Protein Alignment SPTBN2 and shot

DIOPT Version :10

• Sequence 1: NP_001397954.1
• Gene: SPTBN2 / 6712
• HGNCID: 11276
• Length: 2397
• Species: Homo sapiens
• Sequence 2: NP_725339.1
• Gene: shot / 36542
• FlyBaseID: FBgn0013733
• Length: 8805
• Species: Drosophila melanogaster
• Alignment Length: 2508
• Identity: 517/2508 - (20%)
• Similarity: 911/2508 - (36%)
• Gaps: 622/2508 - (24%)

Human   166 RFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLL 230
            |.:|.||.|..|||   .||::|||.|.:..||.||.|.|::||:||||||||:|:||::|||||
  Fly   208 RREISDIVVGKEDN---VSAREALLRWARRSTARYPGVRVNDFTSSWRDGLAFSALVHRNRPDLL 269

Human   231 DFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAV 295
            |:...:.......|:.||::.|||.|:|:|||||||:.::|||||:|||:::.|..|.
  Fly   270 DWRKARNDRPRERLETAFHIVEKEYGVTRLLDPEDVDTNEPDEKSLITYISSLYDVFP------- 327

Human   296 EGKRIGKVLDHAMEAERLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTV 360
            |...|..:.|  ||::|.|.:|..||.:.:.|..:....|.:|....:|..::..|.....:|:.
  Fly   328 EPPSIHPLFD--MESQRRVHEYRDLAQQFIYWCREKTAYLQERSFPPTLIEMKRLLSDLQRFRSD 390

Human   361 EKPPKFTEKGNLEVLLFTIQSKLRANNQKV-YTPREGRLISD-INKAWERLEKAEHERELALRTE 423
            |...:..||..    |..|..:|....:.| ....|..|..| |.|||.|:..|..:||:.|:.|
  Fly   391 EVSARKREKSK----LIQIYKELERYFETVGEVDVEAELRPDAIEKAWYRMNTALQDREVILQQE 451

Human   424 LIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRV 488
            :.|.|:|::||.:..|:....:..|::.:..:.::...:|......|....||:||:|......:
  Fly   452 IERLERLQRLADKVQREIKHVDQKLTDLEGRIGEEGRRIERLHPVDAKSIVEALETEIRHLEEPI 516

Human   489 QAVDAVAAELAAERYHDIKRIAARQHNVARLWDFLR-------------------QMVAARRERL 534
            |.::.....|...||..:..:..:.:.:.:.|..||                   :....|:.|:
  Fly   517 QDMNQDCHVLNEGRYPHVSELHKKVNKLHQRWAQLRTNFHTNLVQKLSGLKYPVHETTVTRQTRM 581

Human   535 LL---NLELQKVFQDLLYLMDWMEEMKGRLQSQDLGRHLAGVEDLL--QLHELVEADIAVQAERV 594
            ::   .::....|:||...::|.:....:|.:.|.|..|..|::.|  |.||            .
  Fly   582 VVESRQIDTNPHFRDLQEHIEWCQNKLKQLLAADYGSDLPSVKEELDRQQHE------------H 634

Human   595 RAVSASALRFCNPGKE---YRPCDPQLVSERVAKLEQSYEALCELAAARRARLEESRRLWRFLWE 656
            :.:.....:..|..::   :...:..|..:|:.:|::.|   .||.:....||.:...|..||.:
  Fly   635 KIIDQFHTKILNDERQQTKFSGDELALYQQRLNQLQKVY---AELLSTSTKRLSDLDSLQHFLGQ 696

Human   657 VGEAEAWVREQQHLLAS---ADTGRDLTGALRLLNKHTALRGEMSGRLGPLKLTLEQGQQLVAEG 718
            ......|:.|::.:..:   ||...||....|.   :..|..|:..|.......|::|:.|:.:.
  Fly   697 ASAELQWLNEKEQVEITRDWADKQLDLPSVHRY---YENLMSELEKREMHFATILDRGEALLNQQ 758

Human   719 HPGASQASARAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLVSS---- 779
            ||.:....|....||.||..|..|.......|..|...:||..:..|.|.||.....:::|
  Fly   759 HPASKCIEAHLTALQQQWAWLLQLTLCLEVHLKHATEYHQFFGEIKDAEQWLAKRDEILNSKFSQ 823

Human   780 PELGHDEFSTQALARQHRALEEEIRSHRPTLDALREQAAALPPTLSRTPEVQSRVPT----LERH 840
            .:.|.|:..|  |.|..:.|.||:.:...|:..|:.:|..:.|...|...|..:.|.    ..:.
  Fly   824 SDFGLDQGET--LLRGMQDLREELNAFGETVATLQRRAQTVVPLNKRRQPVNRQGPVQAICAYKQ 886

