Protein Alignment BRAF and lin-45

DIOPT Version :9

• Sequence 1: NP_001361187.1
• Gene: BRAF / 673
• HGNCID: 1097
• Length: 807
• Species: Homo sapiens
• Sequence 2: NP_001379593.1
• Gene: lin-45 / 177436
• WormBaseID: WBGene00003030
• Length: 855
• Species: Caenorhabditis elegans
• Alignment Length: 821
• Identity: 312/821 - (38%)
• Similarity: 419/821 - (51%)
• Gaps: 161/821 - (19%)

Human   108 DFSVSSSASMDTVTSSSSSSLSVLP--SSLSVFQNPTDVARSNPKSPQKP--------------- 155
            :|..||:::..|.....|..|..||  :|.|:.:.......::|.:|..|
 Worm    47 NFKKSSASTTPTSPHCPSPRLISLPRCASSSIDRKDQASPMASPSTPLYPKHSDSLHSLSGHHSA 111

Human   156 ----------------IVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGE 204
                            ::.|.||..|.:.|..|.|.|.||::.|.|..|.:.|:.|.|....|.:
 Worm   112 GGAGTSDKEPPKFKYKMIMVHLPFDQHSRVEVRPGETARDAISKLLKKRNITPQLCHVNASSDPK 176

Human   205 KKPIGWDTDI----SWLTGEEL--HVEVLENVPLTTHNFVRKTFFTLAFCDFCRK-LLFQGFRCQ 262
            ::.|.....:    |.|.|.||  |.|.|..|....|..||:||.....||.|.. :...||||:
 Worm   177 QESIELSLTMEEIASRLPGNELWVHSEYLNTVSSIKHAIVRRTFIPPKSCDVCNNPIWMMGFRCE 241

Human   263 TCGYKFHQRCSTEVPLMC-------VNYDQLDLLF-VSKFFEHHPIPQEEASLAETALTSGSSPS 319
            .|.:|||||||:..||.|       .|.|.:..|| ::...|.......|..||..|.|||.||:
 Worm   242 FCQFKFHQRCSSFAPLYCDLLQSVPKNEDLVKELFGIASQVEGPDRSVAEIVLANLAPTSGQSPA 306

Human   320 APASDS-------------------IGPQILTSP-SPSKSIPIPQPFRPADEDHRNQFGQRDRSS 364
            |....|                   :.||..||. |||...|                  |||||
 Worm   307 ATPDSSHPDLTSIKRTGGVKRHPMAVSPQNETSQLSPSGPYP------------------RDRSS 353

Human   365 SAPNVH--------------INTIEPVNIDDLIRDQ-----------------GFRGDGAPLNQ- 397
            ||||::              ::.:|...:::..||:                 |....||.:|:
 Worm   354 SAPNINAINDEATVQHNQRILDALEAQRLEEESRDKTGSLLSTQARHRPHFQSGHILSGARMNRL 418

Human   398 --LMRCLRKYQSRTPSPLLHSVPSEIVFDFEPG--PVFRGSTTG---------LSATPPASLPGS 449
              |:.| ....|.:||....|.|..::.....|  |...||||.         |..|||.|.|.
 Worm   419 HPLVDC-TPLGSNSPSSTCSSPPGGLIGQPTLGQSPNVSGSTTSSLVAAHLHTLPLTPPQSAPP- 481

Human   450 LTNVKALQK-SPGPQRERKSSSSSE-DRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVY 512
                   || |||..|.|..|.... |..|.:. .::...:||||...:..:..::|||||||||
 Worm   482 -------QKISPGFFRNRSRSPGERLDAQRPRP-PQKPHHEDWEILPNEFIIQYKVGSGSFGTVY 538

Human   513 KGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSL 577
            :|::.|.||:|.|||..|||.|:.||||||.||:||||:|:|||||:..:|::||:|||||||||
 Worm   539 RGEFFGTVAIKKLNVVDPTPSQMAAFKNEVAVLKKTRHLNVLLFMGWVREPEIAIITQWCEGSSL 603

Human   578 YHHLHIIE--TKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDL-TVKIGDFGLAT 639
            |.|:|:.|  .:|||..:|||.:|.:.||:|||:|:|||||||:|||||.:|: |||||||||||
 Worm   604 YRHIHVQEPRVEFEMGAIIDILKQVSLGMNYLHSKNIIHRDLKTNNIFLMDDMSTVKIGDFGLAT 668

Human   640 VKSRW--SGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNR 702
            ||::|  :|..|.:|.:|||||||||||||||.|||:.|||||:|||.:||:::..|||||||||
 Worm   669 VKTKWTVNGGQQQQQPTGSILWMAPEVIRMQDDNPYTPQSDVYSFGICMYEILSSHLPYSNINNR 733

Human   703 DQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRS 767
            |||:||||||||.||.||:|.:.||:|.:|...|:...|:|||:|.::|..:..:.  |||:.||
 Worm   734 DQILFMVGRGYLRPDRSKIRHDTPKSMLKLYDNCIMFDRNERPVFGEVLERLRDII--LPKLTRS 796

Human   768 ASEPSLNRAGFQTE-----------DFSLYACASPKTPIQA 797
            .|.|::.....|..           .:|....|:.|||..|
 Worm   797 QSAPNVLHLDSQYSVMDAVMRSQMLSWSYIPPATAKTPQSA 837

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
BRAF	NP_001361187.1	RBD_BRAF	154..232	CDD:340654	28/114 (25%)
		C1_B-Raf	230..289	CDD:410421	26/66 (39%)
		STKc_Raf	503..755	CDD:270964	162/256 (63%)
lin-45	NP_001379593.1	RBD	128..203	CDD:128731	24/74 (32%)
		C1_Raf	211..260	CDD:410361	22/48 (46%)
		STKc_Raf	529..786	CDD:270964	162/256 (63%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	340	1.000	Domainoid score	I636
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H3197
Inparanoid	1	1.050	435	1.000	Inparanoid score	I1045
Isobase	1	0.950	-	0	Normalized mean entropy	S2247
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D129822at33208
OrthoFinder	1	1.000	-	-		FOG0001587
OrthoInspector	1	1.000	-	-		otm15683
orthoMCL	1	0.900	-	-		OOG6_100006
Panther	1	1.100	-	-	LDO	PTHR44329
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X994
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	11	10.920