	16/52 (31%)
		PK_KSR	678..946	CDD:270965	107/284 (38%)