Human   841 YEELQARAGERARALEAALALYTMLSEA--------GACGLWVEEKEQWLNGLALPERLED---- 893
            ..:||...||....|:.:..:...:..|        |||.|.....::.::.....:||.|
  Fly   887 QGQLQIEKGETVTLLDNSGRVKWRVRTAKGQEGPIPGACLLLPPPDQEAIDAAERLKRLFDRSVA 951

Human   894 -----------------LEVVQ----QRFETLEPEMNTLAAQITAVNDIAEQLLK---ANPPGKD 934
                             :.||:    .:|..:.||..|...:  |:||.|::||.   .|.|...
  Fly   952 LWQKKHLRLRQNMIFATIRVVKGWDFDQFLAMGPEQRTAIRR--ALNDDADKLLSEGDPNDPQLR 1014

Human   935 RIVNTQEQLNHRWQQFRRLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIESTQGLGNDLA 999
            |:....:::|..:.:|.:.|   :|...|..:.:.:..||
  Fly  1015 RLRREMDEVNRLFDEFEKRA---RAEEESKQASRIFTEEC------------------------- 1051

Human  1000 GVLALQRKLAGTERDLEAIAARVGELTREANALAAGHPAQAVAINARLREVQTGWEDLRATMRRR 1064
              ||::.||....|:|:.|.  :..|.|:.::|            ..:.|:.:.:|      ||.
  Fly  1052 --LAIKSKLEDMARELDQII--LAPLPRDLDSL------------EHVLEIHSDYE------RRL 1094

Human  1065 EESLGEARRLQDFLR-----------SLDD-FQAW-------------LGRTQTAVAS-EEGPAT 1103
            .....|.:.||:..|           |||: .:.|             |...:.::|. |:....
  Fly  1095 HLLEPELKHLQETFRTIALKTPVLKKSLDNLMELWKELNTQSGLHKDRLKLLEASLAGLEDNEHV 1159

Human  1104 LPEAEALLAQHAALRGEVERAQSEYSRLRALGEEVTR---------DQAD-------PQCLF--- 1149
            :.|.|..||:|..|....|..|..:.:|..:.:.:|:         |.||       |..:.
  Fly  1160 ISELENELARHQDLPSTAEGLQQVFKQLNHMQDIITQQQPQMDKMNDAADQLGRMGVPTKVLGDL 1224

Human  1150 --LRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQ 1212
              |...:|.|.|.|..:......|......|.|....|:.:.|.|     :.:|...||....:.
  Fly  1225 KRLHSNVERLNTRWSAVCNQLGERMRSCETAIGLMKNLQSSVQVE-----ESWVDGTTERLSAMP 1284

Human  1213 AADAAIKKLEDFMSTMDANGERIHGLLEAGRQLVSEGNIHADKIREKAD---------SIERRHK 1268
            .|.:|. :|:..:........:|..:..||.:|:.|..|:..|.....|         |..||..
  Fly  1285 TATSAY-ELDKLLGAAIERKPKIENVNVAGGRLIREAKIYDSKCLRFVDWLVEARPSFSPPRRDL 1348

Human  1269 KNQD----AAQQFLGRLRD-NREQQHFLQDCHELKLWIDEKMLTAQDVSYDEARNLHTKWQKH-Q 1327
            :..|    |.|.:..||.: |.:....|:.       :.:::.||.:|:..:........||. :
  Fly  1349 RPADSDPGATQYYSQRLDNLNTKNDRLLEQ-------LSQRLKTAIEVNGSDGLQYAESLQKPLK 1406

Human  1328 AFMAELAA----------------------------------------NKDWLDKVDKEGRELTL 1352
            .|..:.:|                                        :.|.||...:|.....|
  Fly  1407 TFRVDFSAGSVPTGDGYAARQEDLYTTTYSTTQISSTKTTKSSTKSVYSSDGLDAASQEVSTAGL 1471

Human  1353 EKPELKALVSEKLRDLHRRWDELETTTQAKARSLFDANRAELFAQSCCALESWLESLQAQLHSDD 1417
            .:.:::   ..::|.|.|       :.|....|:.|.                     |.:....
  Fly  1472 PQSQIQ---FNEIRTLKR-------SQQLGGHSVLDI---------------------AGIRDPR 1505

Human  1418 YGKDLT-SVNILLKKQQMLEWEMAVREKEVEAIQAQAKALAQEDQGAGEVERTSRAVEEKFRALC 1481
            .|:.|| ...|.|:...:...||.|.::.:...||       .|||..:::..        :.|.
  Fly  1506 TGRVLTIGEAIQLRILDVRTGEMLVGDRRITLEQA-------ADQGLIDLQLA--------KQLL 1555

Human  1482 QPMRERCRRLQASREQHQFHRDVEDEILWVTERLPMASSMEHGKDLPSVQLLMKKNQTLQKEIQG 1546
            :|...|    .||..:......::.||          |..|.|.:  :.:..:|.|.|:..|...
  Fly  1556 EPGAGR----DASGRELSLLEVIQREI----------SEAESGYE--TAEKRIKVNNTVTVEQTS 1604

Human  1547 HE------PR-IADLRERQRALGAAAAGP----------------ELAELQEMWKRLGHE---LE 1585
            .|      || |||         |..||.                .|||..|....:.||   ::
  Fly  1605 GELGSPENPRNIAD---------AITAGSVDTKTGLYRVKSGQTISLAEAYERGYLIRHESVTIK 1660

Human  1586 LRGKRLEDALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSAQAEVKKHQVLEQALADYAQT 1650
            .....|.||:.     ....:...|:.::        .:.|:..          |:.|:|:
  Fly  1661 SNALCLSDAIA-----HGLVDGAGWIADR--------NSGDKFR----------LDSAIAN---- 1698

Human  1651 IHQLAASSQDMIDHEHPESTRISIRQAQVDKLYAGLKELAGER--RERLQEHLRLCQLRRELDDL 1713
             ..:.||.::::|.:  ..|:|::::|    |.:|:......|  .|..:|.|...:.|
  Fly  1699 -QLIDASVREVVDAK--RDTKITLQEA----LQSGVLNAKTGRYVNEVTKEKLTFAEAR------ 1750

Human  1714 EQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQERVDSANALANGLIAGGHAARAT 1778
                 .|:::...:.| :|...:.:| ||..:.   ||.:.:|::....|:..|::.|......|
  Fly  1751 -----NRQLIVKPYTL-KDICDLNLL-DKQAQI---TSPMRREKLSIMQAIEAGVLDGNLLKCIT 1805

Human  1779 VAEWK-DSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHK-------QQQLPDGT 1835
            ..:.: .:|.||.||        |.||.|..:.:.|:.|...::.....:       |:.:.|..
  Fly  1806 KRKGELVTLQEAIAD--------GIVLPAECKYRDFMTGELISIPEAVERGLISSVAQRSIFDID 1862

Human  1836 G-RDL--------NAA---EALQRRHC--AYEHDIQALSPQVQQVQDDGHRLQKAYAGDKAEEIG 1886
            | :||        |.|   :.|:|:..  |.|....:|.| ::....:|...|:.|     |...
  Fly  1863 GFKDLRSNDYVSFNVALSRDLLRRKSTGFALETGRDSLVP-LEVAVSEGLVRQEVY-----EMFS 1921

Human  1887 RHMQAVAEAWAQLQGSSAARRQLL-----------------LDTTDKFRFFKAVRELMLWMDEVN 1934
            |.:.....:..:|.........|:                 |||..:.:|......|:| ...:|
  Fly  1922 RGIGVQNASGKELSVFDLVYHNLIDPKTGYLLDPKTGETVPLDTAIERKFITPEGALLL-SSLLN 1985

Human  1935 LQMDAQERPRDVSSADLVIKNQQGIKAEIEARADRFSSCIDMG---------KELLARSHYAAEE 1990
            :.:..:...|.::.   .:..:.|.:..:::....|:..:..|         |:....:.|:.::
  Fly  1986 ITLTTETVTRTINR---YVTIRAGSQEPVDSVLLTFTEAVRQGFIDEERQLFKDPKTGNIYSVQQ 2047

Human  1991 ISEKLSQLQARRQETAEKWQEK--MDWLQLVLEVLVFGRDAGMAEAWLCSQEPL------VRSAE 2047
            .......:....|...|....|  ...:.:|.:.::       .||     ||:      .:..|
  Fly  2048 ALNYGLLVPDSNQTVPEPTNRKKTKSTITIVTKQII-------PEA-----EPIKLNTQHTKYVE 2100

Human  2048 LGCTVDEVESLIKRHEAFQKSAVAWEERFCALEKLTALEEREKERKRKREEEERRKQPPAPEPTA 2112
            ....:...:.|:|.|....:        |..|||.:.:|:...||:                 ..
  Fly  2101 KSVEIPISQELVKPHRVVSE--------FINLEKSSYIEQNVTERQ-----------------IM 2140

Human  2113 SVPPGDLVGGQTASDTTW---DGTQPRP-PPSTQAPSVNGVCTDGEPS-QPLLGQQRLEHSSFPE 2172
            .:|||.           |   |..:.|. .|.|....|.|  ||...: :..:.:|.:.:.|. .
  Fly  2141 ELPPGG-----------WRLKDAIEQRLFNPDTGVFHVQG--TDRLVNFEECINKQIINNLSL-S 2191

Human  2173 GPGPGSGDEANGPRGERQTRTRGPAPSAMPQSRSTESA-HAATLPPRGPEPSAQEQMEGMLCRKQ 2236
            ...|.:||:.                       |.:|| ....|...|...::::|::||.....
  Fly  2192 VIDPNTGDKI-----------------------SVQSAFERDILDSYGNYTNSRKQVQGMRSAID 2233

Human  2237 EMEAFGKKAANRSWQNVYCVLRRGSLGFYKDAKAASAGV----PYHGEV---------PVSLARA 2288
            |.:...:........|...:||...:....|....|..:    |...||         |..|..|
  Fly  2234 ESKIILETVPATRGANQKTILRITKVNNIPDVLEVSTPLKDAPPKFVEVLTCQRELASPEPLQIA 2298

Human  2289 QGSVAFDYRKRKHVFKLGLQDGK-EYLFQAKDEAEMSSWL--------------------RVVNA 2332
            .|:: :|......:|   .|.|: |.:|.|..:..:...|                    .:.:.
  Fly  2299 PGAI-YDPSTALVIF---TQTGETENIFDAARQGLVDEQLIKIVDPTTKQPISVTEAIARSIYDP 2359

Human  2333 AIATASSASGEPEEPVVPSTTRGMTRAMTMPPVSPVGA 2370
            ..||...:.|:|.: ::.:|..|:...:..|.|:..||
  Fly  2360 KTATILDSEGQPVD-LITATKLGLLSVVGAPLVAAEGA 2396

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SPTBN2	NP_001397954.1	CH_SPTB-like_rpt1	52..166	CDD:409095	517/2508 (21%)
		CH_SPTBN2_rpt2	180..298	CDD:409170	57/117 (49%)
		Spectrin	312..421	CDD:395348	29/110 (26%)
		Spectrin	436..529	CDD:395348	16/111 (14%)
		SPEC	541..753	CDD:238103	48/219 (22%)
		SPEC	756..965	CDD:238103	55/252 (22%)
		SPEC	968..1179	CDD:238103	47/257 (18%)
		SPEC	1181..1390	CDD:238103	45/263 (17%)
		SPEC	1391..1595	CDD:238103	45/230 (20%)
		SPEC	1596..1808	CDD:238103	40/214 (19%)
		SPEC	1809..2017	CDD:238103	38/256 (15%)
		SPEC	2025..>2082	CDD:197544	11/62 (18%)
		dnaA	<2104..>2248	CDD:455861	26/149 (17%)
		PH_beta_spectrin	2228..2331	CDD:269975	23/136 (17%)
shot	NP_725339.1	SAC6	204..>420	CDD:227401	87/227 (38%)
		CH_PLEC-like_rpt2	223..327	CDD:409038	55/103 (53%)
		SPEC	471..689	CDD:238103	39/232 (17%)
		SPEC	690..866	CDD:238103	46/180 (26%)
		SH3_10	864..929	CDD:407754	14/64 (22%)
		SPEC	1047..1250	CDD:238103	47/249 (19%)
		SPEC	1153..1390	CDD:238103	52/249 (21%)
		PLEC	<1498..1526	CDD:197605	6/48 (13%)
		Plectin	1949..1980	CDD:459901	5/30 (17%)
		DUF5585	2860..>3198	CDD:465521
		SMC_prok_B	<4936..>5564	CDD:274008
		SPEC	5304..5513	CDD:238103
		SPEC	<5658..5787	CDD:413338
		SPEC	5793..6007	CDD:238103
		SPEC	6011..6220	CDD:238103
		SPEC	6118..6330	CDD:238103
		PRK09039	<6279..6441	CDD:181619
		SPEC	6443..6660	CDD:238103
		SPEC	6663..6878	CDD:238103
		SPEC	6880..7087	CDD:238103
		SPEC	6986..7196	CDD:238103
		SPEC	7196..7417	CDD:238103
		SPEC	7314..7526	CDD:238103
		SPEC	7528..7747	CDD:238103
		SPEC	7749..7968	CDD:238103
		SPEC	7973..8181	CDD:238103
		FRQ1	<8375..8444	CDD:444056
		GAS2	8450..8522	CDD:128539
		PHA03307	8550..>8795	CDD:223039